J Gastrointest Surg DOI 10.1007/s11605-015-2829-9
ORIGINAL ARTICLE
Circulating Serum Exosomal miRNAs As Potential Biomarkers for Esophageal Adenocarcinoma Karen Chiam 1 & Tingting Wang 1 & David I. Watson 1 & George C. Mayne 1 & Tanya S. Irvine 1 & Tim Bright 1 & Lorelle Smith 1,5 & Imogen A. White 2 & Joanne M. Bowen 3 & Dorothy Keefe 4 & Sarah K. Thompson 5 & Michael E. Jones 6,7 & Damian J. Hussey 1
Received: 20 November 2014 / Accepted: 10 April 2015 # 2015 The Society for Surgery of the Alimentary Tract
Abstract Background The poor prognosis and rising incidence of esophageal adenocarcinoma highlight the need for improved detection methods. The potential for circulating microRNAs (miRNAs) as biomarkers in other cancers has been shown, but circulating miRNAs have not been well characterized in esophageal adenocarcinoma. We investigated whether circulating exosomal miRNAs have potential to discriminate individuals with esophageal adenocarcinoma from healthy controls and non-dysplastic Barrett’s esophagus. Methods Seven hundred fifty-eight miRNAs were profiled in serum circulating exosomes from a cohort of 19 healthy controls, 10 individuals with Barrett’s esophagus, and 18 individuals with locally advanced esophageal adenocarcinoma. MiRNA expression was assessed using all possible permutations of miRNA ratios per individual. Four hundred eight miRNA ratios were differentially expressed in individuals with cancer compared to controls and Barrett’s esophagus (Mann-Whitney U test, P
ORIGINAL ARTICLE
Circulating Serum Exosomal miRNAs As Potential Biomarkers for Esophageal Adenocarcinoma Karen Chiam 1 & Tingting Wang 1 & David I. Watson 1 & George C. Mayne 1 & Tanya S. Irvine 1 & Tim Bright 1 & Lorelle Smith 1,5 & Imogen A. White 2 & Joanne M. Bowen 3 & Dorothy Keefe 4 & Sarah K. Thompson 5 & Michael E. Jones 6,7 & Damian J. Hussey 1
Received: 20 November 2014 / Accepted: 10 April 2015 # 2015 The Society for Surgery of the Alimentary Tract
Abstract Background The poor prognosis and rising incidence of esophageal adenocarcinoma highlight the need for improved detection methods. The potential for circulating microRNAs (miRNAs) as biomarkers in other cancers has been shown, but circulating miRNAs have not been well characterized in esophageal adenocarcinoma. We investigated whether circulating exosomal miRNAs have potential to discriminate individuals with esophageal adenocarcinoma from healthy controls and non-dysplastic Barrett’s esophagus. Methods Seven hundred fifty-eight miRNAs were profiled in serum circulating exosomes from a cohort of 19 healthy controls, 10 individuals with Barrett’s esophagus, and 18 individuals with locally advanced esophageal adenocarcinoma. MiRNA expression was assessed using all possible permutations of miRNA ratios per individual. Four hundred eight miRNA ratios were differentially expressed in individuals with cancer compared to controls and Barrett’s esophagus (Mann-Whitney U test, P
