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ARD Online First, published on July 23, 2014 as 10.1136/annrheumdis-2013-205138 Basic and translational research
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CD226 (DNAM-1) is associated with susceptibility to juvenile idiopathic arthritis T H C M Reinards,1 H M Albers,1 D M C Brinkman,2 S S M Kamphuis,3 M A J van Rossum,4 H J Girschick,5 C Wouters,6 E P A H Hoppenreijs,7 R K Saurenmann,8 A Hinks,9 J A Ellis,10,11 E Bakker,12 W Verduijn,13 P Slagboom,14 T W J Huizinga,15 R E M Toes,15 J J Houwing-Duistermaat,16 R ten Cate,1 M W Schilham17 Handling editor Tore K Kvien ▸ Additional material is published online only. To view please visit the journal online (http://dx.doi.org/10.1136/ annrheumdis-2013-205138). For numbered affiliations see end of article. Correspondence to Dr Marco W Schilham, Department of Pediatrics, P3-P, Leiden University Medical Center, PO Box 9600, 2300RC, Leiden, The Netherlands; [email protected] THCMR and HMA shared first authorship. RtC and MWS shared last authorship. Received 23 December 2013 Revised 30 April 2014 Accepted 11 July 2014
ABSTRACT Objectives Juvenile idiopathic arthritis ( JIA) is considered a complex genetic autoimmune disease. We investigated the association of genetic variants previously implicated in JIA, autoimmunity and/or immunoregulation, with susceptibility to JIA. Methods A genetic association study was performed in 639 JIA patients and 1613 healthy controls of northwest European descent. Ninety-three single nucleotide polymorphisms (SNP) were genotyped in a candidate gene approach. Results of the entire JIA patient group (all subtypes) were compared with results obtained, alternatively, with a clinically homogeneous patient group including only oligoarticular and rheumatoid factor (RF) negative polyarticular JIA patients (n=493). Metaanalyses were performed for all SNPs that have been typed in other Caucasian JIA cohorts before. Results SNPs in or near PTPN22, VTCN1, the IL2-IL21 region, ANKRD55 and TNFA were confirmed to be associated with JIA ( p
ARD Online First, published on July 23, 2014 as 10.1136/annrheumdis-2013-205138 Basic and translational research
EXTENDED REPORT
CD226 (DNAM-1) is associated with susceptibility to juvenile idiopathic arthritis T H C M Reinards,1 H M Albers,1 D M C Brinkman,2 S S M Kamphuis,3 M A J van Rossum,4 H J Girschick,5 C Wouters,6 E P A H Hoppenreijs,7 R K Saurenmann,8 A Hinks,9 J A Ellis,10,11 E Bakker,12 W Verduijn,13 P Slagboom,14 T W J Huizinga,15 R E M Toes,15 J J Houwing-Duistermaat,16 R ten Cate,1 M W Schilham17 Handling editor Tore K Kvien ▸ Additional material is published online only. To view please visit the journal online (http://dx.doi.org/10.1136/ annrheumdis-2013-205138). For numbered affiliations see end of article. Correspondence to Dr Marco W Schilham, Department of Pediatrics, P3-P, Leiden University Medical Center, PO Box 9600, 2300RC, Leiden, The Netherlands; [email protected] THCMR and HMA shared first authorship. RtC and MWS shared last authorship. Received 23 December 2013 Revised 30 April 2014 Accepted 11 July 2014
ABSTRACT Objectives Juvenile idiopathic arthritis ( JIA) is considered a complex genetic autoimmune disease. We investigated the association of genetic variants previously implicated in JIA, autoimmunity and/or immunoregulation, with susceptibility to JIA. Methods A genetic association study was performed in 639 JIA patients and 1613 healthy controls of northwest European descent. Ninety-three single nucleotide polymorphisms (SNP) were genotyped in a candidate gene approach. Results of the entire JIA patient group (all subtypes) were compared with results obtained, alternatively, with a clinically homogeneous patient group including only oligoarticular and rheumatoid factor (RF) negative polyarticular JIA patients (n=493). Metaanalyses were performed for all SNPs that have been typed in other Caucasian JIA cohorts before. Results SNPs in or near PTPN22, VTCN1, the IL2-IL21 region, ANKRD55 and TNFA were confirmed to be associated with JIA ( p
