Transl. Stroke Res. DOI 10.1007/s12975-015-0400-3
PROTOCOLS
Correcting for Brain Swelling’s Effects on Infarct Volume Calculation After Middle Cerebral Artery Occlusion in Rats Devin W. McBride 1 & Damon Klebe 1 & Jiping Tang 1 & John H. Zhang 1,2,3
Received: 15 March 2015 / Revised: 12 April 2015 / Accepted: 15 April 2015 # Springer Science+Business Media New York 2015
Abstract Evaluating infarct volume is the primary outcome for experimental ischemic stroke studies and is a major factor in determining translation of a drug into clinical trials. Numerous algorithms are available for evaluating this critical value, but a major limitation of current algorithms is that brain swelling is not appropriately considered. The model by Lin et al. is widely used, but overestimates swelling within the infarction, yielding infarct volumes which do not reflect the true infarct size. Herein, a new infarct volume algorithm is developed to minimize the effects of both peri-infarct and infarct core swelling on infarct volume measurement. 2,3,5-Triphenyl-2H-tetrazolium chloride-stained brain tissue of adult rats subjected to middle cerebral artery occlusion was used for infarct volume analysis. When both peri-infarct swelling and infarction core swelling are removed from infarct volume calculations, such as accomplished by our algorithm, larger infarct volumes are estimated than those of Lin et al.’s algorithm. Furthermore, the infarct volume difference between the two algorithms is the greatest for small infarcts (
PROTOCOLS
Correcting for Brain Swelling’s Effects on Infarct Volume Calculation After Middle Cerebral Artery Occlusion in Rats Devin W. McBride 1 & Damon Klebe 1 & Jiping Tang 1 & John H. Zhang 1,2,3
Received: 15 March 2015 / Revised: 12 April 2015 / Accepted: 15 April 2015 # Springer Science+Business Media New York 2015
Abstract Evaluating infarct volume is the primary outcome for experimental ischemic stroke studies and is a major factor in determining translation of a drug into clinical trials. Numerous algorithms are available for evaluating this critical value, but a major limitation of current algorithms is that brain swelling is not appropriately considered. The model by Lin et al. is widely used, but overestimates swelling within the infarction, yielding infarct volumes which do not reflect the true infarct size. Herein, a new infarct volume algorithm is developed to minimize the effects of both peri-infarct and infarct core swelling on infarct volume measurement. 2,3,5-Triphenyl-2H-tetrazolium chloride-stained brain tissue of adult rats subjected to middle cerebral artery occlusion was used for infarct volume analysis. When both peri-infarct swelling and infarction core swelling are removed from infarct volume calculations, such as accomplished by our algorithm, larger infarct volumes are estimated than those of Lin et al.’s algorithm. Furthermore, the infarct volume difference between the two algorithms is the greatest for small infarcts (
