This article was downloaded by: [Tufts University] On: 14 October 2014, At: 11:10 Publisher: Taylor & Francis Informa Ltd Registered in England and Wales Registered Number: 1072954 Registered office: Mortimer House, 37-41 Mortimer Street, London W1T 3JH, UK
New Zealand Veterinary Journal Publication details, including instructions for authors and subscription information: http://www.tandfonline.com/loi/tnzv20
Echinococcus granulosus, Taenia hydatigena, T. ovis: Evaluation of cyst fluids as antigen for serodiagnosis of larval cestodes in sheep a
a
W.K. Yong , D.D. Heath & S.N. Parmeter
a
a
Research Division, Ministry of Agriculture and Fisheries , Wallaceville Animal Research Centre , Private Bag, Upper Hutt, New Zealand Published online: 23 Feb 2011.
To cite this article: W.K. Yong , D.D. Heath & S.N. Parmeter (1978) Echinococcus granulosus, Taenia hydatigena, T. ovis: Evaluation of cyst fluids as antigen for serodiagnosis of larval cestodes in sheep, New Zealand Veterinary Journal, 26:9, 231-234, DOI: 10.1080/00480169.1978.34550 To link to this article: http://dx.doi.org/10.1080/00480169.1978.34550
PLEASE SCROLL DOWN FOR ARTICLE Taylor & Francis makes every effort to ensure the accuracy of all the information (the “Content”) contained in the publications on our platform. However, Taylor & Francis, our agents, and our licensors make no representations or warranties whatsoever as to the accuracy, completeness, or suitability for any purpose of the Content. Any opinions and views expressed in this publication are the opinions and views of the authors, and are not the views of or endorsed by Taylor & Francis. The accuracy of the Content should not be relied upon and should be independently verified with primary sources of information. Taylor and Francis shall not be liable for any losses, actions, claims, proceedings, demands, costs, expenses, damages, and other liabilities whatsoever or howsoever caused arising directly or indirectly in connection with, in relation to or arising out of the use of the Content. This article may be used for research, teaching, and private study purposes. Any substantial or systematic reproduction, redistribution, reselling, loan, sub-licensing, systematic supply, or distribution in any form to anyone is expressly forbidden. Terms & Conditions of access and use can be found at http:// www.tandfonline.com/page/terms-and-conditions
1978
NEW ZEALAND VETERINARY JOURNAL
231
Echinococcus granulosus, Taenia hydatigena, T. ovis: Evaluat.ion of cyst fluids as antigen for serodiagnosis of larval cestodes In sheep w. K. Yong*, D. D. Heath*, s. N. Parmeter* N.Z.vel.J.26: 231-4
Downloaded by [Tufts University] at 11:10 14 October 2014
ABSTRACT
In order to monitor the progress of New Zealand's hydatids eradication campaign, a specific, serological, diagnostic test is required to identify infected sheep. An indirect haemagglutination test, using pyruvic aldehyde-stabilized sheep erythrocytes as the antigen carrier, was developed for the serodiagnosis oflarval cestode infections of sheep. Using cyst fluid from Echinococcus granulosus, Taenia hydatigena and T. ovis as the antigens in this test, it was shown that the larval cestode species, responsible for an infection in sheep harbouring a single specific infection, could be identified by the higher titre given with the homologous antigen, in comparison to that given with the heterologous antigens. Sera of sheep infected with two or more species were also tested by this method, and the only specific infections to be diagnosed by differential titres were those due to the presence of live E. granulosus cysts. These antigens cannot be used for the diagnosis of specific larval cestode infections in the field because of the cross-reactivity between cyst fluids. However, the test did show that infection with larval cestodes could be diagnosed on a non-specific basis.
INTRODUCTION
In New Zealand, three larval cestode species are known to be present in sheep. These are Echinococcus granulosus, Cysticercus tenuicollis and C. ovis, all of which share the same sheep-dogsheep life cycle. Studies on the serology of larval cestode infections in sheep are few. Lamy et al.(II) studied complement fixation tests, whilst others(3)(19) used an indirect haemagglutination (IRA) test. The latter appeared to give more consistent results in identifying existing infections in animals than did the former; probably because complement fixation tests have been shown to monitor infections with dead cysts better than infections with live cysts(7). These observations agreed with findings from studies on serodiagnosis for human hydatidosis(IO)(21). Lamy et al.(II) and Blundell-Rasell
New Zealand Veterinary Journal Publication details, including instructions for authors and subscription information: http://www.tandfonline.com/loi/tnzv20
Echinococcus granulosus, Taenia hydatigena, T. ovis: Evaluation of cyst fluids as antigen for serodiagnosis of larval cestodes in sheep a
a
W.K. Yong , D.D. Heath & S.N. Parmeter
a
a
Research Division, Ministry of Agriculture and Fisheries , Wallaceville Animal Research Centre , Private Bag, Upper Hutt, New Zealand Published online: 23 Feb 2011.
To cite this article: W.K. Yong , D.D. Heath & S.N. Parmeter (1978) Echinococcus granulosus, Taenia hydatigena, T. ovis: Evaluation of cyst fluids as antigen for serodiagnosis of larval cestodes in sheep, New Zealand Veterinary Journal, 26:9, 231-234, DOI: 10.1080/00480169.1978.34550 To link to this article: http://dx.doi.org/10.1080/00480169.1978.34550
PLEASE SCROLL DOWN FOR ARTICLE Taylor & Francis makes every effort to ensure the accuracy of all the information (the “Content”) contained in the publications on our platform. However, Taylor & Francis, our agents, and our licensors make no representations or warranties whatsoever as to the accuracy, completeness, or suitability for any purpose of the Content. Any opinions and views expressed in this publication are the opinions and views of the authors, and are not the views of or endorsed by Taylor & Francis. The accuracy of the Content should not be relied upon and should be independently verified with primary sources of information. Taylor and Francis shall not be liable for any losses, actions, claims, proceedings, demands, costs, expenses, damages, and other liabilities whatsoever or howsoever caused arising directly or indirectly in connection with, in relation to or arising out of the use of the Content. This article may be used for research, teaching, and private study purposes. Any substantial or systematic reproduction, redistribution, reselling, loan, sub-licensing, systematic supply, or distribution in any form to anyone is expressly forbidden. Terms & Conditions of access and use can be found at http:// www.tandfonline.com/page/terms-and-conditions
1978
NEW ZEALAND VETERINARY JOURNAL
231
Echinococcus granulosus, Taenia hydatigena, T. ovis: Evaluat.ion of cyst fluids as antigen for serodiagnosis of larval cestodes In sheep w. K. Yong*, D. D. Heath*, s. N. Parmeter* N.Z.vel.J.26: 231-4
Downloaded by [Tufts University] at 11:10 14 October 2014
ABSTRACT
In order to monitor the progress of New Zealand's hydatids eradication campaign, a specific, serological, diagnostic test is required to identify infected sheep. An indirect haemagglutination test, using pyruvic aldehyde-stabilized sheep erythrocytes as the antigen carrier, was developed for the serodiagnosis oflarval cestode infections of sheep. Using cyst fluid from Echinococcus granulosus, Taenia hydatigena and T. ovis as the antigens in this test, it was shown that the larval cestode species, responsible for an infection in sheep harbouring a single specific infection, could be identified by the higher titre given with the homologous antigen, in comparison to that given with the heterologous antigens. Sera of sheep infected with two or more species were also tested by this method, and the only specific infections to be diagnosed by differential titres were those due to the presence of live E. granulosus cysts. These antigens cannot be used for the diagnosis of specific larval cestode infections in the field because of the cross-reactivity between cyst fluids. However, the test did show that infection with larval cestodes could be diagnosed on a non-specific basis.
INTRODUCTION
In New Zealand, three larval cestode species are known to be present in sheep. These are Echinococcus granulosus, Cysticercus tenuicollis and C. ovis, all of which share the same sheep-dogsheep life cycle. Studies on the serology of larval cestode infections in sheep are few. Lamy et al.(II) studied complement fixation tests, whilst others(3)(19) used an indirect haemagglutination (IRA) test. The latter appeared to give more consistent results in identifying existing infections in animals than did the former; probably because complement fixation tests have been shown to monitor infections with dead cysts better than infections with live cysts(7). These observations agreed with findings from studies on serodiagnosis for human hydatidosis(IO)(21). Lamy et al.(II) and Blundell-Rasell
