Accepted Manuscript Characterization of the proton binding sites of extracellular polymeric substances in an anaerobic membrane bioreactor Yi Liu, Sheng Chang, Fantahun M. Defersha PII:
S0043-1354(15)00230-4
DOI:
10.1016/j.watres.2015.04.007
Reference:
WR 11236
To appear in:
Water Research
Received Date: 14 August 2014 Revised Date:
28 March 2015
Accepted Date: 3 April 2015
Please cite this article as: Liu, Y., Chang, S., Defersha, F.M., Characterization of the proton binding sites of extracellular polymeric substances in an anaerobic membrane bioreactor, Water Research (2015), doi: 10.1016/j.watres.2015.04.007. This is a PDF file of an unedited manuscript that has been accepted for publication. As a service to our customers we are providing this early version of the manuscript. The manuscript will undergo copyediting, typesetting, and review of the resulting proof before it is published in its final form. Please note that during the production process errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain.
ACCEPTED MANUSCRIPT
+
+
H COOH/COO
H 4
H
NH /NH
HPO / PO
H
+
+
2-
-
+
3
4
2
+
+
EPS
+
H
H
RI PT
H +
H
+
H
+
+
H
pKa
14 12 10 8 6 4 2
EP
0
Intensity
TE D
pKa values & intensities (10-4 mole/gEPS)
16
M AN U
SC
H
AC C
Carboxyl
Phosphate
Amine
ACCEPTED MANUSCRIPT
1 2
Characterization of the proton binding sites of extracellular polymeric substances in an anaerobic membrane bioreactor
3
RI PT
4 5
Yi Liu, Sheng Chang*, Fantahun M. Defersha
7
School of Engineering, University of Guelph, Guelph, Ontario, N1G 2W1, Canada,
SC
6
M AN U
8
9
10
TE D
11
*Corresponding Author:
13
Sheng Chang
14
Email: [email protected]
15
Tel: 1-519-824-4120 x 56619
16
Fax: 1-519-836-0227
AC C
17
EP
12
18
19
1
ACCEPTED MANUSCRIPT
Abstract
21
This paper focuses on the characterization of the chemical compositions and acidic constants of
22
the extracellular microbial substances (EPSs) in an anaerobic membrane bioreactor treating
23
synthetic brewery wastewater by using chemical analysis, linear programming analysis (LPA) of
24
titration data, and FT-IR analysis. The linear programming analysis of titration data revealed that
25
the EPSs have proton binding sites with pKa values from pKa ≤ 6, between 6 to 7, and
26
approximately 9.8. The strong acidic sites (pKa ≤ 6) and some weak acidic sites (7.5
S0043-1354(15)00230-4
DOI:
10.1016/j.watres.2015.04.007
Reference:
WR 11236
To appear in:
Water Research
Received Date: 14 August 2014 Revised Date:
28 March 2015
Accepted Date: 3 April 2015
Please cite this article as: Liu, Y., Chang, S., Defersha, F.M., Characterization of the proton binding sites of extracellular polymeric substances in an anaerobic membrane bioreactor, Water Research (2015), doi: 10.1016/j.watres.2015.04.007. This is a PDF file of an unedited manuscript that has been accepted for publication. As a service to our customers we are providing this early version of the manuscript. The manuscript will undergo copyediting, typesetting, and review of the resulting proof before it is published in its final form. Please note that during the production process errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain.
ACCEPTED MANUSCRIPT
+
+
H COOH/COO
H 4
H
NH /NH
HPO / PO
H
+
+
2-
-
+
3
4
2
+
+
EPS
+
H
H
RI PT
H +
H
+
H
+
+
H
pKa
14 12 10 8 6 4 2
EP
0
Intensity
TE D
pKa values & intensities (10-4 mole/gEPS)
16
M AN U
SC
H
AC C
Carboxyl
Phosphate
Amine
ACCEPTED MANUSCRIPT
1 2
Characterization of the proton binding sites of extracellular polymeric substances in an anaerobic membrane bioreactor
3
RI PT
4 5
Yi Liu, Sheng Chang*, Fantahun M. Defersha
7
School of Engineering, University of Guelph, Guelph, Ontario, N1G 2W1, Canada,
SC
6
M AN U
8
9
10
TE D
11
*Corresponding Author:
13
Sheng Chang
14
Email: [email protected]
15
Tel: 1-519-824-4120 x 56619
16
Fax: 1-519-836-0227
AC C
17
EP
12
18
19
1
ACCEPTED MANUSCRIPT
Abstract
21
This paper focuses on the characterization of the chemical compositions and acidic constants of
22
the extracellular microbial substances (EPSs) in an anaerobic membrane bioreactor treating
23
synthetic brewery wastewater by using chemical analysis, linear programming analysis (LPA) of
24
titration data, and FT-IR analysis. The linear programming analysis of titration data revealed that
25
the EPSs have proton binding sites with pKa values from pKa ≤ 6, between 6 to 7, and
26
approximately 9.8. The strong acidic sites (pKa ≤ 6) and some weak acidic sites (7.5
