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Gastroente~vlogiaJaponica Vol.I0, No. 4.--1975--

--Original Article--

COMPARISON OF DETECTABILITY OF ELEVATED AMYLASE OF S E R U M AND URINE IN PANCREATIC DISEASES BY TWO AMYLASE ASSAY M E T H O D S USING STARCH SUBSTRATES OF DIFFERENT DIGESTIVE RATES TO PANCREATIC AMYLASE T e t s u o H A Y A K A W A , Yasushi T O D A , S a b u r o N A K A Z A W A , Aiji N O D A , F r a n k F. H A L L * a n d Nicholas C. H I G H T O W E R *

The Second Department of Internal Medicine, Nagoya University School of Medicine, Nagoya, Japan *Scott and White Clinic, Temple, Texas, U.S.A. Summary Detectability of abnormally high serum and urine amylases was investigated on patients with pancreatic diseases using amylase assays with substrates of different digestive rates to pancreatic amylase. Ratios of amylase activities determirted by a chromogenic assay usirtg a Remazolbrilliant Blue R starch (RBB assay) to those by Caraway's assay using a Lintner soluble starch (R/C ratio) were calculated on duodenal and salivary amylases obtained from 16 subjects undergoing a pancreozymir~-secretin test. The R/C ratio of the duodenal amylase (M • SD = 0.56 • 0.12) was significantly higher (p