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Draft Genome Sequence of Alcanivorax sp. Strain KX64203 Isolated from Deep-Sea Sediments of Iheya North, Okinawa Trough Huan Zhang,a Rui Liu,a Mengqiang Wang,a Hao Wang,a Qiang Gao,a Zhanhui Hou,a Dahai Gao,a Lingling Wanga,b Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences, Qingdao, Chinaa; Key Laboratory of Mariculture & Stock Enhancement in North China’s Sea, Ministry of Agriculture, Dalian Ocean University, Dalian, Chinab

This report describes the draft genome sequence of Alcanivorax sp. strain KX64203, isolated from deep-sea sediment samples. The reads generated by an Ion Torrent PGM were assembled into contigs, with a total size of 4.76 Mb. The data will improve our understanding of the strain’s function in alkane degradation. Received 27 June 2016 Accepted 29 June 2016 Published 25 August 2016 Citation Zhang H, Liu R, Wang M, Wang H, Gao Q, Hou Z, Gao D, Wang L. 2016. Draft genome sequence of Alcanivorax sp. strain KX64203 isolated from deep-sea sediments of Iheya North, Okinawa Trough. Genome Announc 4(4):e00872-16. doi:10.1128/genomeA.00872-16. Copyright © 2016 Zhang et al. This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license. Address correspondence to Huan Zhang, [email protected], or Lingling Wang, [email protected].

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acteria of the genus Alcanivorax belong to a group of slowgrowing marine hydrocarbonoclastic bacteria that can use oil hydrocarbons as its exclusive source of carbon and energy (1). By now, the genome sequences of six species in this genus isolated from various marine environments have been reported, including A. borkumensis (1), A. pacificus W11-5T (2), A. hongdengensis A-11-3T (3), A. dieselolei B5T (4), and A. jadensis. The Alcanivorax sp. strain KX64203 was isolated from sediment samples which were collected by using an electrohydraulic grab with an underwater television camera during the cruise conducted by the scientific research vessel KEXUE in Okinawa Trough (126°54.32= E, 27°48.47= N, ~1190-m depth, around a hydrothermal vent). Analysis of the 16S rRNA gene sequence (GenBank accession number KU954765) showed that it shared 99% identity (100% coverage) with that of A. dieselolei strain PTG4-3, and almost 99% identity with this gene in other Alcanivorax strains. The sequencing reads were generated by an Ion Torrent PGM using 314 Chip version 2 and a 400-bp sequencing kit, assembled by the Torrent SPAdes plugin (version 2.3) de novo assembler, and then merged using a CISA contig integrator (5). Protein-coding sequences were predicted by Glimmer software (version 3.02) (6) Ribosomal RNA genes were detected using RNAmmer software version 1.2 (7) and tRNA genes were detected using tRNAscan-SE (8). The genome of KX64203 consisted of 4,757,396 bases in 79 contigs (N50 159,927 bp and N90 36,849 bp), and had a G⫹C content of 61.4%. Both the genome size and G⫹C content were similar to published data for A. dieselolei type strain B5 (total length of 4.86 Mb and G⫹C% of 61.5%). Forty-three tRNA genes for all 20 amino acids and six rRNA genes were identified in the draft genome sequence. There were a total of 4,464 putative open reading frames (with an average size of 932 bp), giving a coding intensity of 87.43%. A total of 2,988 proteins were assigned to cluster of orthologous group (COG) families. The genes encoding three integral-membrane alkane monooxygenases (AlkB) and only one cytochrome P450 enzyme were found in the genome. Moreover, there were also some other proteins involved in the

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alkane degradation pathway, including three AlkKs, one AlkL, and one AlkN. Two genes, rubA and rubB encoding a rubredoxin and a rubredoxin reductase, respectively, were likely to be involved in alkane catabolism. The genome sequence and its gene annotation shed new light on the functional genomics of this species. Accession number(s). This whole-genome shotgun project has been deposited in DDBJ/ENA/GenBank under the accession no. LVIC00000000. The version described in this study is the first version LVIC01000000. ACKNOWLEDGMENTS We thank the research vessel KEXUE of the Chinese Academy of Sciences for collecting samples and the WPOS sample center for providing samples. We also thank Ping Zhang and Zengfang Zhao from the High Performance Computing Center of the Institute of Oceanology, CAS, for the computing resources and service they kindly provided. This work was supported by grants from the Strategic Priority Research Program of the Chinese Academy of Sciences (XDA11030202) and High Technology Project (863 Project, 2014AA093501) from the Chinese Ministry of Science and Technology.

FUNDING INFORMATION This work, including the efforts of Huan Zhang, was funded by Strategic Priority Research Program of CAS (XDA11030202). This work, including the efforts of Lingling Wang, was funded by High Technology Project (2014AA093501).

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Alcanivorax hongdengensis type strain A-11-3. J Bacteriol 194:6972. http:// dx.doi.org/10.1128/JB.01849-12. 4. Lai Q, Li W, Shao Z. 2012. Complete genome sequence of Alcanivorax dieselolei type strain B5. J Bacteriol 194:6674. http://dx.doi.org/10.1128/ JB.01813-12. 5. Lin SH, Liao YC. 2013. CISA: contig integrator for sequence assembly of bacterial genomes. PLoS One 8:e60843. http://dx.doi.org/10.1371/ journal.pone.0060843. 6. Delcher AL, Bratke KA, Powers EC, Salzberg SL. 2007. Identifying bac-

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Genome Announcements

July/August 2016 Volume 4 Issue 4 e00872-16

Draft Genome Sequence of Alcanivorax sp. Strain KX64203 Isolated from Deep-Sea Sediments of Iheya North, Okinawa Trough.

This report describes the draft genome sequence of Alcanivorax sp. strain KX64203, isolated from deep-sea sediment samples. The reads generated by an ...
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