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Draft Genome Sequence of Lactobacillus gorillae Strain KZ01T, Isolated from a Western Lowland Gorilla Sayaka Tsuchida,a Maiko Nezuo,b Masatoshi Tsukahara,b Yoshitoshi Ogura,c Tetsuya Hayashi,c Kazunari Ushidaa Graduate School of Life and Environmental Sciences, Kyoto Prefectural University, Kyoto-shi, Kyoto, Japana; BioJet Co., Ltd, Uruma-shi, Okinawa, Japanb; Department of Bacteriology, Faculty of Medical Sciences, Kyushu University, Fukuoka-shi, Fukuoka, Japanc

Here, we report the draft genome sequence of Lactobacillus gorillae strain KZ01T isolated from a western lowland gorilla (Gorilla gorilla gorilla). This genome sequence will be helpful for the comparative genomics between human and nonhuman primate-associated Lactobacillus. Received 30 August 2015 Accepted 3 September 2015 Published 15 October 2015 Citation Tsuchida S, Nezuo M, Tsukahara M, Ogura Y, Hayashi T, Ushida K. 2015. Draft genome sequence of Lactobacillus gorillae strain KZ01T, isolated from a western lowland gorilla. Genome Announc 3(5):e01196-15. doi:10.1128/genomeA.01196-15. Copyright © 2015 Tsuchida et al. This is an open-access article distributed under the terms of the Creative Commons Attribution 3.0 Unported license. Address correspondence to Kazunari Ushida, [email protected]

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actobacillus gorillae bacteria were isolated from Gabonese wild and Japanese captive western lowland gorillas (1). The 16S rRNA sequence of this species was detected in the feces of wild Ugandan eastern gorillas too. L. gorillae is phylogenetically related to human-associated L. fermentum, but L. gorillae has not been found in humans so far. Therefore, this species can be considered as gorilla-specific or nonhuman primate specific Lactobacillus. Thus, the genome information of L. gorillae will help identify the factors involved in the adaptation of lactobacilli to the intestinal environment of the Homininae. The genomic DNA of strain KZ01T (⫽JCM19575T) was extracted and purified from the cells grown on a de Man, Rogosa, Sharpe (MRS) agar plate (Difco) by Quickgene Mini 80 (Kurabo, Tokyo Japan). Illumina Miseq produced 1,183,050 pair-ended reads representing an average coverage of 118-fold. The reads were assembled by CLC Genomics Workbench 8.0.1 after trimming low-quality sequences. The final draft genome consists of 63 scaffolds (from 1.1 to 106 kb in length). The total length of the genome assembly is 1,641,621 bp, with an N50 equal to 44 kbp. Gene identification and annotation was performed using the Microbial Genome Annotation Pipeline online server, and additional database searches for RefSeq, TrEMBL, clusters of orthologous groups (COGs), and KEGG (2, 3). Genes for antibiotic resistance were estimated by ARDB (4). The genome size of L. gorillae strain KZ01 (1.64 Mb) is smaller than previously sequenced Lactobacillus species (1.8 to 3.3 Mb) (5). The G⫹C content of KZ01 (48.1%) is relatively higher among sequenced Lactobacillus species (34 to 51%) (5). The draft genome contains 53 tRNAs and 3 rRNAs (one 16S rRNA, one 23S rRNA, and one 5S rRNA) in addition to 1,582 protein coding sequences (CDSs). Among these, specific COGs were assigned to 1,439 CDSs. Genes for carbohydrate metabolism showed the highest prevalence (10.9%) followed by nucleic acid metabolism (8.4%) and amino acid metabolism (6.0%). Since antibiotic resistance of Lactobacillus has been issued for the safety of their probiotic use

September/October 2015 Volume 3 Issue 5 e01196-15

(6), we have particular interest in resistance-related genes of KZ01. KEGG and ARDB suggested the presence of genes for penicillin binding protein 1A and 2A, and the mraY, murG, and murF genes and those involved in the van operon in the KZ01 genome. In addition to these genes for the natural resistance, the presences of catB1, vatB, macB, and lmrB were suggested. The genome of L. gorillae KZ01 will provide a foundation for gastrointestinal microbiota of wild animals and fermentation and comparative genomics between human and nonhuman primate-associated lactobacilli. Nucleotide sequence accession numbers. The sequence information was submitted to DDBJ/EMBL/GenBank under the accession no. BCAH01000001 to BCAH01000063. ACKNOWLEDGMENTS The study was supported by the Ministry of Education, Culture, Sports, Science and Technology (221S0002) for Genome Science and the JSPS KAKENHI (15K18775).

REFERENCES 1. Tsuchida S, Kitahara M, Nguema PP, Norimitsu S, Fujita S, Yamagiwa J, Ngomanda A, Ohkuma M, Ushida K. 2014. Lactobacillus gorillae sp. nov., isolated from the faeces of captive and wild western lowland gorillas (Gorilla gorilla gorilla). Int J Syst Evol Microbiol 64:4001– 4006. http:// dx.doi.org/10.1099/ijs.0.068429-0. 2. Sugawara H, Ohyama A, Mori H, Kurokawa K. 2009. Microbial genome annotation pipeline (MiGAP) for diverse users, Abstr. 20th Int. Conf. Genome Informatics, Yokohama, Japan. 3. Kanehisa M, Goto S, Sato Y, Kawashima M, Furumichi M, Tanabe M. 2014. Data, information, knowledge and principle: back to metabolism in KEGG. Nucleic Acids Res 42:D199 –D205. http://dx.doi.org/10.1093/nar/ gkt1076. 4. Liu B, Pop M. 2009. ARDB—antibiotic resistance genes database. Nucleic Acids Res 37:D443–D447. http://dx.doi.org/10.1093/nar/gkn656. 5. Blom J, Palva A, Siezen R, de Vos WM. 2010. Comparative genomics of Lactobacillus. Microb Biotechnol 4:323–332. 6. Mathur S, Singh R. 2005. Antibiotic resistance in food lactic acid bacteria—a review. Int J Food Microbiol 105:281–295. http://dx.doi.org/ 10.1016/j.ijfoodmicro.2005.03.008.

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Draft Genome Sequence of Lactobacillus gorillae Strain KZ01T, Isolated from a Western Lowland Gorilla.

Here, we report the draft genome sequence of Lactobacillus gorillae strain KZ01(T) isolated from a western lowland gorilla (Gorilla gorilla gorilla). ...
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