YEAST
VOL. 8: 587-588
(1992)
Corrigendum to: Cloning and Sequencing of the Yeast Saccharomyces cerevisiae SECl gene localized on chromosome IV M . K. AALTO, L. RUOHONEN, K. HOSONO* A N D S. KERANEN? VTT, Biotechnical Laboratory, P.O. Box 202, SF-02151 Espoo, Finland *Fermentation Research Institute, Agency of Industrial Science and Technology, Tsukuba-City, 305 Japan
Received 31 March 1992
In the article 'Cloning and sequencing of the yeast Saccharomyces cerevisiae SECl gene localized on chromosome IV' by M. K. Aalto, L. Ruohonen, K. Hosono and S. Keranen (Yeast 7,643-650, 1991), the nucleotide sequence of the SECI gene of Saccaromyces cerevisiae was reported including 260 bp 3' of the translation stop codon TGA. An open reading frame continues beyond the stop codon and was later found to be highly homologous to asparagine synthetase of several species. This cloning artefact must have been created during the construction of ?Correspondingauthor.
the genomic library, which was obtained by ligating partial Suu3A fragments to the BumHI site on a yeast centromeric plasmid pHR70 (Hans Ronne, personal communication). A piece of noncontiguous DNA had become ligated to the Sau3A site GATC overlapping the stop codon TGA of the SECl open reading frame, which itself remained unaffected. This sequence must represent the asparagine synthetase gene of S. cerevisiae. The correct 3' non-coding sequence of SECl including the TGA stop codon is shown in Figure I , and was obtained by cloning a 1289 bp EcoRI fragment from yeast genomic DNA directly to a
TQATCCCTTA AAGAAGACAG TGATAAAAAR AATCACTTGC TCTTGTCAAA AAAAAAAGAT ATAAACGTAC TATTTATGTA TCGTTCCTAT
90
GTACTATCTA ACATGCTTAT GGAGAAATTC TTGATGATAT TCAGATTTTG TCATATCGTT AATGTATCCG GACGCTGATT TTTTTGTTGC
180
ATGTTCCTTA ATATTTGTCT CTTTGGTTAC TTTATGGCGT CCAAACGCCG TGCCCTTTCT CCACGATCGC CTAGACGAAT TTCCTCCCTT
270
TAATTGTTCA TTTCCTTCAA GCTCTGCTTT ATCTTCCACT TTATTTTTTT GAGTAACCAG CAGCTCTTGC AACGAGTCTT TTTGGTCTTT
360
CTCACTGCGG GTTTCTTGGA GGTTCCCACT CCCCACAACA TCCTGTTTCT CTTCGTTCAT TACTGCCTCC TCTAAAGATT TATCGTTGCT
450
CCTATCTTCT TGCGCACAGT TTGCTCTAAG ATTTTCCTCC TCTTTAAATT TCCTGTATTT TAATGTTGTA TGTCCTGGTA GTAATCTGGC
540
ATGCTCGATG CCTGTTGGTG TATAAGCGGC CGCTTTTGCA CCGCTTCTTG CTTGTAACTG TGGTCTGTGT GATGTGGTCA TGGTCTTGTA
630
GCCCTTTCAA ATGCCAGCCT CTGAGCAAGT TCTTTATGGA GCCTTTTGGA GCTTTGTCAT TCCTATTTCC TTTTCGTGAA GAGATAGACT
710
ATTATTTTlA GTCACACCAA CTTATTTTTC CAGTTGCGAG AAATCCAGAA TTC
763
Figure 1. Sequence of the SECl gene. 0749-503X/92/07058742 $06.00 0 1992 by John Wiley & Sons Ltd
588 small bacterial plasmid pSP73 and identifying the clone by colony hybridization using as a probe a 485 bp EcoRI-XhoI fragment from the 3' end of the SECI coding region. Both strands of the EcoRI fragment were sequenced. The 521 bp preceding the stop codon TGA were identical with the published SECI sequence, confirming that the reported SECI open reading frame was intact. The short stretches of open reading frame 3' to the SECI translation
M. K. AALTO ET AL.
stop codon did not show homology to any known proteins in the EMBL data bank. This cloning artefact was drawn to our attention first by our present collaborator Dr Hans Ronne (Ludwig Institute for Cancer Research, Uppsala Branch, Uppsala, Sweden) and also by Robert Baum (Bethesda, Maryland, U.S.A.). The sequence stored in the EMBL data bank (accession number X62451) has been corrected.
VOL. 8: 587-588
(1992)
Corrigendum to: Cloning and Sequencing of the Yeast Saccharomyces cerevisiae SECl gene localized on chromosome IV M . K. AALTO, L. RUOHONEN, K. HOSONO* A N D S. KERANEN? VTT, Biotechnical Laboratory, P.O. Box 202, SF-02151 Espoo, Finland *Fermentation Research Institute, Agency of Industrial Science and Technology, Tsukuba-City, 305 Japan
Received 31 March 1992
In the article 'Cloning and sequencing of the yeast Saccharomyces cerevisiae SECl gene localized on chromosome IV' by M. K. Aalto, L. Ruohonen, K. Hosono and S. Keranen (Yeast 7,643-650, 1991), the nucleotide sequence of the SECI gene of Saccaromyces cerevisiae was reported including 260 bp 3' of the translation stop codon TGA. An open reading frame continues beyond the stop codon and was later found to be highly homologous to asparagine synthetase of several species. This cloning artefact must have been created during the construction of ?Correspondingauthor.
the genomic library, which was obtained by ligating partial Suu3A fragments to the BumHI site on a yeast centromeric plasmid pHR70 (Hans Ronne, personal communication). A piece of noncontiguous DNA had become ligated to the Sau3A site GATC overlapping the stop codon TGA of the SECl open reading frame, which itself remained unaffected. This sequence must represent the asparagine synthetase gene of S. cerevisiae. The correct 3' non-coding sequence of SECl including the TGA stop codon is shown in Figure I , and was obtained by cloning a 1289 bp EcoRI fragment from yeast genomic DNA directly to a
TQATCCCTTA AAGAAGACAG TGATAAAAAR AATCACTTGC TCTTGTCAAA AAAAAAAGAT ATAAACGTAC TATTTATGTA TCGTTCCTAT
90
GTACTATCTA ACATGCTTAT GGAGAAATTC TTGATGATAT TCAGATTTTG TCATATCGTT AATGTATCCG GACGCTGATT TTTTTGTTGC
180
ATGTTCCTTA ATATTTGTCT CTTTGGTTAC TTTATGGCGT CCAAACGCCG TGCCCTTTCT CCACGATCGC CTAGACGAAT TTCCTCCCTT
270
TAATTGTTCA TTTCCTTCAA GCTCTGCTTT ATCTTCCACT TTATTTTTTT GAGTAACCAG CAGCTCTTGC AACGAGTCTT TTTGGTCTTT
360
CTCACTGCGG GTTTCTTGGA GGTTCCCACT CCCCACAACA TCCTGTTTCT CTTCGTTCAT TACTGCCTCC TCTAAAGATT TATCGTTGCT
450
CCTATCTTCT TGCGCACAGT TTGCTCTAAG ATTTTCCTCC TCTTTAAATT TCCTGTATTT TAATGTTGTA TGTCCTGGTA GTAATCTGGC
540
ATGCTCGATG CCTGTTGGTG TATAAGCGGC CGCTTTTGCA CCGCTTCTTG CTTGTAACTG TGGTCTGTGT GATGTGGTCA TGGTCTTGTA
630
GCCCTTTCAA ATGCCAGCCT CTGAGCAAGT TCTTTATGGA GCCTTTTGGA GCTTTGTCAT TCCTATTTCC TTTTCGTGAA GAGATAGACT
710
ATTATTTTlA GTCACACCAA CTTATTTTTC CAGTTGCGAG AAATCCAGAA TTC
763
Figure 1. Sequence of the SECl gene. 0749-503X/92/07058742 $06.00 0 1992 by John Wiley & Sons Ltd
588 small bacterial plasmid pSP73 and identifying the clone by colony hybridization using as a probe a 485 bp EcoRI-XhoI fragment from the 3' end of the SECI coding region. Both strands of the EcoRI fragment were sequenced. The 521 bp preceding the stop codon TGA were identical with the published SECI sequence, confirming that the reported SECI open reading frame was intact. The short stretches of open reading frame 3' to the SECI translation
M. K. AALTO ET AL.
stop codon did not show homology to any known proteins in the EMBL data bank. This cloning artefact was drawn to our attention first by our present collaborator Dr Hans Ronne (Ludwig Institute for Cancer Research, Uppsala Branch, Uppsala, Sweden) and also by Robert Baum (Bethesda, Maryland, U.S.A.). The sequence stored in the EMBL data bank (accession number X62451) has been corrected.
