CLINICAL RESEARCH e-ISSN 1643-3750 © Med Sci Monit, 2015; 21: 371-375 DOI: 10.12659/MSM.893077
Functional Study of -724I/D Polymorphism in Apolipoprotein M (apoM) Gene Promoter Region and its Association with Myocardial Infarction
Received: 2014.11.18 Accepted: 2014.12.04 Published: 2015.01.31
Authors’ Contribution: Study Design A Data Collection B Analysis C Statistical Data Interpretation D Manuscript Preparation E Literature Search F Funds Collection G
ABCEF 1 ABCEG 2 ABCE 3 CEF 4 ACEF 5
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Hua Guo* Xian-Xian Zhao Xiao-Juan Zhang* Wei Chen Jie Zhang
1 Department of Geriatric Cardiology, Jinling Hospital, Nanjing Clinical Medical College, Second Military Medical University, Nanjing, Jiangsu, P.R. China 2 Department of Cardiology, Changhai Hospital, Second Military Medical University, Shanghai, P.R. China 3 Dialysis Center, Jingdu Hospital, Nanjing, Jiangsu, P.R. China 4 Department of Otolaryngology – Head and Neck Surgery, Jinling Hospital, Nanjing, Jiangsu, P.R. China 5 Department of Geriatric Cardiology, Jinling Hospital, Nanjing University, School of Medicine, Nanjing, Jiangsu, P.R. China
* These two authors contributed to this work equally Xian-Xian Zhao, e-mail:
[email protected] Departmental sources
The aim of this study was to detect the function of -724I/D polymorphism in the apolipoprotein M (apoM) gene promoter region and to determine its relationship with myocardial infarction (MI). We selected 309 patients with MI and 309 healthy controls for this case-control study. The PCR products of the apoM gene promoter region were directly sequenced to analyze the -724I/D polymorphism. Differences in frequency distributions of genotype and allele were compared between the MI group and the control group. We used gene recombination and site-directed mutagenesis technique to observe the impact of -724 I/D on transcription activity of apoM gene promoter in vitro. The allele frequency of the -724Del in the MI group was higher than that in the control group (9.5% vs. 3.2%, OR=3.156, 95% CI (1.876~5.309), P