Anal Bioanal Chem (2013) 405:9235–9249 DOI 10.1007/s00216-013-7325-z
RESEARCH PAPER
Development and validation of an UHPLC-LTQ-Orbitrap MS method for non-anthocyanin flavonoids quantification in Euterpe oleracea juice Aécio L. S. Dias & Eric Rozet & Yvan Larondelle & Philippe Hubert & Hervé Rogez & Joëlle Quetin-Leclercq
Received: 14 June 2013 / Revised: 20 August 2013 / Accepted: 26 August 2013 / Published online: 18 October 2013 # Springer-Verlag Berlin Heidelberg 2013
Abstract Euterpe oleracea fruits have gained much attention because of their phenolic constituents that have shown potential health benefits. The aim of this work was to quantify the major non-anthocyanin flavonoids (NAF) in the fruit juice by an accurate method coupling ultra-high pressure liquid chromatography with a linear ion trap-high resolution Orbitrap mass spectrometry system (UHPLC-LTQ- Orbitrap MS). Fruits were processed to juice, and then the juice was lyophilized and defatted. The residue was then extracted in the presence of methanol by sonication. The extraction time was optimized and recovery rates of the extraction were >90 %. The extracts were dried and solubilized again in 40 % MeOH, which showed the best compromise for MS detection. For the UHPLC quantification, a HSS C18 column (1.8 μm) was used with a gradient elution of methanol and water both with 0.1 % formic acid. Total error and accuracy profiles were used as validation criteria. Seven compounds and their isomers were successfully separated, including the major NAF. Calibration in the matrix was found to be more accurate than calibration without matrix. A. L. S. Dias : J. Quetin-Leclercq (*) Laboratoire de Pharmacognosie, LDRI, Université catholique de Louvain, Av. E. Mounier, 72, B1.72.03, 1200 Brussels, Belgium e-mail: [email protected] E. Rozet : P. Hubert Laboratoire de Chimie Analytique, Département de Pharmacie, CIRM, Université de Liège, CHU, B36, 4000 Liège, Belgium Y. Larondelle Institut des Sciences de la Vie, Université catholique de Louvain, Place Croix du Sud 2 bte L7.05.08, 1348 Louvain-la-Neuve, Belgium H. Rogez Faculdade de Engenharia de Alimentos, Universidade Federal do Pará & Centre for Agro-food Valorisation of Amazonian Bioactive Compounds (CVACBA), Av. Perimetral s/n, 66.095-780, Belém, PA, Brazil
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RESEARCH PAPER
Development and validation of an UHPLC-LTQ-Orbitrap MS method for non-anthocyanin flavonoids quantification in Euterpe oleracea juice Aécio L. S. Dias & Eric Rozet & Yvan Larondelle & Philippe Hubert & Hervé Rogez & Joëlle Quetin-Leclercq
Received: 14 June 2013 / Revised: 20 August 2013 / Accepted: 26 August 2013 / Published online: 18 October 2013 # Springer-Verlag Berlin Heidelberg 2013
Abstract Euterpe oleracea fruits have gained much attention because of their phenolic constituents that have shown potential health benefits. The aim of this work was to quantify the major non-anthocyanin flavonoids (NAF) in the fruit juice by an accurate method coupling ultra-high pressure liquid chromatography with a linear ion trap-high resolution Orbitrap mass spectrometry system (UHPLC-LTQ- Orbitrap MS). Fruits were processed to juice, and then the juice was lyophilized and defatted. The residue was then extracted in the presence of methanol by sonication. The extraction time was optimized and recovery rates of the extraction were >90 %. The extracts were dried and solubilized again in 40 % MeOH, which showed the best compromise for MS detection. For the UHPLC quantification, a HSS C18 column (1.8 μm) was used with a gradient elution of methanol and water both with 0.1 % formic acid. Total error and accuracy profiles were used as validation criteria. Seven compounds and their isomers were successfully separated, including the major NAF. Calibration in the matrix was found to be more accurate than calibration without matrix. A. L. S. Dias : J. Quetin-Leclercq (*) Laboratoire de Pharmacognosie, LDRI, Université catholique de Louvain, Av. E. Mounier, 72, B1.72.03, 1200 Brussels, Belgium e-mail: [email protected] E. Rozet : P. Hubert Laboratoire de Chimie Analytique, Département de Pharmacie, CIRM, Université de Liège, CHU, B36, 4000 Liège, Belgium Y. Larondelle Institut des Sciences de la Vie, Université catholique de Louvain, Place Croix du Sud 2 bte L7.05.08, 1348 Louvain-la-Neuve, Belgium H. Rogez Faculdade de Engenharia de Alimentos, Universidade Federal do Pará & Centre for Agro-food Valorisation of Amazonian Bioactive Compounds (CVACBA), Av. Perimetral s/n, 66.095-780, Belém, PA, Brazil
Trueness (
