Journal of Drug Targeting
ISSN: 1061-186X (Print) 1029-2330 (Online) Journal homepage: http://www.tandfonline.com/loi/idrt20
Serum level of microRNA-147 as diagnostic biomarker in human non-small cell lung caner Guangmin Chu, Jianbo Zhang & Xiaobing Chen To cite this article: Guangmin Chu, Jianbo Zhang & Xiaobing Chen (2015): Serum level of microRNA-147 as diagnostic biomarker in human non-small cell lung caner, Journal of Drug Targeting, DOI: 10.3109/1061186X.2015.1116539 To link to this article: http://dx.doi.org/10.3109/1061186X.2015.1116539
Accepted author version posted online: 18 Nov 2015.
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Full Terms & Conditions of access and use can be found at http://www.tandfonline.com/action/journalInformation?journalCode=idrt20 Download by: [Monash University Library]
Date: 24 November 2015, At: 02:32
Just Accepted by Journal of Drug Targeting Serum level of microRNA-147 as diagnostic biomarker in human nonsmall cell lung caner Guangmin Chu, Jianbo Zhang, Xiaobing Chen doi: 10.3109/1061186X.2015.1116539 Abstract
Downloaded by [Monash University Library] at 02:32 24 November 2015
Objectives: In this study, we intended to examine the gene expression level and the clinical significance of microRNA-147 (miR-147) in cancer tissues and sera of patients with non-small cell lung cancer (NSCLC). Methods: Quantitative real-time PCR (qRT-PCR) was used to investigate the expression levels of miR-147 in 32 paired NSCLC tissues and their adjacent normal lung tissues, sera of 122 control and 87 NSCLC patients. The correlation of serum miR-147 expression level with clinicopathological characteristics, and the prognosis of NSCLC patients was statistically evaluated. Results: MiR-147 was significantly down-regulated in NSCLC tissues than in paired adjacent normal tissues, and in sera of NSCLC patients than in sera of control patients. In addition, serum miR-147 was markedly down-regulated in advanced NSCLC patients and the patients with lymph node metastasis (LNM). Low serum miR-147 expression level was found to be significantly correlated with TNM stage, LNM and tumor size. Statistical analysis showed that patients with low serum miR-147 had much worse overall survival, and low serum miR-147 expression level was an independent prognostic factor for poor prognosis for NSCLC. Conclusion: Low serum miR-147 expression level may be a useful biomarker for patients with NSCLC.
© 2015 Taylor & Francis. This provisional PDF corresponds to the article as it appeared upon acceptance. Fully formatted PDF and full text (HTML) versions will be made available soon. DISCLAIMER: The ideas and opinions expressed in the journal’s Just Accepted articles do not necessarily reflect those of Taylor & Francis (the Publisher), the Editors or the journal. The Publisher does not assume any responsibility for any injury and/or damage to persons or property arising from or related to any use of the material contained in these articles. The reader is advised to check the appropriate medical literature and the product information currently provided by the manufacturer of each drug to be administered to verify the dosages, the method and duration of administration, and contraindications. It is the responsibility of the treating physician or other health care professional, relying on his or her independent experience and knowledge of the patient, to determine drug dosages and the best treatment for the patient. Just Accepted articles have undergone full scientific review but none of the additional editorial preparation, such as copyediting, typesetting, and proofreading, as have articles published in the traditional manner. There may, therefore, be errors in Just Accepted articles that will be corrected in the final print and final online version of the article. Any use of the Just Accepted articles is subject to the express understanding that the papers have not yet gone through the full quality control process prior to publication.
Title: Serum level of microRNA-147 as diagnostic biomarker in human non-small cell lung caner Guangmin Chu1*, Jianbo Zhang1, Xiaobing Chen2 1: Department of Pathology, Henan Tumor Hospital, zhengzhou, 450008, China 2: Department of Oncology, Henan Tumor Hospital, zhengzhou, 450008, China
Downloaded by [Monash University Library] at 02:32 24 November 2015
Short title: miR-147 in NSCLC
*: Corresponding Author Guangmin Chu, M.D. 127 Dongming Rd Department of Pathology Henan Tumor Hospital Zhengzhou, 450008, China Phone: +86-0371-65587157 Email: [email protected]
Abstract Objectives: In this study, we intended to examine the gene expression level and the clinical significance of microRNA-147 (miR-147) in cancer tissues and sera of patients with non-small cell lung cancer (NSCLC). Methods: Quantitative real-time PCR (qRT-PCR) was used to investigate the expression levels of miR-147 in 32 paired NSCLC tissues and their adjacent normal lung tissues, sera of 122 control and
Downloaded by [Monash University Library] at 02:32 24 November 2015
87 NSCLC patients. The correlation of serum miR-147 expression level with clinicopathological characteristics, and the prognosis of NSCLC patients was statistically evaluated. Results: MiR-147 was significantly down-regulated in NSCLC tissues than in paired adjacent normal tissues, and in sera of NSCLC patients than in sera of control patients. In addition, serum miR-147 was markedly down-regulated in advanced NSCLC patients and the patients with lymph node metastasis (LNM). Low serum miR-147 expression level was found to be significantly correlated with TNM stage, LNM and tumor size. Statistical analysis showed that patients with low serum miR147 had much worse overall survival, and low serum miR-147 expression level was an independent prognostic factor for poor prognosis for NSCLC. Conclusion: Low serum miR-147 expression level may be a useful biomarker for patients with NSCLC. Keywords: NSCLC, miR-147, prognosis, overall survival, serum miRNA
Introduction Lung cancer is one of the most common cancers among both male and female [1]. In the United States alone, nearly quarter million new cases of lung cancer will be diagnosed and more than 150,000 people will die from lung cancer every year [1]. Non-small-cell lung cancer (NSCLC) is the most common form of lung cancer, accounting for ~85% of total patients with lung cancer [2]. Though great advances had been made to improve NSCLC early diagnosis and treatment, the 5-year overall survival was only slightly improved for the past decades, due to the complexity of
Downloaded by [Monash University Library] at 02:32 24 November 2015
carcinogenesis among patients with NSCLC [3]. Therefore, it is very important to examine the molecular signaling network of NSCLC, thus to unravel the genes or prognostic factors responsible for tumorigenesis and progression of NSCLC. MicroRNAs (miRNAs) are classes of small-length (18~22 nt) non-coding RNAs that are participating in the various biological or pathological processes by binding the 3’-UTR of target mRNA [4-6]. In human NSCLC, miRNAs are actively involved in various aspects of cancer regulations and many of them can be used as efficient cancer biomarkers due to their aberrant expressions [7-9]. For example, miR-181b was found to be significantly down-regulated in NSCLC tissues, and the low miR-181b level was closely correlated with poor prognosis and survivals of NSCLC patients [10]. MiR-375 was also found to be downregulated in majority of the NSCLC tissues (
ISSN: 1061-186X (Print) 1029-2330 (Online) Journal homepage: http://www.tandfonline.com/loi/idrt20
Serum level of microRNA-147 as diagnostic biomarker in human non-small cell lung caner Guangmin Chu, Jianbo Zhang & Xiaobing Chen To cite this article: Guangmin Chu, Jianbo Zhang & Xiaobing Chen (2015): Serum level of microRNA-147 as diagnostic biomarker in human non-small cell lung caner, Journal of Drug Targeting, DOI: 10.3109/1061186X.2015.1116539 To link to this article: http://dx.doi.org/10.3109/1061186X.2015.1116539
Accepted author version posted online: 18 Nov 2015.
Submit your article to this journal
Article views: 3
View related articles
View Crossmark data
Full Terms & Conditions of access and use can be found at http://www.tandfonline.com/action/journalInformation?journalCode=idrt20 Download by: [Monash University Library]
Date: 24 November 2015, At: 02:32
Just Accepted by Journal of Drug Targeting Serum level of microRNA-147 as diagnostic biomarker in human nonsmall cell lung caner Guangmin Chu, Jianbo Zhang, Xiaobing Chen doi: 10.3109/1061186X.2015.1116539 Abstract
Downloaded by [Monash University Library] at 02:32 24 November 2015
Objectives: In this study, we intended to examine the gene expression level and the clinical significance of microRNA-147 (miR-147) in cancer tissues and sera of patients with non-small cell lung cancer (NSCLC). Methods: Quantitative real-time PCR (qRT-PCR) was used to investigate the expression levels of miR-147 in 32 paired NSCLC tissues and their adjacent normal lung tissues, sera of 122 control and 87 NSCLC patients. The correlation of serum miR-147 expression level with clinicopathological characteristics, and the prognosis of NSCLC patients was statistically evaluated. Results: MiR-147 was significantly down-regulated in NSCLC tissues than in paired adjacent normal tissues, and in sera of NSCLC patients than in sera of control patients. In addition, serum miR-147 was markedly down-regulated in advanced NSCLC patients and the patients with lymph node metastasis (LNM). Low serum miR-147 expression level was found to be significantly correlated with TNM stage, LNM and tumor size. Statistical analysis showed that patients with low serum miR-147 had much worse overall survival, and low serum miR-147 expression level was an independent prognostic factor for poor prognosis for NSCLC. Conclusion: Low serum miR-147 expression level may be a useful biomarker for patients with NSCLC.
© 2015 Taylor & Francis. This provisional PDF corresponds to the article as it appeared upon acceptance. Fully formatted PDF and full text (HTML) versions will be made available soon. DISCLAIMER: The ideas and opinions expressed in the journal’s Just Accepted articles do not necessarily reflect those of Taylor & Francis (the Publisher), the Editors or the journal. The Publisher does not assume any responsibility for any injury and/or damage to persons or property arising from or related to any use of the material contained in these articles. The reader is advised to check the appropriate medical literature and the product information currently provided by the manufacturer of each drug to be administered to verify the dosages, the method and duration of administration, and contraindications. It is the responsibility of the treating physician or other health care professional, relying on his or her independent experience and knowledge of the patient, to determine drug dosages and the best treatment for the patient. Just Accepted articles have undergone full scientific review but none of the additional editorial preparation, such as copyediting, typesetting, and proofreading, as have articles published in the traditional manner. There may, therefore, be errors in Just Accepted articles that will be corrected in the final print and final online version of the article. Any use of the Just Accepted articles is subject to the express understanding that the papers have not yet gone through the full quality control process prior to publication.
Title: Serum level of microRNA-147 as diagnostic biomarker in human non-small cell lung caner Guangmin Chu1*, Jianbo Zhang1, Xiaobing Chen2 1: Department of Pathology, Henan Tumor Hospital, zhengzhou, 450008, China 2: Department of Oncology, Henan Tumor Hospital, zhengzhou, 450008, China
Downloaded by [Monash University Library] at 02:32 24 November 2015
Short title: miR-147 in NSCLC
*: Corresponding Author Guangmin Chu, M.D. 127 Dongming Rd Department of Pathology Henan Tumor Hospital Zhengzhou, 450008, China Phone: +86-0371-65587157 Email: [email protected]
Abstract Objectives: In this study, we intended to examine the gene expression level and the clinical significance of microRNA-147 (miR-147) in cancer tissues and sera of patients with non-small cell lung cancer (NSCLC). Methods: Quantitative real-time PCR (qRT-PCR) was used to investigate the expression levels of miR-147 in 32 paired NSCLC tissues and their adjacent normal lung tissues, sera of 122 control and
Downloaded by [Monash University Library] at 02:32 24 November 2015
87 NSCLC patients. The correlation of serum miR-147 expression level with clinicopathological characteristics, and the prognosis of NSCLC patients was statistically evaluated. Results: MiR-147 was significantly down-regulated in NSCLC tissues than in paired adjacent normal tissues, and in sera of NSCLC patients than in sera of control patients. In addition, serum miR-147 was markedly down-regulated in advanced NSCLC patients and the patients with lymph node metastasis (LNM). Low serum miR-147 expression level was found to be significantly correlated with TNM stage, LNM and tumor size. Statistical analysis showed that patients with low serum miR147 had much worse overall survival, and low serum miR-147 expression level was an independent prognostic factor for poor prognosis for NSCLC. Conclusion: Low serum miR-147 expression level may be a useful biomarker for patients with NSCLC. Keywords: NSCLC, miR-147, prognosis, overall survival, serum miRNA
Introduction Lung cancer is one of the most common cancers among both male and female [1]. In the United States alone, nearly quarter million new cases of lung cancer will be diagnosed and more than 150,000 people will die from lung cancer every year [1]. Non-small-cell lung cancer (NSCLC) is the most common form of lung cancer, accounting for ~85% of total patients with lung cancer [2]. Though great advances had been made to improve NSCLC early diagnosis and treatment, the 5-year overall survival was only slightly improved for the past decades, due to the complexity of
Downloaded by [Monash University Library] at 02:32 24 November 2015
carcinogenesis among patients with NSCLC [3]. Therefore, it is very important to examine the molecular signaling network of NSCLC, thus to unravel the genes or prognostic factors responsible for tumorigenesis and progression of NSCLC. MicroRNAs (miRNAs) are classes of small-length (18~22 nt) non-coding RNAs that are participating in the various biological or pathological processes by binding the 3’-UTR of target mRNA [4-6]. In human NSCLC, miRNAs are actively involved in various aspects of cancer regulations and many of them can be used as efficient cancer biomarkers due to their aberrant expressions [7-9]. For example, miR-181b was found to be significantly down-regulated in NSCLC tissues, and the low miR-181b level was closely correlated with poor prognosis and survivals of NSCLC patients [10]. MiR-375 was also found to be downregulated in majority of the NSCLC tissues (
