Original Article
Correlation of matrix metalloproteinase-2 and -9 expression with recurrences in primary spontaneous pneumothorax patients Wen-Chin Chiu1, Yi-Chen Lee2, Yu-Han Su3, Chee-Yin Chai4, Stephen Chu-Sung Hu5,6, Shyng-Shiou F. Yuan3,7, Shah-Hwa Chou1 1
Division of Chest Surgery, Department of Surgery, Kaohsiung Medical University Hospital, Kaohsiung Medical University, Kaohsiung, Taiwan;
2
Department of Anatomy, School of Medicine, College of Medicine, Kaohsiung Medical University, Kaohsiung, Taiwan; 3Translational Research
Center, Department of Medical Research, and Department of Obstetrics and Gynecology, 4Department of Pathology, 5Department of Dermatology, Kaohsiung Medical University Hospital, Kaohsiung Medical University, Kaohsiung, Taiwan; 6Department of Dermatology, 7Graduate Institute of Medicine, College of Medicine, Kaohsiung Medical University, Kaohsiung, Taiwan Contributions: (I) Conception and design: WC Chiu, YC Lee, YH Su, SF Yuan, SH Chou; (II) Administrative support: CY Chai, SF Yuan, SH Chou; (III) Provision of study materials or patients: CY Chai, SH Chou; (IV) Collection and assembly of data: WC Chiu, YH Su, SC Hu; (V) Data analysis and interpretation: WC Chiu, YC Lee, SF Yuan; (VI) Manuscript writing: All authors; (VII) Final approval of manuscript: All authors. Correspondence to: Shyng-Shiou F. Yuan. Translational Research Center, Department of Medical Research, and Department of Obstetrics and Gynecology, Kaohsiung Medical University Hospital, Kaohsiung Medical University, 100 Zihyou 1st Road, San-Ming District, Kaohsiung 80756, Taiwan. Email: [email protected]; Shah-Hwa Chou. Division of Chest Surgery, Department of Surgery, Kaohsiung Medical University Hospital, Kaohsiung Medical University, 100 Zihyou 1st Road, San-Ming District, Kaohsiung 80756, Taiwan. Email: [email protected].
Background: Primary spontaneous pneumothorax (PSP) is a common benign disorder. However, unpredictable recurrence is a major concern for most patients. The aim of the present study was to assess the role of matrix metalloproteinase-2 (MMP-2) and MMP-9 in alveolar macrophages of patients with PSP and its relationship with recurrence. Methods: Ninety-two patients who received needlescopic video-assisted thoracoscopic surgery (NVATS) wedge resection of lung with identifiable blebs for PSP were enrolled for the study. Immunohistochemistry was performed to evaluate the expression of MMP-2 and MMP-9 in lung tissues of patients with PSP. The result was correlated with clinicopathological variables and recurrence rates by the chi-square test. The value of MMP-2 and MMP-9 for overall recurrence was evaluated by univariate and multivariable Cox regression analyses. Results: The MMP-2 and MMP-9 staining was predominantly observed in alveolar macrophages of patients with PSP. We found that MMP-2 (recurrence: P
Correlation of matrix metalloproteinase-2 and -9 expression with recurrences in primary spontaneous pneumothorax patients Wen-Chin Chiu1, Yi-Chen Lee2, Yu-Han Su3, Chee-Yin Chai4, Stephen Chu-Sung Hu5,6, Shyng-Shiou F. Yuan3,7, Shah-Hwa Chou1 1
Division of Chest Surgery, Department of Surgery, Kaohsiung Medical University Hospital, Kaohsiung Medical University, Kaohsiung, Taiwan;
2
Department of Anatomy, School of Medicine, College of Medicine, Kaohsiung Medical University, Kaohsiung, Taiwan; 3Translational Research
Center, Department of Medical Research, and Department of Obstetrics and Gynecology, 4Department of Pathology, 5Department of Dermatology, Kaohsiung Medical University Hospital, Kaohsiung Medical University, Kaohsiung, Taiwan; 6Department of Dermatology, 7Graduate Institute of Medicine, College of Medicine, Kaohsiung Medical University, Kaohsiung, Taiwan Contributions: (I) Conception and design: WC Chiu, YC Lee, YH Su, SF Yuan, SH Chou; (II) Administrative support: CY Chai, SF Yuan, SH Chou; (III) Provision of study materials or patients: CY Chai, SH Chou; (IV) Collection and assembly of data: WC Chiu, YH Su, SC Hu; (V) Data analysis and interpretation: WC Chiu, YC Lee, SF Yuan; (VI) Manuscript writing: All authors; (VII) Final approval of manuscript: All authors. Correspondence to: Shyng-Shiou F. Yuan. Translational Research Center, Department of Medical Research, and Department of Obstetrics and Gynecology, Kaohsiung Medical University Hospital, Kaohsiung Medical University, 100 Zihyou 1st Road, San-Ming District, Kaohsiung 80756, Taiwan. Email: [email protected]; Shah-Hwa Chou. Division of Chest Surgery, Department of Surgery, Kaohsiung Medical University Hospital, Kaohsiung Medical University, 100 Zihyou 1st Road, San-Ming District, Kaohsiung 80756, Taiwan. Email: [email protected].
Background: Primary spontaneous pneumothorax (PSP) is a common benign disorder. However, unpredictable recurrence is a major concern for most patients. The aim of the present study was to assess the role of matrix metalloproteinase-2 (MMP-2) and MMP-9 in alveolar macrophages of patients with PSP and its relationship with recurrence. Methods: Ninety-two patients who received needlescopic video-assisted thoracoscopic surgery (NVATS) wedge resection of lung with identifiable blebs for PSP were enrolled for the study. Immunohistochemistry was performed to evaluate the expression of MMP-2 and MMP-9 in lung tissues of patients with PSP. The result was correlated with clinicopathological variables and recurrence rates by the chi-square test. The value of MMP-2 and MMP-9 for overall recurrence was evaluated by univariate and multivariable Cox regression analyses. Results: The MMP-2 and MMP-9 staining was predominantly observed in alveolar macrophages of patients with PSP. We found that MMP-2 (recurrence: P
