lID 1991; 164 (October)
Concise Communications
disease manifestation, risk group, and immunologic defect. J Clin Immunol 1985;5: 130-8. II. Carini C, Mezzaroma I, Scano G, A'Amelio R, Matricardi P, Aiuti F. Characterization of specific immune complexes in Hl V-related disorders. Scand J Immunol 1987;26:21-8. 12. McHugh TM, Stites DP, Busch MP, Krawka JF, Stricker RB, Hollander H. Relation of circulating levels of human immunodeficiency virus (HIV) antigen, antibody to p24, and HIV-containing immune complexes. J Infect Dis 1988; I 58: 1088-91.
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13. Morrow W JW, Wharton M, Stricker RB, Levy J A. Circulating immune complexes in patients with acquired immune deficiency syndrome contains the AIDS-associated retrovirus. Clin Immunol Immunopathol 1986;40:515-24. 14. Kendall MG, Stuart A. The advanced theory of statistics. Vol. 2. New York: Hafner Publishing, 1967. 15. Roy S, Morrow WJW, Christian C, et al. Persistent immune complexes and abnormal CD4/CD8 ratios in HIV infection. J Acquir Immune Defic Syndr 1990;3: 134-8.
Detection of Human Immunodeficiency Virus DNA and RNA in Semen by the Polymerase Chain Reaction Center [or AIDS Research, Department of Medicine, Stanford University Medical Center, Stanford, California
Peripheral blood mononuclear cells (PBMC) and semen of23 men infected with human immunodeficiency virus (HIV) were examined for the presence of HIV DNA and RNA using the polymerase chain reaction (peR) and a nonisotopic detection assay. None of the men was receiving antiretroviral therapy at the time of collection. Semen samples were separated into cell-free seminal fluid, nonspermatozoal mononuclear cells (NSMC), and spermatozoa. All of the PBMC samples, 17 (74%) of23 NSMC samples, and none of the spermatozoal samples were positive for HIV gag gene DNA. Of 23 cell-free seminal fluid samples, 15 (65%) were positive for HIV gag gene RNA by PCR. Cell-free HIV RNA was more likely to be present in the semen of men with
Concise Communications
disease manifestation, risk group, and immunologic defect. J Clin Immunol 1985;5: 130-8. II. Carini C, Mezzaroma I, Scano G, A'Amelio R, Matricardi P, Aiuti F. Characterization of specific immune complexes in Hl V-related disorders. Scand J Immunol 1987;26:21-8. 12. McHugh TM, Stites DP, Busch MP, Krawka JF, Stricker RB, Hollander H. Relation of circulating levels of human immunodeficiency virus (HIV) antigen, antibody to p24, and HIV-containing immune complexes. J Infect Dis 1988; I 58: 1088-91.
769
13. Morrow W JW, Wharton M, Stricker RB, Levy J A. Circulating immune complexes in patients with acquired immune deficiency syndrome contains the AIDS-associated retrovirus. Clin Immunol Immunopathol 1986;40:515-24. 14. Kendall MG, Stuart A. The advanced theory of statistics. Vol. 2. New York: Hafner Publishing, 1967. 15. Roy S, Morrow WJW, Christian C, et al. Persistent immune complexes and abnormal CD4/CD8 ratios in HIV infection. J Acquir Immune Defic Syndr 1990;3: 134-8.
Detection of Human Immunodeficiency Virus DNA and RNA in Semen by the Polymerase Chain Reaction Center [or AIDS Research, Department of Medicine, Stanford University Medical Center, Stanford, California
Peripheral blood mononuclear cells (PBMC) and semen of23 men infected with human immunodeficiency virus (HIV) were examined for the presence of HIV DNA and RNA using the polymerase chain reaction (peR) and a nonisotopic detection assay. None of the men was receiving antiretroviral therapy at the time of collection. Semen samples were separated into cell-free seminal fluid, nonspermatozoal mononuclear cells (NSMC), and spermatozoa. All of the PBMC samples, 17 (74%) of23 NSMC samples, and none of the spermatozoal samples were positive for HIV gag gene DNA. Of 23 cell-free seminal fluid samples, 15 (65%) were positive for HIV gag gene RNA by PCR. Cell-free HIV RNA was more likely to be present in the semen of men with
