0013-7227/78/1026-1909$02.00/0 Endocrinology Copyright © 1978 by The Endocrine Society
Vol. 102, No. 6 Printed in U.S.A.
Antiserum to Somatostatin Reverses Starvation-Induced Inhibition of Growth Hormone but Not Insulin Secretion* GLORIA SHAFFER TANNENBAUM.f JACQUES EPELBAUM,| ELEANOR COLLE, PAUL BRAZEAU,§ AND JOSEPH B. MARTINf Departments of Neurology and Neurosurgery, and Pediatrics, McGill University, Montreal, Quebec, Canada ABSTRACT. The role of SRIF in starvation-induced inhibition of GH and insulin secretion was assessed by passive immunization with anti-SRIF serum. Six-hour secretory profiles obtained from chronically cannulated male rats deprived of food for 72 h showed marked suppression of GH secretory bursts and significant depression of plasma insulin levels. Administration of 1 ml SRIF antiserum (SRIF AS) iv to starved rats resulted in rapid (within 15 min) restoration of high amplitude GH pulses (600-800 ng/ml) and significant elevation of GH trough values. The mean 6-h GH level of starved SRIF, AS-treated rats (189.2 ± 23.9 ng/ml) was signifi-
G
ROWTH HORMONE (GH) secretion in the rat is characterized by an endogenous ultradian rhythm with major episodes of GH secretion occurring at approximately 3.3h intervals throughout a 24-h period. Plasma GH concentrations reach levels of 400-800 ng/ml during a secretory burst and fall to undetectable levels (600 ng/ml and trough values
Vol. 102, No. 6 Printed in U.S.A.
Antiserum to Somatostatin Reverses Starvation-Induced Inhibition of Growth Hormone but Not Insulin Secretion* GLORIA SHAFFER TANNENBAUM.f JACQUES EPELBAUM,| ELEANOR COLLE, PAUL BRAZEAU,§ AND JOSEPH B. MARTINf Departments of Neurology and Neurosurgery, and Pediatrics, McGill University, Montreal, Quebec, Canada ABSTRACT. The role of SRIF in starvation-induced inhibition of GH and insulin secretion was assessed by passive immunization with anti-SRIF serum. Six-hour secretory profiles obtained from chronically cannulated male rats deprived of food for 72 h showed marked suppression of GH secretory bursts and significant depression of plasma insulin levels. Administration of 1 ml SRIF antiserum (SRIF AS) iv to starved rats resulted in rapid (within 15 min) restoration of high amplitude GH pulses (600-800 ng/ml) and significant elevation of GH trough values. The mean 6-h GH level of starved SRIF, AS-treated rats (189.2 ± 23.9 ng/ml) was signifi-
G
ROWTH HORMONE (GH) secretion in the rat is characterized by an endogenous ultradian rhythm with major episodes of GH secretion occurring at approximately 3.3h intervals throughout a 24-h period. Plasma GH concentrations reach levels of 400-800 ng/ml during a secretory burst and fall to undetectable levels (600 ng/ml and trough values
