Sept., 1949]
DIAGNOSIS OF BUBONIC PLAGUE
Original DIAGNOSIS
Articles
of human bubonic
PLAGUE
BY AGGLUTINA-
TION TEST.* By G. PANJA and S. K. GUPTA (p lorn
.
Calcutta and Pathology, School of Tropical Medicine, Calcutta)
the
Department of Bacteriology
Bubonic plague sometimes prese: i
er>ideraic, laboratory diagnosis. During rjlaeue, many cases may be wrongly diagnos on the miSSed other hand, mild cases even by carefully eMmmation bacteriological
*n
q{ all the be examination may and their values indicated. There are two methods of diagnosis of a case of bubonic plague dun g ^fe firstly by puncture of the bubo, scopical and cultural examination of the tin ^
conducted.
At the
bacteriological
outset,
i
ip ?tioned ,
.
exan^1^tl^
obtained through puncture secondly, by blood culture.
tod
or
by
>
Buboes
and small in the
early .stage extremely difficult to get any fluid even
PL
often a. and it is
^
hminary Besides,
introduction of sterile sa it is really a torture to the natient, patlem, when bubo is punctured in a non- o ^ Still it is the method of choice a ^ nosis in early cases. Our practice stout needle and get even a drop o ^ absence of pus, eject the fluid on Diatinum t Plate, then spread out the same with P loop and finally incubate at room The material, diluted with sterile sa slope. inay as well be run down on a nutn g an(j We found it unnecessary to 1Tlclse culture,, scrape the material out of it. For blood^lLtur blood may either be put directly secment slope or into glucose broth fo the Plating. It is better to practis ^ as sometimes organisms ^ethods, -na_ few in the circulating blood. In of cases blood culture was the ^aeon but left out later, f that in many positive cases of gan pnimcture? blood culture was sterile, but not m y three case the reverse was the result. > _
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