J Physiol 594.17 (2016) pp 5029–5030
5029
Erratum/Corrigendum
Expression of concern With the agreement of the authors, the Editor-in-Chief and the publisher John Wiley & Sons, The Journal of Physiology is publishing four Expressions of Concern regarding four articles that appeared in The Journal between 2009 and 2014.
The Journal of Physiology
The Journal was alerted to the strong resemblance of western blots in figures in each of these four articles to blots in other figures in the same article and/or in other articles. After investigation by journal staff and experts in the field and consultation with the authors, we could not rule out the possibility that the western blots had been duplicated. All the articles involved have overlapping authorship. The similarity of the western blots raises questions about the reliability of some of the data in the four articles. The authors, the Editor-in-Chief and the publisher considered each paper separately before agreeing that publication of an Expression of Concern for each paper was necessary. The Journal has asked the corresponding authors’ institution to investigate these cases. Eduardo R. Ropelle, Jos´e R. Pauli, Dennys E. Cintra, Marisa J. S. Frederico, Ricardo A. De Pinho, L´ıcio A. Velloso & Cl´audio T. De Souza (2009). Acute exercise modulates the Foxo1/PGC-1α pathway in the liver of diet-induced obesity rats. J Physiol. 587(9):2069–76. DOI: 10.1113/jphysiol.2008.164202. The blot used for alpha-tubulin in Figure 2C appears to be repeated in the following articles/figures: Figure 4I, labelled as GLUT4 in the article: Prada PO, Pauli JR, Ropelle ER, Zecchin HG, Carvalheira JB, Velloso LA, Saad MJ (2006). Selective modulation of the CAP/Cbl pathway in the adipose tissue of high fat diet treated rats. FEBS Lett. 580(20):4889–94. Figure 6F, labelled as IP: IR / IB pY in the article: Flores MB, Fernandes MF, Ropelle ER, Faria MC, Ueno M, Velloso LA, Saad MJ, Carvalheira JB (2006). Exercise improves insulin and leptin sensitivity in hypothalamus of Wistar rats. Diabetes 55(9):2554–61. Figure 2B, labelled as Total AMPK in the article: Ropelle ER, Pauli JR, Zecchin KG, Ueno M, de Souza CT, Morari J, Faria MC, Velloso LA, Saad MJ, Carvalheira JB (2007). A central role for neuronal adenosine 5 -monophosphate-activated protein kinase in cancer-induced anorexia. Endocrinology. 148(11):5220–9. Figure 3B, labelled as Total p70S6K in the article: Ropelle ER, Pauli JR, Fernandes MF, Rocco SA, Marin RM, Morari J, Souza KK, Dias MM, Gomes-Marcondes MC, Gontijo JA, Franchini KG, Velloso LA, Saad MJ, Carvalheira JB (2008). A central role for neuronal AMP-activated protein kinase (AMPK) and mammalian target of rapamycin (mTOR) in high-protein diet-induced weight loss. Diabetes 57(3):594–605. Figure 2G, labelled as IB: JAK2, and Figure 2H, labelled as STAT3, in the article: C Ropelle ER1, Fernandes MF, Flores MB, Ueno M, Rocco S, Marin R, Cintra DE, Velloso LA, Franchini KG, Saad MJ, Carvalheira JB (2008). Central exercise action increases the AMPK and mTOR response to leptin. PLoS One 3(12):e3856. DOI: 10.1371/journal.pone.0003856. Figure 1D, labelled as IB: IKKbeta, and Figure 2D, labelled as IRbeta, in the article: C 2016 The Authors. The Journal of Physiology C 2016 The Physiological Society
DOI: 10.1113/JP272727
5030
Erratum/Corrigendum
J Physiol 594.17
´ Flores MB, Rocha GZ, Damas-Souza DM, Osorio-Costa F, Dias MM, Ropelle ER, Camargo JA, de Carvalho RB, Carvalho HF, Saad MJ, Carvalheira JB (2012). Obesity-induced increase in tumor necrosis factor-α leads to development of colon cancer in mice. Gastroenterology Sep;143(3):741–53.e1–4. DOI: 10.1053/j.gastro.2012.05.045. The other papers on which an Expression of Concern has been issued as a result of this investigation are: Eduardo R. Ropelle, Jos´e R. Pauli, Patr´ıcia Prada, Dennys E. Cintra, Guilherme Z. Rocha, Juliana C. Moraes, Marisa J. S. Frederico, Gabrielle da Luz, Ricardo A. Pinho, Jos´e B. C. Carvalheira, Licio A. Velloso, Mario A. Saad & Cl´audio T. De Souza (2009). Inhibition of hypothalamic Foxo1 expression reduced food intake in diet-induced obesity rats. J Physiol, 587(10), pp 2341–2351. DOI 10.1113/jphysiol.2009.170050. Souza Pauli LS, Ropelle EC, de Souza CT, Cintra DE, da Silva AS, de Almeida Rodrigues B, de Moura LP, Marinho R, de Oliveira V, Katashima CK, Pauli JR & Ropelle ER (2014). Exercise training decreases mitogen-activated protein kinase phosphatase-3 expression and suppresses hepatic gluconeogenesis in obese mice. J Physiol, 592(6), 1325–40. DOI: 10.1113/jphysiol.2013.264002. Claudio T. De Souza, MarisaJ.S.Frederico, Gabrielle da Luz, DennysE.Cintra, Eduardo R. Ropelle, Jose R.Pauli, and Lıcio A. Velloso(2010). Acute exercise reduces hepatic glucose production through inhibition of the Foxo1/HNF-4α pathway in insulin resistant mice. J Physiol 588.12, 2239–2253.
C 2016 The Authors. The Journal of Physiology C 2016 The Physiological Society
5029
Erratum/Corrigendum
Expression of concern With the agreement of the authors, the Editor-in-Chief and the publisher John Wiley & Sons, The Journal of Physiology is publishing four Expressions of Concern regarding four articles that appeared in The Journal between 2009 and 2014.
The Journal of Physiology
The Journal was alerted to the strong resemblance of western blots in figures in each of these four articles to blots in other figures in the same article and/or in other articles. After investigation by journal staff and experts in the field and consultation with the authors, we could not rule out the possibility that the western blots had been duplicated. All the articles involved have overlapping authorship. The similarity of the western blots raises questions about the reliability of some of the data in the four articles. The authors, the Editor-in-Chief and the publisher considered each paper separately before agreeing that publication of an Expression of Concern for each paper was necessary. The Journal has asked the corresponding authors’ institution to investigate these cases. Eduardo R. Ropelle, Jos´e R. Pauli, Dennys E. Cintra, Marisa J. S. Frederico, Ricardo A. De Pinho, L´ıcio A. Velloso & Cl´audio T. De Souza (2009). Acute exercise modulates the Foxo1/PGC-1α pathway in the liver of diet-induced obesity rats. J Physiol. 587(9):2069–76. DOI: 10.1113/jphysiol.2008.164202. The blot used for alpha-tubulin in Figure 2C appears to be repeated in the following articles/figures: Figure 4I, labelled as GLUT4 in the article: Prada PO, Pauli JR, Ropelle ER, Zecchin HG, Carvalheira JB, Velloso LA, Saad MJ (2006). Selective modulation of the CAP/Cbl pathway in the adipose tissue of high fat diet treated rats. FEBS Lett. 580(20):4889–94. Figure 6F, labelled as IP: IR / IB pY in the article: Flores MB, Fernandes MF, Ropelle ER, Faria MC, Ueno M, Velloso LA, Saad MJ, Carvalheira JB (2006). Exercise improves insulin and leptin sensitivity in hypothalamus of Wistar rats. Diabetes 55(9):2554–61. Figure 2B, labelled as Total AMPK in the article: Ropelle ER, Pauli JR, Zecchin KG, Ueno M, de Souza CT, Morari J, Faria MC, Velloso LA, Saad MJ, Carvalheira JB (2007). A central role for neuronal adenosine 5 -monophosphate-activated protein kinase in cancer-induced anorexia. Endocrinology. 148(11):5220–9. Figure 3B, labelled as Total p70S6K in the article: Ropelle ER, Pauli JR, Fernandes MF, Rocco SA, Marin RM, Morari J, Souza KK, Dias MM, Gomes-Marcondes MC, Gontijo JA, Franchini KG, Velloso LA, Saad MJ, Carvalheira JB (2008). A central role for neuronal AMP-activated protein kinase (AMPK) and mammalian target of rapamycin (mTOR) in high-protein diet-induced weight loss. Diabetes 57(3):594–605. Figure 2G, labelled as IB: JAK2, and Figure 2H, labelled as STAT3, in the article: C Ropelle ER1, Fernandes MF, Flores MB, Ueno M, Rocco S, Marin R, Cintra DE, Velloso LA, Franchini KG, Saad MJ, Carvalheira JB (2008). Central exercise action increases the AMPK and mTOR response to leptin. PLoS One 3(12):e3856. DOI: 10.1371/journal.pone.0003856. Figure 1D, labelled as IB: IKKbeta, and Figure 2D, labelled as IRbeta, in the article: C 2016 The Authors. The Journal of Physiology C 2016 The Physiological Society
DOI: 10.1113/JP272727
5030
Erratum/Corrigendum
J Physiol 594.17
´ Flores MB, Rocha GZ, Damas-Souza DM, Osorio-Costa F, Dias MM, Ropelle ER, Camargo JA, de Carvalho RB, Carvalho HF, Saad MJ, Carvalheira JB (2012). Obesity-induced increase in tumor necrosis factor-α leads to development of colon cancer in mice. Gastroenterology Sep;143(3):741–53.e1–4. DOI: 10.1053/j.gastro.2012.05.045. The other papers on which an Expression of Concern has been issued as a result of this investigation are: Eduardo R. Ropelle, Jos´e R. Pauli, Patr´ıcia Prada, Dennys E. Cintra, Guilherme Z. Rocha, Juliana C. Moraes, Marisa J. S. Frederico, Gabrielle da Luz, Ricardo A. Pinho, Jos´e B. C. Carvalheira, Licio A. Velloso, Mario A. Saad & Cl´audio T. De Souza (2009). Inhibition of hypothalamic Foxo1 expression reduced food intake in diet-induced obesity rats. J Physiol, 587(10), pp 2341–2351. DOI 10.1113/jphysiol.2009.170050. Souza Pauli LS, Ropelle EC, de Souza CT, Cintra DE, da Silva AS, de Almeida Rodrigues B, de Moura LP, Marinho R, de Oliveira V, Katashima CK, Pauli JR & Ropelle ER (2014). Exercise training decreases mitogen-activated protein kinase phosphatase-3 expression and suppresses hepatic gluconeogenesis in obese mice. J Physiol, 592(6), 1325–40. DOI: 10.1113/jphysiol.2013.264002. Claudio T. De Souza, MarisaJ.S.Frederico, Gabrielle da Luz, DennysE.Cintra, Eduardo R. Ropelle, Jose R.Pauli, and Lıcio A. Velloso(2010). Acute exercise reduces hepatic glucose production through inhibition of the Foxo1/HNF-4α pathway in insulin resistant mice. J Physiol 588.12, 2239–2253.
C 2016 The Authors. The Journal of Physiology C 2016 The Physiological Society
