Nucl Med Mol Imaging (2011) 45:15–20 DOI 10.1007/s13139-010-0061-9
ORIGINAL ARTICLE
Quantitative Measurement of Serum Hepatitis B Surface Antigen Using an Immunoradiometric Assay in Chronic Hepatitis B Hyun-Woo Kwon & Ho-Young Lee & Seog Gyun Kim & Won Kim & Yong Jin Jung & Keon Wook Kang & June-Key Chung & Myung Chul Lee & Dong Soo Lee
Received: 15 September 2010 / Accepted: 18 October 2010 / Published online: 16 November 2010 # Korean Society of Nuclear Medicine 2010
Abstract Purpose Measurement of serum hepatitis B virus surface antigen (HBsAg) levels is important for the management of chronic hepatitis B patients in terms of monitoring response to antiviral therapy. This study aimed to evaluate the diagnostic performance of a new diagnostic kit, which quantitatively measures serum HBsAg level using an immunoradiometric assay (IRMA)-based method. Measurements were compared with those obtained using a chemiluminescent microparticle immunoassay (CMIA)-based method. Methods The blood samples of 96 patients with chronic hepatitis B were used in this study. Copy numbers of serum hepatitis B virus (HBV) DNA were determined in 23 of these samples. The correlation between and the concor-
dance of IRMA and CMIA results were determined using Pearson’s correlation coefficients. P values of 0.05 were considered to be statistically significant throughout. Results Laboratory diagnoses based on IRMA were wholly in agreement with those based on CMIA. Furthermore, serum HBsAg levels by IRMA were found to be highly correlated with those determined by CMIA (correlation coefficient R2 = 0.838, p
ORIGINAL ARTICLE
Quantitative Measurement of Serum Hepatitis B Surface Antigen Using an Immunoradiometric Assay in Chronic Hepatitis B Hyun-Woo Kwon & Ho-Young Lee & Seog Gyun Kim & Won Kim & Yong Jin Jung & Keon Wook Kang & June-Key Chung & Myung Chul Lee & Dong Soo Lee
Received: 15 September 2010 / Accepted: 18 October 2010 / Published online: 16 November 2010 # Korean Society of Nuclear Medicine 2010
Abstract Purpose Measurement of serum hepatitis B virus surface antigen (HBsAg) levels is important for the management of chronic hepatitis B patients in terms of monitoring response to antiviral therapy. This study aimed to evaluate the diagnostic performance of a new diagnostic kit, which quantitatively measures serum HBsAg level using an immunoradiometric assay (IRMA)-based method. Measurements were compared with those obtained using a chemiluminescent microparticle immunoassay (CMIA)-based method. Methods The blood samples of 96 patients with chronic hepatitis B were used in this study. Copy numbers of serum hepatitis B virus (HBV) DNA were determined in 23 of these samples. The correlation between and the concor-
dance of IRMA and CMIA results were determined using Pearson’s correlation coefficients. P values of 0.05 were considered to be statistically significant throughout. Results Laboratory diagnoses based on IRMA were wholly in agreement with those based on CMIA. Furthermore, serum HBsAg levels by IRMA were found to be highly correlated with those determined by CMIA (correlation coefficient R2 = 0.838, p
