Rajeev
Gupta,2
abnormalities
M.B.,
B.S.,
Mukesh
ABSTRACT to severe bone
marrow
out
of 152
Cytogenetic protein
difference more
Gupta,3
calorie
using plates
frequent
studies
were
and
in
significant,
in protein
calorie
the
Case
Sex
#{149}
in five
healthy
In the 0.005,
children
controls,
control
group
group implying
malnutrition.
Am.
there
there that
I.
suffering using
were
were
chromosomal
Clin.
Nutr.
from
direct
30:
16
moderate
preparations
of
two
abnormalities
out
of
186.
This
abnormalities
are
1974-1978,
1977.
data has been summarized in Table 1. Three of the five children had associated recent acute chest infections and one had gastroenteritis. All these cases received penicillin (0.4 mega units per day), and one (case 3) received in addition 125 mg of streptomycin daily. The drugs were started only 1 day before the study was conducted. Five healthy children of the same age group served as controls. These were the children coming for routine check up and after a
cases
Clinical type of
Age
DiagWI
HI
g
cm
6000 4000 7000 6000 5500
78 60 78 68 65
nsulnutrltion
‘#{176}
mo
1 2 3 4 5
M M F M F Antibi
30 10 28 12 12 otics
Marasmus Marasmus Marasmus Marasmus Marasmus us ed were
nostic radiation
Materials
and
American
Journal
Infection
#{149}
Bone
marrow .
+
+
-
-
+
+
-
-
-
+
Chest infection Gastroenteritis Chest infection Chest infection Chest infection
Normoblastic Normoblastic Normoblastic Normoblastic Normoblastic
of Clinical
Nutrition
physical examination tions were included
and basic preliminary investigain the study (Table 2). Chromosomal studies were done by direct preparations of bone marrow according to the method described by Tjio and Whang (8), as modified by Solanki and Grover (9). Preparations from all the cases were pooled and a single observer who was not aware of the origin of these examined about 30 metaphase plates from each preparation and scored them for abnormalities such as chromatid breaks, chromatid gaps or isochromosome gaps, chromosomal fragmentation or
‘From the Department of Pediatrics and Pathology, S. P. Medical College and Associated Group of Hospitals, Bikaner, India. 2 Intern in Pediatrics. Present address: Junior Resident, Department of Medicine, Postgraduate Institute of Medical Education and Research, Chandigarh, India. 3Tutor in Pediatrics. 4Reader in Pathology.
methods
The study was conducted in five children suffering from moderate to severe degree of protein calorie malnutrition and in five normal healthy children, their ages ranged from 10 to 30 months. Pertinent clinical
The
Antib
penicil lin and streptomycin.
chemicals, viruses, and specific deficiencies of methionine, cyanocobalamin, and folate have been found to cause chromosomal abnormalities. Perhaps in malnutriton these environmental factors produce chromosomal changes at a lesser threshold. The present study was conducted to find the chromosomal status in subjects of protein calorie malnutrition, the environmental factors have been excluded as far as possible.
1974
out
five
Ramdeo,4M.D.
I
Clinical data of malnourished
a
N.
malnutrition
P
Gupta,2
abnormalities
M.B.,
B.S.,
Mukesh
ABSTRACT to severe bone
marrow
out
of 152
Cytogenetic protein
difference more
Gupta,3
calorie
using plates
frequent
studies
were
and
in
significant,
in protein
calorie
the
Case
Sex
#{149}
in five
healthy
In the 0.005,
children
controls,
control
group
group implying
malnutrition.
Am.
there
there that
I.
suffering using
were
were
chromosomal
Clin.
Nutr.
from
direct
30:
16
moderate
preparations
of
two
abnormalities
out
of
186.
This
abnormalities
are
1974-1978,
1977.
data has been summarized in Table 1. Three of the five children had associated recent acute chest infections and one had gastroenteritis. All these cases received penicillin (0.4 mega units per day), and one (case 3) received in addition 125 mg of streptomycin daily. The drugs were started only 1 day before the study was conducted. Five healthy children of the same age group served as controls. These were the children coming for routine check up and after a
cases
Clinical type of
Age
DiagWI
HI
g
cm
6000 4000 7000 6000 5500
78 60 78 68 65
nsulnutrltion
‘#{176}
mo
1 2 3 4 5
M M F M F Antibi
30 10 28 12 12 otics
Marasmus Marasmus Marasmus Marasmus Marasmus us ed were
nostic radiation
Materials
and
American
Journal
Infection
#{149}
Bone
marrow .
+
+
-
-
+
+
-
-
-
+
Chest infection Gastroenteritis Chest infection Chest infection Chest infection
Normoblastic Normoblastic Normoblastic Normoblastic Normoblastic
of Clinical
Nutrition
physical examination tions were included
and basic preliminary investigain the study (Table 2). Chromosomal studies were done by direct preparations of bone marrow according to the method described by Tjio and Whang (8), as modified by Solanki and Grover (9). Preparations from all the cases were pooled and a single observer who was not aware of the origin of these examined about 30 metaphase plates from each preparation and scored them for abnormalities such as chromatid breaks, chromatid gaps or isochromosome gaps, chromosomal fragmentation or
‘From the Department of Pediatrics and Pathology, S. P. Medical College and Associated Group of Hospitals, Bikaner, India. 2 Intern in Pediatrics. Present address: Junior Resident, Department of Medicine, Postgraduate Institute of Medical Education and Research, Chandigarh, India. 3Tutor in Pediatrics. 4Reader in Pathology.
methods
The study was conducted in five children suffering from moderate to severe degree of protein calorie malnutrition and in five normal healthy children, their ages ranged from 10 to 30 months. Pertinent clinical
The
Antib
penicil lin and streptomycin.
chemicals, viruses, and specific deficiencies of methionine, cyanocobalamin, and folate have been found to cause chromosomal abnormalities. Perhaps in malnutriton these environmental factors produce chromosomal changes at a lesser threshold. The present study was conducted to find the chromosomal status in subjects of protein calorie malnutrition, the environmental factors have been excluded as far as possible.
1974
out
five
Ramdeo,4M.D.
I
Clinical data of malnourished
a
N.
malnutrition
P
