Clin Chem Lab Med 2015; 53(3): 435–444

Sara Baldelli*, Dario Cattaneo, Luciana Giodini, Lorena Baietto, Giovanni Di Perri, Antonio D’Avolioa and Emilio Clementia

Development and validation of a HPLC-UV method for the quantification of antiepileptic drugs in dried plasma spots DOI 10.1515/cclm-2014-0472 Received May 2, 2014; accepted July 21, 2014; previously published online August 12, 2014

Abstract Background: Therapeutic drug monitoring (TDM) of antiepileptic drugs is widely used in clinical practice to optimise therapy, but it is limited by technical problems and cost considerations. The aim of the present study was: 1) to validate a chromatographic method for the concomitant determination of levetiracetam, lamotrigine, ethosuximide, felbamate, rufinamide, zonisamide and monohydroxycarbamazepine; 2) to develop it for dried plasma spot (DPS) assessing its reliability against the classical determination from plasma; and 3) test its clinical application. Methods: Extraction of plasma samples and DPS was done by simple precipitation. Chromatographic analysis was performed using high performance liquid chromatography with ultraviolet detection. After validation, both a Antonio D’Avolio and Emilio Clementi contributed equally to this work. *Corresponding author: Sara Baldelli, Chem.D., Unit of Clinical Pharmacology Luigi Sacco University Hospital via GB Grassi 74, 20157 Milano, Italy, Phone: +39 0250319619, Fax: +39 0250119646, E-mail: [email protected] Sara Baldelli and Dario Cattaneo: Unit of Clinical Pharmacology, Department of Biomedical and Clinical Sciences L. Sacco University Hospital, Università degli Studi di Milano, Milan, Italy Luciana Giodini: Experimental and Clinical Pharmacology Unit CRONational Cancer Institute, Aviano, Italy Lorena Baietto, Giovanni Di Perri and Antonio D’Avolio: Unit of Infectious Diseases, University of Turin, Department of Medical Sciences, Amedeo di Savoia Hospital, Turin, Italy Emilio Clementi: Unit of Clinical Pharmacology, Consiglio N]azionale delle Ricerche Institute of Neuroscience, Department of Biomedical and Clinical Sciences L. Sacco University Hospital, Università degli Studi di Milano, Milan, Italy; and Scientific Institute IRCCS Eugenio Medea, Bosisio Parini, Italy

methods were applied for the quantification of plasma samples from patients on antiepileptic therapy. Results: Mean inter- and intra-day accuracy and precision were  

Development and validation of a HPLC-UV method for the quantification of antiepileptic drugs in dried plasma spots.

Therapeutic drug monitoring (TDM) of antiepileptic drugs is widely used in clinical practice to optimise therapy, but it is limited by technical probl...
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