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Chitosan-based hydrogel tissue scaffolds made by 3D plotting promotes osteoblast proliferation and mineralization
This content has been downloaded from IOPscience. Please scroll down to see the full text. 2015 Biomed. Mater. 10 035004 (http://iopscience.iop.org/1748-605X/10/3/035004) View the table of contents for this issue, or go to the journal homepage for more
Download details: IP Address: 165.123.34.86 This content was downloaded on 14/05/2015 at 08:45
Please note that terms and conditions apply.
Biomed. Mater. 10 (2015) 035004
doi:10.1088/1748-6041/10/3/035004
Paper
received
3 February 2015
Chitosan-based hydrogel tissue scaffolds made by 3D plotting promotes osteoblast proliferation and mineralization
re vised
19 March 2015 accep ted for publication
19 March 2015 published
13 May 2015
I-Hsin Liu1, Shih-Hsin Chang2 and Hsin-Yi Lin1 1
Institute of Biochemical and Biomedical Engineering, National Taipei University of Technology, Taipei 106, Taiwan Department of Plastic Surgery, Mackay Memorial Hospital, No. 92, Sec 2, Zhongshan N Rd, Taipei 104, Taiwan
2
E-mail: [email protected] Keywords: 3D plotting, bone repair, chitosan
Abstract A 3D plotting system was used to make chitosan-based tissue scaffolds with interconnected pores using pure chitosan (C) and chitosan cross-linked with pectin (CP) and genipin (CG). A freeze-dried chitosan scaffold (CF/D) was made to compare with C, to observe the effects of structural differences. The fiber size, pore size, porosity, compression strength, swelling ratio, drug release efficacy, and cumulative weight loss of the scaffolds were measured. Osteoblasts were cultured on the scaffolds and their proliferation, type I collagen production, alkaline phosphatase activity, calcium deposition, and morphology were observed. C had a lower swelling ratio, degradation, porosity and drug release efficacy and a higher compressional stiffness and cell proliferation compared to CF/D (p
Search
Collections
Journals
About
Contact us
My IOPscience
Chitosan-based hydrogel tissue scaffolds made by 3D plotting promotes osteoblast proliferation and mineralization
This content has been downloaded from IOPscience. Please scroll down to see the full text. 2015 Biomed. Mater. 10 035004 (http://iopscience.iop.org/1748-605X/10/3/035004) View the table of contents for this issue, or go to the journal homepage for more
Download details: IP Address: 165.123.34.86 This content was downloaded on 14/05/2015 at 08:45
Please note that terms and conditions apply.
Biomed. Mater. 10 (2015) 035004
doi:10.1088/1748-6041/10/3/035004
Paper
received
3 February 2015
Chitosan-based hydrogel tissue scaffolds made by 3D plotting promotes osteoblast proliferation and mineralization
re vised
19 March 2015 accep ted for publication
19 March 2015 published
13 May 2015
I-Hsin Liu1, Shih-Hsin Chang2 and Hsin-Yi Lin1 1
Institute of Biochemical and Biomedical Engineering, National Taipei University of Technology, Taipei 106, Taiwan Department of Plastic Surgery, Mackay Memorial Hospital, No. 92, Sec 2, Zhongshan N Rd, Taipei 104, Taiwan
2
E-mail: [email protected] Keywords: 3D plotting, bone repair, chitosan
Abstract A 3D plotting system was used to make chitosan-based tissue scaffolds with interconnected pores using pure chitosan (C) and chitosan cross-linked with pectin (CP) and genipin (CG). A freeze-dried chitosan scaffold (CF/D) was made to compare with C, to observe the effects of structural differences. The fiber size, pore size, porosity, compression strength, swelling ratio, drug release efficacy, and cumulative weight loss of the scaffolds were measured. Osteoblasts were cultured on the scaffolds and their proliferation, type I collagen production, alkaline phosphatase activity, calcium deposition, and morphology were observed. C had a lower swelling ratio, degradation, porosity and drug release efficacy and a higher compressional stiffness and cell proliferation compared to CF/D (p
