Nucleic Acids Research, Vol. 18, No. 9 2817

Complete cDNA sequence encoding the B subunit of human factor XilI Ulrich Grundmann, Claudia Nerlich, Thomas Rein and Gerd Zettimeissl Department of Molecular Biology, Research Institutes, Behringwerke AG, Posifach 1140, 3550

Marburg, FRG Submitted March 19, 1990

EMBL accession

protein-glutamine:amine gamma-glutamyltransferase). In blood plasma FXII was described to consist of two catalytic a-subunits and in addition two b-subunits (together MW 320 000). A full length cDNA clone encoding the FXIIIb subunit was isolated from a human liver lambda gtlO library by screening with oligonucleotides generated from the FXIIb encoding cDNA sequence published by Ichinose et al. (2). These authors presented

REFERENCES 1. Bohn,H. (1978) Mol. Cell. Biochem. 20, 67-75. 2. Ichinose,A., McMullen,B.A., Fujikawa,K. and Davie,E.W. (1986) Biochemistry 25, 4633-4638.

70

50

30

X51823

a FXIIIb cDNA sequence lacking the 5'-end of the mRNA and the translational start codon. Our 2613 bp cDNA comprises 420 bp of the 5'-untranslated region (including EcoRI linkers) and encodes the total protein with 661 residues (MW 75 491) including the complete hydrophobic signal sequence (residue -20 to -1).

Here we report on the complete cDNA sequence of the b-subunit of human factor xI (FXIII). FXII is a blood coagulation protein that is distributed both extracellulary and intracellulary (1). Its enzymatic form acts as a transglutaminase and is also called activated fibrin-stabilizing factor or fibrinoligase (EC 2.3.2.13,

10

no.

90

110

TTGCCGAAATGGATTGCCAAAACGGTCACCAGCACATTTCCCAGGAGCTTGAAGTTCTCAGGATCCACATGCAGCTTGTCACAGTGCAGTTCACTCAGCTGGGCAAAGGTGCCCTTGAGA 170

150

130

190

210

230

TCATCCAGGTGCTTTATGGCATCTCCCAAGGAAGTCAGCACCTTCTTGCCATGTGCCTTGACTTTGGGGTTGCCCATGATGGCAGAGGCAGAGGACAGGTTGCCAAAGCTGTCAAAGAAC

310 290 330 270 250 350 CTCTGGGTCCATGGGTAGACAACCAGGAGCCTTCCCAGGGTTTCTCCTCCAGCATCTTCCACATTCACCTTGCCCCACAGGCTTGTGATAGTAGCCTTGTCCTCCTCTGTGAAATGACCC 390 410 430 370 450 470 ATGGCGTCTGGACTAGGAGCTTATTGATAACCTCAGACGTTCCCACCACTGAAGATGAGGTTGAAAAACCTGACTTTTATCATCATATTGATAATCTCAGGAGAACTCTATGCAGAAGAG M

R

L

K

N

L

T

F

I

I

I

L

I

I

S

G

E

L

Y

-20

530

510

490

550

570

A

E

-1

+1

E

590

AAACCCTGTGGTTTTCCTCATGTGGAAAATGGAAGAATTGCCCAATATTACTATACTTTTAAAAGCTTTTACTTTCCAATGAGCATAGACAAAAAATTGTCATTTTTCTGCTTGGCTGGT K

P

G

C

F

P

H

V

E

N

G

R

I

A

Q

Y

Y

Y

T

F

K

S

F

Y

F

P

M

S

I

D

K

K

L

S

F

F

C

L

A

G

670 650 690 710 TATACCACTGAAAGTGGAAGACAAGAAGAGCAAACCACGTGTACAACAGAAGGCTGGTCTCCAGAGCCAAGGTGCTTCAAAAAATGCACTAAGCCTGACCTGAGTAATGGTTACATCTCT

630

610

Y

T

E

T

S

G

R

Q

E

E

Q

T

T

C

T

T

G

W

S

P

E

R

P

770

750

730

E

C

F

K

K

C

790

T

K

P

D

L

S

N

810

G

Y

I

S

830

GATGTAAAGTTATTGTATAAAATTCAAGAGAACATGCATTATGGTTGCGCTTCAGGGTACAAAACCACTGGAGGGAAGGATGAAGAAGTGGTTCAATGTCTCTCTGATGGATGGTCTTCT D

V

L

K

L

Y

K

I

Q

E

N

M

H

Y

G

C

A

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G

Y

K

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T

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E

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S

910 890 930 870 950 CAACCAACCTGTAGGAAAGAACATGAAACGTGTTTGGCTCCTGAATTATATAATGGAAATTATTCCACAACACAGAAAACATTCAAAGTGAAGGACAAAGTACAATACGAATGTGCTACT N Y S T T Q K T F K V K D K V Q Y E C A T Q P T C R K E H E T C L A P E L Y N G 850

1010

990

970

1030

1050

1070

GGCTACTACACAGCTGGAGGAAAGAAGACAGAGGAGGTAGAATGTCTCACATACGGATGGTCTCTCACACCAAAATGTACCAAATTAAAGTGCTCTTCTTTAAGATTAATTGAAAATGGT G

Y

Y

T

A

G

G

K

K

T

E

E

V

E

C

L

T

Y

G

W

S

L

1130

1110

1090

T

P

K

C

T

K

L

1150

K

C

S

S

L

R

L

1170

I

E

N

G

1190

TATTTTCATCCTGTAAAGCAAACCTATGAAGAAGGAGATGTCGTTCAGTTTTTCTGTCATGAAAATTATTATCTAAGTGGATCTGATTTAATTCAATGCTATAACTTTGGTTGGTACCCA Y

F

H

P

V

K

Q

T

Y

E

E

G

D

V

V

Q

F

F

C

H

E

N

1250

1230

1210

Y

Y

L

S

G

S

D

1270

L

I

Q

C

Y

N

F

1290

G

W

Y

P

1310

GAATCTCCTGTATGCGAAGGAAGAAGAAACAGATGTCCTCCTCCACCTCTGCCCATAAACTCCAAAATTCAAACACATTCAACAACTTATCGTCATGGAGAAATAGTTCATATAGAATGT E

S

P

V

C

E

G

R

R

N

R

C

P

P

P

P

P

I

N

S

K

1370

1350

1330

L

I

Q

T

H

S

T

T

1390

Y

R

H

G

E

I

V

H

I

E

C

1430

1410

GAACTTAATTTTGAGATCCATGGGTCAGCAGAAATACGTTGTGAAGATGGAAAATGGACAGAACCTCCAAAATGCATTGAAGGACAGGAGAAGGTAGCCTGTGAGGAACCACCCTTCATT E

L

N

F

E

I

H

G

S

A

E

I

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C

E

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K

W

T

E

P

1490

1470

1450

G

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C

I

E

G

Q

E

K

V

A

C

E

E

1530

1510

P

P

F

I

1550

GAAAATGGTGCAGCAAATTTACACTCTAAGATTTATTACAATGGGGATAAAGTGACATATGCATGTAAAAGCGGCTACCTTCTCCATGGATCGAATGAGATAACTTGTAATCGTGGAAAA E

N

G

A

A

N

L

H

S

K

I

Y

Y

N

G

D

V

T

Y

A

C

1610

1590

1570

K

K

S

G

Y

L

L

H

G

S

N

E

I

T

C

R

G

K

1670

1650

1630

N

TGGACACTTCCTCCTGAGTGTGTTGAAAATAATGAGAATTGTAAGCATCCTCCTGTTGTAATGAATGGGGCTGTTGCAGACGGGATATTGGCAAGCTATGCAACAGGATCCTCAGTGGAA W

T

L

P

P

E

C

V

E

N

N

E

N

C

K

H

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P

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N

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A

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A

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A

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Y

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2818 Nucleic Acids Research, Vol. 18, No. 9 1690 1710 1730 1750 1770 1790 TATAGATGCAATGAATATTACTTACTGAGGGGATCAAAAATATCTCGTTGCGAACAAGGAAAATGGTCATCCCCACCTGTTTGCTTGGAACCATGTACTGTTAATGTGGATTACATGAAC Y

R

C

N

E

Y

Y

L

L

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I

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1810 1830 1850 1870 1890 1910 AGAAATAACATAGAAATGAAGTGGAAATATGAAGGGAAAGTCTTACATGGAGATTTAATAGATTTTGTATGTAAACAGGGATATGACTTATCTCCATTAACCCCATTGTCTGAATTATCT R

N

N

I

E

M

K

W

K

1930

Y

E

G

K

V

L

H

1950

G

D

L

I

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K

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Y

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L

T

P

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L

S

2010

1990

1970

2030 GTGCAGTGCAACAGAGGAGAAGTGAAATATCCTTTATGTACTAGAAAAGAATCTAAAGGAATGTGCACATCTCCTCCTCTTATTAAACATGGAGTCATTATTAGTTCAACAGTAGACACC V

Q

C

N

R

G

E

V

K

2050

Y

P

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T

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2070

E

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T

2130 2150 TATGAAAATGGCTCTTCAGTAGAATACAGATGTTTTGATCACCATTTCCTAGAAGGATCTAGGGAGGCCTATTGTTTAGATGGAATGTGGACTACACCACCATTGTGTTTAGAGCCATGC Y

E

N

G

S

S

V

E

Y

R

2110

2090

C

F

D

H

H

F

L

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L

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2170 2190 2270 ACATTATCTTTTACTGAAATGGAAAAGAATAATTTACTTCTGAAATGGGATTTTGACAACAGACCACACATTTTGCATGGTGAATATATTGAGTTTATTTGTAGAGGAGATACTTATCCA 2210

T

L

S

F

T

E

M

E

K

N

N

L

L

L

K

W

D

2250

2230

F

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Y

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Y

P

2310 2370 2290 2390 GCTGAATTATATATTACTGGATCTATACTTAGAATGCAATGTGACAGAGGGCAGT.IAAAATATCCAAGATGTATTCCAAGACAAAGCACTCTGTCTTATCAAGAACCCTTAAGAACATAG E L Y I T G S I L R M Q C D R G Q L K Y P R C I P R Q S T L S Y Q E P L R T 2330

2350

2450

2470

A

2410

2430

2490

2510

AAATGAATGGCAGAAAGAGGAGTCATATTTCAATACATCATGAAATTCCTTATAAAATATAATTTTGAGGAAATAAGTTAAAAACTTCGGAGTTTTTCCTTGCTTAAATATTTGAATCTA 2570 2590 2530 2550 AATTATTTGTGCTGAACATTTCATTATTTATAAATGAAAACCAATAAATGTCTAATTTTTAGTTTGTAAAAAAAAAAAAAAAAAAAA