Chondrocytes Expressing Intracellular Collagen Type II Enter the Cell Cycle and Co-Express Collagen Type I in Monolayer Culture Adel Tekari,1,2 Reto Luginbuehl,3 Willy Hofstetter,1 Rainer J. Egli1,3 1 Group for Bone Biology and Orthopaedic Research, Department Clinical Research, University of Bern, Bern, Switzerland, 2Graduate School for Cellular and Biomedical Sciences, University of Bern, Bern, Switzerland, 3RMS Foundation, Bettlach, Switzerland
Received 25 March 2014; accepted 17 June 2014 Published online 16 July 2014 in Wiley Online Library (wileyonlinelibrary.com). DOI 10.1002/jor.22690
ABSTRACT: For autologous chondrocyte transplantation, articular chondrocytes are harvested from cartilage tissue and expanded in vitro in monolayer culture. We aimed to characterize with a cellular resolution the synthesis of collagen type II (COL2) and collagen type I (COL1) during expansion in order to further understand why these cells lose the potential to form cartilage tissue when reintroduced into a microenvironment that supports chondrogenesis. During expansion for six passages, levels of transcripts encoding COL2 decreased to
Received 25 March 2014; accepted 17 June 2014 Published online 16 July 2014 in Wiley Online Library (wileyonlinelibrary.com). DOI 10.1002/jor.22690
ABSTRACT: For autologous chondrocyte transplantation, articular chondrocytes are harvested from cartilage tissue and expanded in vitro in monolayer culture. We aimed to characterize with a cellular resolution the synthesis of collagen type II (COL2) and collagen type I (COL1) during expansion in order to further understand why these cells lose the potential to form cartilage tissue when reintroduced into a microenvironment that supports chondrogenesis. During expansion for six passages, levels of transcripts encoding COL2 decreased to
