Article pubs.acs.org/JAFC
Development of an Immunochromatographic Strip Test for the Rapid Detection of Zearalenone in Corn Yaning Sun,†,§,∥ Xiaofei Hu,†,∥ Yong Zhang,§ Jifei Yang,† Fangyu Wang,† Yao Wang,† Ruiguang Deng,† and Gaiping Zhang*,†,# †
Key Laboratory of Animal Immunology of the Ministry of Agriculture, Henan Academy of Agricultural Sciences, Zhengzhou 450002, China § College of Veterinary Medicine, Gansu Agricultural University, Lanzhou 730070, China # College of Animal Science and Veterinary Medicine, Henan Agricultural University, Zhengzhou 450002, China ABSTRACT: A rapid immunochromatographic test strip has been developed for the detection of zearalenone (ZEN) residues in corn. For this purpose, a specific anti-ZEN monoclonal antibody (mAb), 4A3-F9, was obtained and identified. ZEN coupled to bovine serum albumin (BSA) via 1,4-butanediol diglycidyl ether was prepared as immunogen. The mAb showed low crossreactivity with five ZEN analogues. Using an antibody preparation with a titer of ≥1:5.12 × 105, the cross-reactivity (CR) of the anti-ZEN monoclonal antibody with four of the analogues was
Development of an Immunochromatographic Strip Test for the Rapid Detection of Zearalenone in Corn Yaning Sun,†,§,∥ Xiaofei Hu,†,∥ Yong Zhang,§ Jifei Yang,† Fangyu Wang,† Yao Wang,† Ruiguang Deng,† and Gaiping Zhang*,†,# †
Key Laboratory of Animal Immunology of the Ministry of Agriculture, Henan Academy of Agricultural Sciences, Zhengzhou 450002, China § College of Veterinary Medicine, Gansu Agricultural University, Lanzhou 730070, China # College of Animal Science and Veterinary Medicine, Henan Agricultural University, Zhengzhou 450002, China ABSTRACT: A rapid immunochromatographic test strip has been developed for the detection of zearalenone (ZEN) residues in corn. For this purpose, a specific anti-ZEN monoclonal antibody (mAb), 4A3-F9, was obtained and identified. ZEN coupled to bovine serum albumin (BSA) via 1,4-butanediol diglycidyl ether was prepared as immunogen. The mAb showed low crossreactivity with five ZEN analogues. Using an antibody preparation with a titer of ≥1:5.12 × 105, the cross-reactivity (CR) of the anti-ZEN monoclonal antibody with four of the analogues was
