Accepted Manuscript Title: Delayed tooth replantation following root canal filling with calcium hydroxide and MTA: Histomorphometric study in rats Author: Jˆonatas Caldeira Esteves Heloisa Fonseca Mar˜ao Pedro Ivo dos Santos Silva Wilson Roberto Poi Sˆonia Regina Panzarini Alessandra Marcondes Aranega Eduardo Dias Ribeiro Celso Koogi Sonoda PII: DOI: Reference:
S0003-9969(15)00024-2 http://dx.doi.org/doi:10.1016/j.archoralbio.2015.02.002 AOB 3328
To appear in:
Archives of Oral Biology
Received date: Revised date: Accepted date:
15-9-2014 28-11-2014 2-2-2015
Please cite this article as: Esteves JC, Mar˜ao HF, Silva PIS, Poi WR, Panzarini SR, Aranega AM, Ribeiro ED, Sonoda CK, Delayed tooth replantation following root canal filling with calcium hydroxide and MTA: Histomorphometric study in rats, Archives of Oral Biology (2015), http://dx.doi.org/10.1016/j.archoralbio.2015.02.002 This is a PDF file of an unedited manuscript that has been accepted for publication. As a service to our customers we are providing this early version of the manuscript. The manuscript will undergo copyediting, typesetting, and review of the resulting proof before it is published in its final form. Please note that during the production process errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain.
1
Ac ce p
te
d
M
an
us
cr
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We examined the histological effect of MTA apical plug in tooth replantation For immature teeth a rigid MTA apical barrier makes easier an early endodontic treatment MTA plug decreases inflammatory resorption by retaining temporary dressing in the root canal
Page 1 of 30
2 Delayed tooth replantation following root canal filling with calcium hydroxide and MTA: Histomorphometric study in rats
Authors
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Jônatas Caldeira Esteves (DDS, MSc, PhD)#a Heloisa Fonseca Marão (DDS, MSc, PhD)b Pedro Ivo dos Santos Silva(DDS, MSc, PhD)c
cr
Wilson Roberto Poi (DDS, MSc, PhD)b Alessandra Marcondes Aranega(DDS, MSc, PhD)b Eduardo Dias Ribeiro (DDS, MSc, PhD)b
an
Celso Koogi Sonoda (DDS, MSc, PhD)b
us
Sônia Regina Panzarini (DDS, MSc, PhD)b
a
Federal University of Rio de Janeiro-UFRJ - Dental School – Rio de Janeiro-RJ –
Rio de Janeiro-RJ. CEP 21941-913
Univ Estadual Paulista – UNESP – Araçatuba Dental School – Araçatuba-SP– Brazil
d
b
M
Brazil. R. Prof Rodolpho Paulo Rocco 325 / 2o Andar – Ilha da Cidade Universitária –
c
te
Rua José Bonifácio, 1193 –Vila Mendonç – Zip Code 16015-050 – Araçatuba São Paulo Faculdades Integradas Aparício Carvalho – FIMCA – Porto Velho – Rondônia – Brazil
Ac ce p
Ruas das Araras, 241 – Eldorado – Porto Velho – Rondônia
_________________________________________________________________ #
Corresponding Author
R. Prof Rodolpho Paulo Rocco 325 / 2o Andar – Departamento de Clínica Odontológica - Ilha da Cidade Universitária – Rio de Janeiro-RJ. CEP 21941-913 Telephone/Fax: +55 21 3938-2033 Email [email protected]
Page 2 of 30
3 Abstract
Objective The purpose of this study was to evaluate, histomorphometrically, the repair process in
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rat teeth replanted after root canals were filled with calcium hydroxide (CH) and MTA.
Design
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Upper right incisors were extracted from 30 rats divided into three groups (n = 10). The
teeth were stored dry for 60 minutes, after which the pulp and periodontal ligament
us
(PDL) were removed and immersed in acidulated-phosphate sodium fluoride solution. In Group I, the root canals were filled with saline; in Group II, they were filled with CH; and in Group III, they were filled with CH, and the foramen was sealed with an
an
MTA plug. The teeth were replanted, and the animals were sacrificed after 60 days. The sections with teeth were removed for histological preparation (hematoxylin and eosin,
M
H&E). Characteristics of the PDL, cementum, dentin and alveolar bone, as well as the occurrence of inflammatory and replacement root resorption and apical sealing, were
te
Results
d
subjected to histological and morphometric analysis (P < 0.05).
Group I was the most affected by root resorption (mean = 67.05%). In Groups II and III,
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the resorption averaged 42.2% and 11.7%, respectively. Group III was less affected by inflammatory resorption and presented more areas of apical sealing by mineralized tissue (P
S0003-9969(15)00024-2 http://dx.doi.org/doi:10.1016/j.archoralbio.2015.02.002 AOB 3328
To appear in:
Archives of Oral Biology
Received date: Revised date: Accepted date:
15-9-2014 28-11-2014 2-2-2015
Please cite this article as: Esteves JC, Mar˜ao HF, Silva PIS, Poi WR, Panzarini SR, Aranega AM, Ribeiro ED, Sonoda CK, Delayed tooth replantation following root canal filling with calcium hydroxide and MTA: Histomorphometric study in rats, Archives of Oral Biology (2015), http://dx.doi.org/10.1016/j.archoralbio.2015.02.002 This is a PDF file of an unedited manuscript that has been accepted for publication. As a service to our customers we are providing this early version of the manuscript. The manuscript will undergo copyediting, typesetting, and review of the resulting proof before it is published in its final form. Please note that during the production process errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain.
1
Ac ce p
te
d
M
an
us
cr
ip t
We examined the histological effect of MTA apical plug in tooth replantation For immature teeth a rigid MTA apical barrier makes easier an early endodontic treatment MTA plug decreases inflammatory resorption by retaining temporary dressing in the root canal
Page 1 of 30
2 Delayed tooth replantation following root canal filling with calcium hydroxide and MTA: Histomorphometric study in rats
Authors
ip t
Jônatas Caldeira Esteves (DDS, MSc, PhD)#a Heloisa Fonseca Marão (DDS, MSc, PhD)b Pedro Ivo dos Santos Silva(DDS, MSc, PhD)c
cr
Wilson Roberto Poi (DDS, MSc, PhD)b Alessandra Marcondes Aranega(DDS, MSc, PhD)b Eduardo Dias Ribeiro (DDS, MSc, PhD)b
an
Celso Koogi Sonoda (DDS, MSc, PhD)b
us
Sônia Regina Panzarini (DDS, MSc, PhD)b
a
Federal University of Rio de Janeiro-UFRJ - Dental School – Rio de Janeiro-RJ –
Rio de Janeiro-RJ. CEP 21941-913
Univ Estadual Paulista – UNESP – Araçatuba Dental School – Araçatuba-SP– Brazil
d
b
M
Brazil. R. Prof Rodolpho Paulo Rocco 325 / 2o Andar – Ilha da Cidade Universitária –
c
te
Rua José Bonifácio, 1193 –Vila Mendonç – Zip Code 16015-050 – Araçatuba São Paulo Faculdades Integradas Aparício Carvalho – FIMCA – Porto Velho – Rondônia – Brazil
Ac ce p
Ruas das Araras, 241 – Eldorado – Porto Velho – Rondônia
_________________________________________________________________ #
Corresponding Author
R. Prof Rodolpho Paulo Rocco 325 / 2o Andar – Departamento de Clínica Odontológica - Ilha da Cidade Universitária – Rio de Janeiro-RJ. CEP 21941-913 Telephone/Fax: +55 21 3938-2033 Email [email protected]
Page 2 of 30
3 Abstract
Objective The purpose of this study was to evaluate, histomorphometrically, the repair process in
ip t
rat teeth replanted after root canals were filled with calcium hydroxide (CH) and MTA.
Design
cr
Upper right incisors were extracted from 30 rats divided into three groups (n = 10). The
teeth were stored dry for 60 minutes, after which the pulp and periodontal ligament
us
(PDL) were removed and immersed in acidulated-phosphate sodium fluoride solution. In Group I, the root canals were filled with saline; in Group II, they were filled with CH; and in Group III, they were filled with CH, and the foramen was sealed with an
an
MTA plug. The teeth were replanted, and the animals were sacrificed after 60 days. The sections with teeth were removed for histological preparation (hematoxylin and eosin,
M
H&E). Characteristics of the PDL, cementum, dentin and alveolar bone, as well as the occurrence of inflammatory and replacement root resorption and apical sealing, were
te
Results
d
subjected to histological and morphometric analysis (P < 0.05).
Group I was the most affected by root resorption (mean = 67.05%). In Groups II and III,
Ac ce p
the resorption averaged 42.2% and 11.7%, respectively. Group III was less affected by inflammatory resorption and presented more areas of apical sealing by mineralized tissue (P
