Effect of Acute Ethanol Ingestion on Fat Absorption 1 MICHELINE BOQUILLON, E.R., 188 C.N.R.S., U.E.R., Nutrition, Sciences Mirande, Campus Universitaire, 21 000 Dijon, France
transported by small particles moving in the region of a , globulins in cellulose acetate electrophoresis than by larger particles remaining at the origin. This proportion was enhanced in the ethanoltreated animals. The larger fat particles were richer in endogenous fatty acids in alcohol-treated rats than in controls.
ABSTRACT
A test meal (300 mg casein, 600 mg sucrose, 100 mg corn oil, tracer dose of 9.103H oleic acid) was given to fasting adult rats with intestinal lymph fistulas. One group received an acute oral dose of ethanol (3.2 g/kg body weight) simultaneously with the test meal. Controls received 2.5 ml of water instead of ethanol. Ingestion of ethanol temporarily delayed the removal of lipid radioactivity from the stomachs. More than 25% of radioactivity fed remained 8 hr after feeding whereas with control rats less than 10% of lipid radioactivity fed remained 6 hr after feeding. In controls and ethanoltreated rats, the amounts of exogenous lipids in the intestinal lumen and mucosa were low and similar enough. Quantities of endogenous and exogenous lipids found in the lymph collected during 24 hr after feeding were similar in the two groups, but the fat absorption peak was found after 6 hr in alcoholic rats and before 6 hr in controls. This delay was probably due to the retention of tipids in the stomach. More of the exogenous lipid was always
INTRODUCTION
This study was undertaken to investigate the effects of acute ethanol administration on the lymph lipid output and the lymph fat particle size after lipid feeding. Previously (1,2) we have shown that cellulose acetate electrophoresis of mesenteric lymph obtained from rats fed a diet containing 14C or 3H fatty acids exhibited two labeled bands. The first (M0) remained at the origin whereas the second ( M a l ) moved in the region of al-globulins (see Fig. 1). Since these two kinds of lipoproteins can also be separated by density gradient zonal centrifugation (2) and investigated by electron microscopy (2,3), we have observed that after feeding corn oil, the particles of band M0 have a larger diameter (0.1 /am~
transported by small particles moving in the region of a , globulins in cellulose acetate electrophoresis than by larger particles remaining at the origin. This proportion was enhanced in the ethanoltreated animals. The larger fat particles were richer in endogenous fatty acids in alcohol-treated rats than in controls.
ABSTRACT
A test meal (300 mg casein, 600 mg sucrose, 100 mg corn oil, tracer dose of 9.103H oleic acid) was given to fasting adult rats with intestinal lymph fistulas. One group received an acute oral dose of ethanol (3.2 g/kg body weight) simultaneously with the test meal. Controls received 2.5 ml of water instead of ethanol. Ingestion of ethanol temporarily delayed the removal of lipid radioactivity from the stomachs. More than 25% of radioactivity fed remained 8 hr after feeding whereas with control rats less than 10% of lipid radioactivity fed remained 6 hr after feeding. In controls and ethanoltreated rats, the amounts of exogenous lipids in the intestinal lumen and mucosa were low and similar enough. Quantities of endogenous and exogenous lipids found in the lymph collected during 24 hr after feeding were similar in the two groups, but the fat absorption peak was found after 6 hr in alcoholic rats and before 6 hr in controls. This delay was probably due to the retention of tipids in the stomach. More of the exogenous lipid was always
INTRODUCTION
This study was undertaken to investigate the effects of acute ethanol administration on the lymph lipid output and the lymph fat particle size after lipid feeding. Previously (1,2) we have shown that cellulose acetate electrophoresis of mesenteric lymph obtained from rats fed a diet containing 14C or 3H fatty acids exhibited two labeled bands. The first (M0) remained at the origin whereas the second ( M a l ) moved in the region of al-globulins (see Fig. 1). Since these two kinds of lipoproteins can also be separated by density gradient zonal centrifugation (2) and investigated by electron microscopy (2,3), we have observed that after feeding corn oil, the particles of band M0 have a larger diameter (0.1 /am~
