Anal Bioanal Chem DOI 10.1007/s00216-014-8385-4
RESEARCH PAPER
Development and co-validation of porcine insulin certified reference material by high-performance liquid chromatography– isotope dilution mass spectrometry Liqing Wu & Akiko Takatsu & Sang-Ryoul Park & Bin Yang & Huaxin Yang & Tomoya Kinumi & Jing Wang & Jiaming Bi & Yang Wang
Received: 9 October 2013 / Revised: 21 November 2014 / Accepted: 1 December 2014 # Springer-Verlag Berlin Heidelberg 2014
Abstract This article concerns the development and covalidation of a porcine insulin (pINS) certified reference material (CRM) produced by the National Institute of Metrology, People’s Republic of China. Each CRM unit contained about 15 mg of purified solid pINS. The moisture content, amount of ignition residue, molecular mass, and purity of the pINS were measured. Both high-performance liquid chromatography–isotope dilution mass spectrometry and a purity deduction method were used to determine the mass fraction of the pINS. Fifteen units were selected to study the between-bottle Published in the topical collection Reference Materials for Chemical Analysis with guest editors Hendrik Emons and Stephen A. Wise. Electronic supplementary material The online version of this article (doi:10.1007/s00216-014-8385-4) contains supplementary material, which is available to authorized users. L. Wu (*) : B. Yang : J. Wang Division of Medical and Biological Measurements, National Institute of Metrology, No. 18 Bei San Huan Dong Lu, Chaoyang District, Beijing 100013, China e-mail: [email protected] A. Takatsu : T. Kinumi National Metrology Institute of Japan, National Institute of Advanced Industrial Science and Technology (AIST), 1-1-1 Umezono C3-9, Tsukuba, Ibaraki, 305-8563, Japan S.
RESEARCH PAPER
Development and co-validation of porcine insulin certified reference material by high-performance liquid chromatography– isotope dilution mass spectrometry Liqing Wu & Akiko Takatsu & Sang-Ryoul Park & Bin Yang & Huaxin Yang & Tomoya Kinumi & Jing Wang & Jiaming Bi & Yang Wang
Received: 9 October 2013 / Revised: 21 November 2014 / Accepted: 1 December 2014 # Springer-Verlag Berlin Heidelberg 2014
Abstract This article concerns the development and covalidation of a porcine insulin (pINS) certified reference material (CRM) produced by the National Institute of Metrology, People’s Republic of China. Each CRM unit contained about 15 mg of purified solid pINS. The moisture content, amount of ignition residue, molecular mass, and purity of the pINS were measured. Both high-performance liquid chromatography–isotope dilution mass spectrometry and a purity deduction method were used to determine the mass fraction of the pINS. Fifteen units were selected to study the between-bottle Published in the topical collection Reference Materials for Chemical Analysis with guest editors Hendrik Emons and Stephen A. Wise. Electronic supplementary material The online version of this article (doi:10.1007/s00216-014-8385-4) contains supplementary material, which is available to authorized users. L. Wu (*) : B. Yang : J. Wang Division of Medical and Biological Measurements, National Institute of Metrology, No. 18 Bei San Huan Dong Lu, Chaoyang District, Beijing 100013, China e-mail: [email protected] A. Takatsu : T. Kinumi National Metrology Institute of Japan, National Institute of Advanced Industrial Science and Technology (AIST), 1-1-1 Umezono C3-9, Tsukuba, Ibaraki, 305-8563, Japan S.
