Pulmonary Pharmacology (1990) 3 155-I60

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PULMONARY PHARMACOLOGY

Epithelium-Derived PGE2 Inhibits the Contractile Response to Cholinergic Stimulation in Isolated Ferret Trachea A . Ullman*, G . Ciabattoni$, C . G . Lofdahlt, A . Lindent, N . Svedmyr*, B . E . Skooght Departments of Clinical Pharmacology* and Pulmonary Medicinet, Gothenburg University, Sweden and Department of Pharmacology$, Catholic University, Rome, Italy SUMMARY. We have previously reported that epithelium-dependent inhibitory factors, both prostanoids and nonprostanoids, can be activated by electrical field stimulation (EFS) and direct nerve stimulation (DNS) in an in vitro nerve-muscle preparation of ferret trachea. In this study we set out to compare the release of the inhibitory prostanoids, PGE 2 and PGI 2 , in preparations with intact and with removed epithelium . Ferret tracheae were mounted in organ baths and phasic contractions were induced by EFS and DNS . The bath concentrations of PGE 2 and the PGI 2 -metabolite 6-keto-PGF,, were measured using radioimmuno assays . The gradual decrease in contractile response to DNS, 2 Hz for 120 min, was 95 ± 2% of baseline (mean ± SEM) in preparations with intact epithelium compared with 29±8% in epithelium-denuded preparations (p

Epithelium-derived PGE2 inhibits the contractile response to cholinergic stimulation in isolated ferret trachea.

We have previously reported that epithelium-dependent inhibitory factors, both prostanoids and non-prostanoids, can be activated by electrical field s...
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