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Biomarkers of inflammation in persons with chronic tetraplegia
This content has been downloaded from IOPscience. Please scroll down to see the full text. 2015 J. Breath Res. 9 036001 (http://iopscience.iop.org/1752-7163/9/3/036001) View the table of contents for this issue, or go to the journal homepage for more
Download details: IP Address: 137.99.31.134 This content was downloaded on 18/05/2015 at 16:19
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J. Breath Res. 9 (2015) 036001
doi:10.1088/1752-7155/9/3/036001
Paper
Biomarkers of inflammation in persons with chronic tetraplegia received
24 November 2014 re vised
19 February 2015 accep ted for publication
5 March 2015 published
6 May 2015
Miroslav Radulovic1,2,3, William A Bauman1,2,3,4, Jill M Wecht1,2,3,4, Michael LaFountaine1, Nighat Kahn1, Joshua Hobson1, Kamaldeep Singh1, Christopher Renzi1, Christina Yen1 and Gregory J Schilero1,2,3 1
Rehabilitation Research and Development Center of Excellence for the Medical Consequences of Spinal Cord Injury, The James J. Peters VA Medical Center, Bronx, NY 10468, USA 2 Medical Service, The James J. Peters VA Medical Center, Bronx, NY 10468, USA 3 Department of Medicine, The Icahn School of Medicine at Mount Sinai, New York, NY 10029, USA 4 Department of Rehabilitation Medicine, The Icahn School of Medicine at Mount Sinai, New York, NY 10029, USA E-mail: [email protected] Keywords: spinal cord injury, tetraplegia, oxidative stress, asthma
Abstract In addition to lung volume restriction, individuals with chronic tetraplegia exhibit reduced airway caliber and bronchodilator responsiveness similar to persons with asthma. In asthma, airflow obstruction is closely linked to airway inflammation. Conversely, little is known regarding the airway inflammatory response in tetraplegia. To compare levels of biomarkers of inflammation in exhaled breath condensate (EBC) and serum in subjects with chronic tetraplegia, mild asthma, and able-bodied controls. Prospective, observational pilot study. Thirty-four subjects participated: tetraplegia (n = 12), asthma (n = 12), controls (n = 10). Biomarkers in EBC [8-isoprostane (8-IP), leukotriene B4 (LT-B4), prostaglandin E2 (PG-E2), tumor necrosis factor alpha (TNF-α), interleukin 6 (IL-6)] and serum (8-IP, LT-B4, TNF-α, IL-6) were determined using commercially available EIA kits (Cayman Chemical Company, Ann Arbor, MI). Separate, one-way ANOVA with Bonferroni’s post-hoc analyses were performed to determine group differences in demographic and dependent variables [EBC and serum biomarkers, fractional exhaled nitric oxide (FeNO), pulmonary function parameters, and specific airway conductance (sGaw)]. The tetraplegia group had significantly elevated 8-IP levels in EBC compared to the asthma (68 ± 38 versus 21 ± 13 pg ml−1; p
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My IOPscience
Biomarkers of inflammation in persons with chronic tetraplegia
This content has been downloaded from IOPscience. Please scroll down to see the full text. 2015 J. Breath Res. 9 036001 (http://iopscience.iop.org/1752-7163/9/3/036001) View the table of contents for this issue, or go to the journal homepage for more
Download details: IP Address: 137.99.31.134 This content was downloaded on 18/05/2015 at 16:19
Please note that terms and conditions apply.
J. Breath Res. 9 (2015) 036001
doi:10.1088/1752-7155/9/3/036001
Paper
Biomarkers of inflammation in persons with chronic tetraplegia received
24 November 2014 re vised
19 February 2015 accep ted for publication
5 March 2015 published
6 May 2015
Miroslav Radulovic1,2,3, William A Bauman1,2,3,4, Jill M Wecht1,2,3,4, Michael LaFountaine1, Nighat Kahn1, Joshua Hobson1, Kamaldeep Singh1, Christopher Renzi1, Christina Yen1 and Gregory J Schilero1,2,3 1
Rehabilitation Research and Development Center of Excellence for the Medical Consequences of Spinal Cord Injury, The James J. Peters VA Medical Center, Bronx, NY 10468, USA 2 Medical Service, The James J. Peters VA Medical Center, Bronx, NY 10468, USA 3 Department of Medicine, The Icahn School of Medicine at Mount Sinai, New York, NY 10029, USA 4 Department of Rehabilitation Medicine, The Icahn School of Medicine at Mount Sinai, New York, NY 10029, USA E-mail: [email protected] Keywords: spinal cord injury, tetraplegia, oxidative stress, asthma
Abstract In addition to lung volume restriction, individuals with chronic tetraplegia exhibit reduced airway caliber and bronchodilator responsiveness similar to persons with asthma. In asthma, airflow obstruction is closely linked to airway inflammation. Conversely, little is known regarding the airway inflammatory response in tetraplegia. To compare levels of biomarkers of inflammation in exhaled breath condensate (EBC) and serum in subjects with chronic tetraplegia, mild asthma, and able-bodied controls. Prospective, observational pilot study. Thirty-four subjects participated: tetraplegia (n = 12), asthma (n = 12), controls (n = 10). Biomarkers in EBC [8-isoprostane (8-IP), leukotriene B4 (LT-B4), prostaglandin E2 (PG-E2), tumor necrosis factor alpha (TNF-α), interleukin 6 (IL-6)] and serum (8-IP, LT-B4, TNF-α, IL-6) were determined using commercially available EIA kits (Cayman Chemical Company, Ann Arbor, MI). Separate, one-way ANOVA with Bonferroni’s post-hoc analyses were performed to determine group differences in demographic and dependent variables [EBC and serum biomarkers, fractional exhaled nitric oxide (FeNO), pulmonary function parameters, and specific airway conductance (sGaw)]. The tetraplegia group had significantly elevated 8-IP levels in EBC compared to the asthma (68 ± 38 versus 21 ± 13 pg ml−1; p
