ONCOLOGY LETTERS 14: 1795-1801, 2017
Clinical significance of ZNF750 gene expression, a novel tumor suppressor gene, in esophageal squamous cell carcinoma SHO NAMBARA1, TAKAAKI MASUDA1, TARO TOBO2, SHINYA KIDOGAMI1, HISATERU KOMATSU1, KEISHI SUGIMACHI1, HIROSHI SAEKI3, EIJI OKI3, YOSHIHIKO MAEHARA3 and KOSHI MIMORI1 Departments of 1Surgery and 2Pathology, Kyushu University Beppu Hospital, Beppu, Oita 874‑0838; 3 Department of Surgery and Science, Graduate School of Medical Sciences, Kyushu University, Fukuoka, Fukuoka 812‑8582, Japan Received October 31, 2016; Accepted March 28, 2017 DOI: 10.3892/ol.2017.6341 Abstract. The present authors previously identified a novel candidate tumor suppressor gene, zinc finger protein 750 (ZNF750), in esophageal squamous cell carcinoma (ESCC) (1). The present study aimed to clarify the clinical significance of ZNF750 expression in ESCC. The association between ZNF750 DNA mutation status and the mRNA expression was examined by whole exome sequence analysis and quantitative reverse transcription polymerase chain reaction (RT‑qPCR). The expression of ZNF750 in 76 patients with ESCC (Kyushu University Beppu Hospital) was measured using immunohistochemistry and RT‑qPCR. Using this dataset, the association between ZNF750 mRNA expression and clinicopathological factors was examined. Additionally, survival analysis was performed using datasets from the Kyushu University Beppu Hospital and The Cancer Genome Atlas (TCGA). The biological effects of ZNF750 expression were explored using gene set enrichment analysis (GSEA) and were validated using datasets from the Cancer Cell Line Encyclopedia (CCLE) and the Kyushu University Beppu Hospital. ZNF750 expression analyses demonstrated that ZNF750 mRNA expression was lower in patients with the DNA mutations compared with those without the mutations (P
Clinical significance of ZNF750 gene expression, a novel tumor suppressor gene, in esophageal squamous cell carcinoma SHO NAMBARA1, TAKAAKI MASUDA1, TARO TOBO2, SHINYA KIDOGAMI1, HISATERU KOMATSU1, KEISHI SUGIMACHI1, HIROSHI SAEKI3, EIJI OKI3, YOSHIHIKO MAEHARA3 and KOSHI MIMORI1 Departments of 1Surgery and 2Pathology, Kyushu University Beppu Hospital, Beppu, Oita 874‑0838; 3 Department of Surgery and Science, Graduate School of Medical Sciences, Kyushu University, Fukuoka, Fukuoka 812‑8582, Japan Received October 31, 2016; Accepted March 28, 2017 DOI: 10.3892/ol.2017.6341 Abstract. The present authors previously identified a novel candidate tumor suppressor gene, zinc finger protein 750 (ZNF750), in esophageal squamous cell carcinoma (ESCC) (1). The present study aimed to clarify the clinical significance of ZNF750 expression in ESCC. The association between ZNF750 DNA mutation status and the mRNA expression was examined by whole exome sequence analysis and quantitative reverse transcription polymerase chain reaction (RT‑qPCR). The expression of ZNF750 in 76 patients with ESCC (Kyushu University Beppu Hospital) was measured using immunohistochemistry and RT‑qPCR. Using this dataset, the association between ZNF750 mRNA expression and clinicopathological factors was examined. Additionally, survival analysis was performed using datasets from the Kyushu University Beppu Hospital and The Cancer Genome Atlas (TCGA). The biological effects of ZNF750 expression were explored using gene set enrichment analysis (GSEA) and were validated using datasets from the Cancer Cell Line Encyclopedia (CCLE) and the Kyushu University Beppu Hospital. ZNF750 expression analyses demonstrated that ZNF750 mRNA expression was lower in patients with the DNA mutations compared with those without the mutations (P
