Supplementary online material of Beneficial Microbes DOI: http://dx.doi.org/10.3920/BM2014.0046.
Degenerate PCR primers for detecting putative priming glycosyltransferase genes in Bifidobacterium strains C. Hidalgo-Cantabrana, I. Ordoñez, P. Ruas-Madiedo and A. Margolles Figure S1. Nucleotide sequence alignment for the cpsD gene of 7 different Bifidobacterium species. From top to bottom: BL0237 (accession no. NP_695447) from B. longum subsp. longum NCC2705, BAD_1389 (YP_910252) from B. adolescentis ATCC 15703, BIFANG_03177 (ZP_04448174) from B. angulatum DSM 20098, BLIG_00247 (ZP_04665465) from B. longum subsp. infantis CCUG 52486, BLA_0595 (YP_002469468) from B. animalis subsp. lactis AD011, BIFGAL_04272 (ZP_05966491) from B. gallicum DSM 20093 and BIFPSEUDO_03247 (ZP_03742673) from B. pseudocatenulatum DSM 20438. The grey shadows indicate the primer sets (F1R2 and F4R6) that yielded the best results. B. B. B. B. B. B. B.
longum adolescentis angulatum infantis lactis gallicum pseudocatenulatum
--------------------------TTGGAGAGAAGGATGT-CCCAAAGCGGTAGCGCT --------------------------------------ATGG-CTTACAGTGGCAATAGT TTGAAGGTTGCCCCCTCCTATGCGCTTTGGGGGGAGAGCCGG-CAGGAGAAAGAAGACAT --CTGAGCAGTAACGGTCAGCCGTCTACACGCAGCAGACAGG-CCAATAGCGGTGACCGA --------------------------------ATGGCACAGG-CGAAAAG-------CGA --------------------------------ATGTCACAGG-CCAAGACGG-----CTA ----ATGCAAGGCGGTTTCCGCAGCTCTGAGCAGGGTTCGGATCAAGTAGTGAGAACCCA . * * . . :
33 21 59 57 20 22 56
B. B. B. B. B. B. B.
longum adolescentis angulatum infantis lactis gallicum pseudocatenulatum
GAAACGA-------------ATCTGAGTGAAACCTCCCGTAAACGGGTGCTTAGCGTCGC GGGGAGA-------------AACTGAGCGAGACCTCACGCAGGCATGTGCTCAGCGTTGC GACGCAAGCCAATAGCACCGCACTGAAAGACACATCGTGGCAGCAGCTGGTGAATGTTGC ACCACCG----------CTCAGCTCAGTGATATCCGCCAAAAGCATCTCAGCCACGTGCT TGCTCAG-------------CTCGCTGAGGAAGTGGACGTCCGCTCCAGCAGGGTGCTGC AACTCGC-------------TGACCTTACGCAACAGAGGTCAGCGCATACAT-----TGC GGAAATCCAT---------ATCGTCTCTTCTAACTCTCGTCACCAGGATCAGAAGCAGGA . : * . . * :
80 68 119 107 67 64 107
B. B. B. B. B. B. B.
longum adolescentis angulatum infantis lactis gallicum pseudocatenulatum
TTATTGGCGTTTTTTCGTCAATGCTCTTTTGATT-----------CTGTGCGACATGGCT CTACTGGCGTTTCTTCGTCAACGCCCTGTTGGTG-----------TTGTGCGATATGGCT GTATTGGAGGTTCTTCGTCAATGCGGCATTGACT-----------GTTTGCGATATGGCC GTACTGGCGATTCTTCGTCAATCTCGCCCTCATG-----------CTTATCGACGCCGCC G--ATGGCGTTTCGTCATGAACCTGGCACTCATG-----------CTGTTCGATGCGTTG G--GTGGCGATTCTCGTTGATTCTGCTGCTGATG-----------GTGCTTGATTTTCTT GCGACATCAGTCTCAAGACAACAGCCCTCAGTTTGAGCCGATGATGTTTTCGAACACGCC . .. * : *: : * **
129 117 168 156 114 111 167
B. B. B. B. B. B. B.
longum adolescentis angulatum infantis lactis gallicum pseudocatenulatum
TCATTT--------------ATTGGCGCTTCGGCAATCATATATGTGTGG---CAGGGCG GCCTTC--------------ATCATCGCTTCGGCGATCGTATACGTGGTG---CGGAATA GCGTTT--------------GTGCTCGCTTCGGTGATTGTGCTTATGGCG---CGCAATG ATGTAC--------------ATCATCGCCGGAGCCACGGTGCTCAATCTG---CGTCATG GTCTAC--------------TTCGCTACCAGCATGATTGTGCTGGTCGTA---TCGCATG GTGTAT--------------GCCG-TGGTGTCAT--TCATTCTTATCGAG---GACTTTG ATTTTCCGTTGGTGAAGATGGAAAGCGCAAGGGCAGGCGGAATCAGGTTGTGCCCGCATG *: . . . : . . .
172 160 211 199 157 151 227
B. B. B. B. B. B. B.
longum adolescentis angulatum infantis lactis gallicum pseudocatenulatum
GGGAAGC---GTTGTATTCTTCCCG----------------------------TTTC--CCGGTGA---TTTATATTCCACGCG----------------------------TTTC--AGGCGGC---GCTGTATTCCGCTCG----------------------------TTTC--AGGCAACGCCGTTCTATTCGAGCAG----------------------------ATTC--GCACGAA---TCTAGTGACGACTCG----------------------------TTTG--ACGAGCT---CAGGCAGCCGGTGAT----------------------------TTTC--GCGTTATTCTTTTGTCGTCTGCTTGGTGATGGTCGATCTTGCGGTCATGCTGATTTCGCT . * :**
198 186 237 228 183 177 287
B. B. B. B. B. B. B.
longum adolescentis angulatum infantis lactis gallicum pseudocatenulatum
-----------------------------CATTT------CCATATTGGTTTGTGGC-------------------------------CACTT------CCATATTGCTTTGTGGG-------------------------------CATTT------CCATATCAATCTGGCCG-------------------------------AATTT------CACGATTGACTCCACCG-------------------------------CAACTGAATCTGCCGATAGACCCGTGGC-------------------------------CGTTTTGATGTGCCGATGAACGCGTTCT--ATCCATCAGTCTTTGGGCCAACGATGCCGCATATAATGCCATCAATGGGGCCATGCCGTT .. * . ** .
220 208 259 250 211 205 347
B. B. B. B. B. B. B.
longum adolescentis angulatum infantis lactis gallicum pseudocatenulatum
-----TGTATGCGCTTGCTTGCTCGTTGATTTGGATCTACTGCCTGCATAGTGTTGGCGT -----CGTATATGCTTGTTGGGTCGCTGATTTGGATATATTGCCTGCACAGCGTAGGCGT -----TGTATGTGATTGTGTGCGCGCTGATTTGGGCGCTGTGCATGCACAGCGTGGGCGT -----TTTACTTTATGATTGTGGCCTGCTTCTGGGTGTGGTCGCTCTATGTCGCCGGCAT -----TGTATTCCGCTGCCTGCGCGGCGATTTACGTCTACTGCATGCATGTGGCGGGGGT -----GGTACATCGTTATTTGCTCGCTGATTTATGTGTGCTGCCTCAAGATGTCTGGCGT CTGGCTGTTCCTCATCTGCTTCAGCCTCATCTGGGTGTTGAGCCTCACCTTTGCCGGAAC *: :* *. . : .* . ** .
275 263 314 305 266 260 407
B. B. B. B. B. B. B.
longum adolescentis angulatum infantis lactis gallicum pseudocatenulatum
GTACCACCGTCATGTGATGAGTGACGGTTACCAGTTGAACGCGCTACTGGTCAAAGGCAC GTACCACCGTCATGTGATGGGTGATGGTTACCAGCTCAATGCGTTCCTGATCAAGGGCGC GTATCACCGGCATGTGATGGGTGACGGCTACCAGCTGAACCTGCTGCTGTTCAAGGGCGC TTATCATCGCCATGTTATGGGTGACGGTTACCAGCTCAATGTGCTGCTGGTCAAAGGCAT GTACCACCGGCATGTGTTCGCCGACGGATACCAGCTCAACATGTTGCTACTCAAGGGTGG GTATCATCGCCATGTGCTTGGTGACGGTTATGAGCTCAACGTGCGTTTGCTCAAAG-CGG GTATCATCGCCATGTGATGGCGGAAGGCTACGAGCTGTATGCGAAAATCATCAACGCGTC ** ** ** ***** * . ** ** ** ** * :* * * **** *
335 323 374 365 326 319 467
B. B. B. B. B. B. B.
longum adolescentis angulatum infantis lactis gallicum pseudocatenulatum
GTTTTTCACGGGATTGATGATT-TGTGCAGTTGATTCTATTCTCAACGTTTATATACCTT GCTGTTCGCGGGTTTGATGGTT-TGCGCGTTGAATTCCATTCTCAACGTGTATGTGCCTC GGTGCTCGCCGGCATTATGGTG-TGCGCGGTCGATTATATTCTCAACTCGTATATTTCCC GGTCGTGTGCTGGGTGGTGCAG-TGCGCGTTCAGCTACTTCTTCAATCTGGGTCTGACCC CTTCATTGCCTGGGTGGCGATT-TGCGCGCTGAATTTCATCATTCAGTGCAACATATTCT CCGTAATGGATTTCTGCCTCGTGTGCTCGCTCAATTATGTGCTCGAATCTAAAATCGGCA GCTGCTCACCATCATCACTTAC-TGCTCGCTTGCTTTTATTTTCAATCTTTCCTTGCCGA : * ** *. * . * * * * *
394 382 433 424 385 379 526
B. B. B. B. B. B. B.
longum adolescentis angulatum infantis lactis gallicum pseudocatenulatum
TGCTGCATACCGGCTTGGTGTTGCTGTGCGCATTCCTGATTACCATGCTGGAGCGTTTGA TGGTGACCACTGGATTGGCGCTGCTGTGCGCGTTGCTGGTCACCATTGTCGAACGTTTCA TGCTTTCCACGGCGCTGATCATGGTGTGCGCGCTGCTGATCACCATGGCTGAGCGGTTTG TTACCTCGCTCTGCTTGGTGGTGATTGCCGGATGGTTGCTCACCATGGTGGAACGCATTG TCTACATGGTCTGCCTCACCTCGTTGATTGTGTGGGTGGCGGAAATGATCGTTCGCTTCA TCCGCATGGTCGTGCTGACGTCGCTGTTCGCGTGGATCGCCACGATGATTATGCGTATGA GAACCGCGCTTATCGTCGCCCCGATCATTGCATTCTTCCTAGAAGTCATCGCCCGTTGGC * . * * * . * .. .* . ** :
454 442 493 484 445 439 586
F1
B. B. B. B. B. B. B.
longum adolescentis angulatum infantis lactis gallicum pseudocatenulatum
TTATCCGCCAGTTTGTGCTGTCCAGCCGTGAAAAGGGCGCATATTCGTATGGCACCGTGG TCATTCGTCAGTGCGTGCTGTTCAGCCGTGAGAAGGGCGCGTATTCGTATGGCACCGTGG TGATTCGCCGCCTGGTGCTGTTCGGCCGTGAGAAGGGCGCCTACTCGTATGCCACGGTGG CCAGCCGTGCATTCATTACGCGTAGTCGTCGCAAGGGCGCCTACGCTTACGGCACGGTGA TCGCGCGCGTCATTCTCACCAGTAAACATCGCAAAGGGGAGTATTCGTACCCGACCGTCA TTGTGCGCTTTATTGTGACGCGCCGCCGGCGTAAAGGTGCCTATACCTACCCGACAGTAA AGATGCGTCAGTGGCAGCATCGCAGCCGTCAGAAGGGCGCCTGTAAGTATAAGACCGTGG . ** : . . *. . **.** *. *. . ** ** ** .
514 502 553 544 505 499 646
B. B. B. B. B. B. B.
longum adolescentis angulatum infantis lactis gallicum pseudocatenulatum
TGGTTGGTTCCCCTGATGGTATTGAACATGCTTTGGAATTTCTGAGCCAGAAGCGTCAAC TGGTCGGCTCGCTGGAGGGCATTGAGCATGCCTTGCAGTTTTTGAGCCGGAAGAGCCAGC TTGTTGGCTCGTGCGAGGGCATTGAGCATGCGTTGCGTTTTCTGAGCCAGAAGCAGCAGT TCATCGGTTCCCCGCACGGCATTGGTCGCACGCTAAAATTCCTCGGTCAGCGTCGGCAAC TCGTCGGTTCCCCCGAGGGTATTGGGCAGATTCTCAAGTTCCTCAATCAACGCAGACAGC TTGTCGGGTCTCCGGAGGGTATTGGGCAGGCGCTCAAATTCATTAATAATCGCAAGCAAC TGATTGGTTCTTCCGACGGTATCAATTGTGCGTTGCGCACCATGCGGGACTACTCCAATT * .* ** ** * ** ** .. . . * . : * . . . .*
574 562 613 604 565 559 706
B. B. B. B. B. B. B.
longum adolescentis angulatum infantis lactis gallicum pseudocatenulatum
TGAATTATCATGCTATTGCGGTGTGCCCGATTGGTTTGAACAAGCGGACTGGGCTCGTGG TGAACTACCATGCCATTGCGGTGTGCCCGATTGGGCTGGATGCGCAGACCGGCTATGTAA TGAATTACCGCACGGTGGCGGTGTGCCCGATTGGCTTGAATGCGCAGACCGGGCATATTG TCAACTACCGCCCGGTGGCAGTATGCCCGATTCATCTCAATCCCGATACCGGATTGATTG TCAATTACAAGCCAATTCGCCTGTGCCCGATTCGGGTCAATAGCGAGACGGGGCTCATTG TCAATTACAAGGCCATTGCCGTGTGCCCGATCCGCGTGAACCGCAAGACGGGTTTGATTG GGGGGTATTCGCCGATTGCCGTGTGTCCGATCGAAGCTGACGACAATGAGAAAGACGCTT .. ** * .* *.** ***** . .* . .. .. : .
634 622 673 664 625 619 766
B. B. B. B. B. B. B.
longum adolescentis angulatum infantis lactis gallicum pseudocatenulatum
AGGCCGTTGTCGCACCTGCTGAT-TTCAAGGATCGAATCCGCAAGATCAG-TGGTAGGGA AGGCCGTTGATGTTACCGCTGAA-TTTAAGCAGCGGATCCGTGAGATTTG-TGGTAGGGA AGGCTGCCAGCGCACCCGAGGAG-TTTATGCAGGAGATTCGCCGGATTGG-TGGTAAGGA AACAGAGCACCGACCATGAGACA-CTCCGTGAGGAGATGCAAAAGAACGA-GGGCTGCCA AGGCGGATCCGAACACCGAATTG-CTCAGCAAGGAAGTGCATGAGTCATGGAATTCCCCG AGGCTGATGACGATTACGAGGCG-CTCAAGACACAGATGAAAG-GCCATGGTGGCCACGG ATGTGGTCACGTCGTTCGAGCCTGATCCTTCCATTGAAGGTGCCGACAAG-CTGAAGGTG * . *. * . . ..: * . .
692 680 731 722 684 677 825
B. B. B. B. B. B. B.
longum adolescentis angulatum infantis lactis gallicum pseudocatenulatum
CATCGTCACATTGCCGTATTGCGG---GAACTTCGCCGAACGTGCCGCGTCCATGGGCGT TATCGCCACGTTGCCATATTGCAA---GAATTTTGCCGAACGCGCCGCGTCCATGGGCGT GATTCCCGTGCTGGCGTATGGCGG---CGATCTGGCCGAACGTGCTGTGGCCATGGGCGC GCTGCCCGTACTCGAGTACAGCGATCATAATCTTGCTGAACAGATTATTGACATGAACGC ATTGATGTGATTGA-ATATGGACC---CCAGCTGGCTGGACGGATTGCGGAAACCGGCGC CATTATGCCGGTGCGCTATGGCGA---GAACATGGGGGAGCGGTTCGCCCAGCTTGGCGC ATTCCGTTCAATGCCGCTTTCCCG------------CGTACGTG-CGAAAGCTTGGGTGC * . * : . * .*. . .. *
749 737 788 782 740 734 872
B. B. B. B. B. B. B.
longum adolescentis angulatum infantis lactis gallicum pseudocatenulatum
GCAGACGGTGATGGTTACGGATGTTATGCAGCGCTTCTCGGATAATTTCAACACGTTTGT GCAGACGGTGATGGTTACTGATGTGATGCACCGGTTCTCGGACAATTTCAACACGTTTGT ACAAACCGTGATGGTGACGGATGTGCTGCAGCGTTTCTCCGACAATTTCAATACGTTTGC GCAGACCGTTATGGTGGCTGACGAGCTGCATCGCTATTCGGATAATTACGATATCTTCTC ACAGACGGTGATGGTCACCGATGTGCTCGACCGATTCTCCGACAATCTGAGCACTTTTGC GCAGACGATCATGGTTACCGATGTGCTCGACCGGTTCTCCGACAACCTCAACATGTTCTC GCAGGAAGTGTATGTTGCCGACGTGCTGTCCCGCGATTCCGAAATGCTGCATGGCATTTC .**... .* :: ** .* ** *: .* . ** : ** ** *: : . . :*
809 797 848 842 800 794 932
B. B. B. B. B. B. B.
longum adolescentis angulatum infantis lactis gallicum pseudocatenulatum
GCTGAACATGGAAACCATGAATCTGGAGATTGCGGTGGTCACTTCTGCAGTGGACGTGAG GCTGGACGTGGAGGCCATGAACTTGGAAGTGGCGTTGATTACCTCCGCGGTGGATGTGAG GTTGAGTCTTGAATCCATGAACCTGGAGATTGCGCTGGTTACCTCTGCCGCTGATGCGAG CGTGCGCATGGAATCGTTCGGTCTGGAGATTGCCGTGCTGGCTTCCGCCGCTGACACTTC TTTGCAGATGGAATCCGTGAATCTTGAGATTGCGCTGATCACCTCTGCGCTTGACATTGC GCTGCAAATGGAAGCCATGAACCTTGAGGTCGCGCTCGTCACGTCCGCAGTGGACATCGC CCTTGCGGTCGAGTCGCTTGGCATGGAGCTTGCGTTGGCTGTTTCCTTGGCTGACGTGAG * * **. * * .. * **. * ** * . ** ** .
869 857 908 902 860 854 992
B. B. B. B. B. B. B.
longum adolescentis angulatum infantis lactis gallicum pseudocatenulatum
TGGTCATGAGACCAACATCCGTGTGATTCAGGGTACGACGGTGATGACGATCAGCCTACC CGGCCATGAGACCAACATCCGTGTGATTCAGGGTTCCACGGTGATGACCATCAGCCTGCC CGGCCATGAGACGAATGTGCGTGTGATTCATGGCGCTACGGTGATGACCATCTGTCTGTC CAACCATGAGATTCAGGTTCGATCCATACAGGGCACCACGATTATCACGCAACGTCTTGC CGAGCATGCCACGCAGATGCGCAACATTCAGGGTATGAGCATCATGACGATCAGTCTGCC GGGCCATGCCACGCGCATCCAAAACCGTAACGGCCTGACGATCATGACGATTTACTTGCC CGGCCACCGCCTGTACTTGCGTAATACGGCCGAACAGCCGGTATTGCTGGCCAGTTTGCC .. ** . . * *. . . *. . .* :* . . * *
929 917 968 962 920 914 1052
B. B. B. B. B. B. B.
longum adolescentis angulatum infantis lactis gallicum pseudocatenulatum
GCAGTATTCGCCATGGGATATGTGCAAAAAACGTGTTTTTGATGTGCTGGTTTCGCTGTT GCAGTATTCGCCGTGGGCCATGTTCAAGAAGCGTGTGTTCGATATTGTGGTGTCGCTGAT GCAGTATTCGCCGGCTGTGATGCTGCAGAAGCGTGTGTTCGACGTTGTGGTCTCGCTGCT GCAGTACACGCCAGCCCGCCGTATGGCTAAGCGCCTGTTTGACTTGGTGATTTCTTCGCT TCAGTATTCCCCCTCCGCACAGATTAAGAAACGCGTGTTCGATCTCGTGGTGTCGACGAT GCAGTATTCGCCATATGAGCAAATCAAGAAGCGCATCTTCGACATTGTGGTTTCGAGTGT GCAGTATACGAATACGACGTACGTGATTAAACGCCTGCTGGATATCGTTGGTTCGGCTTT ***** :* .. **.** * * ** * * . ** *
989 977 1028 1022 980 974 1112
B. B. B. B. B. B. B.
longum adolescentis angulatum infantis lactis gallicum pseudocatenulatum
TGCCGTGATTGTTGCTGCGATTGTTACCGTTCCTATTGCGATTGCTATCAAGCTTACCGA GGCATTGGTTGGCACCGCGATTATTACCATCCCGGTGGCCATTGCCATCAAGGTGACCGA CGCATTGGTGGGTACGGCCATCATCACTGTTCCGGTGGCCATTGCCATCAAAGTGACCGA AGCGATCATCTGTTCGTTGATTATCACCATCCCGGTGGCGATCGCCATCAAAGTCACGGA TGCGATCATTCTGAGCTCGCCGATCATGCTGATCGTGGCGTTGGCGATCAAGATTGAAGA GGCCCTTGTGGTGTCGTCGCCAATCATGGCCATCGTGGCTTTGGCCATCAAGATGGAAGA CGCGCTGCTGATTTCCTCGCCGCTGATGTTGGGTACGGCGATCGCCATCAAGCTTGATGA ** * * . * * . ** :* ** *****. * .. **
1049 1037 1088 1082 1040 1034 1172
B. B. B. B. B. B. B.
longum adolescentis angulatum infantis lactis gallicum pseudocatenulatum
CGGCGGTCCTATTTTCTACACGCAGACTCGAGTTGGGCGCCGTGACAAGCCGTTTAAGAT TAGAGGGCCTATTTTCTACACTCAGACCCGTGTGGGTCGCCGTGGCAAGCCGTTCAAGAT TGGCGGGCCGATCTTCTATACGCAGACCCGTGTAGGCCGCCGTGGCAAGCCGTTCAAGAT CGGCGGACCGGTGTTCTACAAGCAGACCCGTATGGGCCTGCGCGGCAAGTCGTTCCAGAT CGGCGGTCCGGTGTTCTATAAGCAGGAGCGTATCGGCTTGCGCGGCAAGCCGTTCTATAT TGGCGGCCCAGTGTTCTATAAGCAGGAGCGCATCGGATTGCGCGGCAAGCCGTTTTATAT CGGTGGTTCGATTTTCTTCTCGCAGCAGCGTATCGGTTTGCATGGCAAGCCGTTTACCAT .* ** * .* ****: :. *** . ** .* ** *. *.**** **** . **
1109 1097 1148 1142 1100 1094 1232
B. B. B. B. B. B. B.
longum adolescentis angulatum infantis lactis gallicum pseudocatenulatum
GATCAAGTTCCGTTCTATGGTGGTGGATGCGGACAAGATGAAGGCAGAACTGGCGAAAGA GATTAAGTTCCGTTCCATGGTGGTGAACGCCGACAAGATGAAGTCGGAACTGGCGGAGCA GATTAAGTTCCGTTCCATGGTGGTGAATGCCGACAAGATGAAGGCTGAGCTGGCTGCGGA GTACAAGTTCCGTTCGATGGTGGTGAACGCCGACGCGCTCAAGAAGGAGCTCGCAAAAGA CCATAAGTTCCGTTCGATGCGTGTGGATGCCGACGCACACCTGAAAGAGGTATTGGCTGC CCACAAGTTCAGGTCGATGTATGTCAATGCGGACGCGCGCCTTGAGGAAGTGCTCAAAGA GTATAAATTCCGTTCCATGGTCACCAATGCGGAAGAATTGAAGAAGAAACTTGCCGAAGA : **.***.* ** *** . .* ** **.... .: . .*. * .. .
1169 1157 1208 1202 1160 1154 1292
F4
R2
B. B. B. B. B. B. B.
longum adolescentis angulatum infantis lactis gallicum pseudocatenulatum
AACCGGTCAGGATGGTCGTTTTATCTTCAAGATGAAGGACGATCCGCGAGTCACCAAGGT GACCGGGCAAAAGGGACGGTTCATCTTCAAGATGAAGAACGATCCCAGAGTCACTACAGT GACTGGGCAGAAGGGTCGTTTCATTTTCAAGATGAAGGACGATCCGCGAGTCACCAAGGT AACCGGCCAGACCGACCGCTTCATCTTCAAGATGAAGAACGACCCACGCATCACCAAGGT GCAAGGGCAGGAATTGGGAGCCCGGTACAAGCTCAAGCACGACCCGCGCATCACGAAGGT ACAAGGTCAGGAACTTGGCGCCCGATACAAGCTCAAGCATGATCCGCGTATCACCAAGGT AAATGGACAGACCGATCGTTTCATCTTCAAAATGAAGGATGATCCGAGAATTACGAAGGT ... ** **... * . *:***..* *** * ** ** .* .* ** *..**
1229 1217 1268 1262 1220 1214 1352
B. B. B. B. B. B. B.
longum adolescentis angulatum infantis lactis gallicum pseudocatenulatum
GGGCAAGTTTATCCGTAAGTTCTCCATTGACGAGTTTCCGCAGTTCCTGAACGTGTTGAA GGGCCGGTTCATCCGCAAGTTCTCCATCGACGAGTTCCCGCAGTTCCTGAACGTGTTGAG GGGCAAGTTTATCCGCAAGTTCTCCATCGATGAATTCCCCCAGTTCCTCAATGTGCTCAA GGGGCATTTCATTCGCCGCTTCTCCATTGACGAGTTGCCGCAGTTCCTCAACGTGTGGAT GGGTAACTTCATTCGCAAGACGAGTCTCGACGAGCTTCCGCAGTTCTTCGATGTGTTTAT CGGCGCATTCATCCGCAAGACGAGCCTGGACGAGTTGCCGCAGTTCTTTGACTCGTTCCG CGGTCGTTTTATTCGTAAGACTTCGTTGGATGAATTCCCCCAGTTCTTCAACGTTTTGAA ** ** ** ** .. : : * ** **. * ** ****** * .* .
1289 1277 1328 1322 1280 1274 1412
B. B. B. B. B. B. B.
longum adolescentis angulatum infantis lactis gallicum pseudocatenulatum
GGGCGATATGAGCGTGGTGGGCCCGCGTCCGCCGTTACCTGAGGAGGTGGCTCAATACAA GGGCGATATGAGCGTGGTGGGCCCGCGCCCGCCGCTGCCTGAGGAAGTGGCCCAGTACAA GGGAGATATGAGCGTGGTGGGCCCGCGCCCGCCGCTGCCCGAGGAAGTGGCTCAATACAA GGGGGATATGTCCGTAGTGGGGCCGCGCCCGCCGCTGCCTGAGGAATATGCCCGTTACAA GGGTTCGATGAGTGTGGTGGGGCCGCGCCCGCAACGTTCATTCGAGGTGGCCGAGTATGA GGGCGCCATGAGTGTGGTGGGTCCACGACCACAGCGCGATTTTGAAGTGCGTGAATATGA GGGCGACATGTCTTTGGTTGGTCCTCGCCCGGCTTTGCCGGAAGAGGTTGCTCGCTATGG *** . ***: *.** ** ** ** **. . . : **. : . ** ..
1349 1337 1388 1382 1340 1334 1472
B. B. B. B. B. B. B.
longum adolescentis angulatum infantis lactis gallicum pseudocatenulatum
CCAGACGTATGCCACTCGTATGCTGGTGAAGCCTGGTATTACCGGCCCGTGGCAGGTGTC CCAGACGTATGCCACTCGCATGCTGGTGAAGCCTGGTATCACCGGTCCGTGGCAGGTATC CCAAACATACGCTACCCGTATGCTGGTCAAGCCCGGTATTACCGGCCCCTGGCAGGTGTC CCGTCTGTACGCCACCCGCATGCTGGTCAAGCCCGGCATCACCGGTCCGTGGCAAGTGTC CCCGGTCTATGCGACACGTCTGTTGGTCAAGCCCGGCATCACTGGTCCCTGGCAGGTGTC CCGTGTCTATGCTTCGCGTTTGCTCGTCAAGCCCGGTATTACCGGTCCTTGGCAGGTCTC TTCCCTTTATTCCGCGCGACTGCTGGTTAAGCCTGGTATTACCGGTCCGTGGCAGGTTTC ** * * ** ** * ** ***** ** ** ** ** ** *****.** **
1409 1397 1448 1442 1400 1394 1532
B. B. B. B. B. B. B.
longum adolescentis angulatum infantis lactis gallicum pseudocatenulatum
TGGCAGATCCAACTTGAGTAAGGAAGAATCCGAGTCTTTGGACGTCAGCTATGTGCAGAA CGGCAGATCCAACCTGAGTGAGGAGGAATCCGAGGCTCTGGACGTTACCTACGTGCAGAA AGGCAGATCCAACCTGAGTAAGGAGGAGTCGGAGTCTTTGGATGTCAGCTACGTGCAAAG CGGTCGCTCCGATTTGTCTGCCGAAGAGAGTGAGGCCCTTGATGTGGCTTACGTGCAGAA CGGCCGCAATGACCTCAATGAGGAGGAGAGCCGTCAGCTTGATGTGAGTTATGTGCAGGA CGGCCGCAATGATCTGGATGAGGCAGAAAGCCGCCAGCTTGATGTGTCGTATGTGCAGGA CGGCCGTTCGGATCTTTCGCAGGAGCAGAGCGAATATTTGGACGTTTCCTATATCGAAAA ** .* :. .* * . *.. *.: . . * ** ** ** .* *...
1469 1457 1508 1502 1460 1454 1592
B. B. B. B. B. B. B.
longum adolescentis angulatum infantis lactis gallicum pseudocatenulatum
CTGGTCCATATTGGGAGGCATTGTGCTGCTTTTCCGCACTGTCGGTGCCGTCGTCACTCA TTGGTCCATGTTAGGTGACGTCGTGCTGCTGTTCCGTACTGTCGGCGCCGTCGTAACCCA CTGGTCTGTGTTGGGAGACATAGTGCTGCTTTTCCGCACAGTCGGTGCAGTCATCACTCA CTGGTCGATTATGGGAGACATTGTGCTTCTCTTCCGCACGGTCGGTGCCGTGCTGAGTCA CTGGAGCGTCATGGGTGATGTCGTGTACATTCTGCGCACGATTGGCACAGTGCTGCACCC CTGGAGCGTGATGGGAGACATCGTATACATTCTTCGTACGGTGGGCACGGTGCTGCGGCC TTGGTCCATTGCAGGCGATCTTGCGATTCTCGCAAAAACCGTGCTCGTGGTCTTCCGCGG ***: .* .** *. * * . : .* .. ** .* . ** * .
1529 1517 1568 1562 1520 1514 1652
R6
B. B. B. B. B. B. B.
longum adolescentis angulatum infantis lactis gallicum pseudocatenulatum
TAAGGGTGCATACTGA CAAGGGTGCATACTGA TAAGGGTGCATACTAA CAAGGGAGCGTACTGA GAGTGGCGCTTACTAG AAGCGGCGCGTATTAA CACCGGATCTTACTAA * ** * ** *..
1545 1533 1584 1578 1536 1530 1668
Figure S2. Nucleotide sequence alignment for the rfbP gene in 7 different Bifidobacterium species. From top to bottom: BIFCAT_01253 (ZP_03324464) from B. catenulatum DSM 16992, BIFPSEUDO_0324 (ZP_03742673) from B. pseudocatenulatum DSM 20438, BIFADO_01843 (ZP_02029386) from B. adolescentis L2-32, HMPREF9003_1455 (ZP_07696327) from B. dentium JCVIHMP022, BL0249 (NP_695455) from B. longum subsp. longum NCC2705, BIFBRE_03332 (ZP_06595517) from B. breve DSM 20213 and Blon_2114 (YP_002323554) from B. longum subsp. infantis ATCC 15697. The grey shadows indicate the primer sets (F3R4 and F5R5) that yielded the best results. B. B. B. B. B. B. B.
catenulatum pseudocatenulatum adolescentis dentium longum breve infantis
ATGCTTCCTTCGCAGAGAGAATACCCCCCTGTTTTCGAGGGTGGGGTGGGGATGGTGGGA 60 -------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------AT 2 ----------------------------------------------------------AT 2 ------------------------------------------------------------
B. B. B. B. B. B. B.
catenulatum pseudocatenulatum adolescentis dentium longum breve infantis
GGAGAAGTCATGCAAG--GTGGTTTCCG--CAGCTCTGAGCAGGATTCG--GATCAAGTA ---------ATGCAAG--GCGGTTTCCG--CAGCTCTGAGCAGGGTTCG--GATCAAGTA ---------ATGCAAGGCGTGAATACCGGTGAATCCGGCGCACCAAACGGCGAGCAAGGT ---------ATGCAGGGTGAGGCAATCG---------------AATTCGACGAGCATG-GGAGGAAGAATTCAGTATCATGCCGAAG--GACAGAGGCACAGCAGCAGAGTCGCAGGCA GGAGGAAGAATTCAGTATCATGCCGAAG--GACAGAGGCACAGCAGCAGAGTCGCAGGCA ------------------------------------------------------------
114 45 51 34 60 60
B. B. B. B. B. B. B.
catenulatum pseudocatenulatum adolescentis dentium longum breve infantis
GTGAGAACCCAGAAAAT--CCATATCGTCTCTTCTGACTCTCGTCATCAGGATCAGAAGC GTGAGAACCCAGGAAAT--CCATATCGTCTCTTCTAACTCTCGTCACCAGGATCAGAAGC ATGAGGACTCAAGAGAT--TCATATCGTCTCGTCCAATGCGCATCGACAGGCATACGCGT -CCCGTACGCAGGAGAT--TCATATGATTTCCTCGCATACGAACCGTCAGACATATGCAA ACAAGCACCGCGACCATGGCAATGCCCAAAGTACGGGCGAGCGGGAGCCGGTCCTCGGCT ACAAGCACCGCGACCATGGCAATGCCCAAAGTACGGGTGAGCGGGAGCCGGGCCTTGGCT ------------------------------------------------------------
172 103 109 91 120 120
B. B. B. B. B. B. B.
catenulatum pseudocatenulatum adolescentis dentium longum breve infantis
AGGAGCGGCATCAG-----TCACAAAACAACAGCCCTCAG--TTTGAGCCGATGACGTTT AGGAGCGACATCAG-----TCTCAAGACAACAGCCCTCAG--TTTGAGCCGATGATGTTT CCATGCAGAATCAG-----CGTCTG--CAAAAG----CAG--TTCGAGCCGATGACTTTT AAACTCAGAATCAGGTAT-ATTCCGGCCAACCTTCCACGTCTTTCGAACCAATGACCTTC TCGTTCTCCCCCGTTGCCGAATGTGACATTGCGCCGACATTCTTTGACGGCACCTACTTC TCGTTCTCCCCCGTTGCCGAATGTGACATTGCGCCGACATTCTTTGACGGCACCTACTTC ------------------------------------------------------------
225 156 156 150 180 180
B. B. B. B. B. B. B.
catenulatum pseudocatenulatum adolescentis dentium longum breve infantis
TCGAACACGCCATTTTC---CGTTGGTGAAGATGGAAGACG-CAAGGGCAGGCG--GAGT TCGAACACGCCATTTTC---CGTTGGTGAAGATGGAAAGCG-CAAGGGCAGGCG--GAAT TCCAATACCCCGTTTCC---TGCGGAAAAGGAAGGACATAGGCGCGGCAAGGCT--GAAT TCCAATACGCCGTTCCCGACCGGAGACAAGGGTGGTATGCGGAGTCGGAGGGCAC-GCAA TCCTCTACACCGGACGGCAGCTCCACACATAACGACACCACGAACATACGTCCATTGATG TCCTCTACACCGGACGGCAGCTCCACACATAACGACACCACGAACATACGTCCATTGATG ------------------------------------------------------------
279 210 211 209 240 240
B. B. B. B. B. B. B.
catenulatum pseudocatenulatum adolescentis dentium longum breve infantis
CAGG---TTGTGCCCGC-ATGGCGTTATTTTTTTGTCGCTTGCCTGGTGATGGTCGATCT CAGG---TTGTGCCCGC-ATGGCGTTATTCTTTTGTCGTCTGCTTGGTGATGGTCGATCT CG-------ACTCCCCG-GTGGCGTTTCGCCTTCGTCGCCAGCCTGATCGCCATCGATTT CGCGGATCGGCGCCCACGATGGCGATATGCTTTCGTAAGTTGCATGGTCGTCCTCGACAT GGCCATTCTGTGCCGAA-GTGGCGATATCTCTATAACACGACCTTGGTCGTGCTTGATCT GGCCATTCTGTGCCGAA-GTGGCGATATCTCTATAACACGACCTTGGTCGTGCTTGATCT ---------GTGCCCAA-ATGGCGTTATCTGTACAATACGACCATGGTGATTCTGGATCT . ** .*****:*: *: .: . : * **.* . * ** *
335 266 263 269 299 299 50
B. B. B. B. B. B. B.
catenulatum pseudocatenulatum adolescentis dentium longum breve infantis
TGCGGTCATGCTGATTTCGCTGTCCATCAGTCTTTGGGTCAATGATGCCGCATATAAT-TGCGGTCATGCTGATTTCGCTATCCATCAGTCTTTGGGCCAACGATGCCGCATATAAT-GGCAGTGATGCTGCTTTCGTTGACCGTCAGCCTTGTAATCAACGGTGCTGCTTTCGAT-CGCGGTGATGCTGCTGTCGCTGCTGATCAGCCTGCTGGTCAACGGCGAAGCATACGTC-GCTGATGACCATTGTGGCCACGTACATCGTGTTCCTGCTCCGACCTTTCGCTTATACCTA GCTGATGACCATTGTGTCCACGTACATCGTGTTCCTGCTCCGACCTTTCGCTTATACCTA GCTGATGACCATCATCGCCACCTGCATCGTATTCGCGTGCAATCCAGGAGCATATGCCAA ..* * .* * * .**. * . *.. **:*: .
393 324 321 327 359 359 110
B. B. B. B. B. B. B.
catenulatum pseudocatenulatum adolescentis dentium longum breve infantis
---GCCATCAATGGGGCTATGCCGTTCTGGCTG-------TTCCTCGTCTGCTTCAGTTT ---GCCATCAATGGGGCCATGCCGTTCTGGCTG-------TTCCTCATCTGCTTCAGCCT ---GCCATCGGCGATGTGATGCCGTTTTGGCTT-------TTCCTCATTCTGTTCAGCCT ---ACCATCGTACGATCCATGCCGTTCGAGCTG-------TTCCTGTTCTGCTTCAGCTT TGTGCAGAGCATTGGTCCCGGCGAATACGGCGTTTTTAGCTTCCTGCTGTTGGCCTGCGT TGTGCAGAGCATTGGCCCCGGCGAATACGGCGTTTTTAGCTTCCTGCTGTTGACCTGCGT TGTGCGGAACATGGGACCATCCGATTATGGTGTATTCAGTTTCCTGAGCTTGGCCTGCAT .* .: . . * .:* .* ***** *:* *
443 374 371 377 419 419 170
B. B. B. B. B. B. B.
catenulatum pseudocatenulatum adolescentis dentium longum breve infantis
CATCTGGGTGTTGAGTCTCACTTTTGCTGGAACGTATCATCGCCATGTGATGGCGGAAGG CATCTGGGTGTTGAGCCTCACCTTTGCCGGAACGTATCATCGCCATGTGATGGCGGAAGG GATTTGGTTGCTGTGCTTGGCATTCGCGGGCACATACCACCGCCATGTTATGGCGGACGG GATTTGGGTGCTGTGCCTGGCACTTGCCGGCACGTACCATCGACATCTCATGGCGGATGG ATCGTGGCTCATCAGTCTGCATTCCGCACGCTCCTATGAACGGCACACCATGGGTGAGGG ATCGTGGCTCATCAGTCTGTATTCCGCACGCTCCTATGAACGGCACACCATGGGTGAGGG CTCCTGGCTCATCAGCCTCTATGCAGCTCGAAGCTACGAACGCCACACTATGGGCGAGGG : *** * * :* * . ** *.: ** * ** ** **** ** **
503 434 431 437 479 479 230
B. B. B. B. B. B. B.
catenulatum pseudocatenulatum adolescentis dentium longum breve infantis
CTACGAGCTGTATGCGAAAATCATCAATGCGTCGCTGCTCACCATCATCACTTACTGCTC CTACGAGCTGTATGCGAAAATCATCAACGCGTCGCTGCTCACCATCATCACTTACTGCTC CTATGAGCTGTATGCCAAAATCATCAATGCGTCGTTGCTGACCATCATCTCATACTGCAG TTATGAACTGTATGCGAAGATTATCAATGCTTCGCTGCTCACCGTCCTGCTGTACTGCAG CTATGCCCTGTACGCCAAGCTGTTGAACGCTGCGTTCATCGATTTCATTATGCTGTGCAC CTATGCCCTGTACGCCAAGCTGTTGAACGCTGCGTTCATCGATTTCATTATGCTGTGCAC ATACGGACTATATGCCAAGCTGCTGAATGCGGCCTTTATCGACTTCATTATGCTCTGTAC ** * **.** ** **..* * ** ** * * .* .. **.* : ** :
563 494 491 497 539 539 290
B. B. B. B. B. B. B.
catenulatum pseudocatenulatum adolescentis dentium longum breve infantis
GCTTGCTTTTATTTTCAATCTTTCCTTGCCGCGAACCGCGCTTATCGTCGCTCCGATCAT GCTTGCTTTTATTTTCAATCTTTCCTTGCCGAGAACCGCGCTTATCGTCGCCCCGATCAT CCTTGCATTTATTTTCAAACTTGCATTGCCCCGCACTGCGTTGATTATCGCGCCAGTCAT CCTGTCGTTCACACTCAATCTCGACCTGCCCCGTACCGCACTGATCACGGCGCCGGTCAT GTTGGGGTACCTGTTCCACCTCAATCTTCCCCGGTCGTTGAATGTGTTCATTCCGCTGGT GTTGGGGTACCTGTTCCACCTCAATCTTCCCCGGTCGTTGAATGTGTTCATTCCGCTGGT ATTGGGCTACTTGTTCCATCTGAATGTGCCGCGTTCCCTGAATGTATTTATTCCTATCCT * *: **.* ** . * ** .* :* . : .* . ** * *
623 554 551 557 599 599 350
B. B. B. B. B. B. B.
catenulatum pseudocatenulatum adolescentis dentium longum breve infantis
TGCATTCTTCCTAGAAGTCATCGCCCGTTGGCAGATGCGTCAGTGGCAGCATCGCAGCCG TGCATTCTTCCTAGAAGTCATCGCCCGTTGGCAGATGCGTCAGTGGCAGCATCGCAGCCG CGCCTTCTTCTTGGACATCATCGCACGCTGGCAGATGCGTCAAGCGTTGCATCGTCACCG CGCGTTCTTTCTGGCGGTGATTGAACGTTGGCAGATGCGTATGAGCCTGCATCGCAACCG TTCACTGGTGTTGGTTATTATCGAGCGTTGGATTATGCGCCGCGCGCTGCACCGCAACCG TTCACTGGTATTGGTTATTATCGAGCGTTGGATTATGCGCCGCGCGCTGCACCGCAACCG TTCTCTCATCCTCGTTATCGTTGAACGGTGGCTGATGCGTCGTGCGCTTCACCGCAATCG * * * * * .* .* *. ** ***.: ***** . : ** ** .. **
683 614 611 617 659 659 410
B. B. B. B. B. B. B.
catenulatum pseudocatenulatum adolescentis dentium longum breve infantis
TCAGAAGGGTGCCTGTAAGTATAAGACCGTGGTGATCGGCTCTTCCGACGGTATCAATCG TCAGAAGGGCGCCTGTAAGTATAAGACCGTGGTGATTGGTTCTTCCGACGGTATCAATTG CAGCAAAGGCTCTTGCAAGTACAGAACGGTGGTCATCGGATCTTCCGATGGCATCAACAA TCGGAACGGGCGGTGCAAATACAAGACCGTGCTGGTCGGATCATCTACCGGCATCAATGC CATGAATGGCGAATTCAACTACCCGACTGTGGTTATTGGGTCTCCGGAAGGTATCCACCG CATGAATGGCGAATTCAACTACCCGACTGTGGTTATTGGGTCTCCGGAAGGTATCCACCG AGCAAAAGGCGAGTTCAACTACCCCACTATTATCATTGGCTCTCCGGAGGGCATCAGTAA ** ** * ** ** . ** .* * .* ** **: * .. ** ***..
743 674 671 677 719 719 470
B. B. B. B. B. B. B.
catenulatum pseudocatenulatum adolescentis dentium longum breve infantis
TGCGTTGCGTACCATGCGGGACTACTCCAATTGGGGGTATTCGCCGATTGCCGTGTGTCC TGCGTTGCGCACCATGCGGGACTACTCCAATTGGGGGTATTCGCCGATTGCCGTGTGTCC AGCCTTGCAGACCATGCGCAACTGCATGAATTGGGGGTGCGAACCAGTTGCTGTATGTCC CGCCCTGCGCACCATGGCCGACTATTCCAATTGGGGCTATGAACCGATCGCAGTCTGCCC GACGGTCGAACAATTGCGCCAGTGCCGTGGCCTCGGCTACGCGCCAATTGCGGTGTGCCC GACGGTCGAACAATTGCGCCAGTGCCGTGGCCTCGGCTACGCGCCAATTGCGGTGTGCCC AACGCTGAAGCAGCTCAGGGCATGCCTCAGCCTGGGGTATGCACCCATCGCGGTCTGCCC .* * . .. * . *. .. ** *. ..** .* ** ** ** **
803 734 731 737 779 779 530
B. B. B. B. B. B. B.
catenulatum pseudocatenulatum adolescentis dentium longum breve infantis
GATCGAAGTTGACGGTAATGAGAAAGACGCTTATGTGGTCACGTCGTTCGAGCCTG---GATCGAAGCTGACGACAATGAGAAAGACGCTTATGTGGTCACGTCGTTCGAGCCTG---AATTGAAGCGGATCCAGCTTCAAAAGACGCTTATGTGGTTACCTCATACACACCGG---GATCGAAGCGGATCTGCACGAGCCCGACGCCTACGTGGTCACCTCATTCGTACCGG---AGTGGTTTCAGTTAAGAACGAGGATGACCCGGATTCGGCGCAGCATTTGGTGTCGGTGCC AGTGGCTTCAGTCAAGAACGAGGATGACCCGGATTCGGCGCAGCATTTGGTGTCGGTGCC TGTTGCCTCTGTGTGTGATTCGGATGACCCGAATGCTGCTCAACATCTTGTATCGGTTCC .* * *: . .. . *** * * * .. . : . . * *
859 790 787 793 839 839 590
B. B. B. B. B. B. B.
catenulatum pseudocatenulatum adolescentis dentium longum breve infantis
-----ATCCTTCCATTGAAGGTGCCGACAAACTGAAGGTGATCCCGTTCAATGCCGCTTT -----ATCCTTCCATTGAAGGTGCCGACAAGCTGAAGGTGATTCCGTTCAATGCCGCTTT -----ATGCCAATATCGAAGGCTCCAGCAAACTCAAGGTGATTCCTTTCAACTCGGCATT -----ATCCCAACATTCCCGGTGCCGACAAACTCAAGGTGATTCCGTTCAATTCGGCATT CTTCACTCCTGCCAACGATGCTGAGGCACGACTGAAGGTGTTGCCACTCAACTCGCATTT CTTCACTCCTGCCAACGATGCTGAGGCACGACTGAAGGTGTTGCCACTCAACTCGCATTT GTTTATTCCTGCCAATGATGAAGAAGCGCGGCTCAAGGTGTTGGCGTTGAACTCCCATTT * * . *: . * . . ...** ******:* * * ** * .:**
914 845 842 848 899 899 650
B. B. B. B. B. B. B.
catenulatum pseudocatenulatum adolescentis dentium longum breve infantis
CCCGCGTACGTGCGAAAGCTTGGGTGCGCAGGAAGTGTACGTTGCCGACGTGCTTTCCCG CCCGCGTACGTGCGAAAGCTTGGGTGCGCAGGAAGTGTATGTTGCCGACGTGCTGTCCCG CCCACGTATTTGCGAAAGCCTTGGTGTACAGGAAGTCTATGTGGCTGACGTGCTTTCGCG CCCTCGTACCTGCGAAAGCCTTGGTGCTCAGGAGGTGTACATAGCCGATGTGTTGTCGCG GCCGCAAACCGCGAAGCGTATGAAGGCACGTACCGTATTGGTAGCTGATGTACTTACCCG GCCGCAAACCGCGAAGCGTATGAAGGCACGTACCGTATTGGTCGCTGATGTACTTACCCG GCCTCAGACCGCCAAGCGGATGAAGGTTCGCACTATTCTGATCGCTGATGTGCTGACGCA ** *. * .*..* * .. * *. .. .* : .* ** ** **. * :* *.
974 905 902 908 959 959 710
F3
B. B. B. B. B. B. B.
catenulatum pseudocatenulatum adolescentis dentium longum breve infantis
TGATTCCGAAATGCTGCATGGCATTTCGCTTGCGGTCGAATCCCTTGGCATGGAGCTTGC CGATTCCGAAATGCTGCATGGCATTTCCCTTGCGGTCGAGTCGCTTGGCATGGAGCTTGC TGATTCCGAAATGCTGCATGGTCTTTCTCTTGCGGTGGAGTCGCTGGGTATGGAACTTGC CGATTCCGAAATGCTGCATGGCATGTCCTTGGCCATCGAATCCCTGGGTATGGAATTGGC TGATTCTGAGACCATGCGTACATTGTCCCTGGCCGTGGAATCCATGGGCATTGAATTGGC TGATTCTGAGACCATGCGTACATTGTCCCTGGCCGTGGAATCCATGGGCATTGAATTGGC TGATTCTGAAACCATGCGTACTCTTTCGCTCGCTGTTGAATCTATGGGCATCGAGCTGGC ***** **.* .***.*. * ** * ** .* **.** .* ** ** **. * **
1034 965 962 968 1019 1019 770
B. B. B. B. B. B. B.
catenulatum pseudocatenulatum adolescentis dentium longum breve infantis
GTTGGCCGTGTCTCTGGCAGATGTGAGCGGTCATCGCCTGTACTTGCGTAATACCGCCGA GTTGGCTGTTTCCTTGGCTGACGTGAGCGGCCACCGCCTGTACTTGCGTAATACGGCCGA CTTGGCGGTCTCCCTGGCGGATGTGAGCGGTCACCGTTTGTATCTGCGAAACACCACGGA GTTGTCCGTATCGTTAGCCGACATCAGCGGCCATCGCCTGTACCTACGTAACACCACGGA CCTCACTACCTCTGTGGCTGATTTGAGCGGCGCTGACCTGCATTTCCGCAACGATCCGAC CCTCACTACCTCTGTGGCTGATTTGAGCGGCGCTGACCTGCATTTCCGCAACGATCCGAC TCTTACTGCATCCGTTGCAGATCTGAGCGGCGCAAATCTGCAGCTACACAATGACCCCTC * * . ** * ** ** * ***** . . ** * * *. ** .. * .
1094 1025 1022 1028 1079 1079 830
B. B. B. B. B. B. B.
catenulatum pseudocatenulatum adolescentis dentium longum breve infantis
ACAGCCTGTATTGTTGGCCAGTTTGCCGCAG--TATACGAATACGACGTACGTGATTAAA ACAGCCGGTATTGCTGGCCAGTTTGCCGCAG--TATACGAATACGACGTACGTGATTAAA ACAGCCGGTTCTGTTGGCTTCCCTGCCGCAG--TACACGGGAGCTGCGAACGTAATCAAG ACAGCCCGTGCTGCTGGCCAGTCTGCCGCAA--TACACCAACTCTGCGTATGCGGTCAAA GATGCCAGTAGTCATTGCACGACTGACGCAG--TACTCAACCGTTACCCGCATCCTCAAG GATGCCAGTAGTCACTGCACGACTGACGCAG--TACTCAACCGTTACCCGCATCCTCAAG TATGCCGATTTTGACAGCCCGACTTGCACAGTATTCTCTGCCG--ACTCGCATCTTTAAG .:*** .* * ** * *.**. *: :* . .* . . * **.
1152 1083 1080 1086 1137 1137 888
B. B. B. B. B. B. B.
catenulatum pseudocatenulatum adolescentis dentium longum breve infantis
CGCCTGCTGGATATCGTTGGTTCGGCTTTCGCGCTGCTGATTTTCTCGCCGCTGATGTTG CGCCTGCTGGATATCGTTGGTTCGGCTTTCGCGCTGCTGATTTCCTCGCCGCTGATGTTG CGTTTGCTGGACATCATCGGCTCACTGTGCGCGCTGATTATTTCCTCTCCGATTCTTCTC CGTGTACTTGATATCGTTTGCTCGGCGCTTGCGTTGATCATATCCTCTCCGCTGATGCTG CGTGTGTGCGATATCGTGCTTTCCGGTATCGCCATTATCTTGAGCTCGCCCATCATGCTA CGTGTGTGCGATATCGTGCTTTCCGGTATCGCCATTATCTTGAGCTCGCCCATCATGCTA CGTGTGTGCGATATCGTTCTCTCCCTGGTGGCAATCATTCTGAGTTCGCCCATCATGCTA ** *. ** ***.* ** ** * .* * : ** ** .* .* *
1212 1143 1140 1146 1197 1197 948
B. B. B. B. B. B. B.
catenulatum pseudocatenulatum adolescentis dentium longum breve infantis
GGTACGGCGCTCGCCATCAAGCTTGATGACGGTGGTCCGATTTTCTTCTCGCAGAAGCGT GGTACGGCGATCGCCATCAAGCTTGATGACGGTGGTTCGATTTTCTTCTCGCAGCAGCGT GGCGTCGCCATCGCCATCAAGCTTGATGATGGCGGTCCGGTGTTCTTCTCTCAGACCCGT GGCGTGGCCATTGCCATCAAATTGGATGATGGTGGTCCGGTGTTCTTCTCACAGACACGT TGGGTGGCATACAAGGTCAAGCGGGAAGACGGCGGCCCTGTGTTCTATTCGCAGACGCGC TGGGTGGCATACAAGGTCAAGCGGGAAGACGGCGGCCCTGTGTTCTATTCGCAGACGCGC TGGGCTGCATATAAGGTTAAGCGAGAAGACGGTGGCCCCGTGTTCTACTCACAGACTCGT * . ** : .. .* **. **:** ** ** * .* ****: ** ***.. **
1272 1203 1200 1206 1257 1257 1008
B. B. B. B. B. B. B.
catenulatum pseudocatenulatum adolescentis dentium longum breve infantis
ATCGGTTTGCATGGCAAGCCGTTTACCATGTATAAATTCCGTTCCATGGTTACCAATGCG ATCGGTTTGCATGGCAAGCCGTTTACCATGTATAAATTCCGTTCCATGGTCACCAATGCG ATCGGACTGCACGGCAAGCCGTTCAAAATGTATAAATTCCGTTCCATGGTCACCAATGCT ATCGGATTGCATGGCCGCCCGTTCAAAATGTATAAGTTCCGCTCCATGGTTACCAATGCC ATCGGCATTTACGGCAAGCCGTTTACCATGTACAAGTTCCGTTCCATGCGCACTGATGCC ATCGGCATCTACGGCAAGCCGTTCACCATGTACAAGTTCCGTTCCATGCGTACTGACGCC ATCGGCATCTACGGCAAGCCTTTCACTATGTACAAATTCCGTTCCATGCGGCTGAACGCC ***** * * ***.. ** ** *. ***** **.***** ****** . .* **
F5
1332 1263 1260 1266 1317 1317 1068
B. B. B. B. B. B. B.
catenulatum pseudocatenulatum adolescentis dentium longum breve infantis
GAAGAATTGAAGAAGAAACTTGCCGAAGAAAATGGACAGACCGATCGTTTCATCTTCAAA GAAGAATTGAAGAAGAAACTTGCCGAAGAAAATGGACAGACCGATCGTTTCATCTTCAAA GAGGAGCTGAAGAAGAAACTCGCTGAGGAAACTGGGCAGGAAGACCGGTTCATCTTCAAA GAGGAGTTGAAGAAGAAACTCGCAGAGGAAAATGGCCAGTCCGATCGTTTTATTTTCAAG GACGAGATCAAGGCGAAACTGGCTAAGGAACGCGGCATTGAGGATCGTTTTATCTTCAAG GACGAAATCAAGGCGAAATTGGCTAAGGAACGCGGTATTGAGGATCGTTTTATCTTCAAG GATAAGATGGATGCCGAAGTGGCTGCCGCAGCTGGCGTGGAACTGGGAACAACATTCAAG ** .*. * .* .. .** * ** .. *.* ** : . : * : * *****.
1392 1323 1320 1326 1377 1377 1128
B. B. B. B. B. B. B.
catenulatum pseudocatenulatum adolescentis dentium longum breve infantis
ATGAAGGATGATCCGAGAATTACGAAGGTTGGTCGTTTTATTCGTAAGACTTCGTTGGAT ATGAAGGATGATCCGAGAATTACGAAGGTCGGTCGTTTTATTCGTAAGACTTCGTTGGAT ATGAAAGATGATCCGCGTATCACCAAGGTAGGTCACTTCATCCGTAAGACTTCTTTGGAT ATAAAAGACGATCCGCGCATTACCAAAATCGGTCATTTTATTCGAAAGACCTCACTGGAT CTCAAGGACGATCCACGCGTGACTAAGATCGGTCACTTTATTCGTAAAACATCGCTGGAT CTTAAGGACGATCCACGCGTGACCAAGATTGGTCATTTCATTCGTAAGACGTCGCTGGAC GTCAAGGACGATCCTCGTGTTACTAAAATTGGTAAGTTTATCCGTAAGACTTCCATTGAC * **.** ***** .* .* ** **..* ***.. ** ** **:**.** ** * **
1452 1383 1380 1386 1437 1437 1188
B. B. B. B. B. B. B.
catenulatum pseudocatenulatum adolescentis dentium longum breve infantis
GAATTCCCACAGTTCTTCAACGTTTTGAAGGGCGACATGTCTTTGGTTGGTCCTCGCCCG GAATTCCCCCAGTTCTTCAACGTTTTGAAGGGCGACATGTCTTTGGTTGGTCCTCGCCCG GAGTTCCCGCAGTTCTACAATGTGCTCAAGGGCGATATGTCTCTGGTCGGTCCGCGCCCG GAATTCCCGCAATTCTTCAACGTCTTGAAAGGTGACATGTCGTTGGTAGGGCCGCGCCCA GAGTTCCCGCAGTTCTTTAACGTGTTCAAGGGCGATATGAGCTTGGTTGGGCCTCGCCCG GAATTCCCGCAGTTCTTTAACATGTTCAAGGGCGATATGAGCTTGGTTGGTCCTCGCCCG GAGGTTCCTCAGTTCTTCAATGTGCTGAAGGGCGATATGTCCATGGTGGGTCCGCGTCCG **. * ** **.****: ** .* * **.** ** ***: **** ** ** ** **.
1512 1443 1440 1446 1497 1497 1248
B. B. B. B. B. B. B.
catenulatum pseudocatenulatum adolescentis dentium longum breve infantis
GCTTTGCCGGAAGAGGTTGCCCGCTATGGTTCCCTTTATTCCGCGCGACTGCTGGTTAAG GCTTTGCCGGAAGAGGTTGCTCGCTATGGTTCCCTTTATTCCGCGCGACTGCTGGTTAAG GCATTGCCGGAAGAAGTGGCCCGCTATGGTTCCCTATATTCCGCCCGACTGCTGGTCAAG GCACTGCCTGAGGAAGTGGCACGGTACGGGTCATTATATTCGGCGCGACTGTTGGTCAAG CCGCTGCCTGAAGAAGTCGCGCGATATGACATGCTGTATTCCACGAGACTGCTGGTTAAG CCGCTGCCTGAAGAAGTCGCGCGATATGACATGCTGTACTCCACACGACTGCTGGTTAAG CAACGTCAATATGAGGTTGACCAGTACTCGCCATTGTATTCCACTCGCCTACTGGTCAAG . *. * **.** *. *. ** * ** ** .* .*.**. **** ***
1572 1503 1500 1506 1557 1557 1308
B. B. B. B. B. B. B.
catenulatum pseudocatenulatum adolescentis dentium longum breve infantis
CCTGGTATTGCCGGTCCTTGGCAGGTTTCCGGCCGTTCGGATCTTTCGCAGGAGCAGAGC CCTGGTATTACCGGTCCGTGGCAGGTTTCCGGCCGTTCGGATCTTTCGCAGGAGCAGAGC CCGGGCATCACCGGTCCATGGCAGGTGTCCGGCCGGTCCGATCTTTCACAGGAGCAAAGC CCCGGCATCACTGGACCGTGGCAGGTTTCCGGACGCTCCGATCTTTCCCAAGAGCAAAGT CCTGGTATTACTGGACCGTGGCAGATTTCCGGACGCAGCGATTTGACTCAGGAACAGTCG CCTGGTATTACTGGACCGTGGCAGATTTCCGGACGCAGTGATTTGACTCAGGAGCAGTCG CCGGGTATCACCGGTCCTTGGCAGATTTCCGGGCGTAATGATTTGTCTCAGGAACAGTCT ** ** ** .* **:** ******.* ***** ** : *** * :* **.**.**.:
B. B. B. B. B. B. B.
catenulatum pseudocatenulatum adolescentis dentium longum breve infantis
GAATATCTGGACGTTTCCTATATCGAAAATTGGTCCATTGCAGGCGATCTTGCGATTCTC GAATATTTGGACGTTTCCTATATCGAAAATTGGTCCATTGCAGGCGATCTTGCGATTCTC GAATATTTGGACGTCTCCTACATTGAGAATTGGTCCATCGCTGGAGATCTTGCGATTCTT GAATATTTGGACGTTTCCTATATCGAAAATTGGTCCATTACAGGCGATTTAGCCATTCTT GAGTATGCGGATGTGTCGTATATTCAGGACTGGTCTATCACGGGGGACATCGTGATTCTG GAGTATGCGGATGTGTCGTATATTCAGGACTGGTCTATTACGGGGGACATCGTGATTCTG GAATATGCAGATGTGTCATATATTCAGAATTGGTCTATCACGGGGGATATCGCGATTCTG **.*** .** ** ** ** ** *..* ***** ** .* ** ** * * *****
R4
R5
1632 1563 1560 1566 1617 1617 1368
1692 1623 1620 1626 1677 1677 1428
B. B. B. B. B. B. B.
catenulatum pseudocatenulatum adolescentis B. dentium longum breve infantis
GCAAAAACCGTGCTCGTGGTCTTCCGCGGCACCGGATCTTACTAA GCAAAAACCGTGCTCGTGGTCTTCCGCGGCACCGGATCTTACTAA GCCAAAACCGTGATGGTAATCTTCACTGGCCGTGGATCGTACTGA GCGAAAACAGTTCTAGTGGTATTCCGCGGAACGGGATCATATTAA TTGAAGACTGTGGTGGCAGTGTTTAAGGGGACTGGATCGTACTGA TTGAAGACTGTGGTGGCAGTGTTTAAGGGGACTGGATCGTACTGA CTGAAGACAGTGGGCGCGGTATTTAATGGAACTGGTATGTAG--**.** ** * ..* ** . ** . **:: **
1737 1668 1665 1671 1722 1722 1470
Degenerate PCR primers for detecting putative priming glycosyltransferase genes in Bifidobacterium strains C. Hidalgo-Cantabrana, I. Ordoñez, P. Ruas-Madiedo and A. Margolles Figure S1. Nucleotide sequence alignment for the cpsD gene of 7 different Bifidobacterium species. From top to bottom: BL0237 (accession no. NP_695447) from B. longum subsp. longum NCC2705, BAD_1389 (YP_910252) from B. adolescentis ATCC 15703, BIFANG_03177 (ZP_04448174) from B. angulatum DSM 20098, BLIG_00247 (ZP_04665465) from B. longum subsp. infantis CCUG 52486, BLA_0595 (YP_002469468) from B. animalis subsp. lactis AD011, BIFGAL_04272 (ZP_05966491) from B. gallicum DSM 20093 and BIFPSEUDO_03247 (ZP_03742673) from B. pseudocatenulatum DSM 20438. The grey shadows indicate the primer sets (F1R2 and F4R6) that yielded the best results. B. B. B. B. B. B. B.
longum adolescentis angulatum infantis lactis gallicum pseudocatenulatum
--------------------------TTGGAGAGAAGGATGT-CCCAAAGCGGTAGCGCT --------------------------------------ATGG-CTTACAGTGGCAATAGT TTGAAGGTTGCCCCCTCCTATGCGCTTTGGGGGGAGAGCCGG-CAGGAGAAAGAAGACAT --CTGAGCAGTAACGGTCAGCCGTCTACACGCAGCAGACAGG-CCAATAGCGGTGACCGA --------------------------------ATGGCACAGG-CGAAAAG-------CGA --------------------------------ATGTCACAGG-CCAAGACGG-----CTA ----ATGCAAGGCGGTTTCCGCAGCTCTGAGCAGGGTTCGGATCAAGTAGTGAGAACCCA . * * . . :
33 21 59 57 20 22 56
B. B. B. B. B. B. B.
longum adolescentis angulatum infantis lactis gallicum pseudocatenulatum
GAAACGA-------------ATCTGAGTGAAACCTCCCGTAAACGGGTGCTTAGCGTCGC GGGGAGA-------------AACTGAGCGAGACCTCACGCAGGCATGTGCTCAGCGTTGC GACGCAAGCCAATAGCACCGCACTGAAAGACACATCGTGGCAGCAGCTGGTGAATGTTGC ACCACCG----------CTCAGCTCAGTGATATCCGCCAAAAGCATCTCAGCCACGTGCT TGCTCAG-------------CTCGCTGAGGAAGTGGACGTCCGCTCCAGCAGGGTGCTGC AACTCGC-------------TGACCTTACGCAACAGAGGTCAGCGCATACAT-----TGC GGAAATCCAT---------ATCGTCTCTTCTAACTCTCGTCACCAGGATCAGAAGCAGGA . : * . . * :
80 68 119 107 67 64 107
B. B. B. B. B. B. B.
longum adolescentis angulatum infantis lactis gallicum pseudocatenulatum
TTATTGGCGTTTTTTCGTCAATGCTCTTTTGATT-----------CTGTGCGACATGGCT CTACTGGCGTTTCTTCGTCAACGCCCTGTTGGTG-----------TTGTGCGATATGGCT GTATTGGAGGTTCTTCGTCAATGCGGCATTGACT-----------GTTTGCGATATGGCC GTACTGGCGATTCTTCGTCAATCTCGCCCTCATG-----------CTTATCGACGCCGCC G--ATGGCGTTTCGTCATGAACCTGGCACTCATG-----------CTGTTCGATGCGTTG G--GTGGCGATTCTCGTTGATTCTGCTGCTGATG-----------GTGCTTGATTTTCTT GCGACATCAGTCTCAAGACAACAGCCCTCAGTTTGAGCCGATGATGTTTTCGAACACGCC . .. * : *: : * **
129 117 168 156 114 111 167
B. B. B. B. B. B. B.
longum adolescentis angulatum infantis lactis gallicum pseudocatenulatum
TCATTT--------------ATTGGCGCTTCGGCAATCATATATGTGTGG---CAGGGCG GCCTTC--------------ATCATCGCTTCGGCGATCGTATACGTGGTG---CGGAATA GCGTTT--------------GTGCTCGCTTCGGTGATTGTGCTTATGGCG---CGCAATG ATGTAC--------------ATCATCGCCGGAGCCACGGTGCTCAATCTG---CGTCATG GTCTAC--------------TTCGCTACCAGCATGATTGTGCTGGTCGTA---TCGCATG GTGTAT--------------GCCG-TGGTGTCAT--TCATTCTTATCGAG---GACTTTG ATTTTCCGTTGGTGAAGATGGAAAGCGCAAGGGCAGGCGGAATCAGGTTGTGCCCGCATG *: . . . : . . .
172 160 211 199 157 151 227
B. B. B. B. B. B. B.
longum adolescentis angulatum infantis lactis gallicum pseudocatenulatum
GGGAAGC---GTTGTATTCTTCCCG----------------------------TTTC--CCGGTGA---TTTATATTCCACGCG----------------------------TTTC--AGGCGGC---GCTGTATTCCGCTCG----------------------------TTTC--AGGCAACGCCGTTCTATTCGAGCAG----------------------------ATTC--GCACGAA---TCTAGTGACGACTCG----------------------------TTTG--ACGAGCT---CAGGCAGCCGGTGAT----------------------------TTTC--GCGTTATTCTTTTGTCGTCTGCTTGGTGATGGTCGATCTTGCGGTCATGCTGATTTCGCT . * :**
198 186 237 228 183 177 287
B. B. B. B. B. B. B.
longum adolescentis angulatum infantis lactis gallicum pseudocatenulatum
-----------------------------CATTT------CCATATTGGTTTGTGGC-------------------------------CACTT------CCATATTGCTTTGTGGG-------------------------------CATTT------CCATATCAATCTGGCCG-------------------------------AATTT------CACGATTGACTCCACCG-------------------------------CAACTGAATCTGCCGATAGACCCGTGGC-------------------------------CGTTTTGATGTGCCGATGAACGCGTTCT--ATCCATCAGTCTTTGGGCCAACGATGCCGCATATAATGCCATCAATGGGGCCATGCCGTT .. * . ** .
220 208 259 250 211 205 347
B. B. B. B. B. B. B.
longum adolescentis angulatum infantis lactis gallicum pseudocatenulatum
-----TGTATGCGCTTGCTTGCTCGTTGATTTGGATCTACTGCCTGCATAGTGTTGGCGT -----CGTATATGCTTGTTGGGTCGCTGATTTGGATATATTGCCTGCACAGCGTAGGCGT -----TGTATGTGATTGTGTGCGCGCTGATTTGGGCGCTGTGCATGCACAGCGTGGGCGT -----TTTACTTTATGATTGTGGCCTGCTTCTGGGTGTGGTCGCTCTATGTCGCCGGCAT -----TGTATTCCGCTGCCTGCGCGGCGATTTACGTCTACTGCATGCATGTGGCGGGGGT -----GGTACATCGTTATTTGCTCGCTGATTTATGTGTGCTGCCTCAAGATGTCTGGCGT CTGGCTGTTCCTCATCTGCTTCAGCCTCATCTGGGTGTTGAGCCTCACCTTTGCCGGAAC *: :* *. . : .* . ** .
275 263 314 305 266 260 407
B. B. B. B. B. B. B.
longum adolescentis angulatum infantis lactis gallicum pseudocatenulatum
GTACCACCGTCATGTGATGAGTGACGGTTACCAGTTGAACGCGCTACTGGTCAAAGGCAC GTACCACCGTCATGTGATGGGTGATGGTTACCAGCTCAATGCGTTCCTGATCAAGGGCGC GTATCACCGGCATGTGATGGGTGACGGCTACCAGCTGAACCTGCTGCTGTTCAAGGGCGC TTATCATCGCCATGTTATGGGTGACGGTTACCAGCTCAATGTGCTGCTGGTCAAAGGCAT GTACCACCGGCATGTGTTCGCCGACGGATACCAGCTCAACATGTTGCTACTCAAGGGTGG GTATCATCGCCATGTGCTTGGTGACGGTTATGAGCTCAACGTGCGTTTGCTCAAAG-CGG GTATCATCGCCATGTGATGGCGGAAGGCTACGAGCTGTATGCGAAAATCATCAACGCGTC ** ** ** ***** * . ** ** ** ** * :* * * **** *
335 323 374 365 326 319 467
B. B. B. B. B. B. B.
longum adolescentis angulatum infantis lactis gallicum pseudocatenulatum
GTTTTTCACGGGATTGATGATT-TGTGCAGTTGATTCTATTCTCAACGTTTATATACCTT GCTGTTCGCGGGTTTGATGGTT-TGCGCGTTGAATTCCATTCTCAACGTGTATGTGCCTC GGTGCTCGCCGGCATTATGGTG-TGCGCGGTCGATTATATTCTCAACTCGTATATTTCCC GGTCGTGTGCTGGGTGGTGCAG-TGCGCGTTCAGCTACTTCTTCAATCTGGGTCTGACCC CTTCATTGCCTGGGTGGCGATT-TGCGCGCTGAATTTCATCATTCAGTGCAACATATTCT CCGTAATGGATTTCTGCCTCGTGTGCTCGCTCAATTATGTGCTCGAATCTAAAATCGGCA GCTGCTCACCATCATCACTTAC-TGCTCGCTTGCTTTTATTTTCAATCTTTCCTTGCCGA : * ** *. * . * * * * *
394 382 433 424 385 379 526
B. B. B. B. B. B. B.
longum adolescentis angulatum infantis lactis gallicum pseudocatenulatum
TGCTGCATACCGGCTTGGTGTTGCTGTGCGCATTCCTGATTACCATGCTGGAGCGTTTGA TGGTGACCACTGGATTGGCGCTGCTGTGCGCGTTGCTGGTCACCATTGTCGAACGTTTCA TGCTTTCCACGGCGCTGATCATGGTGTGCGCGCTGCTGATCACCATGGCTGAGCGGTTTG TTACCTCGCTCTGCTTGGTGGTGATTGCCGGATGGTTGCTCACCATGGTGGAACGCATTG TCTACATGGTCTGCCTCACCTCGTTGATTGTGTGGGTGGCGGAAATGATCGTTCGCTTCA TCCGCATGGTCGTGCTGACGTCGCTGTTCGCGTGGATCGCCACGATGATTATGCGTATGA GAACCGCGCTTATCGTCGCCCCGATCATTGCATTCTTCCTAGAAGTCATCGCCCGTTGGC * . * * * . * .. .* . ** :
454 442 493 484 445 439 586
F1
B. B. B. B. B. B. B.
longum adolescentis angulatum infantis lactis gallicum pseudocatenulatum
TTATCCGCCAGTTTGTGCTGTCCAGCCGTGAAAAGGGCGCATATTCGTATGGCACCGTGG TCATTCGTCAGTGCGTGCTGTTCAGCCGTGAGAAGGGCGCGTATTCGTATGGCACCGTGG TGATTCGCCGCCTGGTGCTGTTCGGCCGTGAGAAGGGCGCCTACTCGTATGCCACGGTGG CCAGCCGTGCATTCATTACGCGTAGTCGTCGCAAGGGCGCCTACGCTTACGGCACGGTGA TCGCGCGCGTCATTCTCACCAGTAAACATCGCAAAGGGGAGTATTCGTACCCGACCGTCA TTGTGCGCTTTATTGTGACGCGCCGCCGGCGTAAAGGTGCCTATACCTACCCGACAGTAA AGATGCGTCAGTGGCAGCATCGCAGCCGTCAGAAGGGCGCCTGTAAGTATAAGACCGTGG . ** : . . *. . **.** *. *. . ** ** ** .
514 502 553 544 505 499 646
B. B. B. B. B. B. B.
longum adolescentis angulatum infantis lactis gallicum pseudocatenulatum
TGGTTGGTTCCCCTGATGGTATTGAACATGCTTTGGAATTTCTGAGCCAGAAGCGTCAAC TGGTCGGCTCGCTGGAGGGCATTGAGCATGCCTTGCAGTTTTTGAGCCGGAAGAGCCAGC TTGTTGGCTCGTGCGAGGGCATTGAGCATGCGTTGCGTTTTCTGAGCCAGAAGCAGCAGT TCATCGGTTCCCCGCACGGCATTGGTCGCACGCTAAAATTCCTCGGTCAGCGTCGGCAAC TCGTCGGTTCCCCCGAGGGTATTGGGCAGATTCTCAAGTTCCTCAATCAACGCAGACAGC TTGTCGGGTCTCCGGAGGGTATTGGGCAGGCGCTCAAATTCATTAATAATCGCAAGCAAC TGATTGGTTCTTCCGACGGTATCAATTGTGCGTTGCGCACCATGCGGGACTACTCCAATT * .* ** ** * ** ** .. . . * . : * . . . .*
574 562 613 604 565 559 706
B. B. B. B. B. B. B.
longum adolescentis angulatum infantis lactis gallicum pseudocatenulatum
TGAATTATCATGCTATTGCGGTGTGCCCGATTGGTTTGAACAAGCGGACTGGGCTCGTGG TGAACTACCATGCCATTGCGGTGTGCCCGATTGGGCTGGATGCGCAGACCGGCTATGTAA TGAATTACCGCACGGTGGCGGTGTGCCCGATTGGCTTGAATGCGCAGACCGGGCATATTG TCAACTACCGCCCGGTGGCAGTATGCCCGATTCATCTCAATCCCGATACCGGATTGATTG TCAATTACAAGCCAATTCGCCTGTGCCCGATTCGGGTCAATAGCGAGACGGGGCTCATTG TCAATTACAAGGCCATTGCCGTGTGCCCGATCCGCGTGAACCGCAAGACGGGTTTGATTG GGGGGTATTCGCCGATTGCCGTGTGTCCGATCGAAGCTGACGACAATGAGAAAGACGCTT .. ** * .* *.** ***** . .* . .. .. : .
634 622 673 664 625 619 766
B. B. B. B. B. B. B.
longum adolescentis angulatum infantis lactis gallicum pseudocatenulatum
AGGCCGTTGTCGCACCTGCTGAT-TTCAAGGATCGAATCCGCAAGATCAG-TGGTAGGGA AGGCCGTTGATGTTACCGCTGAA-TTTAAGCAGCGGATCCGTGAGATTTG-TGGTAGGGA AGGCTGCCAGCGCACCCGAGGAG-TTTATGCAGGAGATTCGCCGGATTGG-TGGTAAGGA AACAGAGCACCGACCATGAGACA-CTCCGTGAGGAGATGCAAAAGAACGA-GGGCTGCCA AGGCGGATCCGAACACCGAATTG-CTCAGCAAGGAAGTGCATGAGTCATGGAATTCCCCG AGGCTGATGACGATTACGAGGCG-CTCAAGACACAGATGAAAG-GCCATGGTGGCCACGG ATGTGGTCACGTCGTTCGAGCCTGATCCTTCCATTGAAGGTGCCGACAAG-CTGAAGGTG * . *. * . . ..: * . .
692 680 731 722 684 677 825
B. B. B. B. B. B. B.
longum adolescentis angulatum infantis lactis gallicum pseudocatenulatum
CATCGTCACATTGCCGTATTGCGG---GAACTTCGCCGAACGTGCCGCGTCCATGGGCGT TATCGCCACGTTGCCATATTGCAA---GAATTTTGCCGAACGCGCCGCGTCCATGGGCGT GATTCCCGTGCTGGCGTATGGCGG---CGATCTGGCCGAACGTGCTGTGGCCATGGGCGC GCTGCCCGTACTCGAGTACAGCGATCATAATCTTGCTGAACAGATTATTGACATGAACGC ATTGATGTGATTGA-ATATGGACC---CCAGCTGGCTGGACGGATTGCGGAAACCGGCGC CATTATGCCGGTGCGCTATGGCGA---GAACATGGGGGAGCGGTTCGCCCAGCTTGGCGC ATTCCGTTCAATGCCGCTTTCCCG------------CGTACGTG-CGAAAGCTTGGGTGC * . * : . * .*. . .. *
749 737 788 782 740 734 872
B. B. B. B. B. B. B.
longum adolescentis angulatum infantis lactis gallicum pseudocatenulatum
GCAGACGGTGATGGTTACGGATGTTATGCAGCGCTTCTCGGATAATTTCAACACGTTTGT GCAGACGGTGATGGTTACTGATGTGATGCACCGGTTCTCGGACAATTTCAACACGTTTGT ACAAACCGTGATGGTGACGGATGTGCTGCAGCGTTTCTCCGACAATTTCAATACGTTTGC GCAGACCGTTATGGTGGCTGACGAGCTGCATCGCTATTCGGATAATTACGATATCTTCTC ACAGACGGTGATGGTCACCGATGTGCTCGACCGATTCTCCGACAATCTGAGCACTTTTGC GCAGACGATCATGGTTACCGATGTGCTCGACCGGTTCTCCGACAACCTCAACATGTTCTC GCAGGAAGTGTATGTTGCCGACGTGCTGTCCCGCGATTCCGAAATGCTGCATGGCATTTC .**... .* :: ** .* ** *: .* . ** : ** ** *: : . . :*
809 797 848 842 800 794 932
B. B. B. B. B. B. B.
longum adolescentis angulatum infantis lactis gallicum pseudocatenulatum
GCTGAACATGGAAACCATGAATCTGGAGATTGCGGTGGTCACTTCTGCAGTGGACGTGAG GCTGGACGTGGAGGCCATGAACTTGGAAGTGGCGTTGATTACCTCCGCGGTGGATGTGAG GTTGAGTCTTGAATCCATGAACCTGGAGATTGCGCTGGTTACCTCTGCCGCTGATGCGAG CGTGCGCATGGAATCGTTCGGTCTGGAGATTGCCGTGCTGGCTTCCGCCGCTGACACTTC TTTGCAGATGGAATCCGTGAATCTTGAGATTGCGCTGATCACCTCTGCGCTTGACATTGC GCTGCAAATGGAAGCCATGAACCTTGAGGTCGCGCTCGTCACGTCCGCAGTGGACATCGC CCTTGCGGTCGAGTCGCTTGGCATGGAGCTTGCGTTGGCTGTTTCCTTGGCTGACGTGAG * * **. * * .. * **. * ** * . ** ** .
869 857 908 902 860 854 992
B. B. B. B. B. B. B.
longum adolescentis angulatum infantis lactis gallicum pseudocatenulatum
TGGTCATGAGACCAACATCCGTGTGATTCAGGGTACGACGGTGATGACGATCAGCCTACC CGGCCATGAGACCAACATCCGTGTGATTCAGGGTTCCACGGTGATGACCATCAGCCTGCC CGGCCATGAGACGAATGTGCGTGTGATTCATGGCGCTACGGTGATGACCATCTGTCTGTC CAACCATGAGATTCAGGTTCGATCCATACAGGGCACCACGATTATCACGCAACGTCTTGC CGAGCATGCCACGCAGATGCGCAACATTCAGGGTATGAGCATCATGACGATCAGTCTGCC GGGCCATGCCACGCGCATCCAAAACCGTAACGGCCTGACGATCATGACGATTTACTTGCC CGGCCACCGCCTGTACTTGCGTAATACGGCCGAACAGCCGGTATTGCTGGCCAGTTTGCC .. ** . . * *. . . *. . .* :* . . * *
929 917 968 962 920 914 1052
B. B. B. B. B. B. B.
longum adolescentis angulatum infantis lactis gallicum pseudocatenulatum
GCAGTATTCGCCATGGGATATGTGCAAAAAACGTGTTTTTGATGTGCTGGTTTCGCTGTT GCAGTATTCGCCGTGGGCCATGTTCAAGAAGCGTGTGTTCGATATTGTGGTGTCGCTGAT GCAGTATTCGCCGGCTGTGATGCTGCAGAAGCGTGTGTTCGACGTTGTGGTCTCGCTGCT GCAGTACACGCCAGCCCGCCGTATGGCTAAGCGCCTGTTTGACTTGGTGATTTCTTCGCT TCAGTATTCCCCCTCCGCACAGATTAAGAAACGCGTGTTCGATCTCGTGGTGTCGACGAT GCAGTATTCGCCATATGAGCAAATCAAGAAGCGCATCTTCGACATTGTGGTTTCGAGTGT GCAGTATACGAATACGACGTACGTGATTAAACGCCTGCTGGATATCGTTGGTTCGGCTTT ***** :* .. **.** * * ** * * . ** *
989 977 1028 1022 980 974 1112
B. B. B. B. B. B. B.
longum adolescentis angulatum infantis lactis gallicum pseudocatenulatum
TGCCGTGATTGTTGCTGCGATTGTTACCGTTCCTATTGCGATTGCTATCAAGCTTACCGA GGCATTGGTTGGCACCGCGATTATTACCATCCCGGTGGCCATTGCCATCAAGGTGACCGA CGCATTGGTGGGTACGGCCATCATCACTGTTCCGGTGGCCATTGCCATCAAAGTGACCGA AGCGATCATCTGTTCGTTGATTATCACCATCCCGGTGGCGATCGCCATCAAAGTCACGGA TGCGATCATTCTGAGCTCGCCGATCATGCTGATCGTGGCGTTGGCGATCAAGATTGAAGA GGCCCTTGTGGTGTCGTCGCCAATCATGGCCATCGTGGCTTTGGCCATCAAGATGGAAGA CGCGCTGCTGATTTCCTCGCCGCTGATGTTGGGTACGGCGATCGCCATCAAGCTTGATGA ** * * . * * . ** :* ** *****. * .. **
1049 1037 1088 1082 1040 1034 1172
B. B. B. B. B. B. B.
longum adolescentis angulatum infantis lactis gallicum pseudocatenulatum
CGGCGGTCCTATTTTCTACACGCAGACTCGAGTTGGGCGCCGTGACAAGCCGTTTAAGAT TAGAGGGCCTATTTTCTACACTCAGACCCGTGTGGGTCGCCGTGGCAAGCCGTTCAAGAT TGGCGGGCCGATCTTCTATACGCAGACCCGTGTAGGCCGCCGTGGCAAGCCGTTCAAGAT CGGCGGACCGGTGTTCTACAAGCAGACCCGTATGGGCCTGCGCGGCAAGTCGTTCCAGAT CGGCGGTCCGGTGTTCTATAAGCAGGAGCGTATCGGCTTGCGCGGCAAGCCGTTCTATAT TGGCGGCCCAGTGTTCTATAAGCAGGAGCGCATCGGATTGCGCGGCAAGCCGTTTTATAT CGGTGGTTCGATTTTCTTCTCGCAGCAGCGTATCGGTTTGCATGGCAAGCCGTTTACCAT .* ** * .* ****: :. *** . ** .* ** *. *.**** **** . **
1109 1097 1148 1142 1100 1094 1232
B. B. B. B. B. B. B.
longum adolescentis angulatum infantis lactis gallicum pseudocatenulatum
GATCAAGTTCCGTTCTATGGTGGTGGATGCGGACAAGATGAAGGCAGAACTGGCGAAAGA GATTAAGTTCCGTTCCATGGTGGTGAACGCCGACAAGATGAAGTCGGAACTGGCGGAGCA GATTAAGTTCCGTTCCATGGTGGTGAATGCCGACAAGATGAAGGCTGAGCTGGCTGCGGA GTACAAGTTCCGTTCGATGGTGGTGAACGCCGACGCGCTCAAGAAGGAGCTCGCAAAAGA CCATAAGTTCCGTTCGATGCGTGTGGATGCCGACGCACACCTGAAAGAGGTATTGGCTGC CCACAAGTTCAGGTCGATGTATGTCAATGCGGACGCGCGCCTTGAGGAAGTGCTCAAAGA GTATAAATTCCGTTCCATGGTCACCAATGCGGAAGAATTGAAGAAGAAACTTGCCGAAGA : **.***.* ** *** . .* ** **.... .: . .*. * .. .
1169 1157 1208 1202 1160 1154 1292
F4
R2
B. B. B. B. B. B. B.
longum adolescentis angulatum infantis lactis gallicum pseudocatenulatum
AACCGGTCAGGATGGTCGTTTTATCTTCAAGATGAAGGACGATCCGCGAGTCACCAAGGT GACCGGGCAAAAGGGACGGTTCATCTTCAAGATGAAGAACGATCCCAGAGTCACTACAGT GACTGGGCAGAAGGGTCGTTTCATTTTCAAGATGAAGGACGATCCGCGAGTCACCAAGGT AACCGGCCAGACCGACCGCTTCATCTTCAAGATGAAGAACGACCCACGCATCACCAAGGT GCAAGGGCAGGAATTGGGAGCCCGGTACAAGCTCAAGCACGACCCGCGCATCACGAAGGT ACAAGGTCAGGAACTTGGCGCCCGATACAAGCTCAAGCATGATCCGCGTATCACCAAGGT AAATGGACAGACCGATCGTTTCATCTTCAAAATGAAGGATGATCCGAGAATTACGAAGGT ... ** **... * . *:***..* *** * ** ** .* .* ** *..**
1229 1217 1268 1262 1220 1214 1352
B. B. B. B. B. B. B.
longum adolescentis angulatum infantis lactis gallicum pseudocatenulatum
GGGCAAGTTTATCCGTAAGTTCTCCATTGACGAGTTTCCGCAGTTCCTGAACGTGTTGAA GGGCCGGTTCATCCGCAAGTTCTCCATCGACGAGTTCCCGCAGTTCCTGAACGTGTTGAG GGGCAAGTTTATCCGCAAGTTCTCCATCGATGAATTCCCCCAGTTCCTCAATGTGCTCAA GGGGCATTTCATTCGCCGCTTCTCCATTGACGAGTTGCCGCAGTTCCTCAACGTGTGGAT GGGTAACTTCATTCGCAAGACGAGTCTCGACGAGCTTCCGCAGTTCTTCGATGTGTTTAT CGGCGCATTCATCCGCAAGACGAGCCTGGACGAGTTGCCGCAGTTCTTTGACTCGTTCCG CGGTCGTTTTATTCGTAAGACTTCGTTGGATGAATTCCCCCAGTTCTTCAACGTTTTGAA ** ** ** ** .. : : * ** **. * ** ****** * .* .
1289 1277 1328 1322 1280 1274 1412
B. B. B. B. B. B. B.
longum adolescentis angulatum infantis lactis gallicum pseudocatenulatum
GGGCGATATGAGCGTGGTGGGCCCGCGTCCGCCGTTACCTGAGGAGGTGGCTCAATACAA GGGCGATATGAGCGTGGTGGGCCCGCGCCCGCCGCTGCCTGAGGAAGTGGCCCAGTACAA GGGAGATATGAGCGTGGTGGGCCCGCGCCCGCCGCTGCCCGAGGAAGTGGCTCAATACAA GGGGGATATGTCCGTAGTGGGGCCGCGCCCGCCGCTGCCTGAGGAATATGCCCGTTACAA GGGTTCGATGAGTGTGGTGGGGCCGCGCCCGCAACGTTCATTCGAGGTGGCCGAGTATGA GGGCGCCATGAGTGTGGTGGGTCCACGACCACAGCGCGATTTTGAAGTGCGTGAATATGA GGGCGACATGTCTTTGGTTGGTCCTCGCCCGGCTTTGCCGGAAGAGGTTGCTCGCTATGG *** . ***: *.** ** ** ** **. . . : **. : . ** ..
1349 1337 1388 1382 1340 1334 1472
B. B. B. B. B. B. B.
longum adolescentis angulatum infantis lactis gallicum pseudocatenulatum
CCAGACGTATGCCACTCGTATGCTGGTGAAGCCTGGTATTACCGGCCCGTGGCAGGTGTC CCAGACGTATGCCACTCGCATGCTGGTGAAGCCTGGTATCACCGGTCCGTGGCAGGTATC CCAAACATACGCTACCCGTATGCTGGTCAAGCCCGGTATTACCGGCCCCTGGCAGGTGTC CCGTCTGTACGCCACCCGCATGCTGGTCAAGCCCGGCATCACCGGTCCGTGGCAAGTGTC CCCGGTCTATGCGACACGTCTGTTGGTCAAGCCCGGCATCACTGGTCCCTGGCAGGTGTC CCGTGTCTATGCTTCGCGTTTGCTCGTCAAGCCCGGTATTACCGGTCCTTGGCAGGTCTC TTCCCTTTATTCCGCGCGACTGCTGGTTAAGCCTGGTATTACCGGTCCGTGGCAGGTTTC ** * * ** ** * ** ***** ** ** ** ** ** *****.** **
1409 1397 1448 1442 1400 1394 1532
B. B. B. B. B. B. B.
longum adolescentis angulatum infantis lactis gallicum pseudocatenulatum
TGGCAGATCCAACTTGAGTAAGGAAGAATCCGAGTCTTTGGACGTCAGCTATGTGCAGAA CGGCAGATCCAACCTGAGTGAGGAGGAATCCGAGGCTCTGGACGTTACCTACGTGCAGAA AGGCAGATCCAACCTGAGTAAGGAGGAGTCGGAGTCTTTGGATGTCAGCTACGTGCAAAG CGGTCGCTCCGATTTGTCTGCCGAAGAGAGTGAGGCCCTTGATGTGGCTTACGTGCAGAA CGGCCGCAATGACCTCAATGAGGAGGAGAGCCGTCAGCTTGATGTGAGTTATGTGCAGGA CGGCCGCAATGATCTGGATGAGGCAGAAAGCCGCCAGCTTGATGTGTCGTATGTGCAGGA CGGCCGTTCGGATCTTTCGCAGGAGCAGAGCGAATATTTGGACGTTTCCTATATCGAAAA ** .* :. .* * . *.. *.: . . * ** ** ** .* *...
1469 1457 1508 1502 1460 1454 1592
B. B. B. B. B. B. B.
longum adolescentis angulatum infantis lactis gallicum pseudocatenulatum
CTGGTCCATATTGGGAGGCATTGTGCTGCTTTTCCGCACTGTCGGTGCCGTCGTCACTCA TTGGTCCATGTTAGGTGACGTCGTGCTGCTGTTCCGTACTGTCGGCGCCGTCGTAACCCA CTGGTCTGTGTTGGGAGACATAGTGCTGCTTTTCCGCACAGTCGGTGCAGTCATCACTCA CTGGTCGATTATGGGAGACATTGTGCTTCTCTTCCGCACGGTCGGTGCCGTGCTGAGTCA CTGGAGCGTCATGGGTGATGTCGTGTACATTCTGCGCACGATTGGCACAGTGCTGCACCC CTGGAGCGTGATGGGAGACATCGTATACATTCTTCGTACGGTGGGCACGGTGCTGCGGCC TTGGTCCATTGCAGGCGATCTTGCGATTCTCGCAAAAACCGTGCTCGTGGTCTTCCGCGG ***: .* .** *. * * . : .* .. ** .* . ** * .
1529 1517 1568 1562 1520 1514 1652
R6
B. B. B. B. B. B. B.
longum adolescentis angulatum infantis lactis gallicum pseudocatenulatum
TAAGGGTGCATACTGA CAAGGGTGCATACTGA TAAGGGTGCATACTAA CAAGGGAGCGTACTGA GAGTGGCGCTTACTAG AAGCGGCGCGTATTAA CACCGGATCTTACTAA * ** * ** *..
1545 1533 1584 1578 1536 1530 1668
Figure S2. Nucleotide sequence alignment for the rfbP gene in 7 different Bifidobacterium species. From top to bottom: BIFCAT_01253 (ZP_03324464) from B. catenulatum DSM 16992, BIFPSEUDO_0324 (ZP_03742673) from B. pseudocatenulatum DSM 20438, BIFADO_01843 (ZP_02029386) from B. adolescentis L2-32, HMPREF9003_1455 (ZP_07696327) from B. dentium JCVIHMP022, BL0249 (NP_695455) from B. longum subsp. longum NCC2705, BIFBRE_03332 (ZP_06595517) from B. breve DSM 20213 and Blon_2114 (YP_002323554) from B. longum subsp. infantis ATCC 15697. The grey shadows indicate the primer sets (F3R4 and F5R5) that yielded the best results. B. B. B. B. B. B. B.
catenulatum pseudocatenulatum adolescentis dentium longum breve infantis
ATGCTTCCTTCGCAGAGAGAATACCCCCCTGTTTTCGAGGGTGGGGTGGGGATGGTGGGA 60 -------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------AT 2 ----------------------------------------------------------AT 2 ------------------------------------------------------------
B. B. B. B. B. B. B.
catenulatum pseudocatenulatum adolescentis dentium longum breve infantis
GGAGAAGTCATGCAAG--GTGGTTTCCG--CAGCTCTGAGCAGGATTCG--GATCAAGTA ---------ATGCAAG--GCGGTTTCCG--CAGCTCTGAGCAGGGTTCG--GATCAAGTA ---------ATGCAAGGCGTGAATACCGGTGAATCCGGCGCACCAAACGGCGAGCAAGGT ---------ATGCAGGGTGAGGCAATCG---------------AATTCGACGAGCATG-GGAGGAAGAATTCAGTATCATGCCGAAG--GACAGAGGCACAGCAGCAGAGTCGCAGGCA GGAGGAAGAATTCAGTATCATGCCGAAG--GACAGAGGCACAGCAGCAGAGTCGCAGGCA ------------------------------------------------------------
114 45 51 34 60 60
B. B. B. B. B. B. B.
catenulatum pseudocatenulatum adolescentis dentium longum breve infantis
GTGAGAACCCAGAAAAT--CCATATCGTCTCTTCTGACTCTCGTCATCAGGATCAGAAGC GTGAGAACCCAGGAAAT--CCATATCGTCTCTTCTAACTCTCGTCACCAGGATCAGAAGC ATGAGGACTCAAGAGAT--TCATATCGTCTCGTCCAATGCGCATCGACAGGCATACGCGT -CCCGTACGCAGGAGAT--TCATATGATTTCCTCGCATACGAACCGTCAGACATATGCAA ACAAGCACCGCGACCATGGCAATGCCCAAAGTACGGGCGAGCGGGAGCCGGTCCTCGGCT ACAAGCACCGCGACCATGGCAATGCCCAAAGTACGGGTGAGCGGGAGCCGGGCCTTGGCT ------------------------------------------------------------
172 103 109 91 120 120
B. B. B. B. B. B. B.
catenulatum pseudocatenulatum adolescentis dentium longum breve infantis
AGGAGCGGCATCAG-----TCACAAAACAACAGCCCTCAG--TTTGAGCCGATGACGTTT AGGAGCGACATCAG-----TCTCAAGACAACAGCCCTCAG--TTTGAGCCGATGATGTTT CCATGCAGAATCAG-----CGTCTG--CAAAAG----CAG--TTCGAGCCGATGACTTTT AAACTCAGAATCAGGTAT-ATTCCGGCCAACCTTCCACGTCTTTCGAACCAATGACCTTC TCGTTCTCCCCCGTTGCCGAATGTGACATTGCGCCGACATTCTTTGACGGCACCTACTTC TCGTTCTCCCCCGTTGCCGAATGTGACATTGCGCCGACATTCTTTGACGGCACCTACTTC ------------------------------------------------------------
225 156 156 150 180 180
B. B. B. B. B. B. B.
catenulatum pseudocatenulatum adolescentis dentium longum breve infantis
TCGAACACGCCATTTTC---CGTTGGTGAAGATGGAAGACG-CAAGGGCAGGCG--GAGT TCGAACACGCCATTTTC---CGTTGGTGAAGATGGAAAGCG-CAAGGGCAGGCG--GAAT TCCAATACCCCGTTTCC---TGCGGAAAAGGAAGGACATAGGCGCGGCAAGGCT--GAAT TCCAATACGCCGTTCCCGACCGGAGACAAGGGTGGTATGCGGAGTCGGAGGGCAC-GCAA TCCTCTACACCGGACGGCAGCTCCACACATAACGACACCACGAACATACGTCCATTGATG TCCTCTACACCGGACGGCAGCTCCACACATAACGACACCACGAACATACGTCCATTGATG ------------------------------------------------------------
279 210 211 209 240 240
B. B. B. B. B. B. B.
catenulatum pseudocatenulatum adolescentis dentium longum breve infantis
CAGG---TTGTGCCCGC-ATGGCGTTATTTTTTTGTCGCTTGCCTGGTGATGGTCGATCT CAGG---TTGTGCCCGC-ATGGCGTTATTCTTTTGTCGTCTGCTTGGTGATGGTCGATCT CG-------ACTCCCCG-GTGGCGTTTCGCCTTCGTCGCCAGCCTGATCGCCATCGATTT CGCGGATCGGCGCCCACGATGGCGATATGCTTTCGTAAGTTGCATGGTCGTCCTCGACAT GGCCATTCTGTGCCGAA-GTGGCGATATCTCTATAACACGACCTTGGTCGTGCTTGATCT GGCCATTCTGTGCCGAA-GTGGCGATATCTCTATAACACGACCTTGGTCGTGCTTGATCT ---------GTGCCCAA-ATGGCGTTATCTGTACAATACGACCATGGTGATTCTGGATCT . ** .*****:*: *: .: . : * **.* . * ** *
335 266 263 269 299 299 50
B. B. B. B. B. B. B.
catenulatum pseudocatenulatum adolescentis dentium longum breve infantis
TGCGGTCATGCTGATTTCGCTGTCCATCAGTCTTTGGGTCAATGATGCCGCATATAAT-TGCGGTCATGCTGATTTCGCTATCCATCAGTCTTTGGGCCAACGATGCCGCATATAAT-GGCAGTGATGCTGCTTTCGTTGACCGTCAGCCTTGTAATCAACGGTGCTGCTTTCGAT-CGCGGTGATGCTGCTGTCGCTGCTGATCAGCCTGCTGGTCAACGGCGAAGCATACGTC-GCTGATGACCATTGTGGCCACGTACATCGTGTTCCTGCTCCGACCTTTCGCTTATACCTA GCTGATGACCATTGTGTCCACGTACATCGTGTTCCTGCTCCGACCTTTCGCTTATACCTA GCTGATGACCATCATCGCCACCTGCATCGTATTCGCGTGCAATCCAGGAGCATATGCCAA ..* * .* * * .**. * . *.. **:*: .
393 324 321 327 359 359 110
B. B. B. B. B. B. B.
catenulatum pseudocatenulatum adolescentis dentium longum breve infantis
---GCCATCAATGGGGCTATGCCGTTCTGGCTG-------TTCCTCGTCTGCTTCAGTTT ---GCCATCAATGGGGCCATGCCGTTCTGGCTG-------TTCCTCATCTGCTTCAGCCT ---GCCATCGGCGATGTGATGCCGTTTTGGCTT-------TTCCTCATTCTGTTCAGCCT ---ACCATCGTACGATCCATGCCGTTCGAGCTG-------TTCCTGTTCTGCTTCAGCTT TGTGCAGAGCATTGGTCCCGGCGAATACGGCGTTTTTAGCTTCCTGCTGTTGGCCTGCGT TGTGCAGAGCATTGGCCCCGGCGAATACGGCGTTTTTAGCTTCCTGCTGTTGACCTGCGT TGTGCGGAACATGGGACCATCCGATTATGGTGTATTCAGTTTCCTGAGCTTGGCCTGCAT .* .: . . * .:* .* ***** *:* *
443 374 371 377 419 419 170
B. B. B. B. B. B. B.
catenulatum pseudocatenulatum adolescentis dentium longum breve infantis
CATCTGGGTGTTGAGTCTCACTTTTGCTGGAACGTATCATCGCCATGTGATGGCGGAAGG CATCTGGGTGTTGAGCCTCACCTTTGCCGGAACGTATCATCGCCATGTGATGGCGGAAGG GATTTGGTTGCTGTGCTTGGCATTCGCGGGCACATACCACCGCCATGTTATGGCGGACGG GATTTGGGTGCTGTGCCTGGCACTTGCCGGCACGTACCATCGACATCTCATGGCGGATGG ATCGTGGCTCATCAGTCTGCATTCCGCACGCTCCTATGAACGGCACACCATGGGTGAGGG ATCGTGGCTCATCAGTCTGTATTCCGCACGCTCCTATGAACGGCACACCATGGGTGAGGG CTCCTGGCTCATCAGCCTCTATGCAGCTCGAAGCTACGAACGCCACACTATGGGCGAGGG : *** * * :* * . ** *.: ** * ** ** **** ** **
503 434 431 437 479 479 230
B. B. B. B. B. B. B.
catenulatum pseudocatenulatum adolescentis dentium longum breve infantis
CTACGAGCTGTATGCGAAAATCATCAATGCGTCGCTGCTCACCATCATCACTTACTGCTC CTACGAGCTGTATGCGAAAATCATCAACGCGTCGCTGCTCACCATCATCACTTACTGCTC CTATGAGCTGTATGCCAAAATCATCAATGCGTCGTTGCTGACCATCATCTCATACTGCAG TTATGAACTGTATGCGAAGATTATCAATGCTTCGCTGCTCACCGTCCTGCTGTACTGCAG CTATGCCCTGTACGCCAAGCTGTTGAACGCTGCGTTCATCGATTTCATTATGCTGTGCAC CTATGCCCTGTACGCCAAGCTGTTGAACGCTGCGTTCATCGATTTCATTATGCTGTGCAC ATACGGACTATATGCCAAGCTGCTGAATGCGGCCTTTATCGACTTCATTATGCTCTGTAC ** * **.** ** **..* * ** ** * * .* .. **.* : ** :
563 494 491 497 539 539 290
B. B. B. B. B. B. B.
catenulatum pseudocatenulatum adolescentis dentium longum breve infantis
GCTTGCTTTTATTTTCAATCTTTCCTTGCCGCGAACCGCGCTTATCGTCGCTCCGATCAT GCTTGCTTTTATTTTCAATCTTTCCTTGCCGAGAACCGCGCTTATCGTCGCCCCGATCAT CCTTGCATTTATTTTCAAACTTGCATTGCCCCGCACTGCGTTGATTATCGCGCCAGTCAT CCTGTCGTTCACACTCAATCTCGACCTGCCCCGTACCGCACTGATCACGGCGCCGGTCAT GTTGGGGTACCTGTTCCACCTCAATCTTCCCCGGTCGTTGAATGTGTTCATTCCGCTGGT GTTGGGGTACCTGTTCCACCTCAATCTTCCCCGGTCGTTGAATGTGTTCATTCCGCTGGT ATTGGGCTACTTGTTCCATCTGAATGTGCCGCGTTCCCTGAATGTATTTATTCCTATCCT * *: **.* ** . * ** .* :* . : .* . ** * *
623 554 551 557 599 599 350
B. B. B. B. B. B. B.
catenulatum pseudocatenulatum adolescentis dentium longum breve infantis
TGCATTCTTCCTAGAAGTCATCGCCCGTTGGCAGATGCGTCAGTGGCAGCATCGCAGCCG TGCATTCTTCCTAGAAGTCATCGCCCGTTGGCAGATGCGTCAGTGGCAGCATCGCAGCCG CGCCTTCTTCTTGGACATCATCGCACGCTGGCAGATGCGTCAAGCGTTGCATCGTCACCG CGCGTTCTTTCTGGCGGTGATTGAACGTTGGCAGATGCGTATGAGCCTGCATCGCAACCG TTCACTGGTGTTGGTTATTATCGAGCGTTGGATTATGCGCCGCGCGCTGCACCGCAACCG TTCACTGGTATTGGTTATTATCGAGCGTTGGATTATGCGCCGCGCGCTGCACCGCAACCG TTCTCTCATCCTCGTTATCGTTGAACGGTGGCTGATGCGTCGTGCGCTTCACCGCAATCG * * * * * .* .* *. ** ***.: ***** . : ** ** .. **
683 614 611 617 659 659 410
B. B. B. B. B. B. B.
catenulatum pseudocatenulatum adolescentis dentium longum breve infantis
TCAGAAGGGTGCCTGTAAGTATAAGACCGTGGTGATCGGCTCTTCCGACGGTATCAATCG TCAGAAGGGCGCCTGTAAGTATAAGACCGTGGTGATTGGTTCTTCCGACGGTATCAATTG CAGCAAAGGCTCTTGCAAGTACAGAACGGTGGTCATCGGATCTTCCGATGGCATCAACAA TCGGAACGGGCGGTGCAAATACAAGACCGTGCTGGTCGGATCATCTACCGGCATCAATGC CATGAATGGCGAATTCAACTACCCGACTGTGGTTATTGGGTCTCCGGAAGGTATCCACCG CATGAATGGCGAATTCAACTACCCGACTGTGGTTATTGGGTCTCCGGAAGGTATCCACCG AGCAAAAGGCGAGTTCAACTACCCCACTATTATCATTGGCTCTCCGGAGGGCATCAGTAA ** ** * ** ** . ** .* * .* ** **: * .. ** ***..
743 674 671 677 719 719 470
B. B. B. B. B. B. B.
catenulatum pseudocatenulatum adolescentis dentium longum breve infantis
TGCGTTGCGTACCATGCGGGACTACTCCAATTGGGGGTATTCGCCGATTGCCGTGTGTCC TGCGTTGCGCACCATGCGGGACTACTCCAATTGGGGGTATTCGCCGATTGCCGTGTGTCC AGCCTTGCAGACCATGCGCAACTGCATGAATTGGGGGTGCGAACCAGTTGCTGTATGTCC CGCCCTGCGCACCATGGCCGACTATTCCAATTGGGGCTATGAACCGATCGCAGTCTGCCC GACGGTCGAACAATTGCGCCAGTGCCGTGGCCTCGGCTACGCGCCAATTGCGGTGTGCCC GACGGTCGAACAATTGCGCCAGTGCCGTGGCCTCGGCTACGCGCCAATTGCGGTGTGCCC AACGCTGAAGCAGCTCAGGGCATGCCTCAGCCTGGGGTATGCACCCATCGCGGTCTGCCC .* * . .. * . *. .. ** *. ..** .* ** ** ** **
803 734 731 737 779 779 530
B. B. B. B. B. B. B.
catenulatum pseudocatenulatum adolescentis dentium longum breve infantis
GATCGAAGTTGACGGTAATGAGAAAGACGCTTATGTGGTCACGTCGTTCGAGCCTG---GATCGAAGCTGACGACAATGAGAAAGACGCTTATGTGGTCACGTCGTTCGAGCCTG---AATTGAAGCGGATCCAGCTTCAAAAGACGCTTATGTGGTTACCTCATACACACCGG---GATCGAAGCGGATCTGCACGAGCCCGACGCCTACGTGGTCACCTCATTCGTACCGG---AGTGGTTTCAGTTAAGAACGAGGATGACCCGGATTCGGCGCAGCATTTGGTGTCGGTGCC AGTGGCTTCAGTCAAGAACGAGGATGACCCGGATTCGGCGCAGCATTTGGTGTCGGTGCC TGTTGCCTCTGTGTGTGATTCGGATGACCCGAATGCTGCTCAACATCTTGTATCGGTTCC .* * *: . .. . *** * * * .. . : . . * *
859 790 787 793 839 839 590
B. B. B. B. B. B. B.
catenulatum pseudocatenulatum adolescentis dentium longum breve infantis
-----ATCCTTCCATTGAAGGTGCCGACAAACTGAAGGTGATCCCGTTCAATGCCGCTTT -----ATCCTTCCATTGAAGGTGCCGACAAGCTGAAGGTGATTCCGTTCAATGCCGCTTT -----ATGCCAATATCGAAGGCTCCAGCAAACTCAAGGTGATTCCTTTCAACTCGGCATT -----ATCCCAACATTCCCGGTGCCGACAAACTCAAGGTGATTCCGTTCAATTCGGCATT CTTCACTCCTGCCAACGATGCTGAGGCACGACTGAAGGTGTTGCCACTCAACTCGCATTT CTTCACTCCTGCCAACGATGCTGAGGCACGACTGAAGGTGTTGCCACTCAACTCGCATTT GTTTATTCCTGCCAATGATGAAGAAGCGCGGCTCAAGGTGTTGGCGTTGAACTCCCATTT * * . *: . * . . ...** ******:* * * ** * .:**
914 845 842 848 899 899 650
B. B. B. B. B. B. B.
catenulatum pseudocatenulatum adolescentis dentium longum breve infantis
CCCGCGTACGTGCGAAAGCTTGGGTGCGCAGGAAGTGTACGTTGCCGACGTGCTTTCCCG CCCGCGTACGTGCGAAAGCTTGGGTGCGCAGGAAGTGTATGTTGCCGACGTGCTGTCCCG CCCACGTATTTGCGAAAGCCTTGGTGTACAGGAAGTCTATGTGGCTGACGTGCTTTCGCG CCCTCGTACCTGCGAAAGCCTTGGTGCTCAGGAGGTGTACATAGCCGATGTGTTGTCGCG GCCGCAAACCGCGAAGCGTATGAAGGCACGTACCGTATTGGTAGCTGATGTACTTACCCG GCCGCAAACCGCGAAGCGTATGAAGGCACGTACCGTATTGGTCGCTGATGTACTTACCCG GCCTCAGACCGCCAAGCGGATGAAGGTTCGCACTATTCTGATCGCTGATGTGCTGACGCA ** *. * .*..* * .. * *. .. .* : .* ** ** **. * :* *.
974 905 902 908 959 959 710
F3
B. B. B. B. B. B. B.
catenulatum pseudocatenulatum adolescentis dentium longum breve infantis
TGATTCCGAAATGCTGCATGGCATTTCGCTTGCGGTCGAATCCCTTGGCATGGAGCTTGC CGATTCCGAAATGCTGCATGGCATTTCCCTTGCGGTCGAGTCGCTTGGCATGGAGCTTGC TGATTCCGAAATGCTGCATGGTCTTTCTCTTGCGGTGGAGTCGCTGGGTATGGAACTTGC CGATTCCGAAATGCTGCATGGCATGTCCTTGGCCATCGAATCCCTGGGTATGGAATTGGC TGATTCTGAGACCATGCGTACATTGTCCCTGGCCGTGGAATCCATGGGCATTGAATTGGC TGATTCTGAGACCATGCGTACATTGTCCCTGGCCGTGGAATCCATGGGCATTGAATTGGC TGATTCTGAAACCATGCGTACTCTTTCGCTCGCTGTTGAATCTATGGGCATCGAGCTGGC ***** **.* .***.*. * ** * ** .* **.** .* ** ** **. * **
1034 965 962 968 1019 1019 770
B. B. B. B. B. B. B.
catenulatum pseudocatenulatum adolescentis dentium longum breve infantis
GTTGGCCGTGTCTCTGGCAGATGTGAGCGGTCATCGCCTGTACTTGCGTAATACCGCCGA GTTGGCTGTTTCCTTGGCTGACGTGAGCGGCCACCGCCTGTACTTGCGTAATACGGCCGA CTTGGCGGTCTCCCTGGCGGATGTGAGCGGTCACCGTTTGTATCTGCGAAACACCACGGA GTTGTCCGTATCGTTAGCCGACATCAGCGGCCATCGCCTGTACCTACGTAACACCACGGA CCTCACTACCTCTGTGGCTGATTTGAGCGGCGCTGACCTGCATTTCCGCAACGATCCGAC CCTCACTACCTCTGTGGCTGATTTGAGCGGCGCTGACCTGCATTTCCGCAACGATCCGAC TCTTACTGCATCCGTTGCAGATCTGAGCGGCGCAAATCTGCAGCTACACAATGACCCCTC * * . ** * ** ** * ***** . . ** * * *. ** .. * .
1094 1025 1022 1028 1079 1079 830
B. B. B. B. B. B. B.
catenulatum pseudocatenulatum adolescentis dentium longum breve infantis
ACAGCCTGTATTGTTGGCCAGTTTGCCGCAG--TATACGAATACGACGTACGTGATTAAA ACAGCCGGTATTGCTGGCCAGTTTGCCGCAG--TATACGAATACGACGTACGTGATTAAA ACAGCCGGTTCTGTTGGCTTCCCTGCCGCAG--TACACGGGAGCTGCGAACGTAATCAAG ACAGCCCGTGCTGCTGGCCAGTCTGCCGCAA--TACACCAACTCTGCGTATGCGGTCAAA GATGCCAGTAGTCATTGCACGACTGACGCAG--TACTCAACCGTTACCCGCATCCTCAAG GATGCCAGTAGTCACTGCACGACTGACGCAG--TACTCAACCGTTACCCGCATCCTCAAG TATGCCGATTTTGACAGCCCGACTTGCACAGTATTCTCTGCCG--ACTCGCATCTTTAAG .:*** .* * ** * *.**. *: :* . .* . . * **.
1152 1083 1080 1086 1137 1137 888
B. B. B. B. B. B. B.
catenulatum pseudocatenulatum adolescentis dentium longum breve infantis
CGCCTGCTGGATATCGTTGGTTCGGCTTTCGCGCTGCTGATTTTCTCGCCGCTGATGTTG CGCCTGCTGGATATCGTTGGTTCGGCTTTCGCGCTGCTGATTTCCTCGCCGCTGATGTTG CGTTTGCTGGACATCATCGGCTCACTGTGCGCGCTGATTATTTCCTCTCCGATTCTTCTC CGTGTACTTGATATCGTTTGCTCGGCGCTTGCGTTGATCATATCCTCTCCGCTGATGCTG CGTGTGTGCGATATCGTGCTTTCCGGTATCGCCATTATCTTGAGCTCGCCCATCATGCTA CGTGTGTGCGATATCGTGCTTTCCGGTATCGCCATTATCTTGAGCTCGCCCATCATGCTA CGTGTGTGCGATATCGTTCTCTCCCTGGTGGCAATCATTCTGAGTTCGCCCATCATGCTA ** *. ** ***.* ** ** * .* * : ** ** .* .* *
1212 1143 1140 1146 1197 1197 948
B. B. B. B. B. B. B.
catenulatum pseudocatenulatum adolescentis dentium longum breve infantis
GGTACGGCGCTCGCCATCAAGCTTGATGACGGTGGTCCGATTTTCTTCTCGCAGAAGCGT GGTACGGCGATCGCCATCAAGCTTGATGACGGTGGTTCGATTTTCTTCTCGCAGCAGCGT GGCGTCGCCATCGCCATCAAGCTTGATGATGGCGGTCCGGTGTTCTTCTCTCAGACCCGT GGCGTGGCCATTGCCATCAAATTGGATGATGGTGGTCCGGTGTTCTTCTCACAGACACGT TGGGTGGCATACAAGGTCAAGCGGGAAGACGGCGGCCCTGTGTTCTATTCGCAGACGCGC TGGGTGGCATACAAGGTCAAGCGGGAAGACGGCGGCCCTGTGTTCTATTCGCAGACGCGC TGGGCTGCATATAAGGTTAAGCGAGAAGACGGTGGCCCCGTGTTCTACTCACAGACTCGT * . ** : .. .* **. **:** ** ** * .* ****: ** ***.. **
1272 1203 1200 1206 1257 1257 1008
B. B. B. B. B. B. B.
catenulatum pseudocatenulatum adolescentis dentium longum breve infantis
ATCGGTTTGCATGGCAAGCCGTTTACCATGTATAAATTCCGTTCCATGGTTACCAATGCG ATCGGTTTGCATGGCAAGCCGTTTACCATGTATAAATTCCGTTCCATGGTCACCAATGCG ATCGGACTGCACGGCAAGCCGTTCAAAATGTATAAATTCCGTTCCATGGTCACCAATGCT ATCGGATTGCATGGCCGCCCGTTCAAAATGTATAAGTTCCGCTCCATGGTTACCAATGCC ATCGGCATTTACGGCAAGCCGTTTACCATGTACAAGTTCCGTTCCATGCGCACTGATGCC ATCGGCATCTACGGCAAGCCGTTCACCATGTACAAGTTCCGTTCCATGCGTACTGACGCC ATCGGCATCTACGGCAAGCCTTTCACTATGTACAAATTCCGTTCCATGCGGCTGAACGCC ***** * * ***.. ** ** *. ***** **.***** ****** . .* **
F5
1332 1263 1260 1266 1317 1317 1068
B. B. B. B. B. B. B.
catenulatum pseudocatenulatum adolescentis dentium longum breve infantis
GAAGAATTGAAGAAGAAACTTGCCGAAGAAAATGGACAGACCGATCGTTTCATCTTCAAA GAAGAATTGAAGAAGAAACTTGCCGAAGAAAATGGACAGACCGATCGTTTCATCTTCAAA GAGGAGCTGAAGAAGAAACTCGCTGAGGAAACTGGGCAGGAAGACCGGTTCATCTTCAAA GAGGAGTTGAAGAAGAAACTCGCAGAGGAAAATGGCCAGTCCGATCGTTTTATTTTCAAG GACGAGATCAAGGCGAAACTGGCTAAGGAACGCGGCATTGAGGATCGTTTTATCTTCAAG GACGAAATCAAGGCGAAATTGGCTAAGGAACGCGGTATTGAGGATCGTTTTATCTTCAAG GATAAGATGGATGCCGAAGTGGCTGCCGCAGCTGGCGTGGAACTGGGAACAACATTCAAG ** .*. * .* .. .** * ** .. *.* ** : . : * : * *****.
1392 1323 1320 1326 1377 1377 1128
B. B. B. B. B. B. B.
catenulatum pseudocatenulatum adolescentis dentium longum breve infantis
ATGAAGGATGATCCGAGAATTACGAAGGTTGGTCGTTTTATTCGTAAGACTTCGTTGGAT ATGAAGGATGATCCGAGAATTACGAAGGTCGGTCGTTTTATTCGTAAGACTTCGTTGGAT ATGAAAGATGATCCGCGTATCACCAAGGTAGGTCACTTCATCCGTAAGACTTCTTTGGAT ATAAAAGACGATCCGCGCATTACCAAAATCGGTCATTTTATTCGAAAGACCTCACTGGAT CTCAAGGACGATCCACGCGTGACTAAGATCGGTCACTTTATTCGTAAAACATCGCTGGAT CTTAAGGACGATCCACGCGTGACCAAGATTGGTCATTTCATTCGTAAGACGTCGCTGGAC GTCAAGGACGATCCTCGTGTTACTAAAATTGGTAAGTTTATCCGTAAGACTTCCATTGAC * **.** ***** .* .* ** **..* ***.. ** ** **:**.** ** * **
1452 1383 1380 1386 1437 1437 1188
B. B. B. B. B. B. B.
catenulatum pseudocatenulatum adolescentis dentium longum breve infantis
GAATTCCCACAGTTCTTCAACGTTTTGAAGGGCGACATGTCTTTGGTTGGTCCTCGCCCG GAATTCCCCCAGTTCTTCAACGTTTTGAAGGGCGACATGTCTTTGGTTGGTCCTCGCCCG GAGTTCCCGCAGTTCTACAATGTGCTCAAGGGCGATATGTCTCTGGTCGGTCCGCGCCCG GAATTCCCGCAATTCTTCAACGTCTTGAAAGGTGACATGTCGTTGGTAGGGCCGCGCCCA GAGTTCCCGCAGTTCTTTAACGTGTTCAAGGGCGATATGAGCTTGGTTGGGCCTCGCCCG GAATTCCCGCAGTTCTTTAACATGTTCAAGGGCGATATGAGCTTGGTTGGTCCTCGCCCG GAGGTTCCTCAGTTCTTCAATGTGCTGAAGGGCGATATGTCCATGGTGGGTCCGCGTCCG **. * ** **.****: ** .* * **.** ** ***: **** ** ** ** **.
1512 1443 1440 1446 1497 1497 1248
B. B. B. B. B. B. B.
catenulatum pseudocatenulatum adolescentis dentium longum breve infantis
GCTTTGCCGGAAGAGGTTGCCCGCTATGGTTCCCTTTATTCCGCGCGACTGCTGGTTAAG GCTTTGCCGGAAGAGGTTGCTCGCTATGGTTCCCTTTATTCCGCGCGACTGCTGGTTAAG GCATTGCCGGAAGAAGTGGCCCGCTATGGTTCCCTATATTCCGCCCGACTGCTGGTCAAG GCACTGCCTGAGGAAGTGGCACGGTACGGGTCATTATATTCGGCGCGACTGTTGGTCAAG CCGCTGCCTGAAGAAGTCGCGCGATATGACATGCTGTATTCCACGAGACTGCTGGTTAAG CCGCTGCCTGAAGAAGTCGCGCGATATGACATGCTGTACTCCACACGACTGCTGGTTAAG CAACGTCAATATGAGGTTGACCAGTACTCGCCATTGTATTCCACTCGCCTACTGGTCAAG . *. * **.** *. *. ** * ** ** .* .*.**. **** ***
1572 1503 1500 1506 1557 1557 1308
B. B. B. B. B. B. B.
catenulatum pseudocatenulatum adolescentis dentium longum breve infantis
CCTGGTATTGCCGGTCCTTGGCAGGTTTCCGGCCGTTCGGATCTTTCGCAGGAGCAGAGC CCTGGTATTACCGGTCCGTGGCAGGTTTCCGGCCGTTCGGATCTTTCGCAGGAGCAGAGC CCGGGCATCACCGGTCCATGGCAGGTGTCCGGCCGGTCCGATCTTTCACAGGAGCAAAGC CCCGGCATCACTGGACCGTGGCAGGTTTCCGGACGCTCCGATCTTTCCCAAGAGCAAAGT CCTGGTATTACTGGACCGTGGCAGATTTCCGGACGCAGCGATTTGACTCAGGAACAGTCG CCTGGTATTACTGGACCGTGGCAGATTTCCGGACGCAGTGATTTGACTCAGGAGCAGTCG CCGGGTATCACCGGTCCTTGGCAGATTTCCGGGCGTAATGATTTGTCTCAGGAACAGTCT ** ** ** .* **:** ******.* ***** ** : *** * :* **.**.**.:
B. B. B. B. B. B. B.
catenulatum pseudocatenulatum adolescentis dentium longum breve infantis
GAATATCTGGACGTTTCCTATATCGAAAATTGGTCCATTGCAGGCGATCTTGCGATTCTC GAATATTTGGACGTTTCCTATATCGAAAATTGGTCCATTGCAGGCGATCTTGCGATTCTC GAATATTTGGACGTCTCCTACATTGAGAATTGGTCCATCGCTGGAGATCTTGCGATTCTT GAATATTTGGACGTTTCCTATATCGAAAATTGGTCCATTACAGGCGATTTAGCCATTCTT GAGTATGCGGATGTGTCGTATATTCAGGACTGGTCTATCACGGGGGACATCGTGATTCTG GAGTATGCGGATGTGTCGTATATTCAGGACTGGTCTATTACGGGGGACATCGTGATTCTG GAATATGCAGATGTGTCATATATTCAGAATTGGTCTATCACGGGGGATATCGCGATTCTG **.*** .** ** ** ** ** *..* ***** ** .* ** ** * * *****
R4
R5
1632 1563 1560 1566 1617 1617 1368
1692 1623 1620 1626 1677 1677 1428
B. B. B. B. B. B. B.
catenulatum pseudocatenulatum adolescentis B. dentium longum breve infantis
GCAAAAACCGTGCTCGTGGTCTTCCGCGGCACCGGATCTTACTAA GCAAAAACCGTGCTCGTGGTCTTCCGCGGCACCGGATCTTACTAA GCCAAAACCGTGATGGTAATCTTCACTGGCCGTGGATCGTACTGA GCGAAAACAGTTCTAGTGGTATTCCGCGGAACGGGATCATATTAA TTGAAGACTGTGGTGGCAGTGTTTAAGGGGACTGGATCGTACTGA TTGAAGACTGTGGTGGCAGTGTTTAAGGGGACTGGATCGTACTGA CTGAAGACAGTGGGCGCGGTATTTAATGGAACTGGTATGTAG--**.** ** * ..* ** . ** . **:: **
1737 1668 1665 1671 1722 1722 1470
