BIOLOGY

OF REPRODUCTION

44,

108-120

(1991)

Characterization

of Feline

MARIE-JOELLE

Conceptus

D. THATCHER,3 FULLER

VICTOR

BAZER,4

W.

Proteins

M. SHILLE,2’3

WANDA

SISUM,3

Gainesville,

MARiA

and

College of Veterinary Medicine,3 Large and Animal Science Department,4

during

Florida

FILOMENA

STACY

Animal University

Pregnancy1 FLISS,4

RANDAL3

Clinical Sciences of Florida

32610

ABSTRACT study

This

characterized

plantation

blastocysts);

metrium);

and

Medium.

Essential

PAGE

appeared

fCP5,

17,

19,

21,

36,

39,

and

50

(n

=

novo

M,

ICP3a,

p1 5.0-5.8.

on Days

39-50,

CA synthesized

on

15,

on

Day

Western

ZG

blotting

transferrin. were

93,

96,

acid

The and

CA

NFI2-terminal

possibly

The

between

at the

detected

first

37

in all media

was

kP5

fCP1

During

and

fCP3a by

ZG

acids,

when

tested

b, and

on

Days

fetoprotein,

and human

rabbit

The with

M,

ftP4, ZG on

and

as alpha

fCP2 amino

units/mI

plasma identities feline

Days

fCP4

16-25, were

16-36 REP

CA

as albumin,

and

porcine

of fCP4 fibroblast

and

by

and

cells

by 2D-

identified fCP2,

p1 5.0-6.0; CA on

produced

and

fCP3a

media

p1 5.0-5.3;

56000,

=

endo-

in Minimal

proteins

20000,

=

M,

preina-

and

and Day

30;

by blastocysts on

Days and

30-39. ftP3b

conceptus

fCP5

have

infected

with

as RBP2

not

been

vesicular

virus.

the

periimplantation of various

period,

species

endometrium

are known

In domestic cats, hormone-dependent proteins of endometrial origin have been studied. Boomsma and Verhage [9] detected a P4-dependent protein (Mr = 30 000) with p1

and

to produce

an

array of proteins such as uteroferrin [1], ovine trophoblast protein (oTP1) [2], bovine trophoblast protein (bTP1) [3], retinol-binding protein (RBP) [4, 5]. These

and

made

10-15,

in culture

radiolabeled

I (fCP1),

p1 5.9-6.3;

Days

fCP2,

was

NH,-terminal