Journal of Medical Microbiology Papers in Press. Published February 13, 2015 as doi:10.1099/jmm.0.000040
Journal of Medical Microbiology Characterization of Siphoviridae phage Z and studying its efficacy against multi-drug resistant (MDR) Klebsiella pneumoniae planktonic cells and biofilm --Manuscript Draft-Manuscript Number:
Characterization of Siphoviridae phage Z and studying its efficacy against multi-drug resistant (MDR) Klebsiella pneumoniae planktonic cells and biofilm
Phage Z against multi-drug resistant (MDR) Klebsiella pneumoniae
Muhsin Jamal Tahir Hussain Chythanya Rajanna Das Saadia Andleeb
Biofilm is involved in many serious consequences for public health and is a major virulence factor contributing to the chronicity of infections. The aim of the current study was to isolate and characterize a bacteriophage that inhibit multi-drug resistant Klebsiella pneumonia (M) in palnktonic form as well as biofilm. This phage designated as bacteriophage Z was isolated from waste water. Its adsorption rate to its host bacterium was significantly enhanced by MgCl2 and CaCl2. It has a wide range of pH and heat stability. From its one step growth, latent time and burst size was determined that were 24 min and about 320 virions per cell, respectively. As analyzed by transmission electron microscopy, phage Z had a head of width (76±10 nm) and length of (92±14 nm) with an icosahedrons' sides of 38 nm with a non- contractile (200±15 nm) long and (14-29 nm) wide tail belonging to family Siphoviridae of order Caudovirales. Six structural proteins ranging from 18 to 65 kDa were revealed by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE). Its genome was found to be comprised of double stranded DNA with approximate size of 36 kb. Bacteria were grown in suspension and as biofilms to compare the susceptibility of both phenotypes to the phage lytic action. Phage Z was effective in reducing biofilm biomass after 24 h and 48 h and showed more than 2-fold and 3-fold reductions, respectively. Biofilm cells and stationary phase planktonic bacteria were killed at a lower rate than the log-phase planktonic bacteria.
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Characterization of Siphoviridae phage Z and studying its efficacy against multi-drug
resistant (MDR) Klebsiella pneumoniae planktonic cells and biofilm
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Phage Z against multi-drug resistant (MDR) Klebsiella pneumoniae
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Deconex) under biofilm and planktonic conditions.
Methods: A total of 85 strains of K
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