Sept., 1949]

DIAGNOSIS OF BUBONIC PLAGUE

Original DIAGNOSIS

Articles

of human bubonic

PLAGUE

BY AGGLUTINA-

TION TEST.* By G. PANJA and S. K. GUPTA (p lorn

.

Calcutta and Pathology, School of Tropical Medicine, Calcutta)

the

Department of Bacteriology

Bubonic plague sometimes prese: i

er>ideraic, laboratory diagnosis. During rjlaeue, many cases may be wrongly diagnos on the miSSed other hand, mild cases even by carefully eMmmation bacteriological

*n

q{ all the be examination may and their values indicated. There are two methods of diagnosis of a case of bubonic plague dun g ^fe firstly by puncture of the bubo, scopical and cultural examination of the tin ^

conducted.

At the

bacteriological

outset,

i

ip ?tioned ,

.

exan^1^tl^

obtained through puncture secondly, by blood culture.

tod

or

by

>

Buboes

and small in the

early .stage extremely difficult to get any fluid even

PL

often a. and it is

^

hminary Besides,

introduction of sterile sa it is really a torture to the natient, patlem, when bubo is punctured in a non- o ^ Still it is the method of choice a ^ nosis in early cases. Our practice stout needle and get even a drop o ^ absence of pus, eject the fluid on Diatinum t Plate, then spread out the same with P loop and finally incubate at room The material, diluted with sterile sa slope. inay as well be run down on a nutn g an(j We found it unnecessary to 1Tlclse culture,, scrape the material out of it. For blood^lLtur blood may either be put directly secment slope or into glucose broth fo the Plating. It is better to practis ^ as sometimes organisms ^ethods, -na_ few in the circulating blood. In of cases blood culture was the ^aeon but left out later, f that in many positive cases of gan pnimcture? blood culture was sterile, but not m y three case the reverse was the result. > _

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Diagnosis of Human Bubonic Plague by Agglutination Test.

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