HHS Public Access Author manuscript Author Manuscript
Am J Perinatol. Author manuscript; available in PMC 2017 February 13. Published in final edited form as: Am J Perinatol. 2017 February ; 34(3): 240–247. doi:10.1055/s-0036-1585419.
Early Elevation in Interleukin-6 is Associated with Reduced Growth in Extremely Low Birth Weight Infants Lee A. Denson, MD1, Scott A. McDonald, BS2, Abhik Das, PhD2, Diana E. Schendel, PhD3, Kristin Skogstrand, PhD4, David M. Hougaard, MD DSc4, Seetha Shankaran, MD5, Rosemary D. Higgins, MD6, Waldemar A. Carlo, MD7, and Richard A. Ehrenkranz, MD8 for the Cytokines Study Subcommittee of the NICHD Neonatal Research Network
Author Manuscript
1Gastroenterology,
Hepatology, and Nutrition, Department of Pediatrics, Cincinnati Children’s Hospital Medical Center and the University of Cincinnati College of Medicine, Cincinnati, OH 2RTI
International, Research Triangle Park, NC
3Centers
for Disease Control and Prevention, Atlanta, GA
4Section
of Neonatal Screening and Hormones, Department of Clinical Biochemistry and Immunology, Statens Serum Institut, Copenhagen, Denmark
5Neonatal-Perinatal
Medicine, Department of Pediatrics, Wayne State University, Detroit, MI
6Eunice
Kennedy Shriver National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD
Author Manuscript
7Division
of Neonatology, Department of Pediatrics, University of Alabama at Birmingham, Birmingham, AL 8Division
of Perinatal Medicine, Department of Pediatrics, Yale-New Haven Children’s Hospital and the Yale University School of Medicine, New Haven, CT
Abstract Objective—To determine whether reduced growth velocity (GV) in extremely low birth weight (ELBW) infants is preceded by elevated inflammatory cytokines. Study Design—GV was determined at 36 weeks post-menstrual age (PMA) in 768 infants 401-1000 g birth weight (BW). Association between blood cytokines measured through day of life
Author Manuscript
Corresponding Author: Lee A. Denson, MLC 2010, 3333 Burnet Avenue, Cincinnati, OH 45229, Tel: 513-636-7575: Fax: 513-636-5581: [email protected]. Author roles: Study concept and design: LD, RE Acquisition of Data: DS, PT, KS, DH, SS, WC, RE Analysis and Interpretation: LD, SM, AD, RE Drafting of Manuscript: LD, SM, AD, RE Critical revision of manuscript: LD, SM, AD, DS, SS, RH, WC, RE Statistical analysis: SM, AD Obtained funding: LD, RE Technical/Material Support: DS, PT, KS, DH Study Supervision: LD, RE
Denson et al.
Page 2
Author Manuscript
21 and GV was explored using linear regression models that adjusted for late-onset sepsis (LOS), BW, small-for-gestational age (SGA), gender, race, energy intake, and center. Results—Serum interleukin-6 (IL-6) was increased at days 14 and 21 in LOS infants. LOS was associated with reduced energy intake and GV for weight (weight-GV) at 36 weeks PMA. Linear regression analysis controlling for LOS and energy intake showed significant relationships between increased IL-6 at days 14 and 21 with reduced weight-GV at 36 weeks PMA (p
Am J Perinatol. Author manuscript; available in PMC 2017 February 13. Published in final edited form as: Am J Perinatol. 2017 February ; 34(3): 240–247. doi:10.1055/s-0036-1585419.
Early Elevation in Interleukin-6 is Associated with Reduced Growth in Extremely Low Birth Weight Infants Lee A. Denson, MD1, Scott A. McDonald, BS2, Abhik Das, PhD2, Diana E. Schendel, PhD3, Kristin Skogstrand, PhD4, David M. Hougaard, MD DSc4, Seetha Shankaran, MD5, Rosemary D. Higgins, MD6, Waldemar A. Carlo, MD7, and Richard A. Ehrenkranz, MD8 for the Cytokines Study Subcommittee of the NICHD Neonatal Research Network
Author Manuscript
1Gastroenterology,
Hepatology, and Nutrition, Department of Pediatrics, Cincinnati Children’s Hospital Medical Center and the University of Cincinnati College of Medicine, Cincinnati, OH 2RTI
International, Research Triangle Park, NC
3Centers
for Disease Control and Prevention, Atlanta, GA
4Section
of Neonatal Screening and Hormones, Department of Clinical Biochemistry and Immunology, Statens Serum Institut, Copenhagen, Denmark
5Neonatal-Perinatal
Medicine, Department of Pediatrics, Wayne State University, Detroit, MI
6Eunice
Kennedy Shriver National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD
Author Manuscript
7Division
of Neonatology, Department of Pediatrics, University of Alabama at Birmingham, Birmingham, AL 8Division
of Perinatal Medicine, Department of Pediatrics, Yale-New Haven Children’s Hospital and the Yale University School of Medicine, New Haven, CT
Abstract Objective—To determine whether reduced growth velocity (GV) in extremely low birth weight (ELBW) infants is preceded by elevated inflammatory cytokines. Study Design—GV was determined at 36 weeks post-menstrual age (PMA) in 768 infants 401-1000 g birth weight (BW). Association between blood cytokines measured through day of life
Author Manuscript
Corresponding Author: Lee A. Denson, MLC 2010, 3333 Burnet Avenue, Cincinnati, OH 45229, Tel: 513-636-7575: Fax: 513-636-5581: [email protected]. Author roles: Study concept and design: LD, RE Acquisition of Data: DS, PT, KS, DH, SS, WC, RE Analysis and Interpretation: LD, SM, AD, RE Drafting of Manuscript: LD, SM, AD, RE Critical revision of manuscript: LD, SM, AD, DS, SS, RH, WC, RE Statistical analysis: SM, AD Obtained funding: LD, RE Technical/Material Support: DS, PT, KS, DH Study Supervision: LD, RE
Denson et al.
Page 2
Author Manuscript
21 and GV was explored using linear regression models that adjusted for late-onset sepsis (LOS), BW, small-for-gestational age (SGA), gender, race, energy intake, and center. Results—Serum interleukin-6 (IL-6) was increased at days 14 and 21 in LOS infants. LOS was associated with reduced energy intake and GV for weight (weight-GV) at 36 weeks PMA. Linear regression analysis controlling for LOS and energy intake showed significant relationships between increased IL-6 at days 14 and 21 with reduced weight-GV at 36 weeks PMA (p
