Tohoku
J. exp.
Med.,
1977,
122, 299-300 Short
Antibacterial
Activity
TAKUSEI UMENAI,
Detected
in
TASTUYA SASAKI
Report
Duodenal
and
Juice
TASUKE
KONNO
Central Clinical Laboratory and Department of Pediatrics , Tohoku University School of Medicine, Sendai 980
UMENAI, T., SASAKI, T., and Konuo, T. Antibacterial Activity Detected in Duodenal Juice. Tohoku J. exp. Med., 1977, 122 (3), 299-300 Antibacterial activity could be detected in bacterium-negative duodenal juice from 3 patients, whereas it was not detected in bacterium-positive duodenal juice from other 3 patients. The follow-up study revealed that appearance of the activity in duodenal juice was just coincident with disappearance of bacteria in duodenal juice. The activity was not influenced by pH of the duodenal juice. These results seem to suggest that some antibacterial activity, different from that of pH, in duodenal juice regulates in some way proliferation of the upper gastrointestinal flora. - anti-infective agents; bacterial infection; gastrointestinal disease The et
al.
motility
1969)
tinal
be
presence
We
report
by 6
with
adult
for
of
hr,
were less
identified.
than
20
examined
per
for
TABLE 1.
the
ml.
of the
bacteria in
activity
disorder
stimulated juice
spread number
The
acidity of
activity
alimentary
gastric
was
the
in
gastric the
duodenal
of
the
juice
for
(Drasar
gastrointes has
duodenal
not
juice
sample
Filtered
antibacterial
duodenal
with
into
duodenal
of was through activity
a
of
blood
agar
colonies
by
as
and
been
which
the
disc-agar
collected Care
samples
can
were
when
membrane diffusion
the
tested
method
for
incubated
the
content
(0.45 ƒÊm),
to
10-fold
and
and
a
taken
diluted
agar
counted
with
was
serially
Drigalskis was
negative
millipore
The sample,
formed
taken a
the
was
secretin.
juice.
milliliter on
juice
porcine
bacteria of
the as
bacteria samples described
Bacteria and antibacterial activity of duodenal juice from adult patients
* Difference of radius between the inhibition zone of bacteria growth mm): -, less than 1 mm; +, 1-2 mm; ??, more than 2 mm. Received
juice
upper
method.
after
the
and
antibacterial
One-twentieth
then
1967)
proliferation
antibacterial the
with tube
saline, 24
of
patients
al.
inhibit
sensitive
duodenal
physiological
detected
were
simple,
et to
here
concentration.
37•Ž
was
considered
contamination
bacterium
at
a
lumen
prevent
(Gorbach
the
far.
detected From
gut
but so
double
the
been
tract,
reported
of
have
publication,
March
10,
1977. 299
and the disc (4
300
T. Umenai
by Umenai and Koriyama (1976). Kledsiella (U-51-1), isolated from the urine of a leukemic patient, was used as the test bacterium throughout the experiment. Duodenal juice from 3 patients was found to be contaminated with bacteria, in which antibacterial activity was not detected. On the other hand, significant antibacterial activity was present in the juice from the other 3 patients, from which no bacteria were isolated (Table 1). No correlation was observed among the presence of the activity, pH of the sample, and the underlying disease. In another series of experiments, 3 infants developing intractable diarrhea were examined for two months (Table 2). It was found that the antibacterial activity became detectable in the juice in 2 patients concomitant with improvement of the symptoms and disappearance of bacteria in duodenal juice. In the other patient, such an appearance of the activity was not seen and bacteria were isolated from the juice throughout the observation period. Again, pH of duodenal juice was observed to exert no influence on the activity. Taken together, these results suggest that some antibacterial activity in the duodenal juice controls the growth of the upper gastrointestinal flora.
* •õ
The Diarrhea
TABLE 2.
Bacteria and antibacterial activity of duodenal juice from infants
interval
sampling
of observed. •ö
was Diarrhea
about not
one observed
month
. .
References 1)
2)
Drasar, B.S., Shiner, M. & McLeold , G.M. (1969) Studies on the intestinal flora. 1: The bacteria] flora of the gastrointestinal tract in healthy and achlorhydric persons .. G a.stroenterology, 56, 71-79. Gorbach, S.L., Plaut, A.G., Nahas, L., Weinstein, L ., Spanknebel, G. & Levitan , R. St (1967) udies of intestinal microflora . 2: Microorganisms of the small intestine and their relation to oral and fecal flora . Gastroenterology, 53, 856-867 .3) U menai, T. & Koriyama, (1976) Bacterial examination of secretes from maxilla ry sinus. Jap. J. clip. Path. (Jap.), 24, suppl . 141.
J. exp.
Med.,
1977,
122, 299-300 Short
Antibacterial
Activity
TAKUSEI UMENAI,
Detected
in
TASTUYA SASAKI
Report
Duodenal
and
Juice
TASUKE
KONNO
Central Clinical Laboratory and Department of Pediatrics , Tohoku University School of Medicine, Sendai 980
UMENAI, T., SASAKI, T., and Konuo, T. Antibacterial Activity Detected in Duodenal Juice. Tohoku J. exp. Med., 1977, 122 (3), 299-300 Antibacterial activity could be detected in bacterium-negative duodenal juice from 3 patients, whereas it was not detected in bacterium-positive duodenal juice from other 3 patients. The follow-up study revealed that appearance of the activity in duodenal juice was just coincident with disappearance of bacteria in duodenal juice. The activity was not influenced by pH of the duodenal juice. These results seem to suggest that some antibacterial activity, different from that of pH, in duodenal juice regulates in some way proliferation of the upper gastrointestinal flora. - anti-infective agents; bacterial infection; gastrointestinal disease The et
al.
motility
1969)
tinal
be
presence
We
report
by 6
with
adult
for
of
hr,
were less
identified.
than
20
examined
per
for
TABLE 1.
the
ml.
of the
bacteria in
activity
disorder
stimulated juice
spread number
The
acidity of
activity
alimentary
gastric
was
the
in
gastric the
duodenal
of
the
juice
for
(Drasar
gastrointes has
duodenal
not
juice
sample
Filtered
antibacterial
duodenal
with
into
duodenal
of was through activity
a
of
blood
agar
colonies
by
as
and
been
which
the
disc-agar
collected Care
samples
can
were
when
membrane diffusion
the
tested
method
for
incubated
the
content
(0.45 ƒÊm),
to
10-fold
and
and
a
taken
diluted
agar
counted
with
was
serially
Drigalskis was
negative
millipore
The sample,
formed
taken a
the
was
secretin.
juice.
milliliter on
juice
porcine
bacteria of
the as
bacteria samples described
Bacteria and antibacterial activity of duodenal juice from adult patients
* Difference of radius between the inhibition zone of bacteria growth mm): -, less than 1 mm; +, 1-2 mm; ??, more than 2 mm. Received
juice
upper
method.
after
the
and
antibacterial
One-twentieth
then
1967)
proliferation
antibacterial the
with tube
saline, 24
of
patients
al.
inhibit
sensitive
duodenal
physiological
detected
were
simple,
et to
here
concentration.
37•Ž
was
considered
contamination
bacterium
at
a
lumen
prevent
(Gorbach
the
far.
detected From
gut
but so
double
the
been
tract,
reported
of
have
publication,
March
10,
1977. 299
and the disc (4
300
T. Umenai
by Umenai and Koriyama (1976). Kledsiella (U-51-1), isolated from the urine of a leukemic patient, was used as the test bacterium throughout the experiment. Duodenal juice from 3 patients was found to be contaminated with bacteria, in which antibacterial activity was not detected. On the other hand, significant antibacterial activity was present in the juice from the other 3 patients, from which no bacteria were isolated (Table 1). No correlation was observed among the presence of the activity, pH of the sample, and the underlying disease. In another series of experiments, 3 infants developing intractable diarrhea were examined for two months (Table 2). It was found that the antibacterial activity became detectable in the juice in 2 patients concomitant with improvement of the symptoms and disappearance of bacteria in duodenal juice. In the other patient, such an appearance of the activity was not seen and bacteria were isolated from the juice throughout the observation period. Again, pH of duodenal juice was observed to exert no influence on the activity. Taken together, these results suggest that some antibacterial activity in the duodenal juice controls the growth of the upper gastrointestinal flora.
* •õ
The Diarrhea
TABLE 2.
Bacteria and antibacterial activity of duodenal juice from infants
interval
sampling
of observed. •ö
was Diarrhea
about not
one observed
month
. .
References 1)
2)
Drasar, B.S., Shiner, M. & McLeold , G.M. (1969) Studies on the intestinal flora. 1: The bacteria] flora of the gastrointestinal tract in healthy and achlorhydric persons .. G a.stroenterology, 56, 71-79. Gorbach, S.L., Plaut, A.G., Nahas, L., Weinstein, L ., Spanknebel, G. & Levitan , R. St (1967) udies of intestinal microflora . 2: Microorganisms of the small intestine and their relation to oral and fecal flora . Gastroenterology, 53, 856-867 .3) U menai, T. & Koriyama, (1976) Bacterial examination of secretes from maxilla ry sinus. Jap. J. clip. Path. (Jap.), 24, suppl . 141.
