EDITORIAL
Cardiovascular Research (2014) 101, 185–186 doi:10.1093/cvr/cvt340
Calcium-dependent potassium channels in the heart: clarity and confusion Stanley Nattel* and Xiao Yan Qi Department of Medicine and Research Center, Montreal Heart Institute and Universite´ de Montre´al, 5000 Belanger Street E, Montreal, Quebec, Canada H1 T 1C8 Online publish-ahead-of-print 2 January 2014
This editorial refers to ‘Critical roles of a small conductance Ca21-activated K1 channel (SK3) in the repolarization process of atrial myocytes’ by X.-D. Zhang et al., pp. 317–325, this issue and ‘Overexpression of KCNN3 results in sudden cardiac death’ by S. Mahida et al., pp. 326–334, this issue.
The opinions expressed in this article are not necessarily those of the Editors of Cardiovascular Research or of the European Society of Cardiology.
* Corresponding author. Tel: +1 514 376 3330; fax: +1 514 593 2493, Email: [email protected] Published on behalf of the European Society of Cardiology. All rights reserved. & The Author 2014. For permissions please email: [email protected].
Downloaded from by guest on November 15, 2015
Small-conductance Ca2+-activated K+ (SK) channels are widely expressed throughout the body. After initial dismissal of a functional role for SK channels in the heart,1 a series of elegant studies from the Chiamvimonvat laboratory rekindled interest,2,3 as did subsequent work suggesting that tachypaced rabbit pulmonary veins have enhanced SK channel trafficking to the cell membrane that accelerates repolarization.4 The field really got a boost from GWAS evidence, suggesting that variants in the KCNN3 gene encoding SK isoform 3 (SK3) channels are associated with the risk of atrial fibrillation (AF) in man.5 Questions remain, however, about the functional role that SK currents play in cardiac electrophysiology. Recent studies of SK channels in native tissues have provided conflicting results. Two investigations using apamin to block SK currents showed increased action potential duration (APD) of atrial cells,6,7 while one did not observe an effect.8 Recent detailed studies with a novel selective SK channel blocker provide insight into this variability, by showing that the expression of SK current in the canine atrium is regionally determined and increased by AF-related remodelling, such that statistically non-significant APD effects of SK channel block are seen in control left-atrium, with larger statistically significant effects in pulmonary vein cardiomyocyte sleeves and still larger effects in the presence of AF-related remodelling.9 There are also discrepant findings regarding the effects of SK blockade on atrial arrhythmias: several studies have shown clear anti-arrhyhmic actions,9 – 11 while one paper reported pro-arrhythmic effects.6 Experiments in genetically engineered mouse models have the potential to provide information about the functional importance of ion-channel subunits without the concerns about specificity that plague pharmacological studies. Li et al.12 took advantage of SK2 knockout mice to show a significant role of mice in murine atrial repolarization, as well as enhanced susceptibility of such mice to AF induction. In the present issue, two groups of investigators exploit genetically engineered mice to gain insights into the role of SK3 channels in cardiac electrical function.13,14 Both groups use SK3 overexpression to study
effects on electrophysiology and survival; in addition, Zhang et al.13 use an inducible genetic knockdown system to suppress native SK-3 expression; the Zhang et al.13 study focuses primarily on atrial properties and the Mahida study on ventricular consequences.14 Zhang et al.13 show that SK3 overexpression abbreviates atrial APD and facilitates AF induction, consistent with the majority of pharmacological studies of the role of atrial SK current.7,9 – 11 Genetic SK knockdown had inconsistent effects: APD was not significantly altered, pointing to no significant role of the SK3 subunit in repolarization; however, the apamin effect to prolong APD seen in wild-type and SK3 overexpressing mice was abolished by SK3 knockdown, suggesting a contribution.13 Zhang et al. found substantial premature mortality in SK3 overexpressing mice. The Mahida study complements and extends that of Zhang et al. Heart block and bradyarrhythmias were shown to occur in SK3 overexpressing mice at the time of premature death.14 While at 1 month SK3 overexpressing mice showed inducible atrial arrhythmias and a reduced AV nodal refractory period, at 3 months AV nodal refractoriness tended to be slightly prolonged. Moderate ventricular conduction slowing and increased ventricular APD dispersion were seen in 1-month mice, with enlargement and disorganization of the AV node at 5 months. These studies clarify some questions but raise new ones about the functional role of SK subunits in the heart. At the atrial level, the findings support the idea that SK channels contribute to atrial repolarization and that loss of SK function promotes AF susceptibility. At the ventricular and AV nodal level, the results are quite thought provoking and challenging. Why does SK3 overexpression promote premature death? The simplest explanation of the observations is that SK3 overexpression causes conduction disturbances and bradycardic death. However, it is not clear why increasing repolarizing K+-current should slow conduction. Furthermore, ventricular conduction slowing was modest and AV nodal function was not significantly affected. Could disturbed AV nodal structure have affected AV nodal function enough to cause highgrade block and bradycardic death? AV nodal histology was examined in 5-month-old mice, whereas premature death began at 30 days. It would have been interesting to know what happened to AV node histology at an earlier time-point. The disrupted AV nodal histology is in itself quite interesting: could SK3 overexpression have produced this by disturbing function in some cell-type other than adult cardiomyocytes (e.g. fibroblasts, cardiomyocyte precursors, leucocytes, neural
186 elements)? Finally, could bradycardia have been a result of the terminal event, rather than the cause? SK3 overexpression causes abnormal central control of respiration in mice15: could the deaths have been respiratory in origin, with bradycardia/AV block an agonal cardiac response? Clearly, we need to learn much more about the role of SK channel function in the cardiovascular system. As Alice said in Wonderland: ‘Curioser and curioser’! Conflict of interest: none declared.
Funding Canadian Institutes of Health Research (grants 6957, 44365, 68929); Heart and Stroke Foundation of Canada.
References 1. Eisner DA, Vaughan-Jones RD. Do calcium-activated potassium channels exist in the heart? Cell Calcium 1983;4:371 –386. 2. Xu Y, Tuteja D, Zhang Z, Xu D, Zhang Y, Rodriguez J et al. Molecular identification and functional roles of a Ca2+-activated K+ channel in human and mouse hearts. J Biol Chem 2003;278:49085 –49094. 3. Tuteja D, Xu D, Timofeyev V, Lu L, Sharma D, Zhang Z et al. Differential expression of small-conductance Ca2+-activated K+ channels SK1, SK2, and SK3 in mouse atrial and ventricular myocytes. Am J Physiol Heart Circ Physiol 2005;289:H2714 –H2723. 4. Ozgen N, Dun W, Sosunov EA, Anyukhovsky EP, Hirose M, Duffy HS et al. Early electrical remodeling in rabbit pulmonary vein results from trafficking of intracellular sk2 channels to membrane sites. Cardiovasc Res 2007;75:758–769.
Editorial
5. Ellinor PT, Lunetta KL, Glazer NL, Pfeufer A, Alonso A, Chung MK et al. Common variants in KCNN3 are associated with lone atrial fibrillation. Nat Genet 2010;42:240 –244. 6. Hsueh CH, Chang PC, Hsieh YC, Reher T, Chen PS, Lin SF. Proarrhythmic effect of blocking the small conductance calcium activated potassium channel in isolated canine left atrium. Heart Rhythm 2013;10:891 – 898. 7. Yu T, Wu RB, Guo HM, Deng CY, Zheng SY, Kuang SJ. Expression and functional role of small conductance Ca(2+)-activated K(+) channels in human atrial myocytes. Nan Fang Yi Ke Da Xue Xue Bao 2011;31:490 – 494. 8. Nagy N, Szuts V, Horva´th Z, Sepre´nyi G, Farkas AS, Acsai K et al. Does smallconductance calcium-activated potassium channel contribute to cardiac repolarization? J Mol Cell Cardiol 2009;47:656 –663. 9. Qi XY, Diness JG, Brundel B, Zhou XB, Naud P, Wu CT et al. Role of small conductance calcium-activated potassium channels in atrial electrophysiology and fibrillation in the dog. Circulation 2013 (Epub ahead of print) 10. Diness JG, Sørensen US, Nissen JD, Al-Shahib B, Jespersen T, Grunnet M et al. Inhibition of small-conductance Ca2+-activated K+ channels terminates and protects against atrial fibrillation. Circ Arrhythm Electrophysiol 2010;3:380 –390. 11. Diness JG, Skibsbye L, Jespersen T, Bartels ED, Sørensen US, Hansen RS et al. Effects on atrial fibrillation in aged hypertensive rats by Ca(2+)-activated K(+) channel inhibition. Hypertension 2011;57:1129 –1135. 12. Li N, Timofeyev V, Tuteja D, Xu D, Lu L, Zhang Q et al. Ablation of a Ca2+-activated K+ channel (SK2 channel) results in action potential prolongation in atrial myocytes and atrial fibrillation. J Physiol 2009;587(Pt 5):1087 –1100. 13. Zhang X-D, Timofeyev V, Li N, Myers RE, Zhang D, Singapuri A et al. Critical roles of a small conductance Ca2+-activated K+ channel (SK3) in the repolarization process of atrial myocytes. Cardiovasc Res 2014;101:317–325. 14. Mahida S, Mills R, Tucker NR, Simonson B, Macri V, Lemoine MD et al. Overexpression of KCNN3 results in sudden cardiac death. Cardiovasc Res 2014;101:326 –334. 15. Bond CT, Sprengel R, Bissonnette JM, Kaufmann WA, Pribnow D, Neelands T et al. Respiration and parturition affected by conditional overexpression of the Ca2+-activated K+ channel subunit, SK3. Science 2000;289:1942 –1946. Downloaded from by guest on November 15, 2015
Cardiovascular Research (2014) 101, 185–186 doi:10.1093/cvr/cvt340
Calcium-dependent potassium channels in the heart: clarity and confusion Stanley Nattel* and Xiao Yan Qi Department of Medicine and Research Center, Montreal Heart Institute and Universite´ de Montre´al, 5000 Belanger Street E, Montreal, Quebec, Canada H1 T 1C8 Online publish-ahead-of-print 2 January 2014
This editorial refers to ‘Critical roles of a small conductance Ca21-activated K1 channel (SK3) in the repolarization process of atrial myocytes’ by X.-D. Zhang et al., pp. 317–325, this issue and ‘Overexpression of KCNN3 results in sudden cardiac death’ by S. Mahida et al., pp. 326–334, this issue.
The opinions expressed in this article are not necessarily those of the Editors of Cardiovascular Research or of the European Society of Cardiology.
* Corresponding author. Tel: +1 514 376 3330; fax: +1 514 593 2493, Email: [email protected] Published on behalf of the European Society of Cardiology. All rights reserved. & The Author 2014. For permissions please email: [email protected].
Downloaded from by guest on November 15, 2015
Small-conductance Ca2+-activated K+ (SK) channels are widely expressed throughout the body. After initial dismissal of a functional role for SK channels in the heart,1 a series of elegant studies from the Chiamvimonvat laboratory rekindled interest,2,3 as did subsequent work suggesting that tachypaced rabbit pulmonary veins have enhanced SK channel trafficking to the cell membrane that accelerates repolarization.4 The field really got a boost from GWAS evidence, suggesting that variants in the KCNN3 gene encoding SK isoform 3 (SK3) channels are associated with the risk of atrial fibrillation (AF) in man.5 Questions remain, however, about the functional role that SK currents play in cardiac electrophysiology. Recent studies of SK channels in native tissues have provided conflicting results. Two investigations using apamin to block SK currents showed increased action potential duration (APD) of atrial cells,6,7 while one did not observe an effect.8 Recent detailed studies with a novel selective SK channel blocker provide insight into this variability, by showing that the expression of SK current in the canine atrium is regionally determined and increased by AF-related remodelling, such that statistically non-significant APD effects of SK channel block are seen in control left-atrium, with larger statistically significant effects in pulmonary vein cardiomyocyte sleeves and still larger effects in the presence of AF-related remodelling.9 There are also discrepant findings regarding the effects of SK blockade on atrial arrhythmias: several studies have shown clear anti-arrhyhmic actions,9 – 11 while one paper reported pro-arrhythmic effects.6 Experiments in genetically engineered mouse models have the potential to provide information about the functional importance of ion-channel subunits without the concerns about specificity that plague pharmacological studies. Li et al.12 took advantage of SK2 knockout mice to show a significant role of mice in murine atrial repolarization, as well as enhanced susceptibility of such mice to AF induction. In the present issue, two groups of investigators exploit genetically engineered mice to gain insights into the role of SK3 channels in cardiac electrical function.13,14 Both groups use SK3 overexpression to study
effects on electrophysiology and survival; in addition, Zhang et al.13 use an inducible genetic knockdown system to suppress native SK-3 expression; the Zhang et al.13 study focuses primarily on atrial properties and the Mahida study on ventricular consequences.14 Zhang et al.13 show that SK3 overexpression abbreviates atrial APD and facilitates AF induction, consistent with the majority of pharmacological studies of the role of atrial SK current.7,9 – 11 Genetic SK knockdown had inconsistent effects: APD was not significantly altered, pointing to no significant role of the SK3 subunit in repolarization; however, the apamin effect to prolong APD seen in wild-type and SK3 overexpressing mice was abolished by SK3 knockdown, suggesting a contribution.13 Zhang et al. found substantial premature mortality in SK3 overexpressing mice. The Mahida study complements and extends that of Zhang et al. Heart block and bradyarrhythmias were shown to occur in SK3 overexpressing mice at the time of premature death.14 While at 1 month SK3 overexpressing mice showed inducible atrial arrhythmias and a reduced AV nodal refractory period, at 3 months AV nodal refractoriness tended to be slightly prolonged. Moderate ventricular conduction slowing and increased ventricular APD dispersion were seen in 1-month mice, with enlargement and disorganization of the AV node at 5 months. These studies clarify some questions but raise new ones about the functional role of SK subunits in the heart. At the atrial level, the findings support the idea that SK channels contribute to atrial repolarization and that loss of SK function promotes AF susceptibility. At the ventricular and AV nodal level, the results are quite thought provoking and challenging. Why does SK3 overexpression promote premature death? The simplest explanation of the observations is that SK3 overexpression causes conduction disturbances and bradycardic death. However, it is not clear why increasing repolarizing K+-current should slow conduction. Furthermore, ventricular conduction slowing was modest and AV nodal function was not significantly affected. Could disturbed AV nodal structure have affected AV nodal function enough to cause highgrade block and bradycardic death? AV nodal histology was examined in 5-month-old mice, whereas premature death began at 30 days. It would have been interesting to know what happened to AV node histology at an earlier time-point. The disrupted AV nodal histology is in itself quite interesting: could SK3 overexpression have produced this by disturbing function in some cell-type other than adult cardiomyocytes (e.g. fibroblasts, cardiomyocyte precursors, leucocytes, neural
186 elements)? Finally, could bradycardia have been a result of the terminal event, rather than the cause? SK3 overexpression causes abnormal central control of respiration in mice15: could the deaths have been respiratory in origin, with bradycardia/AV block an agonal cardiac response? Clearly, we need to learn much more about the role of SK channel function in the cardiovascular system. As Alice said in Wonderland: ‘Curioser and curioser’! Conflict of interest: none declared.
Funding Canadian Institutes of Health Research (grants 6957, 44365, 68929); Heart and Stroke Foundation of Canada.
References 1. Eisner DA, Vaughan-Jones RD. Do calcium-activated potassium channels exist in the heart? Cell Calcium 1983;4:371 –386. 2. Xu Y, Tuteja D, Zhang Z, Xu D, Zhang Y, Rodriguez J et al. Molecular identification and functional roles of a Ca2+-activated K+ channel in human and mouse hearts. J Biol Chem 2003;278:49085 –49094. 3. Tuteja D, Xu D, Timofeyev V, Lu L, Sharma D, Zhang Z et al. Differential expression of small-conductance Ca2+-activated K+ channels SK1, SK2, and SK3 in mouse atrial and ventricular myocytes. Am J Physiol Heart Circ Physiol 2005;289:H2714 –H2723. 4. Ozgen N, Dun W, Sosunov EA, Anyukhovsky EP, Hirose M, Duffy HS et al. Early electrical remodeling in rabbit pulmonary vein results from trafficking of intracellular sk2 channels to membrane sites. Cardiovasc Res 2007;75:758–769.
Editorial
5. Ellinor PT, Lunetta KL, Glazer NL, Pfeufer A, Alonso A, Chung MK et al. Common variants in KCNN3 are associated with lone atrial fibrillation. Nat Genet 2010;42:240 –244. 6. Hsueh CH, Chang PC, Hsieh YC, Reher T, Chen PS, Lin SF. Proarrhythmic effect of blocking the small conductance calcium activated potassium channel in isolated canine left atrium. Heart Rhythm 2013;10:891 – 898. 7. Yu T, Wu RB, Guo HM, Deng CY, Zheng SY, Kuang SJ. Expression and functional role of small conductance Ca(2+)-activated K(+) channels in human atrial myocytes. Nan Fang Yi Ke Da Xue Xue Bao 2011;31:490 – 494. 8. Nagy N, Szuts V, Horva´th Z, Sepre´nyi G, Farkas AS, Acsai K et al. Does smallconductance calcium-activated potassium channel contribute to cardiac repolarization? J Mol Cell Cardiol 2009;47:656 –663. 9. Qi XY, Diness JG, Brundel B, Zhou XB, Naud P, Wu CT et al. Role of small conductance calcium-activated potassium channels in atrial electrophysiology and fibrillation in the dog. Circulation 2013 (Epub ahead of print) 10. Diness JG, Sørensen US, Nissen JD, Al-Shahib B, Jespersen T, Grunnet M et al. Inhibition of small-conductance Ca2+-activated K+ channels terminates and protects against atrial fibrillation. Circ Arrhythm Electrophysiol 2010;3:380 –390. 11. Diness JG, Skibsbye L, Jespersen T, Bartels ED, Sørensen US, Hansen RS et al. Effects on atrial fibrillation in aged hypertensive rats by Ca(2+)-activated K(+) channel inhibition. Hypertension 2011;57:1129 –1135. 12. Li N, Timofeyev V, Tuteja D, Xu D, Lu L, Zhang Q et al. Ablation of a Ca2+-activated K+ channel (SK2 channel) results in action potential prolongation in atrial myocytes and atrial fibrillation. J Physiol 2009;587(Pt 5):1087 –1100. 13. Zhang X-D, Timofeyev V, Li N, Myers RE, Zhang D, Singapuri A et al. Critical roles of a small conductance Ca2+-activated K+ channel (SK3) in the repolarization process of atrial myocytes. Cardiovasc Res 2014;101:317–325. 14. Mahida S, Mills R, Tucker NR, Simonson B, Macri V, Lemoine MD et al. Overexpression of KCNN3 results in sudden cardiac death. Cardiovasc Res 2014;101:326 –334. 15. Bond CT, Sprengel R, Bissonnette JM, Kaufmann WA, Pribnow D, Neelands T et al. Respiration and parturition affected by conditional overexpression of the Ca2+-activated K+ channel subunit, SK3. Science 2000;289:1942 –1946. Downloaded from by guest on November 15, 2015
