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Mol Neurobiol. Author manuscript; available in PMC 2017 September 01. Published in final edited form as: Mol Neurobiol. 2016 September ; 53(7): 4387–4396. doi:10.1007/s12035-015-9347-3.
Autologous bone marrow mononuclear cell transplantation delays progression of carotid atherosclerosis in rabbits Kefei Cuia,*, Xiao Maa, Lie Yub, Chao Jiangb,c, Chao Fua, Xiaojie Fub, Xiaofang Yua, Yuanjing Huanga, Suyun Houa, Caifeng Sia, Zhengguang Chena, Jing Yua, Jieru Wanc, and Jian Wangc,* aDepartment
Author Manuscript
of Ultrasound, The First Affiliated Hospital of Zhengzhou University, Zhengzhou 450052, China
bDepartment
of Neurology, The Fifth Affiliated Hospital of Zhengzhou University, Zhengzhou, 450052 Henan, China cDepartment
of Anesthesiology and Critical Care Medicine, Johns Hopkins University, School of Medicine, Baltimore, MD, USA
Abstract Background—Bone marrow mononuclear cells (BMMNCs) can counteract oxidative stress and inhibit the inflammatory response in focal ischemic stroke models. However, the effect of BMMNC transplantation on carotid atherosclerosis needs to be determined.
Author Manuscript
Methods—The carotid atherosclerotic plaque model was established in New Zealand White rabbits by balloon injury and 8 weeks of high-fat diet. Rabbits were randomized to receive an intravenous injection of autologous BrdU-labeled BMMNCs or an equal volume of phosphatebuffered saline. Plaques were evaluated for expression of proinflammatory and anti-inflammatory cytokines, antioxidant proteins, and markers of cell death.
Author Manuscript
Results—BMMNCs migrated into atherosclerotic plaque on the first day after cell transplantation. BMMNC-treated rabbits had smaller plaques and more collagen deposition than did the vehicle-treated controls on day 28 (p
Mol Neurobiol. Author manuscript; available in PMC 2017 September 01. Published in final edited form as: Mol Neurobiol. 2016 September ; 53(7): 4387–4396. doi:10.1007/s12035-015-9347-3.
Autologous bone marrow mononuclear cell transplantation delays progression of carotid atherosclerosis in rabbits Kefei Cuia,*, Xiao Maa, Lie Yub, Chao Jiangb,c, Chao Fua, Xiaojie Fub, Xiaofang Yua, Yuanjing Huanga, Suyun Houa, Caifeng Sia, Zhengguang Chena, Jing Yua, Jieru Wanc, and Jian Wangc,* aDepartment
Author Manuscript
of Ultrasound, The First Affiliated Hospital of Zhengzhou University, Zhengzhou 450052, China
bDepartment
of Neurology, The Fifth Affiliated Hospital of Zhengzhou University, Zhengzhou, 450052 Henan, China cDepartment
of Anesthesiology and Critical Care Medicine, Johns Hopkins University, School of Medicine, Baltimore, MD, USA
Abstract Background—Bone marrow mononuclear cells (BMMNCs) can counteract oxidative stress and inhibit the inflammatory response in focal ischemic stroke models. However, the effect of BMMNC transplantation on carotid atherosclerosis needs to be determined.
Author Manuscript
Methods—The carotid atherosclerotic plaque model was established in New Zealand White rabbits by balloon injury and 8 weeks of high-fat diet. Rabbits were randomized to receive an intravenous injection of autologous BrdU-labeled BMMNCs or an equal volume of phosphatebuffered saline. Plaques were evaluated for expression of proinflammatory and anti-inflammatory cytokines, antioxidant proteins, and markers of cell death.
Author Manuscript
Results—BMMNCs migrated into atherosclerotic plaque on the first day after cell transplantation. BMMNC-treated rabbits had smaller plaques and more collagen deposition than did the vehicle-treated controls on day 28 (p
