This article was downloaded by: [Selcuk Universitesi] On: 30 December 2014, At: 23:41 Publisher: Taylor & Francis Informa Ltd Registered in England and Wales Registered Number: 1072954 Registered office: Mortimer House, 37-41 Mortimer Street, London W1T 3JH, UK
Veterinary Quarterly Publication details, including instructions for authors and subscription information: http://www.tandfonline.com/loi/tveq20
Characterization of the serum metabolic profile 1
of dairy cows with milk fever using H-NMR spectroscopy a
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a
ab
a
a
Yuhang Sun , Chuchu Xu , Changsheng Li , Cheng Xia , Chuang Xu , Ling Wu & Hongyou Zhang
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Department of College of Animal Science and Veterinary Medicine, Heilongjiang BaYi Agriculture University, Daqing 163319, China b
Department of Synergetic Innovation Center of Food Safety and Nutrition, Northeast Agricultural University, Harbin 150030, China Accepted author version posted online: 16 May 2014.Published online: 13 Jun 2014.
Click for updates To cite this article: Yuhang Sun, Chuchu Xu, Changsheng Li, Cheng Xia, Chuang Xu, Ling Wu & Hongyou Zhang (2014) 1
Characterization of the serum metabolic profile of dairy cows with milk fever using H-NMR spectroscopy, Veterinary Quarterly, 34:3, 159-163, DOI: 10.1080/01652176.2014.924642 To link to this article: http://dx.doi.org/10.1080/01652176.2014.924642
PLEASE SCROLL DOWN FOR ARTICLE Taylor & Francis makes every effort to ensure the accuracy of all the information (the “Content”) contained in the publications on our platform. However, Taylor & Francis, our agents, and our licensors make no representations or warranties whatsoever as to the accuracy, completeness, or suitability for any purpose of the Content. Any opinions and views expressed in this publication are the opinions and views of the authors, and are not the views of or endorsed by Taylor & Francis. The accuracy of the Content should not be relied upon and should be independently verified with primary sources of information. Taylor and Francis shall not be liable for any losses, actions, claims, proceedings, demands, costs, expenses, damages, and other liabilities whatsoever or howsoever caused arising directly or indirectly in connection with, in relation to or arising out of the use of the Content. This article may be used for research, teaching, and private study purposes. Any substantial or systematic reproduction, redistribution, reselling, loan, sub-licensing, systematic supply, or distribution in any form to anyone is expressly forbidden. Terms & Conditions of access and use can be found at http:// www.tandfonline.com/page/terms-and-conditions
Veterinary Quarterly, 2014 Vol. 34, No. 3, 159 163, http://dx.doi.org/10.1080/01652176.2014.924642
SHORT COMMUNICATION Characterization of the serum metabolic profile of dairy cows with milk fever using 1H-NMR spectroscopy Yuhang Suna, Chuchu Xua, Changsheng Lia, Cheng Xiaa,b*, Chuang Xua*, Ling Wua and Hongyou Zhanga a
Department of College of Animal Science and Veterinary Medicine, Heilongjiang BaYi Agriculture University, Daqing 163319, China; b Department of Synergetic Innovation Center of Food Safety and Nutrition, Northeast Agricultural University, Harbin 150030, China
Downloaded by [Selcuk Universitesi] at 23:41 30 December 2014
(Received 20 April 2014; accepted 12 May 2014) Background: Milk fever (MF) is a common calcium metabolism disorder in perinatal cows. Currently, information regarding the detailed metabolism in cows suffering from MF is scant. Objective: The purpose was to study the metabolic profiling of serum samples from cows with MF in comparison to control cows, and thereby exploring other underlying pathological mechanisms of this disease. Animals and methods: In the current study, we compared the serum metabolomic profile of dairy cows with MF (n D 8) to that of healthy dairy cows (n D 24) using a 500-MHz digital 1H-nuclear magnetic resonance (1H-NMR) spectrometer. Based on their clinical presentation and serum calcium concentration, cows were assigned either to the control group (no MF symptoms and serum calcium concentration >2.5 mmol/L) or to the MF group (MF symptoms and serum calcium concentration 2.5 mmol/L), and 8 cows were assigned to the MF group (MF symptoms and serum calcium concentration
Veterinary Quarterly Publication details, including instructions for authors and subscription information: http://www.tandfonline.com/loi/tveq20
Characterization of the serum metabolic profile 1
of dairy cows with milk fever using H-NMR spectroscopy a
a
a
ab
a
a
Yuhang Sun , Chuchu Xu , Changsheng Li , Cheng Xia , Chuang Xu , Ling Wu & Hongyou Zhang
a
a
Department of College of Animal Science and Veterinary Medicine, Heilongjiang BaYi Agriculture University, Daqing 163319, China b
Department of Synergetic Innovation Center of Food Safety and Nutrition, Northeast Agricultural University, Harbin 150030, China Accepted author version posted online: 16 May 2014.Published online: 13 Jun 2014.
Click for updates To cite this article: Yuhang Sun, Chuchu Xu, Changsheng Li, Cheng Xia, Chuang Xu, Ling Wu & Hongyou Zhang (2014) 1
Characterization of the serum metabolic profile of dairy cows with milk fever using H-NMR spectroscopy, Veterinary Quarterly, 34:3, 159-163, DOI: 10.1080/01652176.2014.924642 To link to this article: http://dx.doi.org/10.1080/01652176.2014.924642
PLEASE SCROLL DOWN FOR ARTICLE Taylor & Francis makes every effort to ensure the accuracy of all the information (the “Content”) contained in the publications on our platform. However, Taylor & Francis, our agents, and our licensors make no representations or warranties whatsoever as to the accuracy, completeness, or suitability for any purpose of the Content. Any opinions and views expressed in this publication are the opinions and views of the authors, and are not the views of or endorsed by Taylor & Francis. The accuracy of the Content should not be relied upon and should be independently verified with primary sources of information. Taylor and Francis shall not be liable for any losses, actions, claims, proceedings, demands, costs, expenses, damages, and other liabilities whatsoever or howsoever caused arising directly or indirectly in connection with, in relation to or arising out of the use of the Content. This article may be used for research, teaching, and private study purposes. Any substantial or systematic reproduction, redistribution, reselling, loan, sub-licensing, systematic supply, or distribution in any form to anyone is expressly forbidden. Terms & Conditions of access and use can be found at http:// www.tandfonline.com/page/terms-and-conditions
Veterinary Quarterly, 2014 Vol. 34, No. 3, 159 163, http://dx.doi.org/10.1080/01652176.2014.924642
SHORT COMMUNICATION Characterization of the serum metabolic profile of dairy cows with milk fever using 1H-NMR spectroscopy Yuhang Suna, Chuchu Xua, Changsheng Lia, Cheng Xiaa,b*, Chuang Xua*, Ling Wua and Hongyou Zhanga a
Department of College of Animal Science and Veterinary Medicine, Heilongjiang BaYi Agriculture University, Daqing 163319, China; b Department of Synergetic Innovation Center of Food Safety and Nutrition, Northeast Agricultural University, Harbin 150030, China
Downloaded by [Selcuk Universitesi] at 23:41 30 December 2014
(Received 20 April 2014; accepted 12 May 2014) Background: Milk fever (MF) is a common calcium metabolism disorder in perinatal cows. Currently, information regarding the detailed metabolism in cows suffering from MF is scant. Objective: The purpose was to study the metabolic profiling of serum samples from cows with MF in comparison to control cows, and thereby exploring other underlying pathological mechanisms of this disease. Animals and methods: In the current study, we compared the serum metabolomic profile of dairy cows with MF (n D 8) to that of healthy dairy cows (n D 24) using a 500-MHz digital 1H-nuclear magnetic resonance (1H-NMR) spectrometer. Based on their clinical presentation and serum calcium concentration, cows were assigned either to the control group (no MF symptoms and serum calcium concentration >2.5 mmol/L) or to the MF group (MF symptoms and serum calcium concentration 2.5 mmol/L), and 8 cows were assigned to the MF group (MF symptoms and serum calcium concentration
