Vol. 58, No. 2
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, Feb. 1992, p. 713-716
0099-2240/92/020713-04$02.00/0 Copyright © 1992, American Society for Microbiology
Detection of Pediococcus spp. in Brewing Yeast by a Rapid Immunoassay MICHAEL WHITING,1 MICHAEL CRICHLOW,2 W. M. INGLEDEW 2 AND BARRY ZIOLA1* ImmunologylVirology Research Group, Department of Microbiology, College of Medicine,' and Department of Applied Microbiology and Food Science, College of Agriculture,2 University of Saskatchewan, Saskatoon, Saskatchewan, Canada 57N OWO Received 30 September 1991/Accepted 29 November 1991
A membrane immunofluorescent-antibody test was developed to detect diacetyl-producing Pediococcus contaminants in brewery pitching yeast (yeast [Saccharomyces cerevisiae] slurry collected for reinoculation). Centrifugations at 11 and 5,100 x g separate yeast cells from bacteria and concentrate the bacteria, respectively. Pelieted bacteria resuspended and trapped on a black membrane filter are reacted with monoclonal antibodies specific for cell surface antigens and then with fluorescein-conjugated indicator antibodies. Whether pitching yeast is contaminated with pediococci at 0.001% is determined in
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, Feb. 1992, p. 713-716
0099-2240/92/020713-04$02.00/0 Copyright © 1992, American Society for Microbiology
Detection of Pediococcus spp. in Brewing Yeast by a Rapid Immunoassay MICHAEL WHITING,1 MICHAEL CRICHLOW,2 W. M. INGLEDEW 2 AND BARRY ZIOLA1* ImmunologylVirology Research Group, Department of Microbiology, College of Medicine,' and Department of Applied Microbiology and Food Science, College of Agriculture,2 University of Saskatchewan, Saskatoon, Saskatchewan, Canada 57N OWO Received 30 September 1991/Accepted 29 November 1991
A membrane immunofluorescent-antibody test was developed to detect diacetyl-producing Pediococcus contaminants in brewery pitching yeast (yeast [Saccharomyces cerevisiae] slurry collected for reinoculation). Centrifugations at 11 and 5,100 x g separate yeast cells from bacteria and concentrate the bacteria, respectively. Pelieted bacteria resuspended and trapped on a black membrane filter are reacted with monoclonal antibodies specific for cell surface antigens and then with fluorescein-conjugated indicator antibodies. Whether pitching yeast is contaminated with pediococci at 0.001% is determined in
