EXPERIMENTAL AND THERAPEUTIC MEDICINE 8: 1723-1726, 2014
Effect of Avastin on the number and structure of tumor blood vessels of nude mice with A549 lung adenocarcinoma NALI ZHANG1,2, GUOJUN ZHANG1, YOUGUANG ZHENG2, TONGBING WANG2 and HONGLEI WANG3 1
Department of Respiratory Medicine, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, Henan 450003; Department of Respiratory Medicine, Luoyang Central Hospital Affiliated to Zhengzhou University, Luoyang, Henan 471009; 3 Department of Cardiovascular Medicine, The First Affiliated Hospital of Henan University of Science and Technology, Luoyang, Henan 471003, P.R. China
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Received March 5, 2014; Accepted August 15, 2014 DOI: 10.3892/etm.2014.1991 Abstract. The aim of the present study was to investigate the effect of Avastin on the number and structure of tumor blood vessels of nude mice with A549 lung adenocarcinoma. A total of 30 nude mice were randomly divided into three groups, namely the control, the Avastin I (Avastin 3 mg̸kg) and the Avastin II (Avastin 6 mg/kg) groups. Following treatment, ELISA was used to detect the expression level of vascular endothelial growth factor (VEGF) in tumor tissues. The microvascular density in tumor tissues and tumor vascular pericyte coverage was detected by immunofluorescence. The tumor growth and survival rate of mice in the three groups were also analyzed. Compared with the control group, the Avastin I and II groups exhibited significantly decreased VEGF levels and microvascular density in the tumor tissues, with the decrease in the Avastin II group being more prominent (P
Effect of Avastin on the number and structure of tumor blood vessels of nude mice with A549 lung adenocarcinoma NALI ZHANG1,2, GUOJUN ZHANG1, YOUGUANG ZHENG2, TONGBING WANG2 and HONGLEI WANG3 1
Department of Respiratory Medicine, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, Henan 450003; Department of Respiratory Medicine, Luoyang Central Hospital Affiliated to Zhengzhou University, Luoyang, Henan 471009; 3 Department of Cardiovascular Medicine, The First Affiliated Hospital of Henan University of Science and Technology, Luoyang, Henan 471003, P.R. China
2
Received March 5, 2014; Accepted August 15, 2014 DOI: 10.3892/etm.2014.1991 Abstract. The aim of the present study was to investigate the effect of Avastin on the number and structure of tumor blood vessels of nude mice with A549 lung adenocarcinoma. A total of 30 nude mice were randomly divided into three groups, namely the control, the Avastin I (Avastin 3 mg̸kg) and the Avastin II (Avastin 6 mg/kg) groups. Following treatment, ELISA was used to detect the expression level of vascular endothelial growth factor (VEGF) in tumor tissues. The microvascular density in tumor tissues and tumor vascular pericyte coverage was detected by immunofluorescence. The tumor growth and survival rate of mice in the three groups were also analyzed. Compared with the control group, the Avastin I and II groups exhibited significantly decreased VEGF levels and microvascular density in the tumor tissues, with the decrease in the Avastin II group being more prominent (P
