151 Horm. Metab. Res. 8 (1976)151-152
© Georg Thieme Verlag Stuttgart Effect of Acute Changes in Serum Calcium on Glucose Tolerance, Blood Glucose, IRI and Cortisol Levels L. Gerö, F. Szalay, K. Steczek and Gy. Tamas Jr. First Department of Medicine, Semmelweis University, Budapest, Hungary
Introduction According to experimental data calcium ions are involved both in the secretory process (Grodsky and Bennett 1966, Mi/ner and Haies 1967, Malaisse and Malaisse-Lagae 1970)_and the action of imulin (Kissebah, Hope-Gill, Vydelingum, Tulloch, Garke and Fraser 1975). Since only a few cJinicai observations have been reported on this subject so far, it seemed worthwhile to investigate the effect of an altered serum calcium on glucose tolerance, blood glucose and IRI levels. 11 diabetics - treated with diet or diet + antidiabetic drugs and 53 non-diabetics were investigated: all the diabetics and 46 of the non-diabetics (normal group) received 10%, caa 2 (3.6 mg Ca++/kg b.w.) intravenously. Blood was taken before and 5,15,30,60, 120 and 180 minutes after the i.v. injection for the determination of serum calcium (Ray Sarkar and Chau· han 1967), glucose (o-toluidin method), IRI (Amersham-KIT, double antibody technique of Haies and Randle 1963), and cortisol (fluorimetric method of Mattingly 1962), 7 of the non-diabetics served as controls: their blood glucose and cortisol were measured without previous calcium load. A self-controlled mathematical analysis was carried out using Student's t test. AU the changes were compared to the initial (zero minute) values and the means were given within the 95% fiducial limits: i( ± t(0.5) . si(' In a further study 50 g oGTT was performed in five hypocalcemic patients (see Table). Three days later the oGTT was repeated with a simultaneous i.v. caa 2 injection (3.6 mg Ca++/kg b.w.). Blood sugar and IRI were determined from venous blood sampies at 0, 15, 30, 60 and 120 minutes.
OIAEETIC
GROJP
N(l!MAL
GROUP
11
10 9~~--~-.--~~
IRI
!-E/rn
Fig. 1. Changes in blood glucose, IRI and cortisol foUowing i.v. calcium load. AU the changes were compared to the initial values, the means are given within the 95% fiducial limits. • denotes significance.
Rec.: 28 AUt!. 1975
Ace.: 16 Nov. 1975
Downloaded by: University of British Columbia. Copyrighted material.
Materials and Methods
Short Communications
152
B.
A 800d 1W glucose
~
l~~-StA
..
o or-r---r7530
i
60
l~ o
75 iJ 6'0. 720 mtxJtes
Table Name
Age Sex (years)
K.A. K.M.
70 47
M F
H.E.
20 32 51
F M F
Sz.A. V.A.
Diagnosis
osteoporosis post-thyreoidectomy tetany
..
chr.renal failure osteomalacia
Fasting serum calcium (mg/ 100 ml) 7.8 8.5 7.2 7.9 6.6
Fig. 2. Time-courses of blood glucose and IRI during 50 g oGTT, without (A) and with (B) a simultaneous i.v. calcium load.
Results 1. a. In the diabetic group blood glucose levels decreased significantly at 60, 120 and 180 minutes (P 0.05, P 0.05 and P 0.01 resp.), however, no significant change in IRI occurred. Plasma cortisol values rose significantly at 5, 15, 30 and 60 minutes (P 0.01 for all).
© Georg Thieme Verlag Stuttgart Effect of Acute Changes in Serum Calcium on Glucose Tolerance, Blood Glucose, IRI and Cortisol Levels L. Gerö, F. Szalay, K. Steczek and Gy. Tamas Jr. First Department of Medicine, Semmelweis University, Budapest, Hungary
Introduction According to experimental data calcium ions are involved both in the secretory process (Grodsky and Bennett 1966, Mi/ner and Haies 1967, Malaisse and Malaisse-Lagae 1970)_and the action of imulin (Kissebah, Hope-Gill, Vydelingum, Tulloch, Garke and Fraser 1975). Since only a few cJinicai observations have been reported on this subject so far, it seemed worthwhile to investigate the effect of an altered serum calcium on glucose tolerance, blood glucose and IRI levels. 11 diabetics - treated with diet or diet + antidiabetic drugs and 53 non-diabetics were investigated: all the diabetics and 46 of the non-diabetics (normal group) received 10%, caa 2 (3.6 mg Ca++/kg b.w.) intravenously. Blood was taken before and 5,15,30,60, 120 and 180 minutes after the i.v. injection for the determination of serum calcium (Ray Sarkar and Chau· han 1967), glucose (o-toluidin method), IRI (Amersham-KIT, double antibody technique of Haies and Randle 1963), and cortisol (fluorimetric method of Mattingly 1962), 7 of the non-diabetics served as controls: their blood glucose and cortisol were measured without previous calcium load. A self-controlled mathematical analysis was carried out using Student's t test. AU the changes were compared to the initial (zero minute) values and the means were given within the 95% fiducial limits: i( ± t(0.5) . si(' In a further study 50 g oGTT was performed in five hypocalcemic patients (see Table). Three days later the oGTT was repeated with a simultaneous i.v. caa 2 injection (3.6 mg Ca++/kg b.w.). Blood sugar and IRI were determined from venous blood sampies at 0, 15, 30, 60 and 120 minutes.
OIAEETIC
GROJP
N(l!MAL
GROUP
11
10 9~~--~-.--~~
IRI
!-E/rn
Fig. 1. Changes in blood glucose, IRI and cortisol foUowing i.v. calcium load. AU the changes were compared to the initial values, the means are given within the 95% fiducial limits. • denotes significance.
Rec.: 28 AUt!. 1975
Ace.: 16 Nov. 1975
Downloaded by: University of British Columbia. Copyrighted material.
Materials and Methods
Short Communications
152
B.
A 800d 1W glucose
~
l~~-StA
..
o or-r---r7530
i
60
l~ o
75 iJ 6'0. 720 mtxJtes
Table Name
Age Sex (years)
K.A. K.M.
70 47
M F
H.E.
20 32 51
F M F
Sz.A. V.A.
Diagnosis
osteoporosis post-thyreoidectomy tetany
..
chr.renal failure osteomalacia
Fasting serum calcium (mg/ 100 ml) 7.8 8.5 7.2 7.9 6.6
Fig. 2. Time-courses of blood glucose and IRI during 50 g oGTT, without (A) and with (B) a simultaneous i.v. calcium load.
Results 1. a. In the diabetic group blood glucose levels decreased significantly at 60, 120 and 180 minutes (P 0.05, P 0.05 and P 0.01 resp.), however, no significant change in IRI occurred. Plasma cortisol values rose significantly at 5, 15, 30 and 60 minutes (P 0.01 for all).
