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Effects of glyphosate and its formulation, Roundup , on reproduction in zebrafish (Danio rerio). Tamsyn M Uren Webster, Lauren V Laing, Hannah Florance, and Eduarda M. Santos Environ. Sci. Technol., Just Accepted Manuscript • Publication Date (Web): 23 Dec 2013 Downloaded from http://pubs.acs.org on December 30, 2013

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Environmental Science & Technology

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Effects of glyphosate and its formulation,

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Roundup®, on reproduction in zebrafish (Danio

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rerio).

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Tamsyn. M. Uren Webster1*, Lauren V. Laing1, Hannah Florance1 and Eduarda M. Santos1*

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1. Biosciences, College of Life & Environmental Sciences, Geoffrey Pope Building, University

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of Exeter, Exeter, EX4 4QD

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* Corresponding authors

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ABSTRACT

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Roundup®, and its active ingredient glyphosate, are among the most widely used herbicides

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worldwide, and may contaminate surface waters. Research suggests both Roundup® and

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glyphosate induce oxidative stress in fish, and may also cause reproductive toxicity in

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mammalian systems. We aimed to investigate the reproductive effects of Roundup® and

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glyphosate in fish, and the potential associated mechanisms of toxicity. To do this, we conducted

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a 21-day exposure of breeding zebrafish (Danio rerio) to 0.01, 0.5 and 10 mg/L (glyphosate acid

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equivalent) Roundup® and 10 mg/L glyphosate. 10 mg/L glyphosate reduced egg production, but

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not fertilisation rate in breeding colonies. Both 10 mg/L Roundup® and glyphosate increased

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early-stage embryo mortalities and premature hatching. However, exposure during

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embryogenesis alone did not increase embryo mortality, suggesting that this effect was caused

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primarily by exposure during gametogenesis. Transcript profiling of the gonads revealed 10

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mg/L Roundup® and glyphosate induced changes in the expression of cyp19a1 and esr1 in the

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ovary, and hsd3b2, cat and sod1 in the testis. Our results demonstrate that these chemicals cause

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reproductive toxicity in zebrafish, although only at high concentrations unlikely to occur in the

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environment, and likely mechanisms of toxicity include disruption of the steroidogenic

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biosynthesis pathway and oxidative stress.

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Environmental Science & Technology

INTRODUCTION

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Glyphosate is extensively used worldwide, topping lists of agricultural herbicide usage in

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Europe [1] and the US [2]. It is a broad-spectrum, post emergence herbicide, which acts by

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binding phosphoenolpyruvate, the substrate of EPSP synthase, and subsequently inhibiting

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aromatic amino acid synthesis via the shikimate pathway in plants [3, 4]. Glyphosate is generally

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applied as part of a formulated product, the most widely used of which are the Roundup®

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herbicides. Roundup® formulations contain glyphosate in the form of an isoproylamine salt,

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which aids solubility but does not affect its properties as the active ingredient, together with

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various adjuvants which enhance its herbicidal properties. One of the most important and

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commonly used adjuvants is polyethoxylated tallow amine (POEA), a surfactant that enhances

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penetration of glyphosate through the plant cuticle [5, 6]. Glyphosate and Roundup® are also

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extensively used as domestic and urban-area weed-killers [2]. Commercial glyphosate

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formulations vary in composition with country and purpose and the properties of these

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formulations, including their toxicity, can be compared using the concentration of glyphosate

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present, expressed as glyphosate acid equivalent (a.e.).

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Glyphosate is known to strongly adsorp to soil, where it is subject to microbial degradation.

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This is one of glyphosate’s advantageous herbicidal properties, limiting agricultural input to

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surface waters in ideal conditions. However, pulses of contamination can be expected when

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rainfall occurs directly after application and when flood events increase river sediment load [6].

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Urban runoff and wastewater treatment effluent also account for considerable glyphosate input

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into rivers [7]. Despite its widespread use, concentrations of glyphosate, or its associated

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formulation components, are not routinely monitored in surface waters. However, glyphosate

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concentrations worldwide have been regularly reported to occur up to ~10-15 µg/L in rivers

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[e.g.8, 9]. Considerably higher peaks in concentration, in the high µg/L range, have also been

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measured, but are mainly associated with direct aquatic application, and in isolated wetland

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environments [6, 10].

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Although the target mechanism of action of glyphosate and glyphosate-based formulations is

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specific to plants, they have been shown to induce diverse biological effects in a range of non-

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target organisms. In fish, much of previous research assessing effects of Roundup® and

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glyphosate has focused on their induction of oxidative stress through ROS generation and/or

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interference with cellular antioxidant production. Short-term exposures (up to 6 days) to 1-20

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mg/L of several Roundup® formulations in a number of fish species altered levels of cellular

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antioxidants and induced oxidative damage of DNA, lipids and proteins [e.g. 11, 12-15].

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Environmentally relevant concentrations of Roundup®, glyphosate and POEA have also induced

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DNA damage in blood and liver cells of eel and catfish after up to 9 days exposure [16-19].

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Other studies have found that Roundup®, and in some cases glyphosate, induce other effects in

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fish including neurotoxicity and immunotoxicity [e.g. 20, 21, 22]. Similar evidence of Roundup®

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and glyphosate toxicity has been found for other vertebrate species, and demonstrated effects

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include occurrence of developmental abnormalities, especially in amphibians [23, 24]. Roundup®

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formulations have largely been found to be more toxic than pure glyphosate. The inherent

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toxicity of POEA [17, 23], and potentially other formulation components, is likely to contribute

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to this, although a modulating effect on glyphosate toxicity is also possible. Few studies,

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however, have directly compared equivalent concentrations of Roundup® and glyphosate.

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The potential of Roundup® to disrupt the endocrine system in vitro has been demonstrated in

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mammalian cell lines. In mouse Leydig cells, sub-lethal concentrations of Roundup® (from 25

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mg/L) altered the transcription and activity of steroidogenic acute regulatory protein (StAR),

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resulting in disruption of progesterone production [25]. A number of studies demonstrated

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consistent inhibition of aromatase activity by Roundup® in various human cell lines. The

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concentrations required to cause these effects varied depending on the cell type and formulation,

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but included from 4 mg/L in liver cells and 72 mg/L in placental and embryonic cells [26-28].

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Less consistently, and to a smaller extent than Roundup®, aromatase inhibition by glyphosate

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alone has also been reported (approximately 10 fold higher concentrations) [28]. Additionally,

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both glyphosate and Roundup® were reported to reduce testosterone production in rat testicular

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cells at concentrations from 0.36 mg/L [29], but it is difficult to relate these results to the

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potential effects of these chemicals in vivo. Few studies have investigated the effects of

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glyphosate and its commercial formulations on the endocrine system in vivo. Drakes treated with

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5 and 100 mg Roundup®/kg (body weight) exhibited reduced levels of testosterone,

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corresponding with alterations in testis structure. In rats, maternal and juvenile treatment from 5

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and 50 mg/kg (body weight) of Roundup® impaired male reproductive development, with effects

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including alteration in testis structure, sperm production and sex steroid production [30-32].

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Reproductive effects of Roundup® and glyphosate in fish have seldom been investigated and

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are far from clear. While no evidence of altered gonadal development was evident in juvenile

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stickleback exposed to 0.1-100 µg/L glyphosate [33], treatment with 3.6 mg/L Roundup® had

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some negative impact on offspring production in Silver catfish [34]. The mechanisms

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contributing to this reproductive effect have not been investigated.

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Given the extensive usage of glyphosate based herbicides, there is a clear potential for the

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environmental exposure of fish populations to glyphosate together with associated formulation

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products, which may modify its toxicity. This study aimed to examine the effects of Roundup®

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formulation on reproduction in fish and to determine to what extend these effects were associated

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with the toxicity of glyphosate alone. To do this, we conducted a 21 day reproductive test in

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breeding colonies of zebrafish, to determine if reproduction, embryo development and embryo

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survival, were affected by exposure to 0.01, 0.5 and 10 mg/L (glyphosate acid equivalent)

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Roundup® and 10 mg/L glyphosate. The two lower Roundup® concentrations included in this

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study were chosen to represent concentrations that can be expected to occur in the environment

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regularly (0.01 mg/L) and during occasional peak contamination events (0.5 mg/L). The highest

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concentration tested (10 mg/L) is unlikely to occur in surface waters, and was included to

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facilitate the analysis of the mechanisms of toxicity. We included a treatment group exposed to

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10 mg/L glyphosate alone to allow for a direct comparison of its mechanisms of toxicity with the

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equivalent a.e. concentration of Roundup®. We hypothesised that the mechanisms of toxicity

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resulting in effects on reproduction might include oxidative stress and disruption of steroid

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biosynthesis, and to investigate this we conducted transcript profiling of a suite of genes

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involved in these processes in the gonads.

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MATERIALS AND METHODS

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Fish maintenance

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Colonies of 4 male and 4 female adult (20 week old) WIK strain zebrafish were established in

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individual 15 L glass tanks and allowed to breed naturally during a 7 day acclimation period.

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Fish were maintained according to Paull et al. [35] and a full description of husbandry

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procedures is provided in the supporting information.

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Chemical exposures

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Chemical exposure was conducted via a flow through system for a period of 21 days in

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accordance with OECD guidelines for fish reproductive tests, preceded by a 10 day pre-exposure

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period [36]. The treatment groups consisted of three concentrations of Roundup®; 0.01, 0.5 and

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10 mg/L glyphosate acid equivalent (using Roundup® GC liquid glyphosate concentrate

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containing 120 g/L glyphosate acid; Monsanto, Cambridge, UK); 10 mg/L glyphosate (analytical

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grade; Molekula, Wimborne, UK); and a control group. Each treatment group was comprised of

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three replicate breeding colonies (4 males and 4 females) in 15L tanks. Water samples were

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collected from each tank on days 7, 14 and 21 of the exposure period and stored at -20 °C prior

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to chemical analysis. Details of the analytical chemistry procedures are provided in supplemental

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material.

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Reproductive test and embryo exposures

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Group spawning occurred daily at dawn and eggs were collected 1 hour post fertilisation (hpf),

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rinsed thoroughly to remove detritus and incubated in water containing the same chemical

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exposure concentrations as their tank of origin, at 28 °C. Exposure water for the embryo

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experiments was made according to the ISO 7346−3:1996 guidelines [37], fully oxygenated and

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supplemented with 2.5 µl/L of the antifungal agent Methylene Blue (Interpet; Dorking, UK) to

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avoid mortalities caused by fungal infections. The eggs from each colony were examined using

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light microscopy between 2 ½ and 3 ½ hours after dawn, when all fertilised eggs had reached at

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least the 16-cell stage during early cleavage [38], and the total number of fertilised and

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unfertilised eggs were quantified on each day throughout the pre-exposure and exposure periods.

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During the 21-day chemical exposure, fertilised eggs displaying cellular necrosis were counted

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and recorded as early-stage mortalities (

Effects of glyphosate and its formulation, roundup, on reproduction in zebrafish (Danio rerio).

Roundup and its active ingredient glyphosate are among the most widely used herbicides worldwide and may contaminate surface waters. Research suggests...
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