Appl Biochem Biotechnol (2014) 172:2197–2204 DOI 10.1007/s12010-013-0651-y
Bioconversion of Corncob Acid Hydrolysate into Microbial Oil by the Oleaginous Yeast Lipomyces starkeyi Chao Huang & Xue-Fang Chen & Xiao-Yan Yang & Lian Xiong & Xiao-Qing Lin & Juan Yang & Bo Wang & Xin-De Chen
Received: 30 July 2013 / Accepted: 15 November 2013 / Published online: 17 December 2013 # Springer Science+Business Media New York 2013
Abstract For the first time, corncob acid hydrolysate was used for microbial oil production by the oleaginous yeast Lipomyces starkeyi. After hydrolysis by dilute sulfuric acid, corncob could turn into an acid hydrolysate with a sugar concentration of about 42.3 g/L. Detoxified by overliming and absorption with activated carbon, the corncob hydrolysate could be used by L. starkeyi efficiently that a total biomass of 17.2 g/L with a lipid content of 47.0 % (corresponding to a lipid yield of 8.1 g/L) and a lipid coefficient of 20.9 could be obtained after cultivation on the corncob hydrolysate for 8 days. Therefore, L. starkeyi is a promising strain for microbial oil production from lignocellulosic biomass. Glucose and xylose were used by L. starkeyi simultaneously during lipid fermentation while arabinose could not be utilized by it. Besides, the lipid composition of L. starkeyi was similar to that of vegetable oils; thus, it is a promising feedstock for biodiesel production. Keywords Lipomyces starkeyi . Microbial oil . Corncob acid hydrolysate . Biodiesel
Introduction Recently, microbial oil is considered as one attractive lipid feedstock for biodiesel production due to its advantages such as a short life cycle; less required labor; little limited by location,
Chao Huang and Xue-Fang Chen have the same contribution and are co-first authors.
C. Huang : L. Xiong : X.
Bioconversion of Corncob Acid Hydrolysate into Microbial Oil by the Oleaginous Yeast Lipomyces starkeyi Chao Huang & Xue-Fang Chen & Xiao-Yan Yang & Lian Xiong & Xiao-Qing Lin & Juan Yang & Bo Wang & Xin-De Chen
Received: 30 July 2013 / Accepted: 15 November 2013 / Published online: 17 December 2013 # Springer Science+Business Media New York 2013
Abstract For the first time, corncob acid hydrolysate was used for microbial oil production by the oleaginous yeast Lipomyces starkeyi. After hydrolysis by dilute sulfuric acid, corncob could turn into an acid hydrolysate with a sugar concentration of about 42.3 g/L. Detoxified by overliming and absorption with activated carbon, the corncob hydrolysate could be used by L. starkeyi efficiently that a total biomass of 17.2 g/L with a lipid content of 47.0 % (corresponding to a lipid yield of 8.1 g/L) and a lipid coefficient of 20.9 could be obtained after cultivation on the corncob hydrolysate for 8 days. Therefore, L. starkeyi is a promising strain for microbial oil production from lignocellulosic biomass. Glucose and xylose were used by L. starkeyi simultaneously during lipid fermentation while arabinose could not be utilized by it. Besides, the lipid composition of L. starkeyi was similar to that of vegetable oils; thus, it is a promising feedstock for biodiesel production. Keywords Lipomyces starkeyi . Microbial oil . Corncob acid hydrolysate . Biodiesel
Introduction Recently, microbial oil is considered as one attractive lipid feedstock for biodiesel production due to its advantages such as a short life cycle; less required labor; little limited by location,
Chao Huang and Xue-Fang Chen have the same contribution and are co-first authors.
C. Huang : L. Xiong : X.
