Short Communieation
Pento, J. T., S.M. GUck, A. Kagan, P.c. Gorfein: The relative influence of calcium, strontium and magnesium on calcitonin secretion in the pig. Endocrinology 94: 1176-1180 (1974) Queener, S., N.H. Bell. The effects of calcium and dibutyryl eyclic AMP on calcitonin synthesis and release in vitro. Clin.Res. 21: 848 (1973) Radde, I.C., D.K. Parkinson, E.R. Witterman, B. Hoffken: Magnesium and calcium as stimuli to in vitro release of caleitonin from thyroid slices. In: Taylor, S., Ed.: Calcitonin 1969. Wm. Heinemann Medical Books. Ltd., Landon 376-380 (1969) Roos, BA .. R. Bailey, J. Hardison, L. Bundy, L.J. Deftos: Seeretion of calcitonin in vitro. Clin.Res. 22: 348A (1974 ) Roos, B.A., K. Okano, L.J. Deftos: Biosynthesis and seeretion of calcitonin (CT) in vitro. Program of the 56th
437
Annual Meeting of the Endocrine SJciety, June 12-14: A-206 (1974) Silva, O.L., R.H. Snider, K.L. Beeker: Radioimmunoassay of human calcitonin. Clin.Chem. 20: 337-339 (1974) Sizemore, G. W., V.L. W. Go, E.L. Kaplan, L.J. Sanzenbacher, K.H. Holtermuller, C.D. Amaud: Relations of calcitonin and gastrin in Zollinger-Ellison syndrome and medullary carcinoma of the thyroid. N.Engl.J.Med. 288: 641-644 (1973) Sorgente, N., M. L 'Heureux: In vitro biosynthesis of calcitonin. Proc.Soe.Exp.BioI.Med. 136: 80-84 (1971) Swaminathan, R., R.F.L. Bates, S.R. B/oom, P.c. Ganguli, A.D. Care: The relationship between food, gastrointestinal hormones and calcitonin secretion. J.Endoerinology 59: 217-230 (1973) West, T.E. T., J.L.H. O'Riordan, D.H. Copp, R.F.L. Bates, A.D. Care: The effeet of hypocalcemia on the seeretion of calcitonin. J.Endocrinology 56: 436-470 (1973)
Short Communications
Horm. Metab. Res. 7 (1975) 437-438
© Georg Thieme Verlag Stuttgart Effect of Aminophylline on Growth Hormone and Insulin Responses to Arginine in Normal Subjects M. Peracchi, F. Cavagnini, M. Pinto, p, Bulgheroni and A.E. Panerai First Medieal Clinie. University of Milan, Milan. Italy
The stimulatory role of eyclic AMP (cAMP) on growth hormone (GH) release has been clearly demonstrated in vitro (Peake, Steinerand Daughaday 1973). However, conflicting results have been obtained in man. While cAMP infusion increases plasma GH levels (Levine, Oyama, Kagan and Glick 1970), peripherally administered aminophylline, whieh inereases the intraeellular cAMP levels by inhibiting phosphodiesterase, does not modify the basal GH levels (Ensinck, StoII, Gale, Santen, Touber and Williams 1970) and significantly decreases the GH response to hypoglycaemia in normal subjects (Pe· racchi, Cavagnini, Pontiroli, Raggi, Malinverni and Pinto 1974). The notion that arginine induees GH release by mechanisms different from those involved in hypoglycemia (Strauch, Modig/iani and Bricaire 1969, Cavagnini and Peracchi 1971) prompted us to investigate the possible effeet of aminophylline on GH response to arginine in normal subjeets. In addition, since aminophylline pretreatment has been found to inerease the insulin (IR!) response to arginine (Efendic, Cerasi and Luft 1972, Mareo, Diaz Fierros, Baroja, Villanueva and Va/verde 1972), the effeet of a simultaneous aminophylline-arginine infusion on plasma IRI levels was also studied. Materials and Methods Ten normal females, aged 21-43 yr, volunteered for this study. In eaeh subject two arginine tests (0.5 gm L-Argininejkg b.w. infused over 30 min) were performed in a random order; in one test aminophylline was injeeted in the contralateral vein during the arginine infusion, at the total dose of 480 eng, 240 mg being administered from 0 to 10 min and the remaining 240 eng during the following 20 min. Blood sampies were taken at 30 min interval for 2 hr. Blood glucose was estimated by a glucose-oxidase method. Plasma free fatty acids (FFA) were measured according to Dole and Meinertz (1960). Plasma GH and IRI were determined by a double antibody radioimmunoassay. Statistical analysis of the da ta was performed by paired Student's t-test. Results Aminophylline infusion failed to modify the GH response to arginine; the mean peak GH value being 30.7 ± 3.4 (SE) vs 27.2 ± 3.1 ngjml. By contrast, the IRI response to arginine was significantly potentiated by aminophylline; the mean IRI peak value increased from 62.1 ± 5.3 to 91.4 ± 6.5 J.IUjml (p
Pento, J. T., S.M. GUck, A. Kagan, P.c. Gorfein: The relative influence of calcium, strontium and magnesium on calcitonin secretion in the pig. Endocrinology 94: 1176-1180 (1974) Queener, S., N.H. Bell. The effects of calcium and dibutyryl eyclic AMP on calcitonin synthesis and release in vitro. Clin.Res. 21: 848 (1973) Radde, I.C., D.K. Parkinson, E.R. Witterman, B. Hoffken: Magnesium and calcium as stimuli to in vitro release of caleitonin from thyroid slices. In: Taylor, S., Ed.: Calcitonin 1969. Wm. Heinemann Medical Books. Ltd., Landon 376-380 (1969) Roos, BA .. R. Bailey, J. Hardison, L. Bundy, L.J. Deftos: Seeretion of calcitonin in vitro. Clin.Res. 22: 348A (1974 ) Roos, B.A., K. Okano, L.J. Deftos: Biosynthesis and seeretion of calcitonin (CT) in vitro. Program of the 56th
437
Annual Meeting of the Endocrine SJciety, June 12-14: A-206 (1974) Silva, O.L., R.H. Snider, K.L. Beeker: Radioimmunoassay of human calcitonin. Clin.Chem. 20: 337-339 (1974) Sizemore, G. W., V.L. W. Go, E.L. Kaplan, L.J. Sanzenbacher, K.H. Holtermuller, C.D. Amaud: Relations of calcitonin and gastrin in Zollinger-Ellison syndrome and medullary carcinoma of the thyroid. N.Engl.J.Med. 288: 641-644 (1973) Sorgente, N., M. L 'Heureux: In vitro biosynthesis of calcitonin. Proc.Soe.Exp.BioI.Med. 136: 80-84 (1971) Swaminathan, R., R.F.L. Bates, S.R. B/oom, P.c. Ganguli, A.D. Care: The relationship between food, gastrointestinal hormones and calcitonin secretion. J.Endoerinology 59: 217-230 (1973) West, T.E. T., J.L.H. O'Riordan, D.H. Copp, R.F.L. Bates, A.D. Care: The effeet of hypocalcemia on the seeretion of calcitonin. J.Endocrinology 56: 436-470 (1973)
Short Communications
Horm. Metab. Res. 7 (1975) 437-438
© Georg Thieme Verlag Stuttgart Effect of Aminophylline on Growth Hormone and Insulin Responses to Arginine in Normal Subjects M. Peracchi, F. Cavagnini, M. Pinto, p, Bulgheroni and A.E. Panerai First Medieal Clinie. University of Milan, Milan. Italy
The stimulatory role of eyclic AMP (cAMP) on growth hormone (GH) release has been clearly demonstrated in vitro (Peake, Steinerand Daughaday 1973). However, conflicting results have been obtained in man. While cAMP infusion increases plasma GH levels (Levine, Oyama, Kagan and Glick 1970), peripherally administered aminophylline, whieh inereases the intraeellular cAMP levels by inhibiting phosphodiesterase, does not modify the basal GH levels (Ensinck, StoII, Gale, Santen, Touber and Williams 1970) and significantly decreases the GH response to hypoglycaemia in normal subjects (Pe· racchi, Cavagnini, Pontiroli, Raggi, Malinverni and Pinto 1974). The notion that arginine induees GH release by mechanisms different from those involved in hypoglycemia (Strauch, Modig/iani and Bricaire 1969, Cavagnini and Peracchi 1971) prompted us to investigate the possible effeet of aminophylline on GH response to arginine in normal subjeets. In addition, since aminophylline pretreatment has been found to inerease the insulin (IR!) response to arginine (Efendic, Cerasi and Luft 1972, Mareo, Diaz Fierros, Baroja, Villanueva and Va/verde 1972), the effeet of a simultaneous aminophylline-arginine infusion on plasma IRI levels was also studied. Materials and Methods Ten normal females, aged 21-43 yr, volunteered for this study. In eaeh subject two arginine tests (0.5 gm L-Argininejkg b.w. infused over 30 min) were performed in a random order; in one test aminophylline was injeeted in the contralateral vein during the arginine infusion, at the total dose of 480 eng, 240 mg being administered from 0 to 10 min and the remaining 240 eng during the following 20 min. Blood sampies were taken at 30 min interval for 2 hr. Blood glucose was estimated by a glucose-oxidase method. Plasma free fatty acids (FFA) were measured according to Dole and Meinertz (1960). Plasma GH and IRI were determined by a double antibody radioimmunoassay. Statistical analysis of the da ta was performed by paired Student's t-test. Results Aminophylline infusion failed to modify the GH response to arginine; the mean peak GH value being 30.7 ± 3.4 (SE) vs 27.2 ± 3.1 ngjml. By contrast, the IRI response to arginine was significantly potentiated by aminophylline; the mean IRI peak value increased from 62.1 ± 5.3 to 91.4 ± 6.5 J.IUjml (p
