682132 case-report2016
SCO0010.1177/2050313X16682132SAGE Open Medical Case ReportsCarter-Wicker et al.
SAGE Open Medical Case Reports
Case Report
SAGE Open Medical Case Reports Volume 4: 1–3 © The Author(s) 2016 Reprints and permissions: sagepub.co.uk/journalsPermissions.nav DOI: 10.1177/2050313X16682132 journals.sagepub.com/home/sco
Can trichomoniasis cause pharyngitis? A case report Kitty Carter-Wicker, Onameyore Utuama and Folashade Omole
Abstract Objectives: We report on a heterosexual male with sore throat diagnosed as oral Trichomoniasis vaginalis. Methods: A rapid strep test and throat culture were negative. An oropharyngeal swab tested positive for Trichomonas vaginalis using a nucleic acid amplification test (NAAT). Results and Conclusion: A detailed sexual history and oropharyngeal swab testing enabled the diagnosis of oral Trichomoniasis vaginalis in a male patient reporting oral sex with a recently infected female. The patient was successfully treated with metronidazole 2 g orally. Keywords Trichomoniasis, sore throat, oral sex, male Date received: 2 August 2016; accepted: 27 October 2016
Introduction Three to five million new cases of trichomoniasis occur annually in the United States.1 Despite a higher prevalence than non-viral sexually transmitted infections (STIs), gonorrhea and chlamydia,2 attention to diagnosis and management in males is lacking. Genital trichomoniasis is more commonly studied, diagnosed and managed, but oropharyngeal infections are much less considered, mainly because the confusion still exists as to whether Trichomonas vaginalis (T. vaginalis) is responsible for pharyngitis and whether it can be isolated from the oropharynx. Here, we discuss a rare occurrence of T. vaginalis isolated from the pharynx. We also emphasize the importance of taking a very detailed sexual history which includes the practice of oral sex and treatment of trichomoniasis in males.
Case presentation Patient is a 30-year-old single, heterosexual male who presented to the Family Medicine clinic with a 5-day history of sore throat and a painful right-sided neck mass; he denied cough, sneezing, rhinorrhea, fever and exposure to tuberculosis. Further investigation revealed participation in unprotected oral sex with his partner for 1 year, who had recently been diagnosed with vaginal trichomoniasis and was prescribed metronidazole. Patient presented with an enlarged right anterior cervical lymph node, measuring 4.5 cm × 3 cm, and was non-tender at the time of the examination; patient states that he had
been taking acetaminophen for the pain. Examination of the oral mucosa revealed good oral hygiene, with pharyngeal and right tonsillar exudates. Rapid streptococcal test and throat culture were negative for group A Streptococcus. Pharyngeal and tonsillar exudates using a Gen-probe Aptima Unisex Swab Kit (blue swab) tested positive for T. vaginalis by nucleic acid amplification test (NAAT). Screening was negative for HIV, syphilis, chlamydia, gonorrhea and tuberculosis. Our patient’s symptoms resolved with oral metronidazole 2 g. We are reporting this case because there is no known case of oral infection from T. vaginalis reported in the literature.
Discussion Trichomoniasis is an STI caused by the protozoan T. vaginalis. As an STI, it is of public health importance because of its association with HIV and contribution to preterm lowbirth-weight babies among infected pregnant women.1 If untreated, it has a propensity to cause infertility through Department of Family Medicine, Morehouse School of Medicine, Atlanta, GA, USA Corresponding Author: Kitty Carter-Wicker, Department of Family Medicine, Morehouse School of Medicine, 1513 East Cleveland Avenue, Bldg 100, East Point, Atlanta, GA 30344, USA. Email: [email protected]
Creative Commons Non Commercial CC-BY-NC: This article is distributed under the terms of the Creative Commons AttributionNonCommercial 3.0 License (http://www.creativecommons.org/licenses/by-nc/3.0/) which permits non-commercial use, reproduction and distribution of the work without further permission provided the original work is attributed as specified on the SAGE and Open Access pages (https://us.sagepub.com/en-us/nam/open-access-at-sage).
2 pelvic inflammatory disease in women and epididymitis in men. Despite a high prevalence of 174 million cases worldwide,1,3 T. vaginalis remains a non-reportable STI. The Centers for Disease Control and Prevention (CDC) recognizes the importance to treat male sexual partners, whether symptomatic or not, with oral metronidazole when female partners test positive.4 T. vaginalis is usually underdiagnosed because about 70% of affected patients remain asymptomatic. Males are more disadvantaged as they are less often symptomatic and diagnosis is less accurate. Diagnosis of T. vaginalis in men requires testing two or more sites and culture sensitivity is less when compared to women.5 The use of NAAT results in higher accuracy among infected males than cultures.5 To increase sensitivity in males, samples from multi sources are recommended: urethral, urine and semen.5 Our patient presented with complaints of sore throat and not as a contact of T. vaginalis. Persistent symptoms following streptococcal pharyngeal standard treatment coincided with a self-report of oral sex with a girlfriend who was recently diagnosed with trichomoniasis. Being male, and reporting pharyngeal symptoms following oral sex with a positive sexual contact, posed a diagnostic challenge. Experimental research has reported the low sensitivity of culture in diagnosing males when compared to polymerase chain reaction (PCR). PCR testing of the oropharynx for Trichomonas is not approved by the US Food although Drug Administration (FDA), and it has a higher sensitivity and specificity using DNA sequencing.6 Take note that the Aptima Trichomonas vaginalis assay is an in vitro qualitative NAAT cleared by the FDA in females for the detection of ribosomal RNA (rRNA) from T. vaginalis to aid in the diagnosis of trichomoniasis.7 We obtained only pharyngeal and tonsillar samples using the Gen-probe Aptima Unisex Swab Kit (blue swab). This kit has been utilized by microbiological researchers and the Colorado Department of Public Health and Environment to assess the accuracy of extra-urogenital (pharyngeal and rectal) testing in trichomoniasis, gonorrhea and chlamydia infections.8,9 A urine sample was not collected, though it might have provided additional information. Although comments from the manufacturer suggest that the Gen-probe can be used to diagnose oral trichomoniasis, diagnosis of oral trichomoniasis via Gen-probe was not found in the literature, and regulatory bodies like the FDA have not recommended swab use in the oropharynx or rectum. Studies from Munson et al.9 recommend extra-urogenital testing in males for diagnostic accuracy. As an oral commensal, Trichomonas tenax (T. tenax) has a lower level of gene expression than T. vaginalis, and experimental introduction of T. tenax in urogenital samples did not affect assay specificity, yet cross-reaction is a possibility.8 This case highlights the importance of thinking broadly with clinical presentations. There are currently no recommendations on obtaining oral samples for T. vaginalis
SAGE Open Medical Case Reports testing. The CDC states that oral sites of infection have not been detected and recommends against testing oral sites. Their conclusion is similar to the National Health Service (NHS) health link on trichomoniasis in the United Kingdom (UK) where they state that it cannot be transmitted by oral sex.10 With our patient’s experience being different from the CDC recommendation, further research is needed to determine whether T. vaginalis is responsible for some false-positive pharyngitis, especially given the rarity of infections outside the genital tract. Duboucher et al.11 report recovering T. vaginalis from pulmonary secretions by utilizing rRNA gene amplification and sequencing which supports our case that T. vaginalis can be transmitted orally. Other forms of sexual intercourse, oral and anal, are practiced by males and females irrespective of their sexual preference. A study on the prevalence of rectal trichomoniasis in men with proctitis revealed no positive test but was 12% for rectal Mycoplasma genitalium infection.10 Although oropharyngeal cultures are not recommended in most STIs, scientists and clinicians understand the importance of testing in eradication of infections. Our case brings to light that T. vaginalis can be transmitted orally and, if a patient presents with a sore throat and a history of unprotected oral sex with a partner who was recently diagnosed with Trichomonas. This warrants testing the oropharynx along with urine or semen for T. to exclude commensals. Declaration of conflicting interests The author(s) declared no potential conflicts of interest with respect to the research, authorship and/or publication of this article.
Ethical approval Our institution does not require ethical approval for reporting individual cases or case series.
Funding The author(s) received no financial support for the research, authorship and/or publication of this article.
Informed consent Written informed consent was obtained from the patient(s) for their anonymized information to be published in this article.
References 1. Sutton M, Sternberg M, Koumans EH, et al. The prevalence of Trichomonas vaginalis infection among reproductive-age women in the United States, 2001–2004. Clin Infect Dis 2007; 45(10): 1319–1326. 2. Marcia MH, Dana ML, Lisa FL, et al. Methods for detection of Trichomonas vaginalis in the male partners of infected women: implications for control of trichomoniasis. J Clin Microbiol 2006; 44(11): 3994–3999. 3. Suzanna CF, Charlotte KK, Jeffrey DK, et al. Prevalence of rectal Trichomonas vaginalis and Mycoplasma genitalium in
Carter-Wicker et al. male patients at the San Francisco STD clinic, 2005–2006. Sex Transm Dis 2008; 35(9): 797–800. 4. Division of STD Prevention, National Center for HIV/AIDS, Viral Hepatitis, STD, and TB Prevention and Centers for Disease Control and Prevention. 2015 Sexually transmitted diseases treatment guidelines, http://www.cdc.gov/std/tg2015/ trichomoniasis.htm 5. Seña AC, Miller WC, Hobbs MM, et al. Trichomonas vaginalis infection in male sexual partners: implications for diagnosis, treatment, and prevention. Clin Infect Dis 2007; 44(1): 13–22. 6. Wendel KA and Workowski KA. Trichomoniasis: challenges to appropriate management. Clin Infect Dis 2007; 44(Suppl. 3): S123–S129.
3 7. http://www.mplnet.com/tests/trichomonas-vaginalis-1 8. http://www.nhs.uk/Conditions/Trichomonas_vaginalis/Pages/ Introduction.aspx 9. Munson E, Wenten D, Phipps P, et al. Retrospective assessment of transcription-mediated amplification-based screening for Trichomonas vaginalis in male sexually transmitted infection clinic patients. J Clin Microbiol 2013; 51(6): 1855–1860. 10. http://www.nhs.uk/Conditions/Trichomonasvaginalis/Pages/ Introduction.aspx 11. Duboucher C, Pierce R, Capron M, et al. Recent advances in pulmonary trichomonosis. Trends Parasitol 2008; 24(5): 201–202.
SCO0010.1177/2050313X16682132SAGE Open Medical Case ReportsCarter-Wicker et al.
SAGE Open Medical Case Reports
Case Report
SAGE Open Medical Case Reports Volume 4: 1–3 © The Author(s) 2016 Reprints and permissions: sagepub.co.uk/journalsPermissions.nav DOI: 10.1177/2050313X16682132 journals.sagepub.com/home/sco
Can trichomoniasis cause pharyngitis? A case report Kitty Carter-Wicker, Onameyore Utuama and Folashade Omole
Abstract Objectives: We report on a heterosexual male with sore throat diagnosed as oral Trichomoniasis vaginalis. Methods: A rapid strep test and throat culture were negative. An oropharyngeal swab tested positive for Trichomonas vaginalis using a nucleic acid amplification test (NAAT). Results and Conclusion: A detailed sexual history and oropharyngeal swab testing enabled the diagnosis of oral Trichomoniasis vaginalis in a male patient reporting oral sex with a recently infected female. The patient was successfully treated with metronidazole 2 g orally. Keywords Trichomoniasis, sore throat, oral sex, male Date received: 2 August 2016; accepted: 27 October 2016
Introduction Three to five million new cases of trichomoniasis occur annually in the United States.1 Despite a higher prevalence than non-viral sexually transmitted infections (STIs), gonorrhea and chlamydia,2 attention to diagnosis and management in males is lacking. Genital trichomoniasis is more commonly studied, diagnosed and managed, but oropharyngeal infections are much less considered, mainly because the confusion still exists as to whether Trichomonas vaginalis (T. vaginalis) is responsible for pharyngitis and whether it can be isolated from the oropharynx. Here, we discuss a rare occurrence of T. vaginalis isolated from the pharynx. We also emphasize the importance of taking a very detailed sexual history which includes the practice of oral sex and treatment of trichomoniasis in males.
Case presentation Patient is a 30-year-old single, heterosexual male who presented to the Family Medicine clinic with a 5-day history of sore throat and a painful right-sided neck mass; he denied cough, sneezing, rhinorrhea, fever and exposure to tuberculosis. Further investigation revealed participation in unprotected oral sex with his partner for 1 year, who had recently been diagnosed with vaginal trichomoniasis and was prescribed metronidazole. Patient presented with an enlarged right anterior cervical lymph node, measuring 4.5 cm × 3 cm, and was non-tender at the time of the examination; patient states that he had
been taking acetaminophen for the pain. Examination of the oral mucosa revealed good oral hygiene, with pharyngeal and right tonsillar exudates. Rapid streptococcal test and throat culture were negative for group A Streptococcus. Pharyngeal and tonsillar exudates using a Gen-probe Aptima Unisex Swab Kit (blue swab) tested positive for T. vaginalis by nucleic acid amplification test (NAAT). Screening was negative for HIV, syphilis, chlamydia, gonorrhea and tuberculosis. Our patient’s symptoms resolved with oral metronidazole 2 g. We are reporting this case because there is no known case of oral infection from T. vaginalis reported in the literature.
Discussion Trichomoniasis is an STI caused by the protozoan T. vaginalis. As an STI, it is of public health importance because of its association with HIV and contribution to preterm lowbirth-weight babies among infected pregnant women.1 If untreated, it has a propensity to cause infertility through Department of Family Medicine, Morehouse School of Medicine, Atlanta, GA, USA Corresponding Author: Kitty Carter-Wicker, Department of Family Medicine, Morehouse School of Medicine, 1513 East Cleveland Avenue, Bldg 100, East Point, Atlanta, GA 30344, USA. Email: [email protected]
Creative Commons Non Commercial CC-BY-NC: This article is distributed under the terms of the Creative Commons AttributionNonCommercial 3.0 License (http://www.creativecommons.org/licenses/by-nc/3.0/) which permits non-commercial use, reproduction and distribution of the work without further permission provided the original work is attributed as specified on the SAGE and Open Access pages (https://us.sagepub.com/en-us/nam/open-access-at-sage).
2 pelvic inflammatory disease in women and epididymitis in men. Despite a high prevalence of 174 million cases worldwide,1,3 T. vaginalis remains a non-reportable STI. The Centers for Disease Control and Prevention (CDC) recognizes the importance to treat male sexual partners, whether symptomatic or not, with oral metronidazole when female partners test positive.4 T. vaginalis is usually underdiagnosed because about 70% of affected patients remain asymptomatic. Males are more disadvantaged as they are less often symptomatic and diagnosis is less accurate. Diagnosis of T. vaginalis in men requires testing two or more sites and culture sensitivity is less when compared to women.5 The use of NAAT results in higher accuracy among infected males than cultures.5 To increase sensitivity in males, samples from multi sources are recommended: urethral, urine and semen.5 Our patient presented with complaints of sore throat and not as a contact of T. vaginalis. Persistent symptoms following streptococcal pharyngeal standard treatment coincided with a self-report of oral sex with a girlfriend who was recently diagnosed with trichomoniasis. Being male, and reporting pharyngeal symptoms following oral sex with a positive sexual contact, posed a diagnostic challenge. Experimental research has reported the low sensitivity of culture in diagnosing males when compared to polymerase chain reaction (PCR). PCR testing of the oropharynx for Trichomonas is not approved by the US Food although Drug Administration (FDA), and it has a higher sensitivity and specificity using DNA sequencing.6 Take note that the Aptima Trichomonas vaginalis assay is an in vitro qualitative NAAT cleared by the FDA in females for the detection of ribosomal RNA (rRNA) from T. vaginalis to aid in the diagnosis of trichomoniasis.7 We obtained only pharyngeal and tonsillar samples using the Gen-probe Aptima Unisex Swab Kit (blue swab). This kit has been utilized by microbiological researchers and the Colorado Department of Public Health and Environment to assess the accuracy of extra-urogenital (pharyngeal and rectal) testing in trichomoniasis, gonorrhea and chlamydia infections.8,9 A urine sample was not collected, though it might have provided additional information. Although comments from the manufacturer suggest that the Gen-probe can be used to diagnose oral trichomoniasis, diagnosis of oral trichomoniasis via Gen-probe was not found in the literature, and regulatory bodies like the FDA have not recommended swab use in the oropharynx or rectum. Studies from Munson et al.9 recommend extra-urogenital testing in males for diagnostic accuracy. As an oral commensal, Trichomonas tenax (T. tenax) has a lower level of gene expression than T. vaginalis, and experimental introduction of T. tenax in urogenital samples did not affect assay specificity, yet cross-reaction is a possibility.8 This case highlights the importance of thinking broadly with clinical presentations. There are currently no recommendations on obtaining oral samples for T. vaginalis
SAGE Open Medical Case Reports testing. The CDC states that oral sites of infection have not been detected and recommends against testing oral sites. Their conclusion is similar to the National Health Service (NHS) health link on trichomoniasis in the United Kingdom (UK) where they state that it cannot be transmitted by oral sex.10 With our patient’s experience being different from the CDC recommendation, further research is needed to determine whether T. vaginalis is responsible for some false-positive pharyngitis, especially given the rarity of infections outside the genital tract. Duboucher et al.11 report recovering T. vaginalis from pulmonary secretions by utilizing rRNA gene amplification and sequencing which supports our case that T. vaginalis can be transmitted orally. Other forms of sexual intercourse, oral and anal, are practiced by males and females irrespective of their sexual preference. A study on the prevalence of rectal trichomoniasis in men with proctitis revealed no positive test but was 12% for rectal Mycoplasma genitalium infection.10 Although oropharyngeal cultures are not recommended in most STIs, scientists and clinicians understand the importance of testing in eradication of infections. Our case brings to light that T. vaginalis can be transmitted orally and, if a patient presents with a sore throat and a history of unprotected oral sex with a partner who was recently diagnosed with Trichomonas. This warrants testing the oropharynx along with urine or semen for T. to exclude commensals. Declaration of conflicting interests The author(s) declared no potential conflicts of interest with respect to the research, authorship and/or publication of this article.
Ethical approval Our institution does not require ethical approval for reporting individual cases or case series.
Funding The author(s) received no financial support for the research, authorship and/or publication of this article.
Informed consent Written informed consent was obtained from the patient(s) for their anonymized information to be published in this article.
References 1. Sutton M, Sternberg M, Koumans EH, et al. The prevalence of Trichomonas vaginalis infection among reproductive-age women in the United States, 2001–2004. Clin Infect Dis 2007; 45(10): 1319–1326. 2. Marcia MH, Dana ML, Lisa FL, et al. Methods for detection of Trichomonas vaginalis in the male partners of infected women: implications for control of trichomoniasis. J Clin Microbiol 2006; 44(11): 3994–3999. 3. Suzanna CF, Charlotte KK, Jeffrey DK, et al. Prevalence of rectal Trichomonas vaginalis and Mycoplasma genitalium in
Carter-Wicker et al. male patients at the San Francisco STD clinic, 2005–2006. Sex Transm Dis 2008; 35(9): 797–800. 4. Division of STD Prevention, National Center for HIV/AIDS, Viral Hepatitis, STD, and TB Prevention and Centers for Disease Control and Prevention. 2015 Sexually transmitted diseases treatment guidelines, http://www.cdc.gov/std/tg2015/ trichomoniasis.htm 5. Seña AC, Miller WC, Hobbs MM, et al. Trichomonas vaginalis infection in male sexual partners: implications for diagnosis, treatment, and prevention. Clin Infect Dis 2007; 44(1): 13–22. 6. Wendel KA and Workowski KA. Trichomoniasis: challenges to appropriate management. Clin Infect Dis 2007; 44(Suppl. 3): S123–S129.
3 7. http://www.mplnet.com/tests/trichomonas-vaginalis-1 8. http://www.nhs.uk/Conditions/Trichomonas_vaginalis/Pages/ Introduction.aspx 9. Munson E, Wenten D, Phipps P, et al. Retrospective assessment of transcription-mediated amplification-based screening for Trichomonas vaginalis in male sexually transmitted infection clinic patients. J Clin Microbiol 2013; 51(6): 1855–1860. 10. http://www.nhs.uk/Conditions/Trichomonasvaginalis/Pages/ Introduction.aspx 11. Duboucher C, Pierce R, Capron M, et al. Recent advances in pulmonary trichomonosis. Trends Parasitol 2008; 24(5): 201–202.
