J Huazhong Univ Sci Technol [Med Sci] 34(1):131-136,2014 10.1007/s11596-014-1244-x J DOI Huazhong Univ Sci Technol [Med Sci] 34(1):2014
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Antioxidant and Antigenotoxic Activity of Bioactive Extracts from Corn Tassel* Li-chun WANG (王丽春)†, Ya-qin YU (余雅芹)†, Min FANG (方 敏), Cai-gui ZHAN (占才贵), Hong-yan PAN (潘红艳), Yong-ning WU (吴永宁), Zhi-yong GONG (宫智勇)# Institute of Food Science and Engineering, Wuhan Polytechnic University, Wuhan 430023, China © Huazhong University of Science and Technology and Springer-Verlag Berlin Heidelberg 2014
Summary: This study is designed to evaluate antioxidant and antigenotoxic activities of corn tassel extracts (CTTs). The major bioactive components of CTTs include flavonoid, saponin and polysaccharide. The antioxidant properties of the three bioactive components of CTTs were investigated by Ferric Reducing Antioxidant Property (FRAP) and 1, 1-diphenyl-2-picrylhydrazyl (DPPH) assays. The activities of the extracts were determined by assessing the inhibition of mutagenicity of the direct-acting mutagen fenaminosulf, sodium azide, and indirect-acting mutagen 2-aminofluorene using the Ames test (strains TA98 and TA100). The results showed that the extraction rates of flavonoid, saponin, and polysaccharide from the dried corn tassels were 1.67%, 2.41% and 4.76% respectively. DPPH and FRAP assay strongly demonstrated that CTTs had antioxidant properties. CTTs at doses of 625, 1250 and 2500 μg per plate reduced 2-aminofluorene mutagenicity by 12.52%, 28.76% and 36.49% in Salmonella typhimurium TA98 strain assay respectively and by 10.98%, 25.27% and 37.83%, at the same doses in Salmonella typhimurium TA100 assay system, respectively. 3-[4, 5-dimethylthiazol-2-yl]-2, 5-diphenyltetrazolium bromide (MTT) assay showed that the different concentrations of CTTs inhibited the proliferation of MGC80-3 cells in a dose-dependent manner (P
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Antioxidant and Antigenotoxic Activity of Bioactive Extracts from Corn Tassel* Li-chun WANG (王丽春)†, Ya-qin YU (余雅芹)†, Min FANG (方 敏), Cai-gui ZHAN (占才贵), Hong-yan PAN (潘红艳), Yong-ning WU (吴永宁), Zhi-yong GONG (宫智勇)# Institute of Food Science and Engineering, Wuhan Polytechnic University, Wuhan 430023, China © Huazhong University of Science and Technology and Springer-Verlag Berlin Heidelberg 2014
Summary: This study is designed to evaluate antioxidant and antigenotoxic activities of corn tassel extracts (CTTs). The major bioactive components of CTTs include flavonoid, saponin and polysaccharide. The antioxidant properties of the three bioactive components of CTTs were investigated by Ferric Reducing Antioxidant Property (FRAP) and 1, 1-diphenyl-2-picrylhydrazyl (DPPH) assays. The activities of the extracts were determined by assessing the inhibition of mutagenicity of the direct-acting mutagen fenaminosulf, sodium azide, and indirect-acting mutagen 2-aminofluorene using the Ames test (strains TA98 and TA100). The results showed that the extraction rates of flavonoid, saponin, and polysaccharide from the dried corn tassels were 1.67%, 2.41% and 4.76% respectively. DPPH and FRAP assay strongly demonstrated that CTTs had antioxidant properties. CTTs at doses of 625, 1250 and 2500 μg per plate reduced 2-aminofluorene mutagenicity by 12.52%, 28.76% and 36.49% in Salmonella typhimurium TA98 strain assay respectively and by 10.98%, 25.27% and 37.83%, at the same doses in Salmonella typhimurium TA100 assay system, respectively. 3-[4, 5-dimethylthiazol-2-yl]-2, 5-diphenyltetrazolium bromide (MTT) assay showed that the different concentrations of CTTs inhibited the proliferation of MGC80-3 cells in a dose-dependent manner (P
