0021-972X/78/4703-0665I02.00/0 Journal of Clinical Endocrinology and Metabolism w Copyright © 1978 by The Endocrine Society
Vol. 47, No. 3 Printed in U.S.A.
Comments v
Correlations between Plasma Renin Activity, Angiotensin II, and Aldosterone KARI J. KOSUNEN AND ARTO PAKARINEN Department of Clinical Chemistry, University of Helsinki, Helsinki; and the Department of Clinical Chemistry, University of Oulu, Oulu, Finland ABSTRACT. To obtain more information about the relationships of the components of the renin-angiotensin-aldosterone system, we measured plasma renin activity (PRA) and the concentrations of plasma angiotensin II (All) and aldosterone (PA) in 36 healthy 19-22yr-old students, both after a night's bed rest and after 2 h of ambulation. The correlations of these parameters were calculated. PRA, All, and PA were determined by RIAs. The values of All after rest correlated positively both with PRA (r = 0.80; P < 0.001) and with PA (r = 0.51; P < 0.01). All also showed a good correlation with PRA (r = 0.77; P < 0.001) and with PA (r = 0.67; P
K
•
n 0
p
, , , , , , , 20 40 60 ANGIOTENSIN 0 , ng/l
0
200 400 600 800 ALDOSTERONE, pmol/l
FIG. 2. The correlation between the values of PRA and All (A) and All and PA (B) at 0800 h after a night's rest (O) and at 1000 h after 2 h of ambulation (•).
components of the renin-angiotensin-aldosterone system (Fig. 1). The mean PRA increased from the rest value of 2.1 ± 0.7 to 3.2 ± 0.9 (SD) /xg/liter/h (P < 0.001) after ambulation. The respective increase in plasma All was from 31.1 ± 10.7 to 42.4 ± 12.8 ng/liter (P < 0.001), and in PA was from 162 ± 93 to 312 ± 217 pmol/liter (P < 0.001). The following correlations were observed between the rest values (at 0800 h) of PRA, All, and PA (Fig. 2): PRA-AII, r = 0.80, P < 0.001 (n = 36); and AII-PA, r = 0.51, P < 0.01 (n = 27). The correlations between the values after 2 h of ambulation (at 1000 h) were: PRA-AII, r = 0.77, P < 0.001 (n = 36); and AII-PA, r = 0.67, P < 0.001 (n = 27). Positive correlations were also found between the increases in PRA and All (r = 0.44; P < 0.01) and between All and PA (r = 0.53; P < 0.01). A multiple regression analysis gave the following regression equations: [X]PA = 4.04 [X]PRA + 3.30 [X]AII + 52.08 (r = 0.37), for
Our results support the view that the values of PRA and All parallel each other in normal physiological circumstances. It is not probable that ACTH plays any significant role in the orthostatism-induced increase in PA, as ambulation does not increase serum cortisol in normal human subjects (7). On the other hand, Sassard et al. (3) have concluded that the slight increase in plasma potassium concentration during active orthostatism is a weaker stimulus for aldosterone secretion than that caused by the renin-angiotensin system. The good positive correlation between the concentrations of All and PA found in the present study, both after a night's bed rest and after 2 h of ambulation, seems to indicate that All has an important role in the basal as well as in the posture-stimulated secretion of aldosterone in normal man.
References 1. CAIN, M. D., J. P. COGHLAN, AND K. J. CATT, Measurement
of angiotensin II in blood by radioimmunoassay, Clin Chim Ada 39: 21, 1972. 2. SUNDSFJORD, J. A., S. B. STROMME, AND A. AAKVAAG, Plasma
aldosterone (PA), plasma renin activity (PRA) and cortisol (PF) during exercise, In Howald, H., and J. R. Poortmans (eds.), Metabolic Adaptation to Prolonged Physical Exercise, Birkhauser Verlag, Basel, 1975, p. 308. 3. SASSARD, J., M. VINCENT, G. ANNAT, AND C. A. BIZOLLON, A
kinetic study of plasma renin and aldosterone during changes, J Clin Endocrinol Metab 42: 20, 1976. 4. KOSUNEN, K. J., AND A. PAKARINEN, Plasma renin, angiotensin II, and plasma and urinary aldosterone in running exercise, JAppl Physiol 41: 26, 1976. 5. KOSUNEN, K. J., A simple method for measurement of angiotensin II in plasma, Scand J Clin Lab Invest 36: 467, 1976. 6. PAKARINEN, A., L. KOSKINEN, AND H. ADLERCREUTZ, Eval-
uation of radioimmunological methods for assay of plasma and urinary aldosterone, Scand J Clin Lab Invest 36: 331, 1976. 7. WILLIAMS, G. H , J. P. CAIN, R. G. DLUHY, AND R. H.
UNDERWOOD, Studies of the control of plasma aldosterone concentration in normal man. I. Response to posture, acute and chronic volume depletion and sodium loading, J Clin Invest Eil: 1731, 1972.
The Endocrine Society. Downloaded from press.endocrine.org by [${individualUser.displayName}] on 15 November 2015. at 02:26 For personal use only. No other uses without permission. . All rights reserved.
Vol. 47, No. 3 Printed in U.S.A.
Comments v
Correlations between Plasma Renin Activity, Angiotensin II, and Aldosterone KARI J. KOSUNEN AND ARTO PAKARINEN Department of Clinical Chemistry, University of Helsinki, Helsinki; and the Department of Clinical Chemistry, University of Oulu, Oulu, Finland ABSTRACT. To obtain more information about the relationships of the components of the renin-angiotensin-aldosterone system, we measured plasma renin activity (PRA) and the concentrations of plasma angiotensin II (All) and aldosterone (PA) in 36 healthy 19-22yr-old students, both after a night's bed rest and after 2 h of ambulation. The correlations of these parameters were calculated. PRA, All, and PA were determined by RIAs. The values of All after rest correlated positively both with PRA (r = 0.80; P < 0.001) and with PA (r = 0.51; P < 0.01). All also showed a good correlation with PRA (r = 0.77; P < 0.001) and with PA (r = 0.67; P
K
•
n 0
p
, , , , , , , 20 40 60 ANGIOTENSIN 0 , ng/l
0
200 400 600 800 ALDOSTERONE, pmol/l
FIG. 2. The correlation between the values of PRA and All (A) and All and PA (B) at 0800 h after a night's rest (O) and at 1000 h after 2 h of ambulation (•).
components of the renin-angiotensin-aldosterone system (Fig. 1). The mean PRA increased from the rest value of 2.1 ± 0.7 to 3.2 ± 0.9 (SD) /xg/liter/h (P < 0.001) after ambulation. The respective increase in plasma All was from 31.1 ± 10.7 to 42.4 ± 12.8 ng/liter (P < 0.001), and in PA was from 162 ± 93 to 312 ± 217 pmol/liter (P < 0.001). The following correlations were observed between the rest values (at 0800 h) of PRA, All, and PA (Fig. 2): PRA-AII, r = 0.80, P < 0.001 (n = 36); and AII-PA, r = 0.51, P < 0.01 (n = 27). The correlations between the values after 2 h of ambulation (at 1000 h) were: PRA-AII, r = 0.77, P < 0.001 (n = 36); and AII-PA, r = 0.67, P < 0.001 (n = 27). Positive correlations were also found between the increases in PRA and All (r = 0.44; P < 0.01) and between All and PA (r = 0.53; P < 0.01). A multiple regression analysis gave the following regression equations: [X]PA = 4.04 [X]PRA + 3.30 [X]AII + 52.08 (r = 0.37), for
Our results support the view that the values of PRA and All parallel each other in normal physiological circumstances. It is not probable that ACTH plays any significant role in the orthostatism-induced increase in PA, as ambulation does not increase serum cortisol in normal human subjects (7). On the other hand, Sassard et al. (3) have concluded that the slight increase in plasma potassium concentration during active orthostatism is a weaker stimulus for aldosterone secretion than that caused by the renin-angiotensin system. The good positive correlation between the concentrations of All and PA found in the present study, both after a night's bed rest and after 2 h of ambulation, seems to indicate that All has an important role in the basal as well as in the posture-stimulated secretion of aldosterone in normal man.
References 1. CAIN, M. D., J. P. COGHLAN, AND K. J. CATT, Measurement
of angiotensin II in blood by radioimmunoassay, Clin Chim Ada 39: 21, 1972. 2. SUNDSFJORD, J. A., S. B. STROMME, AND A. AAKVAAG, Plasma
aldosterone (PA), plasma renin activity (PRA) and cortisol (PF) during exercise, In Howald, H., and J. R. Poortmans (eds.), Metabolic Adaptation to Prolonged Physical Exercise, Birkhauser Verlag, Basel, 1975, p. 308. 3. SASSARD, J., M. VINCENT, G. ANNAT, AND C. A. BIZOLLON, A
kinetic study of plasma renin and aldosterone during changes, J Clin Endocrinol Metab 42: 20, 1976. 4. KOSUNEN, K. J., AND A. PAKARINEN, Plasma renin, angiotensin II, and plasma and urinary aldosterone in running exercise, JAppl Physiol 41: 26, 1976. 5. KOSUNEN, K. J., A simple method for measurement of angiotensin II in plasma, Scand J Clin Lab Invest 36: 467, 1976. 6. PAKARINEN, A., L. KOSKINEN, AND H. ADLERCREUTZ, Eval-
uation of radioimmunological methods for assay of plasma and urinary aldosterone, Scand J Clin Lab Invest 36: 331, 1976. 7. WILLIAMS, G. H , J. P. CAIN, R. G. DLUHY, AND R. H.
UNDERWOOD, Studies of the control of plasma aldosterone concentration in normal man. I. Response to posture, acute and chronic volume depletion and sodium loading, J Clin Invest Eil: 1731, 1972.
The Endocrine Society. Downloaded from press.endocrine.org by [${individualUser.displayName}] on 15 November 2015. at 02:26 For personal use only. No other uses without permission. . All rights reserved.
