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Cerebrospinal fluid immunocytology in primary central nervous system lymphoma SIR,-In primary central
nervous system (CNS) lymphomas, located within the brain parenchyma, defmitive diagnosis generally requires brain biopsy, and immunological subtyping is largely restricted to those antigens that remain detectable in formalin-fixed paraffin-embedded tissue.1 In four consecutive cases of primary CNS lymphoma, we reached precise diagnosis by immunocytochemical multimarker analysis of cerebrospinal fluid (CSF) cells attached electrostatically to multispot slides coated with poly-L-lysine (PLL).2 These cases all had low-grade pleocytosis (10-86/ml), only a minute fraction of in 4 of 7 malignant cells (0’02-4’4% of all CSF cells,
Cerebrospinal fluid immunocytology in primary central nervous system lymphoma SIR,-In primary central
nervous system (CNS) lymphomas, located within the brain parenchyma, defmitive diagnosis generally requires brain biopsy, and immunological subtyping is largely restricted to those antigens that remain detectable in formalin-fixed paraffin-embedded tissue.1 In four consecutive cases of primary CNS lymphoma, we reached precise diagnosis by immunocytochemical multimarker analysis of cerebrospinal fluid (CSF) cells attached electrostatically to multispot slides coated with poly-L-lysine (PLL).2 These cases all had low-grade pleocytosis (10-86/ml), only a minute fraction of in 4 of 7 malignant cells (0’02-4’4% of all CSF cells,
