Peptides,Vol. 12, pp. 11-15. ©PergamonPress plc, 1991. Printedin the U.S.A.
0196-9781/91 $3.00 + .00
Isolation, Structural Characterization and Pharmacological Activity of Dog Neuromedin U F I N B A R R O ' H A R T E , C H A R L E S S. B O C K M A N , * P E T E R W. ABEL* A N D J. M I C H A E L C O N L O N l
Regulatory Peptide Center, Department of Biomedical Sciences and ~Department of Pharmacology Creighton University School of Medicine, Omaha, NE 68178 Received 30 July 1990
O'HARTE, F., C. S. BOCKMAN, P. W. ABEL AND J. M. CONLON. Isolation, structuralcharacterizationand pharmacological activityof dog neuromedin U. PEPTIDES 12(1) 11-15, 1991.--The neuromedin U-like immunoreactivity in an extract of dog small intestine was resolved by reversed-phase HPLC into two molecular forms. The primary structure of the larger form (NMU25) was established as: Phe-Arg-Leu-Asp-G~u-G~u-Phe-G~n-G~y-Pr~1~-~e-A~a-Ser-G~n-Va~Arg-Arg-G~n-Phe-Leu2~-Phe-Arg~Pr~ Arg-Asn-NH:. This sequence shows five substitutions relative to pig neuromedin U-25. The primary structure of the second peptide (NMU-8) was established as: pGlu-Phe-Leu-Phe-Arg-Pro-Arg-Asn-NH2. The sequence contains the substitution pGlu for Tyrt compared with pig neuromedin U-8. The potency of synthetic dog NMU-8 in contracting smooth muscle from the rat uterus (ECso 10 - 2 nM; mean_+S.E., n=6) was not significantly different from the corresponding potency of pig NMU-8 (EC5o 16-+5 riM) but the maximum response produced by the dog peptide was greater (58%; p
0196-9781/91 $3.00 + .00
Isolation, Structural Characterization and Pharmacological Activity of Dog Neuromedin U F I N B A R R O ' H A R T E , C H A R L E S S. B O C K M A N , * P E T E R W. ABEL* A N D J. M I C H A E L C O N L O N l
Regulatory Peptide Center, Department of Biomedical Sciences and ~Department of Pharmacology Creighton University School of Medicine, Omaha, NE 68178 Received 30 July 1990
O'HARTE, F., C. S. BOCKMAN, P. W. ABEL AND J. M. CONLON. Isolation, structuralcharacterizationand pharmacological activityof dog neuromedin U. PEPTIDES 12(1) 11-15, 1991.--The neuromedin U-like immunoreactivity in an extract of dog small intestine was resolved by reversed-phase HPLC into two molecular forms. The primary structure of the larger form (NMU25) was established as: Phe-Arg-Leu-Asp-G~u-G~u-Phe-G~n-G~y-Pr~1~-~e-A~a-Ser-G~n-Va~Arg-Arg-G~n-Phe-Leu2~-Phe-Arg~Pr~ Arg-Asn-NH:. This sequence shows five substitutions relative to pig neuromedin U-25. The primary structure of the second peptide (NMU-8) was established as: pGlu-Phe-Leu-Phe-Arg-Pro-Arg-Asn-NH2. The sequence contains the substitution pGlu for Tyrt compared with pig neuromedin U-8. The potency of synthetic dog NMU-8 in contracting smooth muscle from the rat uterus (ECso 10 - 2 nM; mean_+S.E., n=6) was not significantly different from the corresponding potency of pig NMU-8 (EC5o 16-+5 riM) but the maximum response produced by the dog peptide was greater (58%; p
