Glycobiology, 2017, vol. 27, no. 3, 275–277 doi: 10.1093/glycob/cww107 Advance Access Publication Date: 15 September 2016 Erratum
Erratum
Chemical characterization and immunomodulatory properties of polysaccharides isolated from probiotic Lactobacillus casei LOCK 0919 S Górska, P Hermanova, J Ciekot, M Schwarzer, D Srutkova, E Brzozowska, H Kozakova, and A Gamian Glycobiology (2016) doi: 10.1093/glycob/cww047 References
After publication of this article (Górska et al. 2016), we noticed that the original version, unfortunately, contained mistakes. We inadvertently inserted the wrong spectra for Figures 1, 3 and 4 and an incorrect version of Table II. Correct versions are provided below. In addition, in the Results (NMR analysis of L919/B) section of the above-mentioned article, after the statement “The structure of the pentasaccharide repeating unit of L919/B, as determined herein, is shown in Figure 4.” we erroneously omitted the following statement: “This structure is virtually identical to that of the polysaccharide L900/3 isolated from L. rhamnosus LOCK 0900 (Górska et al. 2014).” We apologize for the oversight and possible misunderstanding.
7
6
Górska S, Schwarzer M, Jachymek W, Srutkova D, Brzozowska E, Kozakova H, Gamian A. 2014. Distinct immunomodulation of bone marrow derived dendritic cell responses to Lactobacillus plantarum WCFS1 by two different polysaccharides isolated from Lactobacillus rhamnosus LOCK 0900. Appl Environ Microbiol. 80:6506–6516. Górska S, Hermanova P, Ciekot J, Schwarzer M, Srutkova D, Brzozowska E, Kozakova H, Gamian A. 2016. Chemical characterization and immunomodulatory properties of polysaccharides isolated from probiotic Lactobacillus casei LOCK 0919. Glycobiology. doi:10.1093/glycob/ cww047.
5
4
3
2
CC2-H2
60
FC4-H4
70
CC5-H5
BC4-H4
BC5-H5 DC3-H3 AC2-H2
80
60
DC6-H6
AC6-H6 CC6-H6’
EC2-H2
ω1 - 13C (ppm)
FC6-H6
BC6-H6
CC6-H6 BC2-H2 CC3-H3 EC5-H5 FC2-H2
70
EC4-H4 DC5-H5 EC3-H3
BC3-H3
AC4-H4
AC5-H5 FC5-H5 CC4-H4 FC3-H3
80
DC2-H2 DC4-H4
AC3-H3
90
90
CC1-H1
BC1-H1
100
100
EC1-H1 FC1-H1 DC1-H1
AC1-H1
110
110 7
6
5
4
3
2
ω2 - 1H (ppm) Fig. 1. Selected parts of the 1H-13C HSQC nuclear magnetic resonance (NMR) spectrum of L919/A. This figure is available in black and white in print and in color at Glycobiology online.
© The Author 2016. Published by Oxford University Press. This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact
276
Fig. 3. Selected parts of the 1H-13C HSQC nuclear magnetic resonance (NMR) spectrum of L919/B.
Fig 4. The structure of the pentasaccharide repeating unit of L919/B.
S Górska
277
Erratum Table II. 1H and 13C NMR chemical shifts and selected inter-residue connectivities obtained for the anomeric protons of L919/B from Lactobacillus casei LOCK 0919 Sugar residue
A
→6)-β-D-ManpNAc-(1→
B
→3,4)-α-D-Galp-(1→
C
→3)-β-D-GalpNAc-(1→
D
→4,6)-β-D-Glcp-(1→
E
β-D-Glcp-(1→
Chemical shifts 1H, 13C (ppm) H1 C1
H2 C2
H3 C3
H4 C4
H5 C5
H6 C6
H6′
CH3CO
5.019 98.61 4.809 98.22 4.651 103.34 4.456 104.61 4.372 102.51
4.432 53.02 3.802 67.86 3.974 51.51 3.285 72.78 3.261 72.90
3.74 4 71.76 3.954 77.39 3.768 80.34 3.362 75.68 3.374 75.52
3.458 66.83 4.257 75.55 4.021 67.88 3.519 73.80 3.270 69.24
3.261 75.62 4.063 69.91 3.561 74.68 3.388 75.31 3.377 75.88
4.010 69.41 3.682 61.03 3.704 60.31 3.952 64.80 3.628 60.61
3.725
1.932 22.10
1.947 21.91 3.651 3.801
P 2.6 ppm Selected inter-residue NOESY and 3JH,C (HMBC) connectivities from the anomeric protons of the isolated Sugar residue
H-1 δH (ppm)
Connectivity δH (ppm)
Inter-residue atom/residue
Connectivity δC (ppm)
Inter-residue atom/ residue
75.55 53.02 69.91
C-4 of B C-2 of A C-5 of B
77.39 80.34 69.41 64.80
C-3 of B C-3 of C C-6 of A C-6 of D
A
→6)-β-D-ManpNAc-(1→
5.019
4.257
H-4 of B
B
→3,4)-α-D-Galp-(1→
4.809
C D E
→3)-β-D-GalpNAc-(1→ →4,6)-β-D-Glcp-(1→ β-D-Glcp-(1→ P
4.651 4.456 4.372
3.519 4.063 3.682 3.954 3.768 4.010, 3.722
H-4 of D H-5 of B H-6 of B H-3 of B H-3 of C H-6 of A
Spectra were obtained for 2H2O solutions at 25°C, and acetone (δH 2.225, δC 31.05 ppm) was used as an internal reference.
Erratum
Chemical characterization and immunomodulatory properties of polysaccharides isolated from probiotic Lactobacillus casei LOCK 0919 S Górska, P Hermanova, J Ciekot, M Schwarzer, D Srutkova, E Brzozowska, H Kozakova, and A Gamian Glycobiology (2016) doi: 10.1093/glycob/cww047 References
After publication of this article (Górska et al. 2016), we noticed that the original version, unfortunately, contained mistakes. We inadvertently inserted the wrong spectra for Figures 1, 3 and 4 and an incorrect version of Table II. Correct versions are provided below. In addition, in the Results (NMR analysis of L919/B) section of the above-mentioned article, after the statement “The structure of the pentasaccharide repeating unit of L919/B, as determined herein, is shown in Figure 4.” we erroneously omitted the following statement: “This structure is virtually identical to that of the polysaccharide L900/3 isolated from L. rhamnosus LOCK 0900 (Górska et al. 2014).” We apologize for the oversight and possible misunderstanding.
7
6
Górska S, Schwarzer M, Jachymek W, Srutkova D, Brzozowska E, Kozakova H, Gamian A. 2014. Distinct immunomodulation of bone marrow derived dendritic cell responses to Lactobacillus plantarum WCFS1 by two different polysaccharides isolated from Lactobacillus rhamnosus LOCK 0900. Appl Environ Microbiol. 80:6506–6516. Górska S, Hermanova P, Ciekot J, Schwarzer M, Srutkova D, Brzozowska E, Kozakova H, Gamian A. 2016. Chemical characterization and immunomodulatory properties of polysaccharides isolated from probiotic Lactobacillus casei LOCK 0919. Glycobiology. doi:10.1093/glycob/ cww047.
5
4
3
2
CC2-H2
60
FC4-H4
70
CC5-H5
BC4-H4
BC5-H5 DC3-H3 AC2-H2
80
60
DC6-H6
AC6-H6 CC6-H6’
EC2-H2
ω1 - 13C (ppm)
FC6-H6
BC6-H6
CC6-H6 BC2-H2 CC3-H3 EC5-H5 FC2-H2
70
EC4-H4 DC5-H5 EC3-H3
BC3-H3
AC4-H4
AC5-H5 FC5-H5 CC4-H4 FC3-H3
80
DC2-H2 DC4-H4
AC3-H3
90
90
CC1-H1
BC1-H1
100
100
EC1-H1 FC1-H1 DC1-H1
AC1-H1
110
110 7
6
5
4
3
2
ω2 - 1H (ppm) Fig. 1. Selected parts of the 1H-13C HSQC nuclear magnetic resonance (NMR) spectrum of L919/A. This figure is available in black and white in print and in color at Glycobiology online.
© The Author 2016. Published by Oxford University Press. This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact
276
Fig. 3. Selected parts of the 1H-13C HSQC nuclear magnetic resonance (NMR) spectrum of L919/B.
Fig 4. The structure of the pentasaccharide repeating unit of L919/B.
S Górska
277
Erratum Table II. 1H and 13C NMR chemical shifts and selected inter-residue connectivities obtained for the anomeric protons of L919/B from Lactobacillus casei LOCK 0919 Sugar residue
A
→6)-β-D-ManpNAc-(1→
B
→3,4)-α-D-Galp-(1→
C
→3)-β-D-GalpNAc-(1→
D
→4,6)-β-D-Glcp-(1→
E
β-D-Glcp-(1→
Chemical shifts 1H, 13C (ppm) H1 C1
H2 C2
H3 C3
H4 C4
H5 C5
H6 C6
H6′
CH3CO
5.019 98.61 4.809 98.22 4.651 103.34 4.456 104.61 4.372 102.51
4.432 53.02 3.802 67.86 3.974 51.51 3.285 72.78 3.261 72.90
3.74 4 71.76 3.954 77.39 3.768 80.34 3.362 75.68 3.374 75.52
3.458 66.83 4.257 75.55 4.021 67.88 3.519 73.80 3.270 69.24
3.261 75.62 4.063 69.91 3.561 74.68 3.388 75.31 3.377 75.88
4.010 69.41 3.682 61.03 3.704 60.31 3.952 64.80 3.628 60.61
3.725
1.932 22.10
1.947 21.91 3.651 3.801
P 2.6 ppm Selected inter-residue NOESY and 3JH,C (HMBC) connectivities from the anomeric protons of the isolated Sugar residue
H-1 δH (ppm)
Connectivity δH (ppm)
Inter-residue atom/residue
Connectivity δC (ppm)
Inter-residue atom/ residue
75.55 53.02 69.91
C-4 of B C-2 of A C-5 of B
77.39 80.34 69.41 64.80
C-3 of B C-3 of C C-6 of A C-6 of D
A
→6)-β-D-ManpNAc-(1→
5.019
4.257
H-4 of B
B
→3,4)-α-D-Galp-(1→
4.809
C D E
→3)-β-D-GalpNAc-(1→ →4,6)-β-D-Glcp-(1→ β-D-Glcp-(1→ P
4.651 4.456 4.372
3.519 4.063 3.682 3.954 3.768 4.010, 3.722
H-4 of D H-5 of B H-6 of B H-3 of B H-3 of C H-6 of A
Spectra were obtained for 2H2O solutions at 25°C, and acetone (δH 2.225, δC 31.05 ppm) was used as an internal reference.
